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Transcriptional Regulation Of The Mercury-resistance Genes Of Transposon Tn501.

P. Lund, S. J. Ford, N. Brown
Published 1986 · Biology, Medicine

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Expression of the mercury-resistance (mer) genes of the transposon Tn501 is positively and negatively controlled by the product of the merR gene. DNA sequence analysis has identified three open reading frames as potential candidates for this gene, one of which is oriented divergently with respect to the mercury-resistance genes. We have demonstrated that although RNA polymerase will bind to fragments containing the potential control regions for all three reading frames, only the control region for this divergent reading frame shows detectable promoter activity in vivo. Transcription of this reading frame is required for repression and induction of mer transcription. We have also shown that the Tn501 merR gene product negatively regulates its own synthesis, and have identified the start point of the transcript for this reading frame and for the mercury-inducible transcript of the mercury-resistance genes.
This paper references
10.1073/pnas.74.12.5463
DNA sequencing with chain-terminating inhibitors.
F. Sanger (1977)
10.1021/BI00286A015
Nucleotide sequence of a gene from the Pseudomonas transposon Tn501 encoding mercuric reductase.
N. Brown (1983)
10.1128/JB.134.3.1141-1156.1978
Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
A. C. Chang (1978)
10.1128/MMBR.48.2.95-124.1984
Mechanisms of microbial resistance and detoxification of mercury and organomercury compounds: physiological, biochemical, and genetic analyses.
J. B. Robinson (1984)
10.1146/ANNUREV.BI.53.070184.001453
Protein-DNA recognition.
C. Pabo (1984)
10.1021/BI00692A011
A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
R. Burgess (1975)
10.1146/ANNUREV.MI.32.100178.003225
Microbial transformations of metals.
A. Summers (1978)
10.1107/S0108767381098942
Structure of catabolite gene activator protein at 2.9 Ã resolution suggests binding to left handed B-DNA
T. Steitz (1981)
RNA polymerase binding sites in lambdaplac5 DNA.
B. Jones (1977)
Organisation of transcriptional signals in plasmids pBR 322 and pACYCl 84
D. STUBER (1981)
10.1073/PNAS.80.1.21
The tac promoter: a functional hybrid derived from the trp and lac promoters.
H. D. de Boer (1983)
10.1128/JB.155.2.593-600.1983
In vivo regulation of the Escherichia coli araC promoter.
S. Hahn (1983)
Molecular genetic analysis of the merucric ion resistance ( mer ) operon of plasmid R 100
N. N. NI’BHRIAIN (1983)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1016/0378-1119(81)90182-7
Cloning a promoter that puts the expression of tetracycline resistance under the control of the regulatory elements of the mer operon.
F. Bohlander (1981)
A multi - purpose cloning system based on the single - stranded bacteriophage M 13
J. MESSING (1979)
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
R. Burgess (1969)
10.1128/JB.140.1.167-181.1979
Transposon A-generated mutations in the mercuric resistance genes of plasmid R100-1.
T. Foster (1979)
10.1038/290754A0
Structure of the cro repressor from bacteriophage λ and its interaction with DNA
W. Anderson (1981)
10.1073/PNAS.66.3.773
Catabolite-insensitive revertants of lac promoter mutants.
A. Silverstone (1970)
Sequence of mercuric reductase structural
T. K. MISRA (1985)
10.1038/282864A0
Nucleotide sequence analysis of the chloramphenicol resistance transposon Tn9
N. K. Alton (1979)
Quantum chemistry and protein folding : the art of the possible
A. D. MCLACHLAN (1977)
10.1038/298443A0
The operator-binding domain of λ repressor: structure and DNA recognition
C. Pabo (1982)
10.1128/JB.155.2.690-703.1983
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100.
N. Nibhriain (1983)
10.1016/0378-1119(79)90082-9
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
M. Dagert (1979)
10.1128/JB.97.1.376-385.1969
Composite circular forms of R factor deoxyribonucleic acid molecules.
T. Nisioka (1969)
10.1016/S0022-2836(83)80022-9
Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. III. Nucleotide sequence and regulation of the lysR gene.
