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Reduced Expression Of Top1beta Gene Induces Programmed Cell Death And Alters Ascorbate Metabolism In Daucus Carota Cultured Cells.

V. Locato, A. Balestrazzi, L. De Gara, D. Carbonera
Published 2006 · Medicine, Biology

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Topoisomerase I (topo I) is a nuclear enzyme which plays a fundamental role in several pathways involving changes in DNA topology. The topo I-mediated reaction is accomplished by the transient covalent binding of the enzyme to DNA (topo I-DNA complex). Stabilization of the topo I-DNA complex, leading to irreversible double-strand breaks, has been reported to occur in animal cells under oxidative stress conditions and during apoptosis. In order to study the existence of a putative link between the topo I-mediated DNA damage and ascorbate (ASC) metabolism, also involved in the responses against oxidative stress and in the apoptotic process in plants, Daucus carota cells showing reduced expression of the top1beta gene encoding the topo Ibeta isoform were produced, using an antisense RNA strategy. Two independent transgenic lines (AT1-beta/22 and beta/36), characterized by a slow growth phenotype, resistance to camptothecin, a specific inhibitor of topo I, but sensitivity to etoposide, an inhibitor of topo II, were investigated in this study. In the absence of external stimuli, AT1-beta/22 and beta/36 cells underwent programmed cell death (PCD) in a precocious phase of the growth curve. ASC metabolism showed remarkable differences in AT1-beta/22 and beta/36 cells, compared with control, and the observed alterations were similar to those occurring in tobacco Yellow Bright-2 cells induced to enter PCD by exogenous stimuli. However, differently from other studied examples of PCD, overproduction of reactive oxygen species was not detected in AT1-beta/22 and beta/36 cells. The relevance of these findings in relation to the signalling pathways leading to PCD is discussed.
This paper references
Reactive oxygen species production, impair
RA Vacca (2004)
Tobacco BY-2 Cells
T. Nagata (2004)
Ascorbate metabolism and plant growth from germination to cell death Vitamin C: its function and biochemistry in animals and plants
De Gara (2004)
Cloning of a cDNA encoding DNA topoisomerase I in Daucus carota and expression analysis in relation to cell proliferation.
A. Balestrazzi (1996)
The human topoisomerase I damage response plays a role in apoptosis.
K. Søe (2004)
Changes in gene expression during programmed cell death in tomato cell suspensions
F. Hoeberichts (2004)
Transformation and regeneration of carrot (Daucus carota L.)
M. Hardegger (2004)
Cellular roles of DNA topoisomerases: a molecular perspective
J. Wang (2002)
Cloning of yeast top 1 , the gene encoding DNA topoisomerase I , and construction of mutants defective in both DNA topoisomerase I and DNA topoisomerase II
T Goto (1985)
DNA topoisomerase I
R. Verheijen (1996)
Altered serine/arginine-rich protein phosphorylation and exonic enhancer-dependent splicing in Mammalian cells lacking topoisomerase I.
J. Soret (2003)
Induction of Reversible Complexes between Eukaryotic DNA Topoisomerase I and DNA-containing Oxidative Base Damages
P. Pourquier (1999)
Apoptotic Topoisomerase I-DNA Complexes Induced by Staurosporine-mediated Oxygen Radicals*
O. Sordet (2004)
Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection.
R. Mittler (1999)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Disruption of a DNA Topoisomerase I Gene Affects Morphogenesis in Arabidopsis Article, publication date, and citation information can be found at
T. Takahashi (2002)
Transgenic tobacco plants containing Bt and GNA genes
H. Zhang (2007)
Exopolysaccharides produced by plant pathogenic bacteria affect ascorbate metabolism in Nicotiana tabacum.
M. D. de Pinto (2003)
Cell death induced by topoisomerase-targeted
SH Kaufmann (1998)
Change in the redox state of glutathione regulates differentiation of tracheary elements in Zinnia cells and Arabidopsis roots.
K. Henmi (2005)
Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity.
C. Thrash (1984)
Transgenic tobacco plants
D Inzé (1999)
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
K. Asada (1999)
DNA topoisomerases. Annual Review of Biochemistry
Jc Wang (1996)
Hydrogen Peroxide Induces Topoisomerase I-mediated DNA Damage and Cell Death*
P. Daroui (2004)
Changes in the Antioxidant Systems as Part of the Signaling Pathway Responsible for the Programmed Cell Death Activated by Nitric Oxide and Reactive Oxygen Species in Tobacco Bright-Yellow 2 Cells1
M. D. de Pinto (2002)
Carrot cells contain two top1 genes having the coding capacity for two distinct DNA topoisomerases I.
A. Balestrazzi (2000)
Targeted disruption of the mouse topoisomerase I gene by camptothecin selection.
S. Morham (1996)
The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells
M. C. Pinto (2005)
Rapid isolation of high molecular weight plant DNA.
M. G. Murray (1980)
A rapid and sensitive method for the quanti
MM Bradford (1976)
Antioxidant responses to drought in sunflower and sorghum seedlings.
J. Zhang (1996)
Characterisation of two topoisomerase 1 genes in the pufferfish (Fugu rubripes).
S. Smith (2001)
Vitamin C : Its Functions and Biochemistry in Animals and Plants
H. Asard (2004)
Assay of excised
CM Wehr (1992)
Altered serine/arginine-rich protein
J Soret (2003)
Ascorbic acid deficiency activates
ME Alvarez (2005)
Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis1
Valeria R Pavet (2005)
Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column.
E. M. Park (1992)
Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in Tobacco
R. Mittler (1998)
tomato cell suspensions
H Hug (1994)
Systemic signalling and acclimation
P. Mullineaux (1999)
Cloning of a cDNA encoding DNA
D. Carbonera (1996)
DNA topoisomerase I is essential in Drosophila melanogaster.
M. Lee (1993)
protein–dye binding
F Carimi (2003)
Catalytic inhibitors of DNA topoisomer
T Andoh (1998)
Reactive oxygen species production, impairment of glucose oxidation and cytosolic ascorbate peroxidase are early events in heat-shock-induced programmed cell death in tobacco BY-2 cells
Ra Vacca (2004)
Rapid isolation of high mole
MG Murray (1980)
protein–dye binding
F Carimi (2003)
Nutrient requirement
OL Gamborg (1968)
The redox state
MC de Pinto (1999)
Regulation and function of ascorbate peroxidase isoenzymes.
S. Shigeoka (2002)
8-oxoguanine and 5-hydroxycytosine
S Shigeoka (2002)
Cytokinins: new apoptotic inducers in plants
F. Carimi (2002)
Isolation and functional analysis of the 5′-flanking region of carrot top1β gene coding for the β isoform of DNA topoisomerase I
A. Balestrazzi (2003)
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.
S. Karpiński (1999)
Cloning of yeast TOP1, the gene encoding DNA topoisomerase I, and construction of mutants defective in both DNA topoisomerase I and DNA topoisomerase II.
T. Goto (1985)
Extracellular H(2)O(2) induced by oligogalacturonides is not involved in the inhibition of the auxin-regulated rolB gene expression in tobacco leaf explants.
D. Bellincampi (2000)
Assay of excised
EM Park (1992)
Assaying chimeric genes in plants: The GUS gene fusion system
R. Jefferson (2007)
Nutrient requirements of suspension cultures of soybean root cells.
O. Gamborg (1968)
Cell death induced by topoisomerase-targeted drugs: more questions than answers.
S. Kaufmann (1998)
No requirement of reactive oxygen intermediates in Fas‐mediated apoptosis
H. Hug (1994)
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control.
G. Noctor (1998)
Catalytic inhibitors of DNA topoisomerase II.
T. Andoh (1998)
Reactive oxygen species production , impairment of glucose oxidation and cytosolic ascorbate peroxidase are early events in heatshockinduced programmed cell death in tobacco BY - 2 cells
RA Vacca (2004)
reactive oxygen species in tobacco BY-2 cells
OL Gamborg (1968)
Ascorbate and glutathione: keeping
G Noctor (1998)
The water-cycle in chloroplasts: scavenging
K. Asada (1999)
Cell death induced by topoisomerasetargeted drugs : more questions than answers
SH Kaufmann (1998)

