Online citations, reference lists, and bibliographies.
← Back to Search

The 120 592 Bp IncF Plasmid PRSB107 Isolated From A Sewage-treatment Plant Encodes Nine Different Antibiotic-resistance Determinants, Two Iron-acquisition Systems And Other Putative Virulence-associated Functions.

R. Szczepanowski, S. Braun, V. Riedel, S. Schneiker, I. Krahn, A. Pühler, A. Schlüter
Published 2005 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
The antibiotic-multiresistance IncF plasmid pRSB107 was isolated by a transformation-based approach from activated-sludge bacteria of a wastewater-treatment plant. It confers resistance to ampicillin, penicillin G, chloramphenicol, erythromycin, kanamycin, neomycin, streptomycin, sulfonamides, tetracycline and trimethoprim and against mercuric ions. Complete sequencing of this plasmid revealed that it is 120 592 bp in size and has a G+C content of 53.1 mol%. The plasmid backbone is composed of three replicons, RepFIA, RepFIB and RepFII, which are almost identical to corresponding regions located on the F-plasmid and on R100. The three replicons encode replication initiation (rep) and replication control, multimer resolution (mrs), post-segregational killing of plasmid-free cells (psk) and active plasmid partitioning (sopABC locus). Part of the F-leading region and remnants of the F-homologous DNA-transfer (tra) module complete the pRSB107 backbone. Plasmid pRSB107 contains a complex, highly mosaic 35 991 bp antibiotic-resistance region consisting of a Tn21- and a Tn10-derivative and a chloramphenicol-resistance module. The Tn21 derivative is composed of a mercury-resistance region (mer), a Tn4352B-like kanamycin/neomycin-resistance transposon, a streptomycin/sulfonamide-resistance module, remnants of the beta-lactam-resistance transposon Tn1, a macrolide-resistance module flanked by copies of IS26 and IS6100, remnants of Tn402 integrating a class 1 integron and the Tn21-specific transposition module. A truncated version of the tetracycline-resistance transposon Tn10 and the chloramphenicol acetyltransferase gene catA complete the pRSB107 resistance region. In addition to antibiotic resistance, pRSB107 encodes the following putative virulence-associated functions: (i) an aerobactin iron-acquisition siderophore system (iuc/iut); (ii) a putative high-affinity Fe(2+) uptake system which was previously identified on a pathogenicity island of Yersinia pestis and in the genome of the phytopathogen Erwinia carotovora subsp. atroseptica SCRI1043; (iii) an sn-glycerol-3-phosphate transport system (ugp); and (iv) the virulence-associated genes vagCD having a possible function in stable plasmid inheritance. All the accessory modules are framed by insertion sequences, indicating that pRSB107 was gradually assembled by integration of different horizontally acquired DNA segments via transposition or homologous recombination.
This paper references
10.1128/JB.167.1.350-355.1986
Characterization of iucA and iucC genes of the aerobactin system of plasmid ColV-K30 in Escherichia coli.
V. de Lorenzo (1986)
10.1128/IAI.69.5.3271-3285.2001
Complete DNA Sequence and Analysis of the Large Virulence Plasmid of Shigella flexneri
M. Venkatesan (2001)
10.1128/JB.172.12.6818-6825.1990
Comparative genetic organization of incompatibility group P degradative plasmids.
R. S. Burlage (1990)
10.1111/J.1574-6968.1997.TB10299.X
A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains.
H. Schmidt (1997)
10.1128/IAI.55.9.1963-1969.1987
Evaluation with an iuc::Tn10 mutant of the role of aerobactin production in the virulence of Shigella flexneri.
X. Nassif (1987)
10.1146/ANNUREV.MICRO.53.1.43
Addiction modules and programmed cell death and antideath in bacterial cultures.
H. Engelberg-Kulka (1999)
10.1128/AAC.47.9.2732-2739.2003
Molecular Analysis of incHI1 Antimicrobial Resistance Plasmids from Salmonella Serovar Typhi Strains Associated with Typhoid Fever
J. Wain (2003)
10.1128/JB.169.5.1836-1846.1987
Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI plasmids P307 and F, and its relation to the RepA replicon of IncFII plasmids.
