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Optical Biosensors For Immunoassays: The Fluorescence Capillary-fill Device.

R. Badley, R. Drake, I. Shanks, A. Smith, P. Stephenson
Published 1987 · Biology, Medicine

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This paper reports, for the first time, details of a novel type of optical biosensor for immunoassays, the fluorescence capillary-fill device (FCFD). This is based on a straightforward adaptation of the technology used to mass manufacture liquid-crystal display (LCD) cells to give cheap disposable immunosensors. These merely require contact by the sample to give a result in about a minute, and use certain principles of optical fibres and waveguides to avoid the need for operator attention, for physical separation methods or for washing steps. After a very brief introductory review and classification of optical biosensors, the main features of the FCFD and its associated instrumentation are described. The optical characteristics of the FCFD are then described, followed by accounts of the immunoassay method, the measurement system used in the experiments, the fabrication of FCFD sensors and a detailed description of the design of a competitive immunoassay for human immunoglobulin G (hIgG). The experimental details and the results of a first attempt at such an assay are then presented and discussed. It is concluded that the demonstration of this assay is a significant achievement, because the format of the FCFD, its manufacturing process and its instrumentation are completely novel. Certain problem areas have been identified and quantified; intended further work on these is outlined.
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This paper is referenced by
10.1016/0956-5663(93)80072-W
Optical immunosensing systems — are they meeting market needs?
G. A. Robinson (1993)
10.1016/0925-4005(92)80354-Z
Use of a fibre optic immunosensor for the detection of pesticides
F. Bier (1992)
10.1016/0040-6090(88)90090-9
Surface acoustic wave sensors incorporating Langmuir-Blodgett films
B. Holcroft (1988)
10.1007/BF02524233
Biosensors: a viable monitoring technology?
S. P. Higson (2006)
10.1016/0925-4005(95)01666-X
Use of an optical biosensor to measure prostate-specific antigen in whole blood
P. O'Neill (1995)
10.1016/0377-0427(95)00041-0
Product integration methods for solving a system of nonlinear Volterra integral equations
B. Jumarhon (1996)
10.1007/978-1-4615-4181-3_6
Nonseparation Electrochemical Enzyme Immunoassay Using Microporous Gold Electrodes
M. Ducey (2000)
10.1016/0250-6874(89)80030-7
Current trends in biosensor research and development
A. Turner (1988)
10.1016/S0009-8981(03)00241-9
Biosensors in clinical chemistry.
P. D'Orazio (2003)
10.1016/0019-0578(92)90015-B
Instrumentation control and automation in the control of biological effluent treatment.
Rick Briggs (1992)
10.1016/0956-5663(94)80038-3
Biochemical aspects of biosensors.
M. Byfield (1994)
10.21427/D7BS3C
Mathematical Methods for Biosensor Models
Q. Wang (2011)
10.1093/CLINCHEM/42.2.193
Immunosensors: technology and opportunities in laboratory medicine.
C. L. Morgan (1996)
Biosensor-based studies on coumarins
G. Keating (1998)
10.1016/0167-7012(88)90039-5
Analytical applications of immobilised proteins and cells
M. Coughlan (1988)
10.1016/S0923-2532(05)80165-6
Immunocapteurs et biocapteurs non enzymatiques: principes et applications possibles en analyse
P. Binder (1990)
10.1016/0956-5663(91)80003-G
Optical immunosensing systems--meeting the market needs.
G. A. Robinson (1991)
10.1016/S0140-6736(94)91151-7
Prostate specific antigen estimation with optical biosensor
S. Hampson (1994)
Antigen-antibody interactions measurements using surface plasmon fluorescence spectroscopy
Margarida M L M Vareiro (2006)
10.1016/0956-5663(91)80005-I
Sensitivity enhancement of optical immunosensors by the use of a surface plasmon resonance fluoroimmunoassay.
J. Attridge (1991)
10.1007/978-3-642-78660-0_10
Die Entwicklung optischer Biosensoren
E. A. Hall (1995)
10.1007/BF00042754
A mathematical model of a biosensor
S. Jones (1996)
10.1366/000370204773580257
Ultrasensitive Detection of 1, 4-Bis(4-Vinylpyridyl)Phenylene in a Small Volume of Low Refractive Index Liquid by Surface-Enhanced Raman Scattering-Active Light Waveguide
W. Xu (2004)
10.1007/978-3-319-05365-3_31
Modelling a Competitive Antibody/Antigen Chemical Reaction that Occurs in the Fluorescence Capillary-Fill Device
Magda Rebelo (2014)
10.1177/002029409102400502
Instrumentation and Support Systems for Monitoring and Control of Source and Receiving Water Quality and Water and Wastewater Treatment Processes
R. Briggs (1991)
10.1093/CLINCHEM/36.8.1408
A decade of development in immunoassay methodology.
J. P. Gosling (1990)
10.1007/978-1-349-11234-0_18
Surface effect immunoassay
R. M. Sutherland (1991)
10.1016/0925-4005(94)87038-1
Calculation of the angular distribution and waveguide capture efficiency of the light emitted by a fluorophore situated at or adsorbed to the waveguide side wall
V. Ratner (1994)
10.1016/B978-044453125-4.50005-X
Planar Waveguides for Fluorescence Biosensors
K. Sapsford (2008)
10.1002/9781118992005.CH6
Stents, blood flow and pregnancy : mathematical modelling in the raw
Sean McKee (2015)
10.1088/0034-4885/60/11/005
Biosensors: recent advances
A. Collings (1997)
10.1007/0-306-47060-8_14
Principles of Fluorescence Immunoassay
A. Ozinskas (2002)
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