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Direct Interaction Of Resistance Gene And Avirulence Gene Products Confers Rice Blast Resistance

Y. Jia, S. McAdams, G. Bryan, H. Hershey, B. Valent
Published 2000 · Medicine, Biology

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Rice expressing the Pi‐ta gene is resistant to strains of the rice blast fungus, Magnaporthe grisea, expressing AVR‐Pita in a gene‐for‐gene relationship. Pi‐ta encodes a putative cytoplasmic receptor with a centrally localized nucleotide‐binding site and leucine‐rich domain (LRD) at the C‐terminus. AVR‐Pita is predicted to encode a metalloprotease with an N‐terminal secretory signal and pro‐protein sequences. AVR‐Pita176 lacks the secretory and pro‐protein sequences. We report here that transient expression of AVR‐Pita176 inside plant cells results in a Pi‐ta‐dependent resistance response. AVR‐Pita176 protein is shown to bind specifically to the LRD of the Pi‐ta protein, both in the yeast two‐hybrid system and in an in vitro binding assay. Single amino acid substitutions in the Pi‐ta LRD or in the AVR‐Pita176 protease motif that result in loss of resistance in the plant also disrupt the physical interaction, both in yeast and in vitro. These data suggest that the AVR‐Pita176 protein binds directly to the Pi‐ta LRD region inside the plant cell to initiate a Pi‐ta‐mediated defense response.
This paper references
Structure of the Arabidopsis RPM1 gene enabling dual specificity disease resistance
M. Grant (1995)
Note added in proof
Jna (1984)
Fungal avirulence genes and plant resistance genes: unravelling the molecular basis of gene-for-gene interactions.
P. D. Wit (1995)
Active Oxygen Species in Plant Defense against Pathogens
M. Mehdy (1994)
Identification, cloning, and characterization of PWL2, a gene for host species specificity in the rice blast fungus.
J. Sweigard (1995)
Correlation between binding affinity and necrosis-inducing activity of mutant AVR9 peptide elicitors.
M. Kooman-Gersmann (1998)
Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death.
S. Gopalan (1996)
A resistance gene product of the nucleotide binding site -- leucine rich repeats class can form a complex with bacterial avirulence proteins in vivo.
R. Leister (2000)
The hypersensitive reaction in plants to pathogens: a resistance phenomenon.
R. Goodman (1994)
The Tomato Cf-2 Disease Resistance Locus Comprises Two Functional Genes Encoding Leucine-Rich Repeat Proteins
M. Dixon (1996)
A Telomeric Avirulence Gene Determines Efficacy for the Rice Blast Resistance Gene Pi-ta
M. Orbach (2000)
Tobacco mosaic virus elicitor coat protein genes produce a hypersensitive phenotype in transgenic Nicotiana sylvestris plants
J. Culver (1991)
The enigmatic avirulence genes of phytopathogenic bacteria.
J. Dangl (1994)
Transient expression of b-glucuronidase in Arabidopsis thaliana leaves and Brassica napus stems using a pneumatic particle gun
M Seki (1991)
Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats
B. Kobe (1993)
Fungal Infection of Plants.
Wolfgang Knogge (1996)
The Role of Leucine-Rich Repeat Proteins in Plant Defences
David A Jones (1997)
The roles of leucine rich repeats in plant defences
D A Jones (1996)
Constitutively active Pto induces a Prf‐dependent hypersensitive response in the absence of avrPto
J. Rathjen (1999)
Xa21D Encodes a Receptor-like Molecule with a Leucine-Rich Repeat Domain That Determines Race-Specific Recognition and Is Subject to Adaptive Evolution
G. G. Wang (1998)
Molecular genetic analysis of the rice blast fungus, magnaporthe grisea.
B. Valent (1991)
Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses.
B. Valent (1991)
Plant Disease Resistance Genes: Function Meets Structure.
A. Bent (1996)
Type III secretion machines: bacterial devices for protein delivery into host cells.
