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Multiplexed Imaging Using Same Species Primary Antibodies With Signal Amplification

Y. Wang, Wenxin Xie, Richie E. Kohman, G. Church
Published 2018 · Chemistry

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Multiplexed IF imaging using same species antibodies is in great demand as multiplexed IF is a general practice in biological and clinical laboratories whilst antibodies from different species are hardly available. We here presented a robust method to eliminate the use of secondary antibodies by replacing secondary antibody signal amplification with DNA-based Hybridization-Chain-Reaction signal amplification. We achieved as much as 89-fold signal enhancement, and four target imaging using all mouse primary antibodies.
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