Online citations, reference lists, and bibliographies.
← Back to Search

Influence Of The Sequence-dependent Flexure Of DNA On Transcription In E. Coli.

C. M. Collis, P. Molloy, G. W. Both, H. Drew
Published 1989 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or curved) near the sites of cleavage by commonly-used restriction enzymes. One can use these plasmids to place any DNA region of interest (e.g., promoter, operator or enhancer) close to certain kinds of DNA structure that may influence its ability to work in a living cell. In the present example, we have placed a promoter from T7 virus next to the special DNA structures; the T7 promoter is then linked to a gene for a marker protein (chloramphenicol acetyl transferase). When plasmids bearing the T7 promoter are grown in cells of E. coli that contain T7 RNA polymerase, the special DNA structures seem to have little or no influence over the activity of the T7 promoter, contrary to our expectations. Yet when the same plasmids are grown in cells of E. coli that do not contain T7 RNA polymerase, some of the DNA structures show a surprising promoter activity of their own. In particular, the favourable flexibility or curvature of DNA, in the close vicinity of potential -35 and -10 promoter regions, seems to be a significant factor in determining where E. coli RNA polymerase starts RNA chains. We show directly, in one example, that loss of curvature between -35 and -10 regions is associated with a nearly-complete loss of promoter activity. These results, and others of their kind, show that the structural and/or vibrational properties of DNA play a much more important role in determining E. coli promoter activity than has previously been supposed.
This paper references

This paper is referenced by
Chromosome Biology
R. Appels (1998)
DNA bending and kinking — sequence dependence and function
A. Travers (1991)
A 3D pattern matching algorithm for DNA sequences
J. Hérisson (2007)
A positive cis-acting DNA element is required for high-level transcription in Chlamydia.
C. Schaumburg (2000)
RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions
Christine L Miller (2016)
Local supercoil-stabilized DNA structures.
E. Paleček (1991)
DNA Structure and Function
R. Sinden (1994)
Curved DNA and Prokaryotic Promoters
M. Asayama (2005)
Chapter 6 – DNA Supercoiling
C. R. Calladine (2004)
Interaction of nuclear proteins with intrinsically curved DNA in a matrix attachment region of a tobacco gene
Y. Fukuda (2004)
Variable Structure and Folding of DNA
R. Appels (1998)
Effects of DNA topology in the interaction with histone octamers and DNA topoisomerase I.
R. Negri (1994)
Conservation and Periodicity of DNA Bend Sites in the Human β-Globin Gene Locus (*)
Y. Wada-Kiyama (1995)
Regulació de l'expressió gènica a enterobactèries: caracterització d'YdgT, una nova proteïna de la família Hha/YmoA, i interacciona amb altres proteïnes associades al nucleoide
Sònia Paytubi Casabona (2004)
Fluorescence Assay for Polymerase Arrival Rates
A. Che (2003)
Role of the spacer between the −35 and −10 regions in σs promoter selectivity in Escherichia coli
Athanasios Typas (2006)
Different flexibility of the upstream regulatory regions of two differently expressed pea rbcS genes studied by theoretical evaluation of DNA distortion energy and cyclization kinetics
S. Cacchione (1993)
cAMP-CRP activator complex and the CytR repressor protein bind co-operatively to the cytRP promoter in Escherichia coli and CytR antagonizes the cAMP-CRP-induced DNA bend.
H. Pedersen (1992)
Nitrosamine-induced cancer: O4-alkylthymine produces sites of DNA hyperflexibility.
P. Georgiadis (1991)
Factor-independent activation of Escherichia coli rRNA transcription. I. Kinetic analysis of the roles of the upstream activator region and supercoiling on transcription of the rrnB P1 promoter in vitro.
S. Leirmo (1991)
Regulation of the tissue factor gene in human monocytic cells. Role of AP-1, NF-kappa B/Rel, and Sp1 proteins in uninduced and lipopolysaccharide-induced expression.
P. Oeth (1997)
The activator/repressor protein DnrO of Streptomyces peucetius binds to DNA without changing its topology.
Vasanthakumar Ajithkumar (2010)
Why bend DNA?
A. Travers (1990)
The expression and transcriptional organisation of region 1 of the Escherichia coli K5 capsule gene cluster
D. Simpson (1996)
In vitro transcription of a poly(dA) x poly(dT)-containing sequence is inhibited by interaction between the template and its transcripts.
R. Kiyama (1996)
Molecular genetic analysis of an FNR‐dependent anaerobically inducible Escherichia coli promoter
A. Bell (1990)
Chlamydia Required for High-Level Transcription in-Acting DNA Element Is cis A Positive
C. Schaumburg (2000)
Conservation of DNA curvature signals in regulatory regions of prokaryotic genes.
R. Jáuregui (2003)
Electrotransformation of Lactobacillus plantarum using linearized plasmid DNA
J. K. Thompson (1997)
T7 RNA polymerase cannot transcribe through a highly knotted DNA template.
J. Portugal (1996)
The complex architecture of mycobacterial promoters.
M. Newton-Foot (2013)
Synthetic DNA bending sequences increase the rate of in vitro transcription initiation at the Escherichia coli lac promoter.
M. Gartenberg (1991)
See more
Semantic Scholar Logo Some data provided by SemanticScholar