J. Galán (1999)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
A transcriptional co-repressor that interacts with nuclear hormone receptors
J. Chen (1995)
Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato
S. Scofield (1996)
The race‐specific elicitor, NIP1, from the barley pathogen, Rhynchosporium secalis, determines avirulence on host plants of the Rrs1 resistance genotype.
M. Rohe (1995)
Plant disease resistant genes
K. Hammond-Kosack (1997)
Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto Kinase
Xiaoyan Tang (1996)
Type III secretion machines: bacterial device for protein delivery into host cells
J E Galan (1994)
Early events in the activation of plant defense responses
R. Dixon (1994)
A High-Affinity Binding Site for the AVR9 Peptide Elicitor of Cladosporium fulvum Is Present on Plasma Membranes of Tomato and Other Solanaceous Plants.
M. Kooman-Gersmann (1996)
A novel genetic system to detect protein–protein interactions
S. Fields (1989)
Penetration of hard substrates by a fungus employing enormous turgor pressures.
R. Howard (1991)
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)
Map-based cloning of a protein kinase gene conferring disease resistance in tomato.
G. Martin (1993)
Molecular recognition of pathogen attack occurs inside of plant cells in plant disease resistance specified by the Arabidopsis genes RPS2 and RPM1.
R. Leister (1996)
Recognition of the Bacterial Avirulence Protein AvrBs3 Occurs inside the Host Plant Cell
G. V. D. Ackerveken (1996)
K. Hammond-Kosack (1997)
Recognition specificity for the bacterial avirulence protein AvrPto is determined by Thr-204 in the activation loop of the tomato Pto kinase.
R. Frederick (1998)
Identi®cation, cloning, and characterization of PWL2, a gene for host species speci®city in the rice blast fungus
J A Sweigard (1995)
Host resistance to a fungal tomato pathogen lost by a single base-pair change in an avirulence gene
M. Joosten (1994)
Amino‐terminal leucine‐rich repeats in gonadotropin receptors determine hormone selectivity.
T. Braun (1991)
The A. thaliana disease resistance gene RPS2 encodes a protein containing a nucleotide-binding site and leucine-rich repeats.
M. Mindrinos (1994)
The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids
P. Vos (1998)
Transient expression of β-glucuronidase in Arabidopsis thaliana leaves and roots and Brassica napus stems using a pneumatic particle gun
M. Seki (2004)
A Single Amino Acid Difference Distinguishes Resistant and Susceptible Alleles of the Rice Blast Resistance Gene Pi-ta
G. Bryan (2000)
The Rice Blast Fungus, Magnaporthe grisea
B. Valent (1997)
A revised medium for rapid growth Signal recognition in plant disease resistance and bioassays with tobacco tissue cultures
T Murashige (1962)
Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem
D. Silué (1992)
Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region.
P. A. Anderson (1997)
Alleles of Pto and Fen occur in bacterial speck-susceptible and fenthion-insensitive tomato cultivars and encode active protein kinases.
Y. Jia (1997)
The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response.
D. C. Boyes (1998)
Current Status of the Gene-For-Gene Concept
H. H. Flor (1971)
Tomato Prf Is a Member of the Leucine-Rich Repeat Class of Plant Disease Resistance Genes and Lies Embedded within the Pto Kinase Gene Cluster
J. Salmeron (1996)
Recognition of bacterial avirulence proteins occurs inside the plant cell: a general phenomenon in resistance to bacterial diseases?
U. Bonas (1997)
Maize Adh1.
M. Freeling (1985)
Alleles of Pto and Fen occur in bacterial speck-susceptible and fenthion-insensitive tomato lines and encode functional protein kinases
Y Jia (1994)
Elucidation of the thermal stability of the neutral proteinase II from Aspergillus oryzae.
H. Tatsumi (1994)

This paper is referenced by
Genetic and molecular analysis of resistance protein mediated plant immunity
X. Fang (2014)
Involvement of cathepsin B-like genes in disease resistance in Arabidopsis thaliana
H. McLellan (2008)
Plant Immunity
J. McDowell (2011)
Functional Dissection of a Rice Dr1/DrAp1 Transcriptional Repression Complex Article, publication date, and citation information can be found at
W. Song (2002)
Dual disease resistance mediated by the immune receptor Cf-2 in tomato requires a common virulence target of a fungus and a nematode
Jose L Lozano-Torres (2012)
Análise da variabilidade de Magnaporthe grisea no Estado de Santa Catarina
K. Scheuermann (2002)
A genetic map of Blumeria graminis based on functional genes, avirulence genes, and molecular markers.
C. Pedersen (2002)
Innate immunity in plants: a continuum of layered defenses.
Luis da Cunha (2006)
A Genome-Wide Survey of R Gene Polymorphisms in Arabidopsis[W]
E. Bakker (2006)
The Tomato NBARC-LRR Protein Prf Interacts with Pto Kinase in Vivo to Regulate Specific Plant Immunity[W]
Tatiana S. Mucyn (2006)
Visions & Reflections (Minireview) How plants recognize pathogens and defend themselves
P. D. Wit (2007)
RNA silencing is required for Arabidopsis defence against Verticillium wilt disease
U. Ellendorff (2009)
Endosome-Associated CRT1 Functions Early in Resistance Gene–Mediated Defense Signaling in Arabidopsis and Tobacco[W]
H. Kang (2010)
Two Distinct Pseudomonas Effector Proteins Interact with the Pto Kinase and Activate Plant Immunity
Y. Kim (2002)
Putative protease inhibitor gene discovery and transcript profiling during fruit development and leaf damage in grapefruit (Citrus paradisi Macf.).
R. Shatters (2004)
Functional and Structural Analyses of RTP1, a Rust Haustorial Protein Transferred into Host Plant Cells
Rafiqi Maryam (2007)
Development and characterization of blast resistance using differential varieties in rice
Y. Fukuta (2009)
Investigation of the recognition and host target of the Phytophthora infestans effector PiAVR2
S. Breen (2012)
Transcription Factors that Regulate Defence Responses and Their Use in Increasing Disease Resistance
Prateek Tripathi (2016)
Comparative secretome analysis of Colletotrichum falcatum identifies a cerato-platanin protein (EPL1) as a potential pathogen-associated molecular pattern (PAMP) inducing systemic resistance in sugarcane.
N. M. R. Ashwin (2017)
Dissecting R gene and host genetic background effect on the Brassica napus defense response to Leptosphaeria maculans
P. Haddadi (2019)
Nucleocytoplasmic Distribution Is Required for Activation of Resistance by the Potato NB-LRR Receptor Rx1 and Is Balanced by Its Functional Domains[W]
E. Slootweg (2010)
RanGAP2 Mediates Nucleocytoplasmic Partitioning of the NB-LRR Immune Receptor Rx in the Solanaceae, Thereby Dictating Rx Function[W][OA]
W. Tameling (2010)
Reviews Under Pressure : Investigating the Biology of Plant Infection by Magnaporthe oryza
R. A. Wilson (2013)
SIVB 2003 Congress Symposium Proceeding: Plant-Targets of Pathogenic Effectors Can Transduce Both Virulence and Resistance Signals
D. Mackey (2004)
Differential Recognition of Highly Divergent Downy Mildew Avirulence Gene Alleles by RPP1 Resistance Genes from Two Arabidopsis Lines
A. Rehmany (2005)
Characterization of a novel bacterial PAMP - elongation factor Tu - and its role in "Arabidopsis thaliana" defense and immunity
G. Kunze (2005)
Mode d'action de l'acide ß-aminobutirique chez la vigne : un inducteur de résistance aux pathogènes et étude des mécanismes impliqués dans la sensibilité aux pathogènes du mutant PAD2 d'arabidopsis déficient en glutathion
C. Dubreuil-Maurizi (2010)
Unequal recombination and other rearrangements in plant nuclear and chloroplast genomes
Daniel C. Frailey (2017)
The analogous and opposing roles of double-stranded RNA-binding proteins in bacterial resistance
Gah-Hyun Lim (2019)
Engineering Disease Resistance in rice
Subhankar Roy-Barman (2013)
DNA polymorphism in the blast disease resistance gene Pita of the wild rice Oryza rufipogon and its related species.
K. Yoshida (2009)
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