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Cloning Of Complete Genome Sets Of Six DsRNA Viruses Using An Improved Cloning Method For Large DsRNA Genes.

A. Potgieter, A. Steele, A. V. van Dijk
Published 2002 · Medicine, Biology

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Cloning full-length large (>3 kb) dsRNA genome segments from small amounts of dsRNA has thus far remained problematic. Here, a single-primer amplification sequence-independent dsRNA cloning procedure was perfected for large genes and tailored for routine use to clone complete genome sets or individual genes. Nine complete viral genome sets were amplified by PCR, namely those of two human rotaviruses, two African horsesickness viruses (AHSV), two equine encephalosis viruses (EEV), one bluetongue virus (BTV), one reovirus and bacteriophage Phi12. Of these amplified genomes, six complete genome sets were cloned for viruses with genes ranging in size from 0.8 to 6.8 kb. Rotavirus dsRNA was extracted directly from stool samples. Co-expressed EEV VP3 and VP7 assembled into core-like particles that have typical orbivirus capsomeres. This work presents the first EEV sequence data and establishes that EEV genes have the same conserved termini (5' GUU and UAC 3') and coding assignment as AHSV and BTV. To clone complete genome sets, one-tube reactions were developed for oligo-ligation, cDNA synthesis and PCR amplification. The method is simple and efficient compared to other methods. Complete genomes can be cloned from as little as 1 ng dsRNA and a considerably reduced number of PCR cycles (22-30 cycles compared to 30-35 of other methods). This progress with cloning large dsRNA genes is important for recombinant vaccine development and determination of the role of terminal sequences for replication and gene expression.
This paper references
10.1016/0734-9750(93)90407-E
Development of recombinant vaccines against bluetongue.
A. A. V. Dijk (1993)
Characterization of phi12, a bacteriophage related to phi6: nucleotide sequence of the large double-stranded RNA.
P. Gottlieb (2002)
10.1128/JVI.73.6.4813-4822.1999
Heterotypic Protection and Induction of a Broad Heterotypic Neutralization Response by Rotavirus-Like Particles
S. Crawford (1999)
10.1006/VIRO.2002.1436
Characterization of φ12, a Bacteriophage Related to φ6: Nucleotide Sequence of the Large Double-Stranded RNA
P. Gottlieb (2002)
The protective efficacy of a recombinant VP 2 - basedAfrican horsesickness subunit
M. Scanlen (2002)
10.1016/S0140-6736(99)00129-4
Low-cost drug cuts perinatal HIV-transmission rate
M. Mccarthy (1999)
Characterization of phi 12, a bacteriophage related to phi 6: nucleotide sequence of the small and middle double-stranded RNA.
P. Gottlieb (2002)
African horsesickness vaccine development
A A Van Dijk (1999)
The 3h- terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5h-terminus
Z K Xu (1989)
10.1128/JVI.65.12.6994-6997.1991
Assembly of double-shelled rotaviruslike particles by simultaneous expression of recombinant VP6 and VP7 proteins.
M. Sabara (1991)
10.1016/0042-6822(92)90176-P
Active protection against rotavirus infection of mice following intraperitoneal immunization.
M. Mcneal (1992)
10.1128/JB.181.15.4505-4508.1999
Isolation of additional bacteriophages with genomes of segmented double-stranded RNA.
L. Mindich (1999)
10.1016/S0264-410X(01)00445-5
The protective efficacy of a recombinant VP2-based African horsesickness subunit vaccine candidate is determined by adjuvant.
M. Scanlen (2002)
10.1006/VIRO.1996.0304
Immunization with VP2 is sufficient for protection against lethal challenge with African horsesickness virus Type 4.
M. Stone-Marschat (1996)
African horsesickness vaccine development
A. A. van Dijk (1999)
10.1016/S0264-410X(98)00319-3
Biochemical and immunologic comparison of virus-like particles for a rotavirus subunit vaccine.
H. P. Madore (1999)
10.1016/S0166-0934(98)00031-7
Sequence-independent amplification and cloning of large dsRNA virus genome segments by poly(dA)-oligonucleotide ligation.
F. Vreede (1998)
10.1099/0022-1317-77-6-1211
Full protection against African horsesickness (AHS) in horses induced by baculovirus-derived AHS virus serotype 4 VP2, VP5 and VP7.
J. Martínez-Torrecuadrada (1996)
10.1016/S0168-1702(01)00442-7
Human group C rotavirus: completion of the genome sequence and gene coding assignments of a non-cultivatable rotavirus.
Zhilin Chen (2002)
candidate is determined by adjuvant
M. A. Stone-Marschat (1996)
10.1099/0022-1317-79-10-2481
Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus.
H. Attoui (1998)
Protein aggregation complicates the development of baculovirus-expressed African horsesickness virus serotype 5 VP2 subunit vaccines.
M. du Plessis (1998)
10.1099/0022-1317-79-2-333
Intracellular production of African horsesickness virus core-like particles by expression of the two major core proteins, VP3 and VP7, in insect cells.
