Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Natural And Engineered Nicking Endonucleases—from Cleavage Mechanism To Engineering Of Strand-specificity

S. Chan, B. Stoddard, S. Xu
Published 2011 · Biology, Computer Science, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4–8 bp palindromic sequences, have revealed a variety of mechanisms of molecular recognition and catalysis. Less well-studied are REases which cleave only one of the strands of dsDNA, creating a nick instead of a ds break. Naturally occurring nicking endonucleases (NEases) range from frequent cutters such as Nt.CviPII (^CCD; ^ denotes the cleavage site) to rare-cutting homing endonucleases (HEases) such as I-HmuI. In addition to these bona fida NEases, individual subunits of some heterodimeric Type IIS REases have recently been shown to be natural NEases. The discovery and characterization of more REases that recognize asymmetric sequences, particularly Types IIS and IIA REases, has revealed recognition and cleavage mechanisms drastically different from the canonical Type IIP mechanisms, and has allowed researchers to engineer highly strand-specific NEases. Monomeric LAGLIDADG HEases use two separate catalytic sites for cleavage. Exploitation of this characteristic has also resulted in useful nicking HEases. This review aims at providing an overview of the cleavage mechanisms of Types IIS and IIA REases and LAGLIDADG HEases, the engineering of their nicking variants, and the applications of NEases and nicking HEases.
This paper references
10.1016/0042-6822(75)90214-7
EcoRI endonuclease cleavage map of bacteriophage P4-DNA.
L. Goldstein (1975)
10.1093/NAR/GKG274
A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.
R. Roberts (2003)
10.1093/nar/gkn178
Crystal structures of I-SceI complexed to nicked DNA substrates: snapshots of intermediates along the DNA cleavage reaction pathway
C. M. Moure (2008)
10.1016/J.JMB.2004.07.032
DNA binding and cleavage by the HNH homing endonuclease I-HmuI.
B. Shen (2004)
10.1038/40446
Structure of the multimodular endonuclease FokI bound to DNA
D. A. Wah (1997)
10.1093/nar/gkm041
USER™ friendly DNA engineering and cloning method by uracil excision
J. Bitinaite (2007)
10.1016/j.molcel.2007.12.028
MeCP2 binding to DNA depends upon hydration at methyl-CpG.
K. Ho (2008)
10.1371/JOURNAL.PMED.0040103
A Prospective Study of Plasma Vitamin D Metabolites, Vitamin D Receptor Polymorphisms, and Prostate Cancer
H. Li (2007)
10.1128/JB.65.2.113-121.1953
Host controlled variation in bacterial viruses.
G. Bertani (1953)
10.1093/NAR/29.12.2492
The nicking endonuclease N.BstNBI is closely related to type IIs restriction endonucleases MlyI and PleI.
L. Higgins (2001)
Analysis of the symmetric metal-dependent I-CreI homing endonuclease mechanism
B Chevalier (2004)
10.1093/embo-reports/kve175
Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state
C. Besnier (2001)
10.1038/nsb853
Catalytic domain structure and hypothesis for function of GIY-YIG intron endonuclease I-TevI
P. Roey (2002)
10.1515/BC.2000.137
Characterization of the Specific DNA Nicking Activity of Restriction Endonuclease N.BstNBI
R. Morgan (2000)
10.1007/s12033-009-9143-2
Molecular Detection of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis
E. Dawson (2009)
Crystal structure of the beta beta alpha-Me type II restriction endonuclease Hpy99I with target DNA
M. Sokolowska (2009)
10.1016/0022-2836(70)90150-6
A restriction enzyme from Hemophilus influenzae: II. Base sequence of the recognition site
T. Kelly (1970)
10.1093/NAR/GKH951
Type II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily.
M. Saravanan (2004)
10.1021/bc900031a
Preparation of mammalian expression vectors incorporating site-specifically platinated-DNA lesions.
W. Ang (2009)
10.1016/J.JMB.2007.07.052
Coevolution of a homing endonuclease and its host target sequence.
M. Scalley-Kim (2007)
10.1021/BI036033J
Changing the enzymatic activity of T7 endonuclease by mutations at the beta-bridge site: alteration of substrate specificity profile and metal ion requirements by mutation distant from the catalytic domain.
C. Guan (2004)
10.1093/NAR/25.17.3379
Homing endonucleases: keeping the house in order.