This paper is referenced by
Genetic Transformation of Carrot (Daucus carota) and Other Apiaceae Species
R. Baranski (2008)
Cloning and characterization of DNA topoisomerase I gene Top1 from maize (Zea mays L.).
Yijun Wang (2010)
Redox homeostasis in plants. The challenge of living with endogenous oxygen production
L. Gara (2010)
Ultrastructural and Molecular Analyses Reveal Enhanced Nucleolar Activity in Medicago truncatula Cells Overexpressing the MtTdp2α Gene
Anca Macovei (2018)
Genetic Engineering of Carrot
Rafal Baranski (2019)
The Human Tyrosyl-DNA Phosphodiesterase 1 (hTdp1) Inhibitor NSC120686 as an Exploratory Tool to Investigate Plant Tdp1 Genes
Anca Macovei (2018)
Oligogalacturonides stimulate antioxidant system in alfalfa roots
D. Camejo (2012)
The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses
Maria Elisa Sabatini (2017)
SA and ROS are involved in methyl salicylate-induced programmed cell death in Arabidopsis thaliana
L. Yun (2011)
Elenco Parziale Prodotti di Ateneo
A. Olivieri (2006)
Ophiobolin A, a sesterterpenoid fungal phytotoxin, displays higher in vitro growth-inhibitory effects in mammalian than in plant cells and displays in vivo antitumor activity.
Marina Bury (2013)
Mutations in topoisomerase I as a self-resistance mechanism coevolved with the production of the anticancer alkaloid camptothecin in plants
S. Sirikantaramas (2008)
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
The tyrosyl-DNA phosphodiesterase gene family in Medicago truncatula Gaertn.: bioinformatic investigation and expression profiles in response to copper- and PEG-mediated stress
A. Macovei (2010)
Response to UV-C radiation in topo I-deficient carrot cells with low ascorbate levels.
A. Balestrazzi (2010)
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