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Newcastle Disease Virus: The Effect Of Monoclonal Antibody In The Overlay On Virus Penetration And The Immunoselection Of Variants.

P. Russell
Published 1984 · Biology, Medicine

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Monoclonal antibody to the haemagglutinin-neuraminidase or the fusion glycoprotein of Newcastle disease virus blocked virus penetration which otherwise occurred within 90 s of the temperature being increased from 4 degrees C to 37 degrees C. These antibodies regularly immunoselected variant plaques only when incorporated into the agarose overlay. Variant viruses then formed plaques whereas the growth of non-neutralized normal virus was suppressed.
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