M. Belfort (1997)
10.1002/CHIN.200934239
Artificial Restriction DNA Cutters as New Tools for Gene Manipulation
H. Katada (2009)
10.1134/S0006297906070157
Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex
A. Yunusova (2006)
10.1093/carcin/bgp103
Review and meta-analysis on vitamin D receptor polymorphisms and cancer risk.
S. Raimondi (2009)
10.1373/CLINCHEM.2007.091116
Isothermal DNA amplification with gold nanosphere-based visual colorimetric readout for herpes simplex virus detection.
Eric Tan (2007)
10.1073/PNAS.90.18.8499
Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.
A. Jeltsch (1993)
10.1021/ac902825r
Pyrosequencing on nicked dsDNA generated by nicking endonucleases.
Q. Song (2010)
10.1021/BI048970C
Metal-dependent DNA cleavage mechanism of the I-CreI LAGLIDADG homing endonuclease.
B. Chevalier (2004)
10.1186/1472-6807-7-48
Type II restriction endonuclease R.Eco29kI is a member of the GIY-YIG nuclease superfamily
Elena M. Ibryashkina (2007)
10.1073/pnas.082089499
Comprehensive human genome amplification using multiple displacement amplification
F. Dean (2002)
10.1016/S0092-8674(04)00301-0
RAG Proteins Shepherd Double-Strand Breaks to a Specific Pathway, Suppressing Error-Prone Repair, but RAG Nicking Initiates Homologous Recombination
G. S. Lee (2004)
10.1002/j.1460-2075.1995.tb00259.x
Intron‐encoded endonuclease I‐TevI binds as a monomer to effect sequential cleavage via conformational changes in the td homing site.
J. E. Mueller (1995)
10.1038/labinvest.3700311
Methylated-CpG island recovery assay: a new technique for the rapid detection of methylated-CpG islands in cancer
T. Rauch (2005)
10.1016/J.AB.2005.10.024
Significant enhancement of fluorescence on hybridization of a molecular beacon to a target DNA in the presence of a site-specific DNA nickase.
L. Zheleznaya (2006)
10.1016/S1359-6446(05)03694-9
SAGE and related approaches for cancer target identification.
D. Porter (2006)
10.1016/0378-1119(93)90738-O
Atypical DNA-binding properties of class-IIS restriction endonucleases: evidence for recognition of the cognate sequence by a FokI monomer.
P. Skowron (1993)
10.1017/S0033583505004063
Homing endonuclease structure and function.
B. Stoddard (2005)
10.1093/nar/gki921
A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease
C. Guan (2005)
10.1016/j.febslet.2006.09.075
Domain organization and metal ion requirement of the Type IIS restriction endonuclease MnlI
Edita Kriukienė (2006)
10.1073/PNAS.0507949102
Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease.
S. Grazulis (2005)
10.1002/cncr.23867
Vitamin D receptor polymorphisms and the risk of cutaneous melanoma
S. Mocellin (2008)
10.1093/NAR/GKH618
Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI.
G. Silva (2004)
10.1110/ps.03115604
HNH family subclassification leads to identification of commonality in the His‐Me endonuclease superfamily
P. Mehta (2004)
A novel mechanism for the scission of double-stranded DNA : BfiI cuts both 3 0 – 5 0 and 5 0 – 3 0 strands by rotating a single active site
G. Sasnauskas (2009)
10.1021/bi800746p
Specific versus nonspecific isothermal DNA amplification through thermophilic polymerase and nicking enzyme activities.
Eric Tan (2008)
10.1038/6716
Crystal structure of a phospholipase D family member
J. Stuckey (1999)
10.1002/j.1460-2075.1993.tb05956.x
Asymmetrical recognition and activity of the I‐SceI endonuclease on its site and on intron‐exon junctions.
A. Perrin (1993)
10.1016/S0092-8674(00)80976-9
Beyond Homing: Competition between Intron Endonucleases Confers a Selective Advantage on Flanking Genetic Markers
H. Goodrich-Blair (1996)
10.1074/JBC.M200357200
Mismatch Repair in Human Nuclear Extracts
H. Wang (2002)
10.1074/JBC.M108441200
Many Type IIs Restriction Endonucleases Interact with Two Recognition Sites before Cleaving DNA*
A. J. Bath (2002)
10.1074/JBC.270.51.30237
Mechanisms of Intron Mobility (*)
M. Belfort (1995)
Coevolution of homing endonuclease specificity and its host target sequence
M. Scalley-Kim (2007)
10.1016/J.JMB.2007.09.027
Strand-specific contacts and divalent metal ion regulate double-strand break formation by the GIY-YIG homing endonuclease I-BmoI.
