← Back to Search
AtATG Genes, Homologs Of Yeast Autophagy Genes, Are Involved In Constitutive Autophagy In Arabidopsis Root Tip Cells.
Y. Inoue, T. Suzuki, Masaki Hattori, K. Yoshimoto, Y. Ohsumi, Y. Moriyasu
Published 2006 · Biology, Medicine
Download PDFAnalyze on Scholarcy
In Arabidopsis root tips cultured in medium containing sufficient nutrients and the membrane-permeable protease inhibitor E-64d, parts of the cytoplasm accumulated in the vacuoles of the cells from the meristematic zone to the elongation zone. Also in barley root tips treated with E-64, parts of the cytoplasm accumulated in autolysosomes and pre-existing central vacuoles. These results suggest that vacuolar and/or lysosomal autophagy occurs constitutively in these regions of cells. 3-Methyladenine, an inhibitor of autophagy, inhibited the accumulation of such inclusions in Arabidopsis root tip cells. Such inclusions were also not observed in root tips prepared from Arabidopsis T-DNA mutants in which AtATG2 or AtATG5, an Arabidopsis homolog of yeast ATG genes essential for autophagy, is disrupted. In contrast, an atatg9 mutant, in which another homolog of ATG is disrupted, accumulated a significant number of vacuolar inclusions in the presence of E-64d. These results suggest that both AtAtg2 and AtAtg5 proteins are essential for autophagy whereas AtAtg9 protein contributes to, but is not essential for, autophagy in Arabidopsis root tip cells. Autophagy that is sensitive to 3-methyladenine and dependent on Atg proteins constitutively occurs in the root tip cells of Arabidopsis.
This paper references
The Atg1-Atg13 complex regulates Atg9 and Atg23 retrieval transport from the pre-autophagosomal structure.
F. Reggiori (2004)
Life After Death: Are Autophagy Genes Involved in Cell Death and Survival During Plant Innate Immune Responses?
Montrell D Seay (2005)
Cytochemical studies on GERL, provacuoles, and vacuoles in root meristematic cells of Euphorbia.
F. Marty (1978)
In vitro and in vivo inhibition of cysteine proteinases by EST, a new analog of E-64.
M. Tamai (1986)
Endoplasmic reticulum-resident proteins are constitutively transported to vacuoles for degradation.
K. Tamura (2004)
Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin.
E. Kominami (1983)
Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction
K. Takeshige (1992)
Leaf Senescence and Starvation-Induced Chlorosis Are Accelerated by the Disruption of an Arabidopsis Autophagy Gene1
Hideki Hanaoka (2002)
Autophagy is not a main contributor to the degradation of phospholipids in tobacco cells cultured under sucrose starvation conditions.
Y. Inoue (2006)
Ultrastructural and biochemical characterization of autophagy in higher plant cells subjected to carbon deprivation: control by the supply of mitochondria with respiratory substrates
S. Aubert (1996)
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
J. Alonso (2003)
Expression of alpha-amylases, carbohydrate metabolism, and autophagy in cultured rice cells is coordinately regulated by sugar nutrient.
M. Chen (1994)
Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae
M. Tsukada (1993)
Isolation of autophagocytosis mutants of Saccharomyces cerevisiae
M. Thumm (1994)
Life After Death
C. Woods (2002)
Autophagic Nutrient Recycling in Arabidopsis Directed by the ATG8 and ATG12 Conjugation Pathways1
Allison R Thompson (2005)
Atg9 Cycles Between Mitochondria and the Pre-Autophagosomal Structure in Yeasts
F. Reggiori (2005)
Autophagy Regulates Programmed Cell Death during the Plant Innate Immune Response
Y. Liu (2005)
Apg9p/Cvt7p Is an Integral Membrane Protein Required for Transport Vesicle Formation in the Cvt and Autophagy Pathways
Takeshi Noda (2000)
Zur Entwicklung der Vacuole in Testa-Zellen des Leinsamens
F. Amelunxen (1984)
Processing of ATG8s, Ubiquitin-Like Proteins, and Their Deconjugation by ATG4s Are Essential for Plant Autophagy
K. Yoshimoto (2004)
A High-Throughput Arabidopsis Reverse Genetics System Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.004630.
A. Sessions (2002)
6-substituted purines: a novel class of inhibitors of endogenous protein degradation in isolated rat hepatocytes.
P. B. Gordon (1982)
The pre‐autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation
K. Suzuki (2001)
Formation Process of Autophagosome Is Traced with Apg8/Aut7p in Yeast
T. Kirisako (1999)
On the development of the vacuole. II: Further evidence for endoplasmic reticulum origin
B. Hilling (1985)
A unified nomenclature for yeast autophagy-related genes.
D. Klionsky (2003)
The autophagy-associated Atg8 gene family operates both under favourable growth conditions and under starvation stresses in Arabidopsis plants.
