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Fibrous Shape Underlies The Mutagenic And Carcinogenic Potential Of Nanosilver While Surface Chemistry Affects The Biosafety Of Iron Oxide Nanoparticles

A. Gábelová, N. el Yamani, Tamara Iglesias Alonso, Barbora Buliaková, A. Srancikova, A. Bábelová, Elise Runden Pran, Lise Marie Fjellsbø, Elisabeth Elje, Mazyar Yazdani, Maria João Silva, M. Dusinska
Published 2017 · Chemistry, Medicine

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Nowadays engineered nanomaterials (ENMs) are increasingly used in a wide range of commercial products and biomedical applications. Despite this, the knowledge of human potential health risk as well as comprehensive biological and toxicological information is still limited. We have investigated the capacity of two frequently used metallic ENMs, nanosilver and magnetite nanoparticles (MNPs), to induce thymidine kinase (Tk +/−) mutations in L5178Y mouse lymphoma cells and transformed foci in Bhas 42 cells. Two types of nanosilver, spherical nanoparticles (AgNM300) and fibrous (AgNM302) nanorods/wires, and MNPs differing in surface modifications [MNPs coated with sodium oleate (SO-MNPs), MNPs coated with SO + polyethylene glycol (SO-PEG-MNPs) and MNPs coated with SO + PEG + poly(lactide-co-glycolic acid) SO-PEG-PLGA-MNPs] were included in this study. Spherical AgNM300 showed neither mutagenic nor carcinogenic potential. In contrast, silver nanorods/wires (AgNM302) increased significantly the number of both gene mutations and transformed foci compared with the control (untreated) cells. Under the same treatment conditions, neither SO-MNPs nor SO-PEG-PLGA-MNPs increased the mutant frequency compared with control cells though an equivocal mutagenic effect was estimated for SO-PEG-MNPs. Although SO-MNPs and SO-PEG-MNPs did not show any carcinogenic potential, SO-PEG-PLGA-MNPs increased concentration dependently the number of transformed foci in Bhas 42 cells compared with the control cells. Our results revealed that fibrous shape underlies the mutagenic and carcinogenic potential of nanosilver while surface chemistry affects the biosafety of MNPs. Considering that both nanosilver and MNPs are prospective ENMs for biomedical applications, further toxicological evaluations are warranted to assess comprehensively the biosafety of these nanomaterials.
This paper references
Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release
A. Gliga (2013)
Evaluation of the mouse lymphoma tk assay (microwell method) as an alternative to the in vitro chromosomal aberration test.
M. Honma (1999)
Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis.
M. Piao (2011)
Genotoxicity testing of PLGA-PEO nanoparticles in TK6 cells by the comet assay and the cytokinesis-block micronucleus assay.
A. Kažimı́rová (2012)
Toxicology of Nanomaterials Used in Nanomedicine
J. Zhao (2011)
A Bhas 42 cell transformation assay on 98 chemicals: the characteristics and performance for the prediction of chemical carcinogenicity.
A. Sakai (2010)
Pulmonary toxicity of carbon nanotubes and asbestos - similarities and differences.
K. Donaldson (2013)
Genotoxicity of metal oxide nanomaterials: review of recent data and discussion of possible mechanisms.
Nazanin Golbamaki (2015)
Mouse lymphoma thymidine kinase gene mutation assay: Follow‐up meeting of the international workshop on Genotoxicity testing—Aberdeen, Scotland, 2003—Assay acceptance criteria, positive controls, and data evaluation
M. Moore (2006)
Cell transformation assays for prediction of carcinogenic potential: state of the science and future research needs
S. Creton (2012)
Ultrasmall superparamagnetic particles of iron oxide allow for the detection of metastases in normal sized pelvic lymph nodes of patients with bladder and/or prostate cancer.
M. Triantafyllou (2013)
Effect of silver nanoparticles on mitogen-activated protein kinases activation: role of reactive oxygen species and implication in DNA damage.
A. Rinna (2015)
The mouse lymphoma assay.
J. Clements (2000)
Nanosilver: a nanoproduct in medical application.
X. Chen (2008)
Microarray analysis distinguishes differential gene expression patterns from large and small colony Thymidine kinase mutants of L5178Y mouse lymphoma cells
Tao Han (2006)
Mutant frequencies and loss of heterozygosity induced by N-ethyl-N-nitrosourea in the thymidine kinase gene of L5178Y/TK(+/-)-3.7.2C mouse lymphoma cells.
T. Chen (2002)
Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating
A. Huk (2015)
Silver nanowire exposure results in internalization and toxicity to Daphnia magna.
Leona D. Scanlan (2013)
Superparamagnetic iron oxide nanoparticles as radiosensitizer via enhanced reactive oxygen species formation.
S. Klein (2012)
Encapsulation of anticancer drug and magnetic particles in biodegradable polymer nanospheres.
M. Koneracká (2008)
Review of the Evidence from Epidemiology, Toxicology, and Lung Bioavailability on the Carcinogenicity of Inhaled Iron Oxide Particulates.
C. Pease (2016)
Can the L5178Y Tk+/- mouse lymphoma assay detect epigenetic silencing?
Tsu-Fan Cheng (2013)
Encapsulation of indomethacin in magnetic biodegradable polymer nanoparticles
V. Závišová (2007)
Engineering nanosilver as an antibacterial, biosensor and bioimaging material.
G. Sotiriou (2011)
Analysis of large and small colony L5178Y tk-/- mouse lymphoma mutants by loss of heterozygosity (LOH) and by whole chromosome 11 painting: detection of recombination.
M. Liechty (1998)
Isolation and Characterization of ras‐Transfected BALB/3T3 Clone Showing Morphological Transformation by 12‐O‐Tetradecanoyl‐phorbol‐13‐acetate
K. Sasaki (1988)
Hydrolytic Degradation of Poly(D,L-lactide)(PDLLA)D,L-lactide/PDLLA-Poly(ethylene glycol)(PEG)-PDLLA Blend System
진인주 (2002)
Transformation Assay in Bhas 42 Cells: A Model Using Initiated Cells to Study Mechanisms of Carcinogenesis and Predict Carcinogenic Potential of Chemicals
K. Sasaki (2015)
Nanocapsule formation by interfacial polymer deposition following solvent displacement
H. Fessi (1989)
Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications.
Q. Li (2008)
Comparison of sensitivity to arsenic compounds between a Bhas 42 cell transformation assay and a BALB/c 3T3 cell transformation assay.
D. Muramatsu (2009)
Carcinogenic potential of metal nanoparticles in BALB/3T3 cell transformation assay
G. Sighinolfi (2016)
The role of reactive oxygen species in the genotoxicity of surface-modified magnetite nanoparticles.
M. Mesárošová (2014)
Molecular toxicity mechanism of nanosilver.
Danielle McShan (2014)
Spindle poisons induce allelic loss in mouse lymphoma cells through mitotic non-disjunction.
M. Honma (2001)
Biomaterials with Antibacterial and Osteoinductive Properties to Repair Infected Bone Defects
H. Lu (2016)
Application of hyperthermia induced by superparamagnetic iron oxide nanoparticles in glioma treatment
A. C. Silva (2011)
Efficient drug-delivery using magnetic nanoparticles--biodistribution and therapeutic effects in tumour bearing rabbits.
R. Tietze (2013)
Biomedical applications of distally controlled magnetic nanoparticles.
J. Corchero (2009)
Length-dependent pathogenic effects of nickel nanowires in the lungs and the peritoneal cavity
Craig A. Poland (2012)
The intensity of internalization and cytotoxicity of superparamagnetic iron oxide nanoparticles with different surface modifications in human tumor and diploid lung cells.
M. Mesárošová (2012)
Highly efficient magnetic stem cell labeling with citrate-coated superparamagnetic iron oxide nanoparticles for MRI tracking.
K. Andreas (2012)
In vitro evaluation of the genotoxicity of a family of novel MeO-PEGpoly(D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA
L. He (2009)
Short versus long silver nanowires: a comparison of in vivo pulmonary effects post instillation
R. Silva (2014)
In vitro evaluation of the genotoxicity of a family of novel MeO-PEG-poly(D,L-lactic-co-glycolic acid)-PEG-OMe triblock copolymer and PLGA nanoparticles.
L. He (2009)
Nanotoxicology and in vitro studies: the need of the hour.
Sumit Arora (2012)
A review on the application of inorganic nano-structured materials in the modification of textiles: focus on anti-microbial properties.
R. Dastjerdi (2010)
Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity
Vanessa Valdiglesias (2015)
Toxic effects of silver nanoparticles in mammals--does a risk of neurotoxicity exist?
Joanna Skalska (2015)
Nanosilver as a new generation of nanoproduct in biomedical applications.
K. Chaloupka (2010)
The mouse lymphoma assay detects recombination, deletion, and aneuploidy.
Jianyong Wang (2009)
Silver nanoparticles induce premutagenic DNA oxidation that can be prevented by phytochemicals from Gentiana asclepiadea.
Alexandra Hudecová (2012)
The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells
C. Siciliano (2015)
Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity.
N. Duran (2016)
Toxic effects of silver nanoparticles and nanowires on erythrocyte rheology.
M. Kim (2014)
Nanocrystalline Silver: A Systematic Review of Randomized Trials Conducted on Burned Patients and an Evidence-Based Assessment of Potential Advantages Over Older Silver Formulations
G. Gravante (2009)
Long-circulating and target-specific nanoparticles: theory to practice.
S. Moghimi (2001)
Aquatic Toxicity Comparison of Silver Nanoparticles and Silver Nanowires
Eun Kyung Sohn (2015)
Establishment of a highly reproducible transformation assay of a ras-transfected BALB 3T3 clone by treatment with promoters.
K. Sasaki (1990)
Silver nanoparticle‐induced mutations and oxidative stress in mouse lymphoma cells
N. Mei (2012)
An international validation study of a Bhas 42 cell transformation assay for the prediction of chemical carcinogenicity.
A. Sakai (2011)
Is the toxic potential of nanosilver dependent on its size?
A. Huk (2014)
Autophagy induction by silver nanowires: a new aspect in the biocompatibility assessment of nanocomposite thin films.
N. Verma (2012)
NM-300 Silver Characterisation, Stability, Homogeneity
C. Sara (2011)
Surface-mediated production of hydroxyl radicals as a mechanism of iron oxide nanoparticle biotoxicity.
M. Voinov (2011)
Bhas42 cell transformation assay as a predictor of carcinogenicity
A. Poth (2008)
Use of silver nanowires to determine thresholds for fibre length-dependent pulmonary inflammation and inhibition of macrophage migration in vitro
Anja Schinwald (2012)
Particle length-dependent titanium dioxide nanomaterials toxicity and bioactivity
R. Hamilton (2009)
2016) Silver nanoparticles: a new view on mechanistic aspects on antimicrobial
N. Duran (2016)
Magnetic fluid poly(ethylene glycol) with moderate anticancer activity
V. Závišová (2011)
Rhamnose‐coated superparamagnetic iron‐oxide nanoparticles: an evaluation of their in vitro cytotoxicity, genotoxicity and carcinogenicity
Alessandro Paolini (2016)

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