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Protein-surfactant-polysaccharide Nanoparticles Increase The Catalytic Activity Of An Engineered β-lactamase Maltose-activated Switch Enzyme

J.P. Fuenzalida, T. Xiong, B. M. Moerschbacher, M. Ostermeier, F. Goycoolea
Published 2019 · Biology

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We present polysaccharide-based nanoparticles able to associate and increase the catalytic activity of the maltose-binding MBP317-347 switch enzyme. Fluorescence quenching and molecular docking studies along with the partial resistance to increasing pH and ionic strength indicate that the increase in enzymatic activity is due to a specific interaction between the maltose binding pocket on MBP317-347 and alginate exposed on the surface of the nanoparticles. Finally, we show that the hybrid self co-assembled particles increase the half-life of MBP317-347 over six-fold at 37°C, thus reflecting their potential use as a macromolecular drug delivery system.
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