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Macrophage Endocytosis Of Superparamagnetic Iron Oxide Nanoparticles: Mechanisms And Comparison Of Ferumoxides And Ferumoxtran-10

I. Raynal, P. Prigent, S. Peyramaure, A. Najid, Cécile Rebuzzi, C. Corot
Published 2004 · Chemistry, Medicine

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Rationale and objectivesSuperparamagnetic iron oxides (SPIO) used as magnetic resonance (MR) contrast agents undergo specific uptake by macrophages. The purpose of this study was first to determine the mechanism of macrophage uptake for Ferumoxides by using competition experiments with specific ligands of scavenger receptors SR-A (I/II) and second, to evaluate and compare the internalization of 2 different contrast agents, Ferumoxides (SPIO) and Ferumoxtran-10 (USPIO: ultrasmall superparamagnetic iron oxide) using macrophages obtained by chemical activation of human monocytic cells. MethodsFerumoxides and Ferumoxtran-10 are 2 MR contrast agents, composed of dextran-coated iron oxide nanoparticles. The endocytosis pathway of Ferumoxides was studied using competition experiments on mouse peritoneal macrophages in the presence of specific ligands of scavenger receptors SR-A (types I and II): polyinosinic acid and fucoidan. In vitro assays using THP-1 (human promonocyte) cells activated into macrophages were performed in the presence of the 2 superparamagnetic nanoparticles. The cellular uptake was determined by measuring the iron content using ICP-AES (inductively coupled plasma–atomic emission spectrometry) and by Prussian blue staining. ResultsIn the presence of polyinosinic acid or fucoidan, the endocytosis of Ferumoxides by mouse peritoneal macrophages was inhibited. This inhibition was obtained using 10 &mgr;g/mL of scavenger receptor ligands at a concentration of 62.5 &mgr;g Fe/mL of SPIO, and a dose-dependent relationship was observed. Without competitors, the percentage of uptake of Ferumoxides by mouse peritoneal macrophages ranged between 3 and 8%. On the human activated monocyte THP-1 cell assay, Ferumoxides underwent a higher macrophage uptake (between 1.1 and 3%) compared with Ferumoxtran-10 (between 0.03 and 0.12%). This difference is attributed to the larger size of Ferumoxides nanoparticles. ConclusionsCompetition experiments indicate that the cellular uptake of Ferumoxides involves scavenger receptor SR-A-mediated endocytosis. The comparison between Ferumoxides and Ferumoxtran-10 confirms that macrophage uptake of iron oxide nanoparticles depends mainly on the size of these contrast agents.
This paper references
Identification of a small-molecule, nonpeptide macrophage scavenger receptor antagonist.
P. Lysko (1999)
10.1097/00041433-199710000-00006
The other side of scavenger receptors: pattern recognition for host defense
M. Krieger (1997)
10.1002/IJC.2910260208
Establishment and characterization of a human acute monocytic leukemia cell line (THP‐1)
S. Tsuchiya (1980)
10.4324/9780203967621-26
Transport of Macromolecules
D. Hames (2006)
10.1161/01.CIR.103.3.415
Magnetic Resonance Imaging of Atherosclerotic Plaque With Ultrasmall Superparamagnetic Particles of Iron Oxide in Hyperlipidemic Rabbits
S. Ruehm (2001)
Correlation between clinical status and macrophage activity imaging in rats central nervous system
V Dousset (2002)
10.1002/JMRI.1880070140
Magnetically labeled cells can be detected by MR imaging
R. Weissleder (1997)
10.1097/00004424-199802000-00001
Detection of focal hepatic lesions: effects of superparamagnetic iron oxide (AMI-25) on magnetic resonance imaging of the liver using T2-weighted fast spin-echo sequences and gradient-and-spin-echo sequences at 1.0 tesla.
G. Jung (1998)
10.1148/RADIOLOGY.217.3.R00DC04819
Nephrotoxic nephritis and obstructive nephropathy: evaluation with MR imaging enhanced with ultrasmall superparamagnetic iron oxide-preliminary findings in a rat model.
O. Hauger (2000)
Efficacy of superparamagnetic iron oxide as an adjunct to MR imaging of the liver: results of a multicenter trial
P R Ros (1993)
Detection of liver metastasis with superparamagnetic iron oxide in 15 patients: results of MR imaging at 1.5 T
G Marchal (1989)
10.3109/10611869808997890
Superparamagnetic agents in magnetic resonance imaging: physicochemical characteristics and clinical applications. A review.
B. Bonnemain (1998)
10.1148/RADIOLOGY.193.3.7972805
Hepatic tumors: detection and characterization at 1-T MR imaging enhanced with AMI-25.