S. Maree (1998)
CCCC Complete genome set cloning for dsRNA viruses Complete genome set cloning for dsRNA viruses
10.1016/S0166-0934(99)00126-3
A strategy for rapid cDNA cloning from double-stranded RNA templates isolated from plants infected with RNA viruses by using Taq DNA polymerase.
Y. Zhang (2000)
10.1073/PNAS.79.24.7644
Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene.
L. Cashdollar (1982)
Characterization of bacteriophage phi12, a bacteriophage related to phi6 : nucleotide sequence of the large double-stranded RNA
10.1016/0042-6822(84)90438-0
Molecular cloning of the complete genome of reovirus serotype 3.
L. Cashdollar (1984)
10.1177/000608446801900413
Received
(1968)
10.1099/0022-1317-81-6-1507
Complete sequence determination and genetic analysis of Banna virus and Kadipiro virus: proposal for assignment to a new genus (Seadornavirus) within the family Reoviridae.
H. Attoui (2000)
10.1006/VIRO.2001.1288
Characterization of φ12, a Bacteriophage Related to φ6: Nucleotide Sequence of the Small and Middle Double-Stranded RNA
P. Gottlieb (2002)
10.1016/0042-6822(91)90452-H
Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus.
S. Cooke (1991)
10.1016/0166-0934(94)90123-6
Baculovirus expression of non-structural protein NS2 and core protein VP7 of African horsesickness virus serotype 3 and their use as antigens in an indirect ELISA.
C. Bremer (1994)
10.1007/978-3-7091-6607-9
Virus Taxonomy
G. Martelli (1995)
10.1073/PNAS.84.23.8301
Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs.
J. Anzola (1987)
10.1128/JVI.68.9.5945-5952.1994
Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells.
S. Crawford (1994)
10.1016/0042-6822(90)90557-8
Molecular cloning and comparative sequence analyses of bluetongue virus S1 segments by selective synthesis of specific full-length DNA copies of dsRNA genes.
T. Kowalik (1990)
10.1016/S0140-6736(05)75216-8
Rotavirus vaccine put on hold in USA after intussusception reports
M. Mccarthy (1999)
10.1016/0264-410X(94)90289-5
Long-lasting protection of sheep against bluetongue challenge after vaccination with virus-like particles: evidence for homologous and partial heterologous protection.
P. Roy (1994)
10.1016/S1366-2120(08)70117-X
A T vector with very low background levels
T. Franz (1998)
Characterization of phi12, a bacteriophage related to phi6 : nucleotide sequence of the small and middle double-stranded RNA
10.1099/0022-1317-78-11-2895
Complete nucleotide sequence of Colorado tick fever virus segments M6, S1 and S2.
H. Attoui (1997)
10.1016/S0166-0934(00)00191-9
Development of probes for typing African horsesickness virus isolates using a complete set of cloned VP2-genes.
J. J. Koekemoer (2000)
10.1128/JVI.64.5.1998-2003.1990
Recombinant virus vaccine for bluetongue disease in sheep.
P. Roy (1990)
10.1099/0022-1317-70-8-2007
The characterization of equine encephalosis virus and the development of genomic probes.
G. Viljoen (1989)
10.1099/0022-1317-81-3-831
Identification and differentiation of the nine African horse sickness virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2.
C. Sailleau (2000)
10.1099/0022-1317-80-12-3181
Molecular characterization of human group C rotavirus genes 6, 7 and 9.
V. James (1999)
10.1016/0042-6822(89)90443-1
The 3'-terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5'-terminus.
Z. Xu (1989)
10.1099/0022-1317-77-9-2053
Recombinant baculovirus-synthesized African horsesickness virus (AHSV) outer-capsid protein VP2 provides protection against virulent AHSV challenge.
P. Roy (1996)
10.1016/S0166-0934(00)00212-3
Strategies for the sequence determination of viral dsRNA genomes.
H. Attoui (2000)
10.1128/JVI.64.8.3635-3642.1990
Polypeptide composition of rotavirus empty capsids and their possible use as a subunit vaccine.
H. Brüssow (1990)
10.1128/JVI.66.3.1817-1822.1992
Cloning of noncultivatable human rotavirus by single primer amplification.
P. Lambden (1992)
10.1006/VIRO.1995.1322
The genome segments of DpRV, a commensal reovirus of the wasp Diadromus pulchellus (Hymenoptera).
Y. Bigot (1995)



This paper is referenced by
10.1099/vir.0.036426-0
Genetic divergence and classification of non-structural protein 1 among porcine rotaviruses of species B.
T. Suzuki (2011)
Development and evaluation of a real-time polymerase chain reaction assay for equine encephalosis virus
N M Rathogwa (2012)
10.1016/J.JVIROMET.2004.11.006
The use of reverse transcriptase for efficient first- and second-strand cDNA synthesis from single- and double-stranded RNA templates.
