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Mitochondrial DNA Polymerase Gamma Is Expressed And Translated In The Absence Of Mitochondrial DNA Maintenance And Replication.

A. Davis, P. A. Ropp, D. Clayton, W. Copeland
Published 1996 · Biology, Medicine

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Mitochondria are essential organelles in all eukaryotic cells where cellular ATP is generated through the process of oxidative phosphorylation. Protein components of the respiratory assembly are gene products of both mitochondrial and nuclear genes. The mitochondrial genome itself encodes several protein and nucleic acid components required for such oxidative phosphorylative processes, but the vast majority of genes encoding respiratory chain components are nuclear. Similarly, the processes of replication and transcription of mitochondrial DNA rely exclusively upon RNA and protein species encoded by nuclear genes. We have analyzed two key nuclear-encoded proteins involved in mitochondrial DNA replication and transcription as a function of the presence or absence of mitochondrial DNA. Mitochondrial DNA polymerase (DNA polymerase gamma), the nuclear-encoded enzyme which synthesizes mtDNA, is expressed and translated in cells devoid of mitochondrial DNA itself. In contrast, mitochondrial transcription factor A protein levels are tightly linked to the mtDNA status of the cell. These results demonstrate that the DNA polymerase gamma protein is stable in the absence of mitochondrial DNA, and that there appears to be no regulatory mechanism present in these cells to alter levels of this protein in the complete absence of mitochondrial DNA. Alternatively, it is possible that this enzyme plays an additional, as yet undefined, role in the cell, thereby mandating its continued production.
This paper references
10.1128/MCB.15.12.7032
Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA.
I. Antoshechkin (1995)
10.1126/SCIENCE.2035027
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
M. Parisi (1991)
10.1111/J.1432-1033.1977.TB11946.X
Identity of DNA polymerase gamma from synaptosomal mitochondria and rat-brain nuclei.
U. Hübscher (1977)
10.1016/0022-2836(73)90100-9
5-bromodeoxyuridine labeling of monomeric and catenated circular mitochondrial DNA in HeLa cells.
P. J. Flory (1973)
10.1128/MCB.7.12.4431
Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product.
S. Wang (1987)
10.1016/0014-4827(72)90583-6
Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells.
M. M. Nass (1972)
10.1126/SCIENCE.3027892
The mitochondrial genotype can influence nuclear gene expression in yeast.
V. Parikh (1987)
DNA-repair reactions by purified HeLa DNA polymerases and exonucleases.
H. Randahl (1988)
10.1016/0012-1606(80)90474-1
DNA replication by isolated rat trophoblast nuclei. Characterization of the system and the product.
G. Kalf (1980)
10.1126/SCIENCE.2814477
Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation.
M. P. King (1989)
Cloning and sequencing of the nuclear gene MIP1 encoding the catalytic subunit of the yeast mitochondrial DNA polymerase.
F. Foury (1989)
10.1016/0092-8674(77)90286-0
Mouse L cell mitochondrial DNA molecules are selected randomly for replication throughout the cell cycle
D. Bogenhagen (1977)
10.1111/J.1432-1033.1984.TB08249.X
On auxotrophy for pyrimidines of respiration-deficient chick embryo cells.
M. Grégoire (1984)
10.1101/GAD.7.12A.2431
NRF-1, an activator involved in nuclear-mitochondrial interactions, utilizes a new DNA-binding domain conserved in a family of developmental regulators.
C. A. Virbasius (1993)
10.1006/BBRC.1994.1603
Low levels of mitochondrial transcription factor A in mitochondrial DNA depletion.
N. Larsson (1994)
10.1128/MCB.11.1.38
Intramitochondrial functions regulate nonmitochondrial citrate synthase (CIT2) expression in Saccharomyces cerevisiae.
X. Liao (1991)
Mitochondrial transcription initiation. Variation and conservation.
G. Shadel (1993)
10.1128/MCB.5.5.1163
Ethidium bromide-induced loss of mitochondrial DNA from primary chicken embryo fibroblasts.
Paul R. E. Desjardins (1985)
10.1111/J.1432-1033.1977.TB11945.X
Chick-embryo DNA polymerase gamma. Identity of gamma-polymerases purified from nuclei and mitochondria.
U. Bertazzoni (1977)
10.1016/0753-3322(94)90134-1
Mitochondrial DNA alterations and genetic diseases: a review.
P. Lestienne (1994)
10.1016/0014-5793(77)80528-0
Transport of citrate in submitochondrial particles
G. Prezioso (1977)
Antibodies: A Laboratory Manual
E. Harlow (1988)
10.1016/0092-8674(93)90050-Z
RTG1 and RTG2: Two yeast genes required for a novel path of communication from mitochondria to the nucleus
X. Liao (1993)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1016/0005-2787(80)90174-4
Characterization of DNA polymerases in mature sperm of the sea urchin.
A. Habara (1980)
10.1146/ANNUREV.CB.07.110191.002321
Replication and transcription of vertebrate mitochondrial DNA.
D. Clayton (1991)
10.1007/BF00270321
Reversible tenfod reduction in mitochondrial DNA content of human cells treated with ethidium bromide
A. Wiseman (2004)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)