P. Stragier (1983)
Experiments in molecular genetics
J. Miller (1972)
10.1021/BI00261A022
Nitrocellulose filter binding studies of the interactions of Escherichia coli RNA polymerase holoenzyme with deoxyribonucleic acid restriction fragments: evidence for multiple classes of nonpromoter interactions, some of which display promoter-like properties.
P. Melançon (1982)
The DNA sequence of the mercury resistance operon of the IncFII plasmid NR1.
P. Barrineau (1984)
10.1073/PNAS.62.4.1159
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
D. Clewell (1969)
10.1073/PNAS.77.6.3346
The Escherichia coli L-arabinose operon: binding sites of the regulatory proteins and a mechanism of positive and negative regulation.
S. Ogden (1980)
10.1016/S0076-6879(80)65059-9
Sequencing end-labeled DNA with base-specific chemical cleavages.
A. Maxam (1980)
Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors.
C. W. Adams (1984)
Structure of the cro repressor from bacteriophage lambda and its interaction with DNA.
W. Anderson (1981)
10.1016/0092-8674(77)90272-0
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
A. Berk (1977)
10.1016/0968-0004(85)90069-6
Bacterial resistance to mercury — reductio ad absurdum?
N. Brown (1985)
Positive control of transcription in bacteria
J. B. - ROBINSON (1984)
10.1073/PNAS.71.9.3635
Direction of transcription of the regulatory gene araC in Escherichia coli B-r.
G. Wilcox (1974)
10.1093/nar/9.13.2989
Rapid and efficient cosmid cloning
D. Ish-Horowicz (1981)
from plasmid R 100 and transposon Tn 5 OI : functional domains of the enzyme
N. N. NI’BHRIAIN (1985)
10.1128/JB.162.2.773-776.1985
Some mercurial resistance plasmids from different incompatibility groups specify merR regulatory functions that both repress and induce the mer operon of plasmid R100.
T. Foster (1985)
10.1128/JB.162.1.441-444.1985
Use of transcriptional repressors to stabilize plasmid copy number of transcriptional fusion vectors.
P. F. Lambert (1985)
Anti - mRNA : specific inhibition of translation of mRN A molecules
M. SCHWARZ (1984)
10.1093/NAR/11.8.2237
Compilation and analysis of Escherichia coli promoter DNA sequences.
D. Hawley (1983)
The operator-binding domain of lambda repressor: structure and DNA recognition.
C. Pabo (1982)
Mercuric ion-resistance operons of plasmid R 100 and transposon Tn 5 Ol : The beginning of the operon including the regulatory region and the first two structural genes ( heavy metal resistance / bacterial plasmids / DNA sequencing )
T. Misra
10.1128/JB.163.3.1153-1157.1985
Identification of the merR gene of R100 by using mer-lac gene and operon fusions.
T. Foster (1985)
10.1128/JB.153.2.872-877.1983
Identification and control of synthesis of the dsdC activator protein.
E. McFall (1983)
10.1128/JB.149.2.479-487.1982
Polypeptides encoded by the mer operon.
W. Jackson (1982)



This paper is referenced by
Characterization of Pseudomonas Mercury-resistance
()
10.1007/4735_104
Family matters: gene regulation by metal-dependent transcription factors
H. V. Bakel (2005)
10.1111/j.1365-2958.1991.tb01979.x
The merR regulatory gene in Thiobacillus ferrooxidans is spaced apart from the mer structural genes
C. Inoue (1991)
10.1007/978-3-642-60691-5
Mechanisms of Transcription
F. Eckstein (1997)
10.1128/JB.183.19.5651-5658.2001
Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34.
B. Borremans (2001)
10.1021/BI00472A001
DNA distortion accompanies transcriptional activation by the metal-responsive gene-regulatory protein MerR.
B. Frantz (1990)
10.1016/S0168-6445(03)00041-X
Zn(II) metabolism in prokaryotes.
Dayle K Blencowe (2003)
10.1016/0378-1119(87)90306-4
Two promoters from the Streptomyces plasmid pIJ101 and their expression in Escherichia coli.