J. Carter (2007)
10.1093/NAR/20.19.5085
Characterization of MeCP2, a vertebrate DNA binding protein with affinity for methylated DNA.
R. Meehan (1992)
10.1016/j.ab.2008.06.035
Targeting DNA 5mCpG sites with chimeric endonucleases.
A. Fomenkov (2008)
10.1016/S0006-291X(74)80181-6
Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.
D. Garfin (1974)
10.1002/cbic.200900040
Artificial Restriction DNA Cutters as New Tools for Gene Manipulation
H. Katada (2009)
10.1021/BI020467H
Catalytic mechanisms of restriction and homing endonucleases.
E. Galburt (2002)
10.1385/MB:19:2:133
Simple and rapid preparation of gapped plasmid DNA for incorporation of oligomers containing specific DNA lesions
H. Wang (2001)
10.1016/0378-1119(89)90034-6
Group I introns as mobile genetic elements: facts and mechanistic speculations--a review.
B. Dujon (1989)
10.1007/978-1-59745-483-4_3
A single-molecule barcoding system using nanoslits for DNA analysis : nanocoding.
K. Jo (2009)
10.1128/JB.186.13.4307-4314.2004
Group I intron homing in Bacillus phages SPO1 and SP82: a gene conversion event initiated by a nicking homing endonuclease.
M. Landthaler (2004)
10.1016/J.JMB.2003.12.012
Generation of the BfiI restriction endonuclease from the fusion of a DNA recognition domain to a non-specific nuclease from the phospholipase D superfamily.
M. Zaremba (2004)
In Pingoud,A. (ed.), Restriction Endonucleases
J. R. Horton (2004)
10.1016/0022-2836(70)90149-X
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
H. Smith (1970)
10.1093/NAR/GKH958
Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification.
S. Chan (2004)
10.1016/j.jmb.2008.07.010
Engineering variants of the I-SceI homing endonuclease with strand-specific and site-specific DNA-nicking activity.
Yan Niu (2008)
10.1046/J.1432-1033.2003.03518.X
In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional group I intron-encoded protein, I-AniI.
W. J. Geese (2003)
10.1093/NAR/GKG433
The nicking homing endonuclease I-BasI is encoded by a group I intron in the DNA polymerase gene of the Bacillus thuringiensis phage Bastille.
M. Landthaler (2003)
10.1016/S0022-2836(03)00020-2
The metal-independent type IIs restriction enzyme BfiI is a dimer that binds two DNA sites but has only one catalytic centre.
A. Lagunavičius (2003)
10.1093/nar/gkp228
Crystal structure of the ββα-Me type II restriction endonuclease Hpy99I with target DNA
M. Sokolowska (2009)
10.1073/PNAS.95.18.10564
Structure of FokI has implications for DNA cleavage.
D. A. Wah (1998)
10.1007/BF00269404
Physical mapping of the HindIII, EcoRI, Sal and Sma restriction endonuclease cleavage fragments from bacteriophage T5 DNA
A. V. Gabain (2004)
10.1016/j.pep.2009.09.003
Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA.
P. Zhang (2010)
10.1073/pnas.0730811100
Isothermal reactions for the amplification of oligonucleotides
J. Van Ness (2003)
10.1023/A:1026008528310
Cloning and Sequencing of the Gene of Site-Specific Nickase N.BspD6I
T. A. Perevyazova (2004)
10.1007/978-3-642-18851-0_6
The Integration of Recognition and Cleavage: X-Ray Structures of Pre-Transition State Complex, Post-Reactive Complex, and the DNA-Free Endonuclease
A. Grigorescu (2004)
10.1093/nar/gkp1194
A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site
G. Sasnauskas (2010)
10.1093/nar/gkm481
Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI
S. Xu (2007)
10.1093/emboj/20.14.3631
Intertwined structure of the DNA‐binding domain of intron endonuclease I‐TevI with its substrate
P. Van Roey (2001)
10.1093/NAR/GKH674
The isolation of strand-specific nicking endonucleases from a randomized SapI expression library.
J. Samuelson (2004)
10.1093/NAR/26.4.1084
Cloning and analysis of the four genes coding for Bpu10I restriction-modification enzymes.
K Stankevicius (1998)
10.1007/978-1-59745-454-4_6
DeepSAGE: higher sensitivity and multiplexing of samples using a simpler experimental protocol.