This paper is referenced by
10.1016/S0070-2153(06)77010-7
Nuclear transfer: preservation of a nuclear genome at the expense of its associated mtDNA genome(s).
E. Bowles (2007)
10.1146/ANNUREV.BIOCHEM.72.121801.161455
DNA Polymerase γ, The Mitochondrial Replicase1
L. S. Kaguni (2004)
10.1136/sti.77.3.158
Mitochondrial toxicity and HIV therapy
A. J. White (2001)
10.1089/088922202753747879
Lack of correlation between length variation in the DNA polymerase gamma gene CAG repeat and lactic acidosis or neuropathy during antiretroviral treatment.
Xianghong Chen (2002)
Developing safer HIV drugs : elucidating the mechanistic pathway of Tenofovir-induced cytotoxicity via mitochondrial dysfunction
C. Mellor (2014)
10.1016/S0300-9084(99)00223-0
Expression of mitochondrial genes and of the transcription factors involved in the biogenesis of mitochondria Tfam, NRF-1 and NRF-2, in rat liver, testis and brain.
H. Escrivá (1999)
10.1016/j.expneurol.2015.02.004
Selective striatal mtDNA depletion in end-stage Huntington's disease R6/2 mice
Tanja Hering (2015)
10.1016/j.biochi.2011.01.004
Mitochondrial DNA replication and OXPHOS gene transcription show varied responsiveness to Rieske protein knockdown in 143B cells.
Oksana Levanets (2011)
Variación genética del mtDNA: De la genética poblacional a la farmacogenómica
Aurora Gómez Durán (2012)
10.1007/978-3-662-07426-8_5
Mitochondrial Genetics of the Budding Yeast Saccharomyces cerevisiae
K. Wolf (2004)
10.1093/CARCIN/BGI001
A new role for the von Hippel-Lindau tumor suppressor protein: stimulation of mitochondrial oxidative phosphorylation complex biogenesis.
E. Hervouet (2005)
10.1007/978-3-642-59884-5_2
Organization and Expression of the Mitochondrial Genome
J. Véziers (1999)
in Yeast Mdj 1 p in Inheritance of Mitochondrial DNA Dual Role of the Mitochondrial Chaperone
Jaroslaw Marszalek Westermann (1999)
10.1023/A:1022681828846
Nuclear Control of Respiratory Chain Expression in Mammalian Cells
R. Scarpulla (1997)
10.1016/S0005-2728(98)00161-3
The mitochondrial genome: structure, transcription, translation and replication.
J. Taanman (1999)
10.1091/MBC.11.4.1471
Rearrangements of human mitochondrial DNA (mtDNA): new insights into the regulation of mtDNA copy number and gene expression.
Y. Tang (2000)
10.1158/0008-5472.CAN-05-4103
The p53 pathway promotes efficient mitochondrial DNA base excision repair in colorectal cancer cells.
D. Chen (2006)
10.1073/PNAS.95.21.12244
Identification of 5'-deoxyribose phosphate lyase activity in human DNA polymerase gamma and its role in mitochondrial base excision repair in vitro.
M. Longley (1998)
10.1016/J.TAAP.2012.03.009
The effect of ethidium bromide and chloramphenicol on mitochondrial biogenesis in primary human fibroblasts.
Li-Pin Kao (2012)
10.1016/S0378-1119(00)00107-4
New features of mitochondrial DNA replication system in yeast and man.
N. Lecrenier (2000)
10.1100/tsw.2003.09
DNA Polymerase Gamma in Mitochondrial DNA Replication and Repair
W. Copeland (2003)
Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapses
J. E. Johnson (2007)
10.1093/NAR/GKH863
The C-terminal alphaO helix of human Ogg1 is essential for 8-oxoguanine DNA glycosylase activity: the mitochondrial beta-Ogg1 lacks this domain and does not have glycosylase activity.
K. Hashiguchi (2004)
10.1093/clinchem/45.10.1725
Sensitive Assay for Mitochondrial DNA Polymerase γ
R. Naviaux (1999)
10.1002/AJMG.1379
Diseases caused by nuclear genes affecting mtDNA stability.
A. Suomalainen (2001)
Towards an understanding of the side effects of anti-HIV drugs using Caenorhabditis elegans
Reuben L Smith (2016)
10.1017/S0953756297004462
One stop mycology
Lilyann Novak Frazer (1995)
10.1111/j.1742-4658.2005.04913.x
Mitochondrial biogenesis in mtDNA‐depleted cells involves a Ca2+‐dependent pathway and a reduced mitochondrial protein import
L. Mercy (2005)
10.1007/s004280050285
Immunohistochemical detection of human mtDNA polymerase gamma and of human mitochondrial transcription factor A in cytochrome-c-oxidase-deficient oxyphil cells of hyperfunctional parathyroids
J. Mueller-Hoecker (1998)
10.3923/IJP.2006.152.162
An Overview of Mitochondrial Toxicity of Nucleoside Reverse Transcriptase Inhibitors Associated with HIV Therapy
.. C.N.Fokunang (2006)
10.1023/A:1006983412952
Mitochondrial involvement in bladder function and dysfunction
C. A. nevel-McGarvey (2004)
10.3390/ph5101103
Mitochondria-Targeted Antioxidant SS31 Prevents Amyloid Beta-Induced Mitochondrial Abnormalities and Synaptic Degeneration in Alzheimer’s Disease
M. Calkins (2012)
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