M. Buttner (1987)
10.1128/JB.178.9.2712-2714.1996
Intracellular inducer Hg2+ concentration is rate determining for the expression of the mercury-resistance operon in cells.
H. Yu (1996)
10.1021/BI00441A002
Mutagenesis of the cysteines in the metalloregulatory protein MerR indicates that a metal-bridged dimer activates transcription.
L. Shewchuk (1989)
10.1016/S0168-6445(03)00051-2
The MerR family of transcriptional regulators.
N. Brown (2003)
Homéostasie et résistance au cuivre chez Cupriavidusmetallidurans CH34 : la proteine CopH et les transporteurs membranaires CuSa et CzcA
V. Sendra (2007)
10.17582/journal.pjz/20190426100430
Molecular Characterization of Mercury Resistant Bacteria Isolated from Tannery Wastewater
A. Amin (2020)
APPLICAZIONE DI INDICATORI FISICI, CHIMICI E BIOLOGICI PER VALUTARE LA QUALITA' E LO STATO DI SALUTE DEI SUOLI. IL CASO DELLA REGIONE LOMBARDIA
Laura Guidotti (2015)
10.1006/JMBI.1998.2060
Complete sequence of the IncPbeta plasmid R751: implications for evolution and organisation of the IncP backbone.
P. Thorsted (1998)
10.1016/S0162-0134(99)00234-2
Bacterial metal-resistance proteins and their use in biosensors for the detection of bioavailable heavy metals.
I. Bontidean (2000)
10.1002/JCB.240460204
Bacterial resistances to mercury and copper
Andrew P. Morby (1991)
10.1128/AEM.60.11.4059-4065.1994
merA gene expression in aquatic environments measured by mRNA production and Hg(II) volatilization.
S. Nazaret (1994)
10.1074/jbc.272.47.29518
A Mercuric Ion Uptake Role for the Integral Inner Membrane Protein, MerC, Involved in Bacterial Mercuric Ion Resistance*
L. Sahlman (1997)
10.1016/S0006-291X(03)00249-3
ZccR--a MerR-like regulator from Bordetella pertussis which responds to zinc, cadmium, and cobalt.
S. Kidd (2003)
10.1146/ANNUREV.MI.42.100188.003441
Plasmid-mediated heavy metal resistances.
S. Silver (1988)
"Prokaryotic Metallothionein gene isolation, Nucleotide sequence and expression"
J. Huckle (1993)
10.1016/0147-619X(92)90002-R
Bacterial resistances to inorganic mercury salts and organomercurials.
T. Misra (1992)
Isolation, Identification and biochemical analysis of Mercury Resistant Bacteria (MRB) from the effluent water of Rourkela Steel Plant, Orissa
Sony Priyadarshini (2011)
10.1111/j.1574-6941.2012.01460.x
The mercury resistance (mer) operon in a marine gliding flavobacterium, Tenacibaculum discolor 9A5.
Rachel Allen (2013)
10.1016/S0168-6445(03)00045-7
Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes.
M. Mergeay (2003)
10.1111/j.1365-2958.1995.mmi_17010025.x
The role of cysteine residues in the transport of mercuric ions by the Tn501 MerT and MerP mercury‐resistance proteins
A. Morby (1995)
10.3109/10408418709104455
The genetics and biochemistry of mercury resistance.
T. Foster (1987)
DNA-Protein interaction: a response regulatory protein associated with Mo homeostasis in Desulfovibrio alaskensis G20.
Nathália Santos de Castro (2014)
10.1007/978-3-642-60691-5_9
Indirect Effects of DNA Sequence on Transcriptional Activation by Prokaryotic DNA Binding Proteins
S. Hilchey (1997)
10.1002/(SICI)1097-0134(19991115)37:3<429::AID-PROT10>3.0.CO;2-P
Unusual amino acid usage in the variable regions of mercury‐binding antibodies
C. M. Westhoff (1999)
10.1046/j.1365-2958.1999.01229.x
ZntR is a Zn(II)‐responsive MerR‐like transcriptional regulator of zntA in Escherichia coli
K. R. Brocklehurst (1999)
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