K. Nielsen (2008)
10.1093/NAR/23.13.2519
The site-specific DNA endonuclease encoded by a group I intron in the Chlamydomonas pallidostigmatica chloroplast small subunit rRNA gene introduces a single-strand break at low concentrations of Mg2+
M. Turmel (1995)
10.1093/nar/gkn1014
Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
Taku Murakami (2009)
10.1093/nar/gkp046
Targeting individual subunits of the FokI restriction endonuclease to specific DNA strands
Kelly L Sanders (2009)
10.1016/J.PEP.2006.04.002
Cloning of Nt.CviQII nicking endonuclease and its cognate methyltransferase: M.CviQII methylates AG sequences.
S. Chan (2006)
Cloning and sequencing of the gene of site - specific nickase N . BspD 6 I
A. K. Yunusova (2003)
10.1007/978-3-642-18851-0_13
Role of Metal Ions in Promoting DNA Binding and Cleavage by Restriction Endonucleases
J. Cowan (2004)
10.1074/jbc.M506775200
Mva1269I: A Monomeric Type IIS Restriction Endonuclease from Micrococcus Varians with Two EcoRI- and FokI-like Catalytic Domains*
Elena Armalyte (2005)
10.1371/journal.pone.0003495
The Long March: A Sample Preparation Technique that Enhances Contig Length and Coverage by High-Throughput Short-Read Sequencing
Katherine Sorber (2008)
10.1016/j.jmb.2008.09.033
Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit.
G. Kachalova (2008)
10.1016/J.JMB.2005.02.034
Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI.
D. F. Heiter (2005)
10.1016/j.tibtech.2009.11.007
Zinc finger nuclease technology heralds a new era in mammalian transgenesis.
F. le Provost (2010)
10.1038/6683
Structural and mechanistic basis of immunity toward endonuclease colicins
C. Kleanthous (1999)
10.1016/J.JMB.2006.02.054
I-BasI and I-HmuI: two phage intron-encoded endonucleases with homologous DNA recognition sequences but distinct DNA specificities.
M. Landthaler (2006)
10.1128/JVI.01737-06
A High-Throughput Method for Cloning and Sequencing Human Immunodeficiency Virus Type 1 Integration Sites
S. Kim (2006)
10.1073/pnas.0611151104
A single-molecule barcoding system using nanoslits for DNA analysis
K. Jo (2007)
10.1073/pnas.0810588106
Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease
Audrey McConnell Smith (2009)
10.1002/0470028637.MET268
Handbook of metalloproteins
A. Messerschmidt (2006)
10.1093/nar/gkl1044
Rapid DNA mapping by fluorescent single molecule detection
M. Xiao (2007)
10.1128/JVI.48.1.18-30.1983
In vivo cleavage of cytosine-containing bacteriophage T4 DNA to genetically distinct, discretely sized fragments.
K. Carlson (1983)
10.1006/JMBI.2001.4635
FokI requires two specific DNA sites for cleavage.
É. Vanamee (2001)
10.1016/0092-8674(90)90174-D
A self-splicing group I intron in the DNA polymerase gene of bacillus subtilis bacteriophage SPO1
H. Goodrich-Blair (1990)
10.1016/0378-1119(91)90345-C
Class-IIS restriction enzymes--a review.
W. Szybalski (1991)
Analysis of the symmetric metal-dependent I-CreI homing endonuclease
B. Chevalier (2004)
10.1093/nar/gkp1092
Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs
P. Too (2010)
10.1128/JB.64.4.557-569.1952
A nonhereditary, host-induced variation of bacterial viruses.
S. Luria (1952)
10.1074/jbc.M005929200
The Minimal Repression Domain of MBD2b Overlaps with the Methyl-CpG-binding Domain and Binds Directly to Sin3A*
J. Boeke (2000)
Generation of a nicking enzyme that stimulates site-specific gene 16 Nucleic Acids Research, 2011, Vol. 39, No. 1 conversion from the I-AniI LAGLIDADG homing endonuclease
A. McConnell Smith (2009)
10.1186/1471-2164-7-30
SNP-RFLPing: restriction enzyme mining for SNPs in genomes
Hsueh-Wei Chang (2005)
10.1016/S0092-8674(85)80011-8
An intron-encoded protein is active in a gene conversion process that spreads an intron into a mitochondrial gene
A. Jacquier (1985)
10.1073/pnas.241215698
Engineering a nicking endonuclease N.AlwI by domain swapping
Y. Xu (2001)
10.1093/NAR/29.18.3757
Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.