S. Saadi (1987)
10.1128/IAI.68.4.2096-2101.2000
Identification of Regions of the Escherichia coliChromosome Specific for Neonatal Meningitis-Associated Strains
S. Bonacorsi (2000)
10.1126/SCIENCE.8153624
Inactivation of antibiotics and the dissemination of resistance genes.
J. Davies (1994)
10.1006/PLAS.1999.1390
Nucleotide sequence of the F plasmid leading region.
N. Manwaring (1999)
Experiments in molecular genetics
J. Miller (1972)
10.1006/PLAS.1999.1421
Genetic exchange between bacteria in the environment.
J. Davison (1999)
10.1128/AEM.70.1.404-412.2004
Growth of Escherichia coli Coexpressing Phosphotriesterase and Glycerophosphodiester Phosphodiesterase, Using Paraoxon as the Sole Phosphorus Source
S. Y. McLoughlin (2004)
10.1128/JB.165.2.570-578.1986
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12.
V. de Lorenzo (1986)
10.1093/emboj/18.13.3856
Regulation of DNA replication by iterons: an interaction between the ori2 and incC regions mediated by RepE‐bound iterons inhibits DNA replication of mini‐F plasmid in Escherichia coli
H. Uga (1999)
10.1038/NBT1183-784
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria
R. Simon (1983)
10.1111/j.1574-6941.2002.tb01006.x
The prevalence and diversity of mobile genetic elements in bacterial communities of different environmental habitats: insights gained from different methodological approaches.
K. Smalla (2002)
Mosaic structure of plasmids from natural populations of Escherichia coli.
E. F. Boyd (1996)
10.1016/J.PLASMID.2004.09.004
Sequence of the 68,869 bp IncP-1alpha plasmid pTB11 from a waste-water treatment plant reveals a highly conserved backbone, a Tn402-like integron and other transposable elements.
Thomas Tennstedt (2005)
10.1093/NAR/26.18.4196
The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7.
V. Burland (1998)
Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.
K. H. Hu (1996)
10.1016/0378-1119(91)90482-Q
Identification of eleven single-strand initiation sequences (ssi) for priming of DNA replication in the F, R6K, R100 and ColE2 plasmids.
Nomura Nobuo (1991)
Molecular Cloning: a Labolatory Manual
J. Sambrook (1989)
10.1006/JMBI.1994.0078
The crystal structure of dihydrodipicolinate synthase from Escherichia coli at 2.5 A resolution.
C. Mirwaldt (1995)
10.1128/JB.170.1.56-64.1988
Nucleotide sequence of the iucD gene of the pColV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions.
M. Herrero (1988)
10.1074/JBC.M400021200
Reconstitution of F Factor DNA Replication in Vitro with Purified Proteins*
S. Zzaman (2004)
10.1099/00221287-8-1-89
An infective factor controlling sex compatibility in Bacterium coli.
L. L. Cavalli (1953)
10.1128/JB.175.13.4016-4024.1993
Regulatory interactions between RepA, an essential replication protein, and the DNA repeats of RepFIB from plasmid P307.
A. J. Spiers (1993)
10.1007/s004380050294
The localized melting of mini-F origin by the combined action of the mini-F initiator protein (RepE) and HU and DnaA of Escherichia coli
Y. Kawasaki (1996)
10.1128/IAI.71.5.2775-2786.2003
Complete Genome Sequence and Comparative Genomics of Shigella flexneri Serotype 2a Strain 2457T
J. Wei (2003)
10.1128/MMBR.63.3.507-522.1999
Transposon Tn21, Flagship of the Floating Genome
C. Liebert (1999)
10.1007/s004380051191
Phenotypic and molecular characterization of conjugative antibiotic resistance plasmids isolated from bacterial communities of activated sludge
M. Droege (2000)
10.1128/JB.185.19.5822-5830.2003
Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775.
M. Di Lorenzo (2003)
leading region
B. Martinez (2001)
10.1016/J.GENE.2004.05.008
Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG43.
Ying-Tsong Chen (2004)
10.1128/AAC.47.1.342-349.2003
In34, a Complex In5 Family Class 1 Integron Containing orf513 and dfrA10
S. Partridge (2003)
10.1016/S0378-1097(03)00430-0
F factor conjugation is a true type IV secretion system.