I. Tzanetakis (2005)
10.1080/00359190809519208
A century of research achievements by the Onderstepoort Veterinary Institute
R. Bigalke (2008)
10.1007/s00705-017-3320-6
Characterization of a novel strain of Botryosphaeria dothidea chrysovirus 1 from the apple white rot pathogen Botryosphaeria dothidea
Zheng Ding (2017)
10.1007/s00705-010-0852-4
Nucleotide sequences of four RNA segments of a reovirus isolated from the mud crab Scylla serrata provide evidence that this virus belongs to a new genus in the family Reoviridae
Jigang Chen (2010)
10.1111/J.1439-0450.2005.00883.X
VP2-segment sequence analysis of some isolates of bluetongue virus recovered in the Mediterranean basin during the 1998-2003 outbreak.
A. Potgieter (2005)
The characterization of inner core protein VP6 of African horsesickness virus
De Waal (2007)
10.1016/j.vetmic.2012.07.007
Bluetongue virus genetic and phenotypic diversity: towards identifying the molecular determinants that influence virulence and transmission potential.
P. Coetzee (2012)
10.1007/s00705-005-0685-8
Complete nucleotide sequences and genome characterization of double-stranded RNA 1 and RNA 2 in the Raphanus sativus-root cv. Yipinghong
L. Chen (2005)
10.1016/J.JVIROMET.2007.02.016
Rapid cDNA synthesis and sequencing techniques for the genetic study of bluetongue and other dsRNA viruses.
S. Maan (2007)
10.1007/S11434-008-0467-2
Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae
Guohui Zhou (2008)
Comparative Analysis of Cloned Viral Double-Stranded (ds) RNA From Rhizoctonia solani Anastomosis Group 13 Isolates 357, 303, & 386
Brandon Alan Michalides (2019)
10.1016/j.virol.2010.05.015
Plant-feeding insects harbor double-stranded RNA viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses.
Allyn R. Spear (2010)
10.1007/978-3-319-47426-7_1
Bluetongue: Aetiology, Epidemiology, Pathogenesis, Diagnosis and Control
P. P. Rao (2017)
10.1099/vir.0.057984-0
Genome sequencing identifies genetic and antigenic divergence of porcine picobirnaviruses.
K. Bányai (2014)
10.1016/j.virol.2009.01.037
A new Phytoreovirus infecting the glassy-winged sharpshooter (Homalodisca vitripennis).
D. Stenger (2009)
10.1016/j.virusres.2016.04.007
African horse sickness virus serotype 4 antigens, VP1-1, VP2-2, VP4, VP7 and NS3, induce cytotoxic T cell responses in vitro.
F E Faber (2016)
10.3390/V8100268
Molecular Characterization of a Trisegmented Mycovirus from the Plant Pathogenic Fungus Colletotrichum gloeosporioides
Jie Zhong (2016)
10.1128/JVI.80.1.460-473.2006
Nonstructural Protein 3 of Bluetongue Virus Assists Virus Release by Recruiting ESCRT-I Protein Tsg101
C. Wirblich (2006)
10.1111/JPH.12569
Sequence‐independent Amplification with Genome Multiplexing to Establish Complete Genome of Multipartite RNA Viruses: Cucumber mosaic virus as a Case Study
Surender Kumar (2017)
10.1099/VIR.0.18919-0
A first full outer capsid protein sequence data-set in the Orbivirus genus (family Reoviridae): cloning, sequencing, expression and analysis of a complete set of full-length outer capsid VP2 genes of the nine African horsesickness virus serotypes.
A. Potgieter (2003)
10.1016/J.JEVS.2008.01.017
First Report of an Outbreak of African Horsesickness Virus Serotype 2 in the Northern Hemisphere
F. Fasina (2008)
10.1099/vir.0.2008/001933-0
Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses.
Shengjun Jiang (2008)
10.1016/j.virusres.2014.05.028
Detection and characterization of a novel Gammapartitivirus in the phytopathogenic fungus Colletotrichum acutatum strain HNZJ001.
J. Zhong (2014)
10.1016/j.jviromet.2010.08.013
Optimized approaches for the sequence determination of double-stranded RNA templates.
O. Darissa (2010)
Cloning viral dsRNA genomes : analysis and application
A. Potgieter (2004)
The use of reverse transcriptase for efficient first-and second-strand cDNA synthesis from single-and double-stranded RNA templates
I. E. Tzanetakisa (2005)
A Novel and Sensitive Molecular Method for Nucleic Acid Discovery in CSF Samples
Sana A Alshaikh (2011)
10.1016/j.vaccine.2008.07.037
Evaluation of the pathogenicity of African Horsesickness (AHS) isolates in vaccinated animals.
B. V. von Teichman (2008)
10.1016/bs.aivir.2017.08.003
Bluetongue Virus: From BTV-1 to BTV-27.
G. Belbis (2017)
10.1016/J.JVIROMET.2005.04.015
Serogroup-reactive and type-specific detection of bluetongue virus antibodies using chicken scFvs in inhibition ELISAs.
J. Fehrsen (2005)
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