S. Sláviková (2005)
Autophagy and related mechanisms of lysosome-mediated protein degradation.
W. Dunn (1994)
Vacuolar import of proteins and organelles from the cytoplasm.
D. Klionsky (1999)
Alpha tonoplast intrinsic protein is specifically associated with vacuole membrane involved in an autophagic process.
Y. Moriyasu (2003)
The APG8/12-activating Enzyme APG7 Is Required for Proper Nutrient Recycling and Senescence in Arabidopsis thaliana *
J. H. Doelling (2002)
3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes.
P. Seglen (1982)
3-methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions.
Chihiro Takatsuka (2004)
Autophagy in Tobacco Suspension-Cultured Cells in Response to Sucrose Starvation
Y. Moriyasu (1996)
Examination of the Contribution of Vacuolar Proteases to Intracellular Protein Degradation in Chara corallina
Y. Moriyasu (1995)
This paper is referenced by
The ABA-regulated Arabidopsis TSPO is an ER/Golgi localized heme binding membrane protein and a potential scavenger of porphyrins via an autophagy-dependent degradation mechanism
Celine Vanhee (2011)
Pollen Germination and Tube Growth
D.-Q. Shi (2010)
Functional analysis of glutathione and autophagy in response to oxidative stress
Yi Han (2012)
Microautophagy in Plants: Consideration of Its Molecular Mechanism
K. Sieńko (2020)
The Rab GTPase RabG3b functions in autophagy and contributes to tracheary element differentiation in Arabidopsis.
S. Kwon (2010)
Nutrient starvation differentially regulates the autophagy-related gene GmATG8i in soybean seedlings
Nang Myint Phyu Sin Htwe (2009)
From signal transduction to autophagy of plant cell organelles: lessons from yeast and mammals and plant-specific features
S. Reumann (2010)
Activation of autophagy by unfolded proteins during endoplasmic reticulum stress.
Xiaochen Yang (2016)
Autophagy Negatively Regulates Cell Death by Controlling NPR1-Dependent Salicylic Acid Signaling during Senescence and the Innate Immune Response in Arabidopsis[W][OA]
K. Yoshimoto (2009)
Autophagic proteins ATG4 and ATG8 in wheat: Structural characteristics and their role under stress conditions
V. Ryabovol (2014)
Molecular analysis of glyphosate and osmotic stress responsive genes
Özge Cebeci Yalçınkaya (2010)
Evidence for autophagy-dependent pathways of rRNA turnover in Arabidopsis
Brice E. Floyd (2015)
The ATG1/ATG13 Protein Kinase Complex Is Both a Regulator and a Target of Autophagic Recycling in Arabidopsis[C][W]
Anongpat Suttangkakul (2011)
Birth, Growth, Maturation, and Demise of Plant Autophagic Vesicles
J. H. Kim (2020)
Autophagy-Like Cell Death Regulates Hydrogen Peroxide and Calcium Ion Distribution in Xa3/Xa26-Mediated Resistance to Xanthomonas oryzae pv. oryzae
Jianbo Cao (2019)
The Root Hair Assay Facilitates the Use of Genetic and Pharmacological Tools in Order to Dissect Multiple Signalling Pathways That Lead to Programmed Cell Death
J. Kacprzyk (2014)
Sucrose Starvation Induces Microautophagy in Plant Root Cells
Shino Goto-Yamada (2019)
Highly Oxidized Peroxisomes Are Selectively Degraded via Autophagy in Arabidopsis[C][W]
Michitaro Shibata (2013)
Analysis of Plant Autophagy.
Liang Chen (2017)
Constitutive Autophagy in Plant Root Cells
Kanako Yano (2007)
Homologs of genes associated with programmed cell death in animal cells are differentially expressed during senescence of Ipomoea nil petals.
Tetsuya Yamada (2009)
Pumping up the volume - vacuole biogenesis in Arabidopsis thaliana.
Falco Krüger (2018)
Autophagy is required for tolerance of drought and salt stress in plants
Yimo Liu (2009)
Guidelines for the use and interpretation of assays for monitoring autophagy
D. Klionsky (2012)
The roles of autophagy in development and stress responses in Arabidopsis thaliana
X. Lv (2014)
Investigating the role of papain-like cysteine protease RD21 in plant-pathogen interactions
T. Shindo (2010)
Analysis of the function and the regulation of autophagy in Arabidopsis thaliana
Yimo Liu (2012)
Autophagy-mediated Nutrient Recycling and Regulation in Plants: A Molecular View
Mousona Islam (2019)
Degradation of plant peroxisomes by autophagy
Han Nim Lee (2014)
Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation[C][W]
L. Farmer (2013)
The Secretory System of Arabidopsis
D. Bassham (2008)
Protein degradation and peptidase activity during petal senescence in Dendrobium cv. Khao Sanan
L. Lerslerwong (2009)See more