T. Lawley (2003)
10.1163/2210-7975_hrd-2952-2016002
The Development of
H. R. Robinson (1965)
10.1128/IAI.35.3.852-860.1982
Involvement of a plasmid in the invasive ability of Shigella flexneri.
P. Sansonetti (1982)
10.1073/PNAS.87.12.4645
Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
S. G. Grant (1990)
10.1099/MIC.0.27304-0
The complete sequences of plasmids pB2 and pB3 provide evidence for a recent ancestor of the IncP-1beta group without any accessory genes.
H. Heuer (2004)
10.1128/IAI.69.10.6012-6021.2001
Ferric Dicitrate Transport System (Fec) of Shigella flexneri 2a YSH6000 Is Encoded on a Novel Pathogenicity Island Carrying Multiple Antibiotic Resistance Genes
S. N. Luck (2001)
10.1093/NAR/29.22.4633
REPuter: the manifold applications of repeat analysis on a genomic scale.
S. Kurtz (2001)
10.1128/JB.182.10.2970-2972.2000
Complete nucleotide sequence of Tn10.
R. Chalmers (2000)
10.1006/PLAS.1993.1020
RepFIB: a basic replicon of large plasmids.
M. Gibbs (1993)
10.1093/nar/gkh121
The Pfam protein families database
A. Bateman (2004)
10.1093/nar/29.1.22
The COG database: new developments in phylogenetic classification of proteins from complete genomes
R. Tatusov (2001)
10.1093/NAR/GKG312
GenDB--an open source genome annotation system for prokaryote genomes.
F. Meyer (2003)
10.1128/MMBR.52.4.433-451.1988
Genetic and physical map of plasmid NR1: comparison with other IncFII antibiotic resistance plasmids.
David D. Womble (1988)
10.1046/j.1365-2958.2000.01975.x
Plasmid and chromosome partitioning: surprises from phylogeny
K. Gerdes (2000)
10.1128/IAI.68.4.2009-2015.2000
Molecular Epidemiologic Approaches to Urinary Tract Infection Gene Discovery in Uropathogenic Escherichia coli
L. Zhang (2000)
Complete nucleotide sequence of Birmingham IncP alpha plasmids. Compilation and comparative analysis.
W. Pansegrau (1994)
D protein of miniF plasmid acts as a repressor of transcription and as a site-specific resolvase.
D. Lane (1986)
10.1007/BF02900361
The staden sequence analysis package
R. Staden (1996)
10.1016/0378-1119(91)90482-q
Identification of eleven single-strand initiation sequences (ssi) for priming of DNA replication in the F, R6K, R100 and ColE2 plasmids.
N. Nomura (1991)
10.1016/0065-227X(86)90018-3
DNA replication of the resistance plasmid R100 and its control.
H. Ohtsubo (1986)
10.1111/j.1365-2958.1992.tb00888.x
A Salmonella dublin virulence plasmid locus that affects bacterial growth under nutrient‐limited conditions
G. Pullinger (1992)
10.1128/JB.175.15.4843-4850.1993
Plasmid pKM101 encodes two nonhomologous antirestriction proteins (ArdA and ArdB) whose expression is controlled by homologous regulatory sequences.
A. A. Belogurov (1993)
10.1128/IAI.68.4.1834-1839.2000
Impact of Siderophore Production onPseudomonas aeruginosa Infections in Immunosuppressed Mice
H. Takase (2000)
10.1126/science.271.5255.1552
A Permease-Oxidase Complex Involved in High-Affinity Iron Uptake in Yeast
R. Stearman (1996)
A permease-oxidase complex involved in highaffinity iron uptake
R. Stearman (1996)
10.1128/JB.171.4.2195-2201.1989
Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.
J. Perez-Casal (1989)
10.1016/S0168-1656(98)00105-9
Horizontal gene transfer as a biosafety issue: a natural phenomenon of public concern.
M. Droege (1998)
10.1016/S0168-6496(03)00164-8
Occurrence of integron-associated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant.
Thomas Tennstedt (2003)
10.1128/JB.183.19.5684-5697.2001
Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas sp. strain ADP.