A. Denys (1994)
10.1097/00004424-199510000-00006
Cellular Uptake and Trafficking of a Prototypical Magnetic Iron Oxide Label In Vitro
E. Schulze (1995)
10.1016/S1074-5521(98)90156-9
Scavenger receptors: diverse activities and promiscuous binding of polyanionic ligands.
N. Platt (1998)
10.1016/S0009-8981(99)00101-1
Scavenger receptors and oxidized low density lipoproteins.
B. S. Dhaliwal (1999)
10.1006/EXCR.1998.4110
Size of IgG-opsonized particles determines macrophage response during internalization.
Michael Koval (1998)
10.1161/01.CIR.0000068315.98705.CC
Accumulation of Ultrasmall Superparamagnetic Particles of Iron Oxide in Human Atherosclerotic Plaques Can Be Detected by In Vivo Magnetic Resonance Imaging
M. E. Kooi (2003)
10.1148/RADIOLOGY.169.2.3174987
Superparamagnetic iron oxide: enhanced detection of focal splenic tumors with MR imaging.
R. Weissleder (1988)
10.1002/JMRI.1880040313
Phase I clinical evaluation of a new iron oxide MR contrast agent
S. McLachlan (1994)
10.1002/(SICI)1522-2594(199901)41:1<156::AID-MRM22>3.0.CO;2-C
MR imaging of intrarenal macrophage infiltration in an experimental model of nephrotic syndrome
O. Hauger (1999)
10.1016/0730-725X(95)00024-B
Physical and chemical properties of superparamagnetic iron oxide MR contrast agents: ferumoxides, ferumoxtran, ferumoxsil.
C. W. Jung (1995)
10.1146/ANNUREV.BI.63.070194.003125
Structures and functions of multiligand lipoprotein receptors: macrophage scavenger receptors and LDL receptor-related protein (LRP).
M. Krieger (1994)
Biophysical chemistry of membrane functions
A. Kotyk (1988)
10.1002/jmri.1194
Magnetic resonance imaging of atherosclerotic plaques using superparamagnetic iron oxide particles
S. A. Schmitz (2001)
10.1007/s000180050191
Scavenger receptor family proteins: roles for atherosclerosis, host defence and disorders of the central nervous system
Y. Yamada (1998)
10.2214/AJR.152.4.771
Detection of liver metastases with superparamagnetic iron oxide in 15 patients: results of MR imaging at 1.5 T.
G. Marchal (1989)
10.1056/NEJMOA022749
Noninvasive detection of clinically occult lymph-node metastases in prostate cancer.
M. Harisinghani (2003)
Expression cloning of SR-BI, a CD36-related class B scavenger receptor.
S. Acton (1994)
10.1161/01.ATV.20.2.290
Macrophage scavenger receptor class A: A multifunctional receptor in atherosclerosis.
M. D. de Winther (2000)
10.1128/9781555817671.CH4
The biology of macrophages.
D. Hume (1985)
Structures and functions of multiligand lipoprotein receptors: macrophage scavenger receptor and LDL receptor-related protein
M Krieger (1994)
10.1002/(SICI)1522-2586(199909)10:3<461::AID-JMRI30>3.0.CO;2-5
RES‐specific imaging of the liver and spleen with iron oxide particles designed for blood pool MR‐angiography
C. Bremer (1999)
10.1016/S0168-3659(00)00302-3
Dextrans for targeted and sustained delivery of therapeutic and imaging agents.
R. Mehvar (2000)
10.1016/S1076-6332(96)80560-5
Superparamagnetic agents: physicochemical characteristics and preclinical imaging evaluation.
S. Benderbous (1996)
Efficacy of superparamagnetic iron oxide as an adjunct to MR imaging of the liver: results of a multicenter trial [Abstract]. Radiology 1993;189(P):202
PR Ros (1993)
10.1148/RADIOLOGY.168.2.3393649
Superparamagnetic iron oxide: clinical application as a contrast agent for MR imaging of the liver.
D. Stark (1988)
10.1016/S1076-6332(03)80426-9
Correlation between clinical status and macrophage activity imaging in the central nervous system of rats.
V. Dousset (2002)
10.1016/0304-4165(86)90020-6
Pinocytosis and phagocytosis: the effect of size of a particulate substrate on its mode of capture by rat peritoneal macrophages cultured in vitro.
M. Pratten (1986)
10.1002/JBM.820280111
Macrophage/particle interactions: effect of size, composition and surface area.
