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The Immunolocation Of A Xyloglucan Endotransglucosylase/hydrolase Specific To Elongating Tissues In Cicer Arietinum Suggests A Role In The Elongation Of Vascular Cells.
T. Jiménez, I. Martín, E. Labrador, B. Dopico
Published 2006 · Medicine, Biology
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In a previous work, a Cicer arietinum cDNA clone (CaXTH1) encoding a xyloglucan endotransglucosylase/hydrolase (XTH1) protein was isolated and characterized. CaXTH1 showed an expression pattern specific to growing tissue: mostly epicotyls and the upper growing internodes of adult stems. CaXTH1 mRNA was not detected in any other organs of either seedlings or adult plants, suggesting an involvement of the putative XTH encoded by CaXTH1 in the chickpea cell expansion process. After the generation of polyclonal antibodies by using the XTH1 recombinant protein and the analysis of the specificity of the antibodies for XTH proteins, here the specific location of the chickpea XTH1-cross-reacting protein in cell walls of epicotyls, radicles, and stems is reported, evaluated by western blot and immunocytochemical studies. The results indicate a function for this protein in the elongation of parenchyma cells of epicotyls and also in developing vascular tissue, suggesting a role in the elongation of vascular cells.
This paper references
Xyloglucan endotransglycosylase activity in pine hypocotyls. Intracellular localization and relationship with endogenous growth
C. Barrachina (1998)
Auxin regulation and spatial localization of an endo-1,4-beta-D-glucanase and a xyloglucan endotransglycosylase in expanding tomato hypocotyls.
C. Catalá (1997)
Endo-1,4-[beta]-Glucanase, Xyloglucanase, and Xyloglucan Endo-Transglycosylase Activities Versus Potential Substrates in Ripening Tomatoes
G. Maclachlan (1994)
Partial purification of cell wall b-galactosidase from Cicer arietinum epicotyls
B Dopico (1989)
Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule.
K. Nishitani (1992)
The XTH family
JKC Rose (2002)
Isolation and analysis of genes preferentially expressed during early cotton fibre development by subtractive PCR and cDNA arrays
S-H Ji (2003)
Expression of a xyloglucan endo-transglycosylase gene is closely related to grape berry softening
M. Ishimaru (2002)
XTH acts at the microfibril-matrix interface during cell elongation.
K. Vissenberg (2005)
Differential expression of AtXTH 17 ,18 ,19 and - 20 genes in Arabidopsis roots . Physiological roles in specification in cell wall construction
W Xu (2004)
An analysis of relative elemental growth rate, epidermal cell size and xyloglucan endotransglycosylase activity through the growing zone of ageing maize leaves
S. Palmer (1996)
Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels.
A. Shevchenko (1996)
Changes in xyloglucan endotransglycosylase (XET) activity during hormone-induced growth in lettuce and cucumber hypocotyls and spinach veil suspension cultures
I. Potter (1994)
The role of endoxyloglucan transferase in the organization of plant cell walls.
K. Nishitani (1997)
Xyloglucan endotransglycosylases have a func
EJ owicz (2002)
Changes in xyloglucan endotransglycosy - lase ( XET ) activity during hormone - induced growth in lettuce and cucumber hypocotyls and spinach cell suspension cultures
I Potter (1994)
C Barrachina (1998)
Antibodies: A Laboratory Manual
E. Harlow (1988)
An analysis of relative elemental
SJ Palmer (1996)
Density-labelling of cell wall polysaccharides in cultured rose cells: comparison of incorporation of 2H and 13C from exogenous glucose.
J. Thompson (2001)
Arabidopsis TCH4, regulated by hormones
Autolysis of the cell wall. Its possible role in endogenous and IAA-induced growth in epicotyls of Cicer arietinum
J. Seara (1988)
Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants.
S. Fry (1992)
Xyloglucan Endotransglycosylases Have a Function during the Formation of Secondary Cell Walls of Vascular Tissues Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.007773.
V. Bourquin (2002)
3H and 13C from exogenous glucose
JE Thompson (1998)
Xyloglucan Endotransglycosylase Activity Increases during Kiwifruit (Actinidia deliciosa) Ripening (Implications for Fruit Softening)
R. Redgwell (1993)
Expression of endoxyloglucan transferase genes in acaulis mutants of Arabidopsis.
T. Akamatsu (1999)
A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis.
R. Yokoyama (2001)
Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin.
T. Nakamura (2003)
The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature.
J. Rose (2002)
Light Control of Arabidopsis Development Entails Coordinated Regulation of Genome Expression and Cellular Pathways
L. Ma (2001)
Autolysis of the cell wall
J Seara (1988)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Isolation and analysis of genes from phytopathogenic fungi
T. Motoyama (2002)
Polysaccharide-Modifying Enzymes in the Plant Cell Wall
S. Fry (1995)
Cellular Localization of Arabidopsis Xyloglucan Endotransglycosylase-Related Proteins during Development and after Wind Stimulation
D. Antosiewicz (1997)
Differential expression of AtXTH17, -18, -19 and -20 genes in Arabidopsis roots
K. Vissenberg (2005)
The regulation of leaf elongation and xyloglucan endotransglycosylase by gibberellin in ‘Himalaya’ barley (Hordeum vulgare L.)
R. C. Smith (1996)
Dynamic changes in cell surface molecules are very early events in the differentiation of mesophyll cells from Zinnia elegans into tracheary elements
N. Stacey (1995)
Isolation and analyses of genes preferentially expressed during early cotton fiber development by subtractive PCR and cDNA array.
Sheng-Jian Ji (2003)
In vivo co-localization of xyloglucan endotrans
SC Fry (2000)
Expression of a xyloglucan endo
M Ishimaru (2002)
AtXTH27 plays an essential
Y Komeda (2005)
Differential expression of AtXTH17, -18
K. Nishitani (2004)
Xyloglucan endotransglycosylase, a new
KJ Matthews (1992)
RJ Redgwell (1993)
Differential expression of AtXTH17, AtXTH18, AtXTH19 and AtXTH20 genes in Arabidopsis roots. Physiological roles in specification in cell wall construction.
K. Vissenberg (2005)
Xyloglucan undergoes interpolymeric transglycosylation during binding to the plant cell wall in vivo: evidence from 13C/3H dual labelling and isopycnic centrifugation in caesium trifluoroacetate.
J. Thompson (1997)
Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella
U. Ryser (2002)
The gene for a xyloglucan endotransglucosylase/hydrolase from is strongly expressed in elongating tissues
S. Romo (2005)
AtXTH27 plays an essential role in cell wall modification during the development of tracheary elements.
A. Matsui (2005)
Xyloglucan endotransglucosylase hydrolase related to the elongation process 3987
Partial purification of cell wall β-galactosidases from Cicer arietinum epicotyls. Relationship with cell wall autolytic processes
B. Dopico (1989)
Soybean BRU1 Encodes a Functional Xyloglucan Endotransglycosylase That is Highly Expressed in Inner Epicotyl Tissues during Brassinosteroid-Promoted Elongation
M. Oh (1998)
In Vivo Colocalization of Xyloglucan Endotransglycosylase Activity and Its Donor Substrate in the Elongation Zone of Arabidopsis Roots
K. Vissenberg (2000)
L. Dillon (1987)
Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase.
W. Xu (1995)
The Arabidopsis XET-related gene family: environmental and hormonal regulation of expression.
W. Xu (1996)
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Abscisic acid delays chickpea germination by inhibiting water uptake and down-regulating genes encoding cell wall remodelling proteins
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Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabidopsis thaliana.
An Maris (2011)
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N. Nishikubo (2010)
Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion
Chien-Yuan Lin (2016)
βIII-Gal is involved in galactan reduction during phloem element differentiation in chickpea stems.
I. Martín (2013)
Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
R. Chen (2018)
The βI-galactosidase of Cicer arietinum is located in thickened cell walls such as those of collenchyma, sclerenchyma and vascular tissue.
I. Martín (2011)
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Sunita Kushwah (2020)
Cell Walls and Golgi
K. Vaughn (2013)
Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods
Courtney P Leisner (2014)
Microarray analysis of developing flax hypocotyls identifies novel transcripts correlated with specific stages of phloem fibre differentiation.
Melissa J. Roach (2008)
Arabidopsis XTH4 and XTH9 contribute to wood cell expansion and secondary wall formation
Sunita Kushwah (2019)
Organ accumulation and subcellular location of Cicer arietinum ST1 protein.
L. Albornos (2014)
Getting ready for host invasion: elevated expression and action of xyloglucan endotransglucosylases/hydrolases in developing haustoria of the holoparasitic angiosperm Cuscuta
S. Olsen (2016)