B. Martínez (2001)
10.1007/s000180050021
Antibiotic resistance in microbes
D. Mazel (1999)
10.1093/NAR/19.6.1203
Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteins.
E. Scherzinger (1991)
10.1111/J.1574-6941.1996.TB00199.X
Spread and evolution of natural plasmids harboring transposon Tn5
J. Blázquez (1996)
10.1006/JMBI.1994.1325
The role of the L-chain in ferritin iron incorporation. Studies of homo and heteropolymers.
S. Levi (1994)
10.1016/S0378-1097(03)00893-0
Identification of novel virulence-associated loci in uropathogenic Escherichia coli by suppression subtractive hybridization.
L. Sorsa (2004)
10.1016/0147-619X(86)90011-9
Distribution of basic replicons having homology with RepFIA, RepFIB, and RepFIC among IncF group plasmids.
P. Bergquist (1986)
- free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate Patc 58
Y. Kawasaki (1996)
10.1007/s00438-002-0785-z
The 79,370-bp conjugative plasmid pB4 consists of an IncP-1β backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene blaNPS-1, and a tripartite antibiotic efflux system of the resistance-nodulation-division family
A. Tauch (2003)
10.1128/JB.119.3.736-747.1974
Culture medium for enterobacteria.
F. C. Neidhardt (1974)
10.1016/0022-2836(85)90228-1
Transcription of the replication control region of the IncFII R-plasmid NR1 in vitro and in vivo.
David D. Womble (1985)
Sequencing and analysis of the large virulence plasmid pLVPK of Klebsiella pneumoniae CG 43
Y. T. Chen (2004)
10.1016/0147-619X(85)90062-9
The development of plasmid-free strains of Agrobacterium tumefaciens by using incompatibility with a Rhizobium meliloti plasmid to eliminate pAtC58.
M. Hynes (1985)
10.1099/MIC.0.27317-0
Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multidrug transport system.
R. Szczepanowski (2004)
The 64508 bp IncP-1 b antibiotic multiresistance plasmid pB 10 isolated from a waste-water treatment plant provides evidence for recombination between members of different branches of the IncP-1 b group
A. Schlüter (2003)
10.1002/PRO.5560030218
Conserved sequence pattern in a wide variety of phosphoesterases
E. Koonin (1994)
10.1128/IAI.55.3.594-599.1987
Virulence of iron transport mutants of Shigella flexneri and utilization of host iron compounds.
K. M. Lawlor (1987)
10.1046/j.1365-2958.1999.01449.x
The selC‐associated SHI‐2 pathogenicity island of Shigella flexneri
J. Moss (1999)



This paper is referenced by
10.1128/AAC.00563-08
Characterization of pUO-StVR2, a Virulence-Resistance Plasmid Evolved from the pSLT Virulence Plasmid of Salmonella enterica Serovar Typhimurium
A. Herrero (2008)
10.1038/icb.2009.31
Salmonella enterica serovar Typhi live vector vaccines finally come of age
J. Galen (2009)
10.1007/978-1-4614-7780-8_4
Mobilized Integrons: Team Players in the Spread of Antibiotic Resistance Genes
E. Martínez (2013)
10.1007/s00216-015-9270-5
Comparative study between macrolide regulatory proteins MphR(A) and MphR(E) in ligand identification and DNA binding based on the rapid in vitro detection system
Yongyou Cheng (2016)
10.1128/JCM.01542-07
Molecular Epidemiology of Escherichia coli Producing Extended-Spectrum β-Lactamases Isolated in Rome, Italy
A. Carattoli (2007)
10.1007/978-1-60327-853-9_5
Persistence mechanisms of conjugative plasmids.
M. Bahl (2009)
Populations of Escherichia coli in clinical samples of Urinary Tract Infections and bacteraemia
F. A. Alhashash (2015)
10.3389/fmicb.2013.00086
Mobile elements, zoonotic pathogens and commensal bacteria: conduits for the delivery of resistance genes into humans, production animals and soil microbiota
S. Djordjevic (2013)
10.1089/mdr.2010.0151
Characterization of plasmids encoding cefotaximases group 1 enzymes in Escherichia coli recovered from cattle in England and Wales.
