Online citations, reference lists, and bibliographies.
← Back to Search

Developmental And Thermal Regulation Of The Maize Heat Shock Protein, HSP101.

T. E. Young, J. Ling, C. J. Geisler-Lee, Robert L. Tanguay, C. Caldwell, D. Gallie
Published 2001 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
The plant heat stress protein, Hsp101, and the yeast ortholog, Hsp104, are required to confer thermotolerance in plants and yeast (Saccharomyces cerevisiae), respectively. In addition to its function during stress, Hsp101 is developmentally regulated in plants although its function during development is not known. To determine how the expression of Hsp101 is regulated in cereals, we investigated the Hsp101 expression profile in developing maize (Zea mays). Hsp101 protein was most abundant in the developing tassel, ear, silks, endosperm, and embryo. It was less abundant in the vegetative and floral meristematic regions and was present at only a low level in the anthers and tassel at anthesis, mature pollen, roots, and leaves. As expected, heat treatment resulted in an increase in the level of Hsp101 protein in several organs. In expanding foliar leaves, husk leaves, the tassel at the premeiosis stage of development, or pre-anthesis anthers, however, the heat-mediated increase in protein was not accompanied by an equivalent increase in mRNA. In contrast, the level of Hsp101 transcript increased in the tassel at anthesis following a heat stress without an increase in Hsp101 protein. In other organs such as the vegetative and floral meristematic regions, fully expanded foliar leaves, the young ear, and roots, the heat-induced increase in Hsp101 protein was accompanied by a corresponding increase in Hsp101 transcript level. However, anthers at anthesis, mature pollen, developing endosperm, and embryos largely failed to mount a heat stress response at the level of Hsp101 protein or mRNA, indicating that Hsp101 expression is not heat inducible in these organs. In situ RNA localization analysis revealed that Hsp101 mRNA accumulated in the subaleurone and aleurone of developing kernels and was highest in the root cap meristem and quiescent center of heat-stressed roots. These data suggest an organ-specific control of Hsp101 expression during development and following a heat stress through mechanisms that may include posttranscriptional regulation.
This paper references
The expression of heat shock protein and cognate genes during plant development.
J. Winter (1991)
Polypeptide chain binding proteins: Catalysts of protein folding and related processes in cells
J. Rothman (1989)
Heat shock protein gene expression during embryonic development of the zebrafish
P. Krone (1997)
Heat Shock Proteins and Their mRNAs in Dry and Early Imbibing Embryos of Wheat.
K. W. Helm (1990)
Influence of Temperature Stress on in Vitro Fertilization and Heat Shock Protein Synthesis in Maize (Zea mays L.) Reproductive Tissues.
I. Dupuis (1990)
Hspl04 is a highly conserved protein with two essential nucleotide-binding sites
Dawn A. Parselt (1991)
A soybean 101-kD heat shock protein complements a yeast HSP104 deletion mutant in acquiring thermotolerance.
Y. Lee (1994)
Characterization of two maize HSP90 heat shock protein genes: expression during heat shock, embryogenesis, and pollen development.
K. Marrs (1993)
HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status.
D. R. Wells (1998)
Heat shock protein gene expression during Xenopus development
J. Heikkila (1997)
Characterization of a maize heatshock protein 101 gene , HSP 101 , encoding a ClpB / Hsp 100 protein homologue
J Nieto-Sotelo (1999)
HSP100/Clp proteins: a common mechanism explains diverse functions.
E. C. Schirmer (1996)
An Arabidopsis heat shock protein complements a thermotolerance defect in yeast.
E. C. Schirmer (1994)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding
KS Browning (1976)
Hsp90's secrets unfold: new insights from structural and functional studies.
A. Caplan (1999)
Heat Stress Effects on Isolated Reproductive Organs of Maize
J. C. Mitchell (1988)
The Heat Shock Response in Plants: Short-Term Heat Treatment Regimes and Thermotolerance
R. T. Nagao (1989)
Regulation of heat shock gene induction and expression during Drosophila development
S. Michaud (1997)
Yeast HSP104 homologue rice HSP110 is developmentally- and stress-regulated
S. L. Singla (1997)
Yeast HSP 104 homologue rice HSP 110 is developmentally-and stressregulated
Sl Singla (1997)
Plant Physiol
Characterization of a maize heat-shock protein 101 gene, HSP101, encoding a ClpB/Hsp100 protein homologue.
J. Nieto-Sotelo (1999)
Isolation and Characterization of the 102-Kilodalton RNA-binding Protein That Binds to the 5′ and 3′ Translational Enhancers of Tobacco Mosaic Virus RNA*
Robert L. Tanguay (1996)
Improved method for high efficiency transformation of intact yeast cells.
D. Gietz (1992)
Mutants of Arabidopsis thaliana defective in the acquisition of tolerance to high temperature stress.
S. W. Hong (2000)
Anatomy of Seed Plants
K. Esau (1960)
Tissue specificity of the heat-shock response in maize.
P. Cooper (1984)
Heat Shock Protein HSP101 Binds to the Fed-1 Internal Light Regulatory Element and Mediates Its High Translational Activity
J. Ling (2000)
Isolation and characterization of the 102kilodalton RNA - binding protein that binds to the 5  and 3  translational enhancers of tobacco mosaic virus RNA
RL Tanguay (1996)
Hsp70 expression and function during embryogenesis.
J. Luft (1999)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
Heat‐shock proteins during pollen development in maize
C. Frova (1989)
HSP104 required for induced thermotolerance.
Y. Sánchez (1990)
Translation initiation factors are differentially regulated in cereals during development and following heat shock
D. Gallie (1998)
Heat-shock proteins as molecular chaperones.
J. Becker (1994)
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.
D. A. Parsell (1993)
Hsp104, Hsp70, and Hsp40 A Novel Chaperone System that Rescues Previously Aggregated Proteins
J. R. Glover (1998)
DMSO-enhanced whole cell yeast transformation.
J. Hill (1991)
Synthesis of long, capped transcripts in vitro by SP6 and T7 RNA polymerases.
J. Yisraeli (1989)
Synthesis of Small Heat-Shock Proteins Is Part of the Developmental Program of Late Seed Maturation
N. Wehmeyer (1996)
Protein disaggregation mediated by heat-shock protein Hspl04
D. A. Parsell (1994)
Maize Pollen Viability and Ear Receptivity under Water and High Temperature Stress 1
J. B. Schoper (1986)
Distribu - tion patterns of 104 kDa stressassociated protein in rice
SL Singla (1998)
Identification of two messenger RNA cap binding proteins in wheat germ. Evidence that the 28-kDa subunit of eIF-4B and the 26-kDa subunit of eIF-4F are antigenically distinct polypeptides.
K. Browning (1987)
Heat Shock Protein 101 Plays a Crucial Role in Thermotolerance in Arabidopsis
C. Queitsch (2000)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Hsp 90 ’ s secrets unfold : new insights from structural and functional studies
P Chomczynski (1999)
, HSP 101 , encoding a ClpB / Hsp 100 protein homologue
A Pareek (1995)
Protein folding in the plant cell.
J. Miernyk (1999)
Studies on heat shock proteins in sea urchin development
G. Giudice (1999)
Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores
J. Magnard (1996)
Yeast HSP 104 homologue rice HSP 110 is developmentallyand stressregulated
SL Singla (1997)
In situ hybridization The Maize Handbook
Ja Langdale (1994)
The heatshock response
S Lindquist (1986)
High Temperature Stress and Pollen Viability of Maize 1
M. P. Herrero (1980)
The heat-shock response.
S. Lindquist (1986)
Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone
E. Nielsen (1997)
Cytoplasmic chaperones in precursor targeting to mitochondria: the role of MSF and hsp 70.
K. Mihara (1996)
In situ Hybridization
J. Langdale (1994)

This paper is referenced by
Ovule-specific expression of the genes for mitochondrial and endoplasmic reticulum localized small heat-shock proteins in tomato flower
K. Sanmiya (2005)
Tratamientos robustecedores de semillas para mejorar la emergencia y el crecimiento de Trichospermum mexicanum , Árbol tropical pionero
J. A. Sanchez (2006)
High temperature stress of Brassica napus during flowering reduces micro- and megagametophyte fertility, induces fruit abortion, and disrupts seed production.
L. Young (2004)
Heat Shock Proteins and Acquisition of Thermotolerance in Plants
Mohammad Pessarakli (2010)
High Temperature Stress
M. Djanaguiraman (2014)
Increasing vitamin C content of plants through enhanced ascorbate recycling
Z. Chen (2003)
The 5'-untranslated region of the tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plant.
J. Satoh (2004)
Thioredoxin and germinating barley: targets and protein redox changes
C. Marx (2002)
Section 4 update: In situ hybridization to detect microbial messenger RNA in plant tissues
K. Pawlowski (2004)
An Arabidopsis chloroplast-targeted Hsp101 homologue, APG6, has an essential role in chloroplast development as well as heat-stress response.
F. Myouga (2006)
The 5'-leader of tobacco mosaic virus promotes translation through enhanced recruitment of eIF4F.
D. Gallie (2002)
Responses and Management of Heat Stress in Plants
A. Wahid (2012)
Heat stress in cultivated plants: nature, impact, mechanisms, and mitigation strategies—a review
M. Hassan (2020)
Study of thermotolerance mechanism in Gossypium hirsutum through identification of heat stress genes
J. Zhang (2013)
Heat-shock protein expression in a perennial grass commonly associate with active geothermal areas in western North America
T. S. Al-Niemi (2002)
Effects of Prolonged High Temperature Stress on Respiration , Photosynthesis and Gene Expression in Wheat ( Triticum aestivum L . ) Varieties Differing in their Thermotolerance
M. Almeselmani (2011)
Adapting wheat in Europe for climate change
M. Semenov (2014)
Variation in dynamics of the heat shock proteins HSP70 synthesis in Malva sylvestris and M. pulchella (Malvaceae) in connection with tolerance to high temperature, flooding and drought
L. E. Kozeko (2016)
Lost in Translation : Translation Mechanisms in Production of Cocksfoot Mottle Virus Proteins
K. Mäkeläinen (2006)
The absence of heat shock protein HSP101 affects the proteome of mature and germinating maize embryos.
Pedro E. Lázaro-Mixteco (2012)
Heat-shock protein expression in a perennial grass commonly associated with active geothermal areas in western North America
T. S. Al-Niemi (2002)
Changes in Chlorophyll Fluorescence in Maize Plants with Imposed Rapid Dehydration at Different Leaf Ages
Z. Z. Xu (2007)
The effect of drought and heat stress on reproductive processes in cereals.
B. Barnabás (2008)
Heat Stress in Legume Seed Setting: Effects, Causes, and Future Prospects
Yong-Hua Liu (2019)
Transcriptomic analysis reveals the mechanism of thermosensitive genic male sterility (TGMS) of Brassica napus under the high temperature inducement
X. Tang (2019)
Characterization of 5′UTR of rice ClpB-C/Hsp100 gene: evidence of its involvement in post-transcriptional regulation
R. C. Mishra (2015)
Role of HSP101 in the stimulation of nodal root development from the coleoptilar node by light and temperature in maize (Zea mays L.) seedlings
Guillermo López-Frías (2011)
Genetic regulation of maize and sorghum under abiotic stress
A. L. Renaud (2015)
Heat-tolerant basmati rice engineered by over-expression of hsp101
Surekha Katiyar-Agarwal (2004)
Ectopic expression of Arabidopsis glutaredoxin AtGRXS17 enhances thermotolerance in tomato.
Q. Wu (2012)
Cloning and Expression Analysis of HSP 101 Gene In Bread Wheat (Triticum aestivum L.Em.Thell.)
Manjjri Singal (2014)
Complexity of rice Hsp100 gene family: lessons from rice genome sequence data
Gaurav Batra (2007)
See more
Semantic Scholar Logo Some data provided by SemanticScholar