← Back to Search
Genome-wide Analysis Of Differentially Expressed Genes During The Early Stages Of Tomato Infection By A Potyvirus.
P. Alfenas-Zerbini, I. G. Maia, R. D. Fávaro, J. Cascardo, S. Brommonschenkel, F. M. Zerbini
Published 2009 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Plant responses against pathogens cause up- and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from inoculated leaves at 72 h after inoculation. Several genes were identified as upregulated, including genes involved in plant defense responses (e.g., pathogenesis-related protein 5), regulation of the cell cycle (e.g., cytokinin-repressed proteins), signal transduction (e.g., CAX-interacting protein 4, SNF1 kinase), transcriptional regulators (e.g., WRKY and SCARECROW transcription factors), stress response proteins (e.g., Hsp90, DNA-J, 20S proteasome alpha subunit B, translationally controlled tumor protein), ubiquitins (e.g., polyubiquitin, ubiquitin activating enzyme 2), among others. Downregulated genes were also identified, which likewise display identity with genes involved in several metabolic pathways. Differential expression of selected genes was validated by macroarray analysis and quantitative real-time polymerase chain reaction. The possible roles played by some of these genes in the viral infection cycle are discussed.
This paper references
Tobacco mosaic virus induced alterations in the gene expression profile of Arabidopsis thaliana.
S. Golem (2003)
The ubiquitin 26S proteasome proteolytic pathway.
J. Smalle (2004)
Signaling in plant-microbe interactions.
B. Baker (1997)
Molecular mapping of the viral determinants of systemic wilting induced by a Lettuce mosaic virus (LMV) isolate in some lettuce cultivars.
R. Krause-Sakate (2005)
Distinct features of Pepper yellow mosaic virus isolates from tomato and sweetpepper
L. D. Cunha (2004)
Potato spindle tuber viroid as inducer of RNA silencing in infected tomato.
A. Itaya (2001)
Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor.
B. Cooper (2001)
Potato virus Y induced changes in the gene expression of potato (Solanum tuberosum L.)
M. Pompe-Novak (2005)
Systemic Gene Expression in Arabidopsis during an Incompatible Interaction with Alternaria brassicicola1[w]
P. Schenk (2003)
Murine Cytomegalovirus m142 and m143 Are both Required To Block Protein Kinase R-Mediated Shutdown of Protein Synthesis
Ralitsa S Valchanova (2006)
Global impact: elucidating plant responses to viral infection.
S. Whitham (2006)
Pea embryonic tissues show common responses to the replication of a wide range of viruses.
M. Escaler (2000)
Induction of HSP70 and polyubiquitin expression associated with plant virus replication.
M. Aranda (1996)
Systemic acquired resistance.
W. E. Durrant (2004)
Tomato Ve disease resistance genes encode cell surface-like receptors
L. Kawchuk (2001)
P 1 / HC-Pro , a Viral Suppressor of RNA Silencing , Interferes with Arabidopsis Development and miRNA
K. Kasschau (2003)
Evasion of Cellular Antiviral Responses by Human Cytomegalovirus TRS1 and IRS1
S. J. Child (2004)
Double-Stranded RNA Binding May Be a General Plant RNA Viral Strategy To Suppress RNA Silencing
Zsuzsanna Mérai (2006)
The translationally controlled tumour protein (TCTP).
Ulrich-Axel Bommer (2004)
HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activities.
L. Ballut (2005)
Identidade e propriedades de isolados de potyvírus provenientes de Capsicum spp.
A. Truta (2004)
Identity and properties of potyvirus isolates obtained from Capsicum spp
A. A. C. Truta (2004)
Double-Stranded RNA Binding by a Heterodimeric Complex of Murine Cytomegalovirus m142 and m143 Proteins
S. J. Child (2006)
P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction.
K. Kasschau (2003)
Basic local alignment search tool.
S. Altschul (1990)
Interaction between Long-Distance Transport Factor and Hsp70-Related Movement Protein of Beet Yellows Virus
A. I. Prokhnevsky (2002)
Reprogramming plant gene expression: a prerequisite to geminivirus DNA replication.
L. Hanley-Bowdoin (2004)
Translation initiation and viral tricks.
R. Schneider (2003)
Beet yellows closterovirus HSP70-like protein mediates the cell-to-cell movement of a potexvirus transport-deficient mutant and a hordeivirus-based chimeric virus.
A. Agranovsky (1998)
Capsid Protein-Mediated Recruitment of Host DnaJ-Like Proteins Is Required for Potato Virus Y Infection in Tobacco Plants
D. Hofius (2007)
Expression of potyviral polyproteins in transgenic plants reveals three proteolytic activities required for complete processing.
J. C. Carrington (1990)
Genetics of resistance to the geminivirus, Bean dwarf mosaic virus, and the role of the hypersensitive response in common bean
Y.-S. Seo (2003)
A calmodulin-related protein that suppresses posttranscriptional gene silencing in plants.
R. Anandalakshmi (2000)
Effects of Calreticulin on Viral Cell-to-Cell Movement1
Min-Huei Chen (2005)
SYSTEMIC ACQUIRED RESISTANCE IN POTATO
D. J. Navarre (2003)
Mutation of Host dnaJ Homolog Inhibits Brome Mosaic Virus Negative-Strand RNA Synthesis
Y. Tomita (2003)
Cell‐to‐cell movement and assembly of a plant closterovirus: roles for the capsid proteins and Hsp70 homolog
D. Alzhanova (2001)
Diverse RNA viruses elicit the expression of common sets of genes in susceptible Arabidopsis thaliana plants.
S. Whitham (2003)
Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus.
Mónica Gandía (2007)
Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus
David B Kushner (2003)
The I2C family from the wilt disease resistance locus I2 belongs to the nucleotide binding, leucine-rich repeat superfamily of plant resistance genes.
N. Ori (1997)
Specific and common changes in Nicotiana benthamiana gene expression in response to infection by enveloped viruses.
G. Senthil (2005)
Spatial analysis of arabidopsis thaliana gene expression in response to Turnip mosaic virus infection.
C. Yang (2007)
Induction and suppression of RNA silencing: insights from viral infections
O. Voinnet (2005)
A Counterdefensive Strategy of Plant Viruses Suppression of Posttranscriptional Gene Silencing
K. D. Kasschau (1998)
Retracted: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
G. Brigneti (1998)
Antiviral Immunity Directed by Small RNAs
S. Ding (2007)
A viral suppressor of gene silencing in plants.
R. Anandalakshmi (1998)
Transcript profiling in host-pathogen interactions.
R. Wise (2007)
H. Peretz (1966)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
Activation of heat-shock response by an adenovirus is essential for virus replication
J. B. Glotzer (2000)
C. W. Collmer (2000)
New Advances in Understanding the Molecular Biology of Plant/Potyvirus Interactions
F. Revers (1999)
Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translation and cylindrical inclusion protein as a product of the potyvirus genome.
W. G. Dougherty (1980)
Pepper yellow mosaic virus, a new potyvirus in sweetpepper, Capsicum annuum
A. Inoue-Nagata (2002)
The mRNA of the translationally controlled tumor protein P23/TCTP is a highly structured RNA, which activates the dsRNA-dependent protein kinase PKR.
Ulrich-Axel Bommer (2002)
The I gene of bean: a dosage-dependent allele conferring extreme resistance, hypersensitive resistance, or spreading vascular necrosis in response to the potyvirus Bean common mosaic virus.
C. Collmer (2000)
The response of the poplar transcriptome to wounding and subsequent infection by a viral pathogen
C. M. Smith (2004)
A viral suppressor of RNA silencing differentially regulates the accumulation of short interfering RNAs and micro-RNAs in tobacco
A. Mallory (2002)
Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato.
T. H. Tai (1999)
This paper is referenced by
Proteomic analysis of Arabidopsis thaliana leaves infested by tobacco whitefly Bemisia tabaci (Gennadius) B biotype
Haidi Yin (2011)
Etude de l'activité RNasique associée au protéasome 20S chez Arabidopsis thaliana : un rôle dans la dégradation des ARN viraux ?