M. Kirchner (2011)
Chloramphenicol acetyl transferase (cat) defective somatostatin fused protein and using it
Эндрью Р. Менделсон (2008)
10.1007/978-1-4614-7780-8
Lateral Gene Transfer in Evolution
U. Gophna (2013)
10.3201/eid1402.070350
Dissemination of Clonally Related Escherichia coli Strains Expressing Extended-Spectrum β-Lactamase CTX-M-15
T. Coque (2008)
10.1093/jac/dkq181
Molecular characterization of addiction systems of plasmids encoding extended-spectrum beta-lactamases in Escherichia coli.
B. Mnif (2010)
Characterisation of antibiotic resistance gene clusters and their mobility within a collection of multi-drug resistant Salmonella spp
Xiulan Liu (2009)
10.1128/AAC.01540-07
Sequence of Conjugative Plasmid pIP1206 Mediating Resistance to Aminoglycosides by 16S rRNA Methylation and to Hydrophilic Fluoroquinolones by Efflux
B. Périchon (2008)
10.1099/mic.0.028233-0
Detection of 140 clinically relevant antibiotic-resistance genes in the plasmid metagenome of wastewater treatment plant bacteria showing reduced susceptibility to selected antibiotics.
R. Szczepanowski (2009)
10.1073/pnas.0709132105
The evolution of gene collectives: How natural selection drives chemical innovation
M. Fischbach (2008)
10.1007/s00253-014-5511-3
Abundant rifampin resistance genes and significant correlations of antibiotic resistance genes and plasmids in various environments revealed by metagenomic analysis
L. Ma (2014)
10.1016/J.PLASMID.2006.05.005
Complete nucleotide sequences of 84.5- and 3.2-kb plasmids in the multi-antibiotic resistant Salmonella enterica serovar Typhimurium U302 strain G8430.
Chin-Yi Chen (2007)
10.1371/journal.pone.0051160
DNA Sequence Analysis of Plasmids from Multidrug Resistant Salmonella enterica Serotype Heidelberg Isolates
J. Han (2012)
10.1128/JB.00232-07
Multidrug-resistant Salmonella enterica serovar paratyphi A harbors IncHI1 plasmids similar to those found in serovar typhi.
K. Holt (2007)
10.1016/j.plasmid.2009.10.003
Complete sequence of pEC14_114, a highly conserved IncFIB/FIIA plasmid associated with uropathogenic Escherichia coli cystitis strains.
C. DebRoy (2010)
10.1080/10643380902772589
Are Sewage Treatment Plants Promoting Antibiotic Resistance?
K. Jury (2011)
10.1097/MRM.0b013e328346d87d
Acquisition of antimicrobial resistance determinants by virulence plasmids specific for nontyphoid serovars of Salmonella enterica
M. R. Rodicio (2011)
10.1111/J.1574-6976.2007.00074.X
Genomics of IncP-1 antibiotic resistance plasmids isolated from wastewater treatment plants provides evidence for a widely accessible drug resistance gene pool.
A. Schlüter (2007)
the Bacterial World? Successful or Deleterious Association in Antimicrobial Resistance and Virulence: a
Alejandro Beceiro (2013)
10.1016/j.chemosphere.2018.12.130
Genetic variation in the conjugative plasmidome of a hospital effluent multidrug resistant Escherichia coli strain.
C. Ferreira (2019)
10.1128/AAC.00802-06
Novel Macrolide Resistance Module Carried by the IncP-1β Resistance Plasmid pRSB111, Isolated from a Wastewater Treatment Plant
R. Szczepanowski (2006)
10.1128/JCM.00608-10
Population Analysis and Epidemiological Features of Inhibitor-Resistant-TEM-β-Lactamase-Producing Escherichia coli Isolates from both Community and Hospital Settings in Madrid, Spain
O. Martín (2010)
Diversity and epidemiology of plasmids from Enterobacteriaceae from human and non-human reservoirs
E. Bielak (2012)
-Lactamases Isolated in Rome, Italy β Producing Extended-Spectrum Molecular Epidemiology of Escherichia coli
Carlo Mancini (2013)
10.1201/B19549-6
Spread of resistant organisms from human settlements into the environment
Carlos F. Amábile-Cuevas (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar