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Isolation And Direct Complete Nucleotide Determination Of Entire Genes. Characterization Of A Gene Coding For 16S Ribosomal RNA.

U Edwards, Till Rogall, Helmut Blöcker, Markus Emde, Erik C Böttger
Published 1989 · Medicine, Biology
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Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA. As a model system the nucleotide sequence of the 16S rRNA gene of M.kansasii was determined and found to be 98.7% homologous to that of M.bovis BCG. This is the first report on a contiguous sequence information of an entire amplified gene spanning 1.5 kb without any subcloning procedures.



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10.1128/AEM.00913-08
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M Julien (2008)
10.1016/j.fm.2016.03.002
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10.1264/jsme2.ME19030
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10.28930/jitkt.v3i1.7838
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10.1590/S0100-204X2008000600004
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10.1007/s10658-020-02010-z
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Ana M. Fernández-Sanz (2020)
10.1099/ijsem.0.003753
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Dana Nováková (2019)
10.1099/ijsem.0.003026
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Jakko van Ingen (2018)
10.1007/BF02756750
Phylogenetic position of desulfurococcus amylolyticus
Tatjana P. Tourova (2007)
10.1016/j.femsec.2004.02.001
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Deborah T. Newby (2004)
Efficiency of some soil bacteria for chemical oxygen demand reduction of synthetic chlorsulfuron solutions under agiated culture conditions.
Gokhan Onder Erguven (2016)
10.1007/s00284-006-0136-0
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10.1590/0001-3765201920180394
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M S Hughes (1993)
10.1002/ece3.4595
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10.1139/w05-109
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10.1016/J.RHISPH.2017.01.001
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10.1099/ijsem.0.001723
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Shih-Yao Lin (2017)
10.1080/09583150701818972
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10.1016/j.ijpara.2009.01.006
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Annelies Haegeman (2009)
10.7717/peerj.6169
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Beatriz Roncero-Ramos (2019)
10.1016/j.intimp.2020.106191
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10.4454/JPP.V96I3.019
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