B. Chevalier (2001)
10.1016/J.JMB.2004.02.003
Engineering strand-specific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI.
Z. Zhu (2004)
10.1038/sj.emboj.7601104
When a helicase is not a helicase: dsDNA tracking by the motor protein EcoR124I
Louise K. Stanley (2006)
10.1073/PNAS.95.18.10570
FokI dimerization is required for DNA cleavage.
J. Bitinaite (1998)
Class-IIS restriction enzymes–a
W. Szybalski (1991)
Cloning and sequencing of the gene of site-specific nickase
T. A. Perevyazova (2003)
10.1146/ANNUREV.BI.62.070193.003103
Introns as mobile genetic elements.
A. Lambowitz (1993)
10.1073/pnas.0608689104
Site-specific DNA transesterification catalyzed by a restriction enzyme
G. Sasnauskas (2007)
10.1186/1472-6807-8-48
Type II restriction endonuclease R.Hpy188I belongs to the GIY-YIG nuclease superfamily, but exhibits an unusual active site
Katarzyna H. Kaminska (2008)
10.1038/sj.emboj.7601672
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD‐(D/E)‐XK motif
L. Zhao (2007)
10.1016/j.ab.2009.02.020
Preparation of heteroduplex enhanced green fluorescent protein plasmid for in vivo mismatch repair activity assay.
Bisheng Zhou (2009)
10.1021/AC051364I
Isothermal DNA amplification coupled with DNA nanosphere-based colorimetric detection.
Eric Tan (2005)
10.1038/nbt1319
An improved zinc-finger nuclease architecture for highly specific genome editing
J. C. Miller (2007)
10.1038/86181
The homing endonuclease I-CreI uses three metals, one of which is shared between the two active sites
B. Chevalier (2001)
10.1016/S0022-2836(03)00447-9
Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.
B. Chevalier (2003)
10.1073/pnas.1131003100
How the BfiI restriction enzyme uses one active site to cut two DNA strands
G. Sasnauskas (2003)
10.1073/pnas.0500923102
How restriction enzymes became the workhorses of molecular biology
R. Roberts (2005)
10.1093/nar/gkp1223
A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI
Benjamin P. Kleinstiver (2010)
10.1002/ANIE.200501262
A free-running DNA motor powered by a nicking enzyme.
J. Bath (2005)
10.1007/s11033-008-9391-4
In vitro detection of methylated DNA via recombinant protein MBD2b
M. Foedermayr (2008)
10.1093/nar/gkn107
Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases
H. Kuhn (2008)
10.1242/dev.001131
Genome-wide analysis of DNA methylation patterns
D. Zilberman (2007)
10.1093/nar/gkp643
Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease
G. P. van Nierop (2009)
10.1093/nar/gkm654
Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
Traci L. Kiesling (2007)
10.1016/S0092-8674(00)81887-5
MeCP2 Is a Transcriptional Repressor with Abundant Binding Sites in Genomic Chromatin
Xinsheng Nan (1997)
10.1261/rna.761708
A novel sequence-specific RNA quantification method using nicking endonuclease, dual-labeled fluorescent DNA probe, and conformation-interchangeable oligo-DNA.
Kazufumi Hosoda (2008)
10.1093/nar/gkn033
Enzymatic signal amplification of molecular beacons for sensitive DNA detection
J. Li (2008)
10.1021/BI00740A018
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
P. Sharp (1973)
10.1016/J.JMB.2003.09.068
The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity.
C. M. Moure (2003)
10.1016/J.JMB.2005.02.035
Cleavage of individual DNA strands by the different subunits of the heterodimeric restriction endonuclease BbvCI.
S. Bellamy (2005)



This paper is referenced by
10.4155/fmc.11.139
Towards artificial metallonucleases for gene therapy: recent advances and new perspectives.
B. Gyurcsik (2011)
Gene targeting at and distant from DNA breaks in yeast and human cells
Samantha Stuckey (2013)
A new molecular diagnosis method combined single primer isothermal amplification with rapid isothermal detection assay in detection of group B Streptococcus
Guo Xin-zhong (2013)
10.1016/j.dnarep.2015.11.016
Evolution of the methyl directed mismatch repair system in Escherichia coli.