Anne-Sophie Dielen (2009)
Geminiviral Co-option of Post-Translational Modification Pathways
Rosa Lozano-Durán (2014)
Involvement of Translationally controlled tumor protein in Tomato yellow spot virus infection
P. O. Andrade (2017)
Translationally controlled tumour protein (TCTP) from tomato and Nicotiana benthamiana is necessary for successful infection by a potyvirus.
F. P. Bruckner (2017)
The receptor-like kinase SlSOBIR1 is differentially modulated by virus infection but its overexpression in tobacco has no significant impact on virus accumulation
Alessandra Tenório Costa (2015)
Impacts of the overexpression of a tomato translationally controlled tumor protein (TCTP) in tobacco revealed by phenotypic and transcriptomic analysis
M. Carvalho (2017)
Proteome of resistant and susceptible Passiflora species in the interaction with cowpea aphid-borne mosaic virus reveals distinct responses to pathogenesis
Beatriz Carvalho (2019)
AN INVESTIGATION OF MOLECULAR INDICATORS OF PLANT VIRUS INFECTION
Sonia T Lilly (2014)
Pepino mosaic virus: an endemic pathogen of tomato crops
I. Hanssen (2010)
The WRKY transcription factor WRKY8 promotes resistance to pathogen infection and mediates drought and salt stress tolerance in Solanum lycopersicum.
Yong-Feng Gao (2019)
Vírus latentes aumentam a suscetibilidade de macieira cv. Maxi Gala à mancha foliar de Glomerella.
S. Alves (2016)
Abiotic stress responses promote Potato virus A infection in Nicotiana benthamiana.
Taina Suntio (2012)
Genome-wide Identification and Structural, Functional and Evolutionary Analysis of WRKY Components of Mulberry
Vinay Kumar Baranwal (2016)
Influence of an m-type thioredoxin in maize on potyviral infection
Y. Shi (2011)
Complete genome sequence of pepper yellow mosaic virus, a potyvirus, occurring in Brazil
N. Lucinda (2012)
Identification of differentially expressed genes in response to infection of a mild Citrus tristeza virus isolate in Citrus aurantifolia by suppression subtractive hybridization
Yong Cheng Liu (2012)
Proteomic identification of differentially expressed proteins in Gossypium thurberi inoculated with cotton Verticillium dahliae.
Fu'an Zhao (2012)
Identification and Expression Analysis of Genes Induced in Response to Tomato chlorosis virus Infection in Tomato
Mehtap Şahin-Çevik (2019)
Molecular and cellular mechanisms underlying potyvirus infection.
K. I. Ivanov (2014)
Sequences and transcriptional analysis of Coffea arabica var. Caturra and Coffea liberica plant responses to coffee berry borer Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) attack
Sandra M. Idárraga (2012)
Reference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus Pathosystems
A. L. Lacerda (2015)
Microarray Analysis of Tomato Plants Exposed to the Nonviruliferous or Viruliferous Whitefly Vector Harboring Pepper golden mosaic virus
R. O. Musser (2014)
Roles of the Translationally Controlled Tumor Protein (TCTP) in Plant Development.
Léo Betsch (2017)
DEFENCE GENE EXPRESSION IN THE TOMATO-VERTICILLIUM PATHOSYSTEM
C. D. M. Castroverde (2010)
Ribosome profiles and riboproteomes of healthy and Potato virus A‐ and Agrobacterium‐infected Nicotiana benthamiana plants
K. Eskelin (2019)
Dynamic transcriptome profiling of Bean Common Mosaic Virus (BCMV) infection in Common Bean (Phaseolus vulgaris L.)
Kathleen M. Martin (2016)
Signaling and Communication in Plants
S. Mugnai (2011)
Molecular cloning and characterization of a cassava translationally controlled tumor protein gene potentially related to salt stress response
Ailton B. Santa Brígida (2014)
Identification and validation of reference genes for normalization of transcripts from virus-infected Arabidopsis thaliana.
Sonia T Lilly (2011)
Proteomics changes during the incompatible interaction between cowpea and Colletotrichum gloeosporioides (Penz.) Penz and Sacc.
H. Moura (2014)
Use of RNA-seq data to identify and validate RT-qPCR reference genes for studying the tomato-Pseudomonas pathosystem
M. A. Pombo (2017)See more