A. Shanbhag (1994)
10.1016/S0047-6374(99)00079-2
Structure and function of type I and II macrophage scavenger receptors
H. Shirai (1999)
Detection of liver metastasis with superparamagnetic iron oxide in 15 patients: results of MR imaging
G Marchal (1989)



This paper is referenced by
10.1634/stemcells.2007-0251
Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking
M. Neri (2008)
Biofunctional materials for the modulation of macrophage phenotype and polarization
Shann S. Yu (2012)
10.1159/000098484
Magnetic Resonance Imaging of Ruptured Plaques in the Rabbit with Ultrasmall Superparamagnetic Particles of Iron Oxide
E. Durand (2007)
10.1002/jmri.22756
An experimental study on MR imaging of atherosclerotic plaque with SPIO marked endothelial cells in a rabbit model
Q. Zhou (2011)
10.1385/1-59745-011-1:457
Approaches to In Vivo Imaging of Cancer Immunotherapy
G. Vielhauer (2006)
Interfacial Design and Protein Engineering as Tools of Biomedical Nanotechnology in the Optimization of Protein Detecting Field Effect Transistors
T. Nicholson (2010)
10.1016/j.imlet.2013.12.018
Nanomedicine in autoimmunity.
X. Clemente-Casares (2014)
10.1002/wnan.158
Emerging applications of nanotechnology for the diagnosis and management of vulnerable atherosclerotic plaques.
Shann S. Yu (2011)
10.1016/j.actbio.2011.06.044
Targeted delivery of mannan-coated superparamagnetic iron oxide nanoparticles to antigen-presenting cells for magnetic resonance-based diagnosis of metastatic lymph nodes in vivo.
H. Vu-Quang (2011)
10.15406/jnmr.2017.06.00147
How to Look Closely to Vulnerable Atherosclerotic Plaques Using Nanoparticles
Cátia C Oliveira (2017)
10.1007/s12149-011-0565-0
Comparison of animal studies between interstitial magnetic resonance lymphography and radiocolloid SPECT/CT lymphoscintigraphy in the head and neck region
N. Kitamura (2012)
10.3892/mmr.2016.5874
A novel blood-pooling MR contrast agent: Carboxymethyl-diethylaminoethyl dextran magnetite.
A. Sonoda (2016)
10.3389/fonc.2019.00059
Functionalized Superparamagnetic Iron Oxide Nanoparticles (SPIONs) as Platform for the Targeted Multimodal Tumor Therapy
C. Janko (2019)
10.1016/j.colsurfb.2015.03.021
Dextrin-coated zinc substituted cobalt-ferrite nanoparticles as an MRI contrast agent: In vitro and in vivo imaging studies.
N. Sattarahmady (2015)
10.1002/nbm.1260
Macrophage physiological function after superparamagnetic iron oxide labeling
J. Hsiao (2008)
10.1016/J.COLSURFB.2006.12.019
Iron hydroxide nanoparticles coated with poly(ethylene glycol)-poly(aspartic acid) block copolymer as novel magnetic resonance contrast agents for in vivo cancer imaging.
M. Kumagai (2007)
Macrophages loaded with gold nanoshells for photothermal ablation of glioma: An in vitro model
A. R. Makkouk (2010)
10.1016/J.CLAY.2010.03.001
Toxicity and immunological activity of silver nanoparticles
S. Daniel (2010)
10.1016/j.tox.2010.06.002
Inflammatory responses may be induced by a single intratracheal instillation of iron nanoparticles in mice.
E. Park (2010)
Imaging of Neuro-Inflammation
T. Tourdias (2012)
10.3990/1.9789036537018
Detection of magnetic nanoparticles for clinical interventions
M. Visscher (2014)
10.1016/J.NANTOD.2011.10.001
Toxicology and clinical potential of nanoparticles
L. Yildirimer (2011)
Iron Nanoparticles as Magnetic Resonance Imaging Contrast Agents
P. Ferguson (2011)
10.1002/9783527610419.NTLS0143
Toxicity of Spherical and Anisotropic Nanosilica
Yuhui Jin (2010)
10.3727/096368911X593118
Cationic Gd-DTPA Liposomes for Highly Efficient Labeling of Mesenchymal Stem Cells and Cell Tracking with MRI
Jamal Guenoun (2012)
10.1007/978-3-662-56333-5_8
MRI and Ultrasound Imaging of Nanoparticles for Medical Diagnosis
O. Perlman (2018)
10.1002/9781119242666.CH5
Nanostructured Ceramics and Bioceramics for Bone Cancer Treatment
B. Palazzo (2016)
10.1080/17435390.2019.1698779
Mechanisms for cellular uptake of nanosized clinical MRI contrast agents
Emily J Guggenheim (2020)
Send Orders for Reprints to Reprints@benthamscience.net Recent Advances in Superparamagnetic Iron Oxide Nanoparticles for Cellular Imag- Ing and Targeted Therapy Research
Yi-Xiang J Wang ()
10.1080/00222930701314932
Cellular responses to nanoparticles: Target structures and mechanisms
K. Unfried (2007)
10.5482/HAMO-14-11-0061
MRI, the technology for imaging of thrombi and inflammation.
C. von zur Muhlen (2015)
Ef fi cient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells
A. Kunzmann (2011)
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