C. Putnam (2016)
10.1101/2020.03.06.979674
Neuron-specific increase in lamin B1 disrupts nuclear function in Huntington’s disease
Rafael Alcalá-Vida (2020)
10.1016/j.plasmid.2011.07.007
Vectors for ligation-independent construction of lacZ gene fusions and cloning of PCR products using a nicking endonuclease.
Carrie J Oster (2011)
10.1101/424408
Comparison of HSV-1 strains circulating in Finland demonstrates the uncoupling of whole-genome relatedness and phenotypic outcomes of viral infection
Christopher D. Bowen (2019)
10.1093/nar/gks1355
Imprinting at the PLAGL1 domain is contained within a 70-kb CTCF/cohesin-mediated non-allelic chromatin loop
Isabel Iglesias-Platas (2013)
10.1515/bmc-2015-0016
Sequence-specific DNA nicking endonucleases
S. Xu (2015)
10.1016/j.meegid.2014.12.014
Prevalence and complete genome characterization of turkey picobirnaviruses
H. Verma (2015)
10.1016/j.bpj.2017.02.041
Physical and Functional Characterization of a Viral Genome Maturation Complex.
Teng-Chieh Yang (2017)
10.1093/bioinformatics/btw183
MOCAT2: a metagenomic assembly, annotation and profiling framework
Jens Roat Kultima (2016)
10.1093/nar/gks1043
Natural zinc ribbon HNH endonucleases and engineered zinc finger nicking endonuclease
S. Xu (2013)
10.1515/sagmb-2016-0066
Comparing the performance of linear and nonlinear principal components in the context of high-dimensional genomic data integration
Shofiqul Islam (2017)
10.1101/317479
Sensitive detection of pre-integration intermediates of LTR retrotransposons in crop plants
Jungnam Cho (2018)
10.2174/1876402911103040284
Triggered, Nanostructured Biodegradables (TNBs) for Surgical Implants
R. Gordon (2011)
10.1093/nar/gkr453
Concerted action at eight phosphodiester bonds by the BcgI restriction endonuclease
Jacqueline J. T. Marshall (2011)
10.1371/journal.pone.0180493
Characterization of the emerging zoonotic pathogen Arcobacter thereius by whole genome sequencing and comparative genomics
Francesca Rovetto (2017)
10.1038/srep27740
Rising levels of atmospheric oxygen and evolution of Nrf2
R. Gacesa (2016)
10.1039/c7nr08900g
Black phosphorus nanosheets for rapid microRNA detection.
J. Zhou (2018)
10.1016/j.bios.2017.06.004
Current trends in electrochemical sensing and biosensing of DNA methylation.
Ludmila Krejcova (2017)
10.1093/nar/gkv1238
AREsite2: an enhanced database for the comprehensive investigation of AU/GU/U-rich elements
J. Fallmann (2016)
10.1128/mSystems.00029-16
Long-Term Evolution of Burkholderia multivorans during a Chronic Cystic Fibrosis Infection Reveals Shifting Forces of Selection
I. Silva (2016)
10.1093/nar/gkq1351
Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease
Georgij Kostiuk (2011)
10.1371/journal.pone.0023804
Divalent Metal Ion Differentially Regulates the Sequential Nicking Reactions of the GIY-YIG Homing Endonuclease I-BmoI
Benjamin P. Kleinstiver (2011)
10.1039/c7cc03074f
On the binding modes of metal NHC complexes with DNA secondary structures: implications for therapy and imaging.
Özden Karaca (2017)
10.1017/S1351324912000125
Recent advances in methods of lexical semantic relatedness – a survey
Z. Zhang (2012)
10.5897/AJMR12.1389
New molecular diagnosis method combined with nucleic acid sequence-based amplification and probe amplification in rapid detection of Enterovirus 71
Ming Hong (2012)
10.1155/2015/546851
Configuration Transitions of Free Circular DNA System Induced by Nicks
Chao Ji (2015)
10.1128/mBio.00969-14
Comparison of Widely Used Listeria monocytogenes Strains EGD, 10403S, and EGD-e Highlights Genomic Differences Underlying Variations in Pathogenicity
C. Bécavin (2014)
10.1387/IJDB.160139OH
Single-molecule, antibody-free fluorescent visualisation of replication tracts along barcoded DNA molecules.
Francesco De Carli (2016)
10.1016/j.jbiotec.2017.05.027
The completely annotated genome and comparative genomics of the Peptoniphilaceae bacterium str. ING2-D1G, a novel acidogenic bacterium isolated from a mesophilic biogas reactor.
Geizecler Tomazetto (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar