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Polyamine-induced DNA Synthesis And Mitosis In Oat Leaf Protoplasts.

R. Kaur-Sawhney, H. E. Flores, A. Galston
Published 1980 · Biology, Medicine

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Freshly isolated protoplasts from leaves of oat seedlings (var. Victory) which do not divide when cultured on a wide range of media are capable of incorporating tritiated leucine, uridine, and thymidine into trichloroacetic acid-insoluble macromolecules. Over 70% of the leucine and uridine incorporated over an 18-hour period are found in protein and RNA, respectively, as shown by hydrolysis of the macromolecular products with a specific protease or RNase. In contrast, little or none of the tritiated thymidine is incorporated into macromolecules hydrolyzable by DNase over an 18- to 96-hour period. Incorporation of thymidine into trichloroacetic acid-insoluble material declines sharply with increasing time of culture after 18 hours. However, addition of diamines or polyamines to the medium not only prevents the decline, but actually increases net thymidine incorporation, including a fraction going into DNA. A significant increase in mitoses and binucleate protoplasts is also observed in 72- to 168-hour cultures.The inability of oat leaf protoplasts to synthesize significant quantities of DNA suggests that they are arrested at the G(1) phase of the cell cycle. Treatment with polyamines appears to promote both DNA synthesis and the inception of mitotic activity in oat protoplasts, as in numerous animal and microbial cells.
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10.1088/1755-1315/388/1/012067
Response of Gerbera (Gerbera jamesonii) cv. ‘Great Smoky Mountains’ to Foliar Application of Putrescine, Spermidine and Salicylic Acid
A. Saeed (2019)
10.3389/fpls.2019.01033
Nexus Between Spermidine and Floral Organ Identity and Fruit/Seed Set in Tomato
Savithri U. Nambeesan (2019)
10.29278/azd.476202
Derim öncesi putresin uygulamasının 0900 Ziraat kiraz çeşidinin meyve kalitesi üzerine etkileri
D. Erbaş (2018)
10.1007/978-1-4939-7398-9_40
Potential Applications of Polyamines in Agriculture and Plant Biotechnology.
A. Tiburcio (2018)
MOLECULAR ANALYSIS OF JASMONIC ACID & POLYAMINES SYNTHESIS & SIGNALING UNDER HEAT & DROUGHT STRESS IN RICE
D. K. Umesh (2017)
10.7324/japs.2015.50106
Antioxidant Properties, Secondary metabolites and Growth as affected by application of Putrescine and Moringa leaves extract on Jojoba plants
L. S. Taha (2015)
10.3389/fpls.2015.00413
Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’
B. Chiancone (2015)
Research article ROLE OF POLYAMINES IN RETARDATION OF DARK INCUBATION INDUCED ALTERATIONS IN THE PRIMARY PROCESSES OF PHOTOSYNTHESIS IN MAIZE (ZEA MAYS) LEAVES.
G. Bhanumathi (2014)
10.1016/j.jphotobiol.2011.02.007
Polyamines interaction with thylakoid proteins during stress.
S. Hamdani (2011)
10.1007/s11738-011-0842-5
Modulation of Arabidopsis CYCB1 expression patterns by polyamines and salt stress
M. A. Ortega-Amaro (2011)
Stimulation effect of some bioregulators on flowering , chemical constituents , essential oil and phytohormones of tuberose
S. Taha Lobna (2011)
Stimulation effect of some bioregulators on flowering, chemical constituents, essential oil and phytohormones of tuberose (Polianthes tuberos L.).
A. Eid (2011)
Effect of some Simulative Compounds on Growth, Yield and Chemical Composition of Snap Bean Plants Grown under Calcareous Soil Conditions
H. Ahmed (2010)
THE EFFECTS OF PRETREATMENTS OF SOME PLANT GROWTH REGULATORS ON GERMINATION AND SEEDLING GROWTH OF RADISH SEEDS UNDER SALINE CONDITIONS
B. Bölümü (2009)
10.1556/ABiol.59.2008.1.8
Effects of polyamines and polyamine biosynthetic inhibitors on mitotic activity of Allium cepa root tips.
M. Unal (2008)
10.1007/BF02925785
Induction of adventitious buds on the cotyledons ofAbies concolor x Abies grandis hybrid seedlings
B. Vooková (2008)
10.1007/BF02632192
Cereal protoplast recalcitrance
A. Cutler (2007)
10.1007/BF02632131
Comparison of cell wall regeneration on maize protoplasts isolated from leaf tissue and suspension cultured cells
Hong Wang (2007)
EFFECTS OF SOME PLANT GROWTH REGULATORS ON JASMONIC ACID INDUCED INHIBITION OF SEED GERMINATION AND SEEDLING GROWTH OF BARLEY
K. Kabar (2007)
10.1079/IVP2005654
The story of transgenic cereals: The challenge, the debate, and the solution—A historical perspective
I. Vasil (2007)
THE EFFECTS OF PRETREATMENTS OF SOME PLANT GROWTH REGULATORS ON GERMINATION AND SEEDLING GROWTH OF RADISH SEEDS UNDER SALINE CONDITIONS
K. Çavuşoğlu (2007)
10.1016/J.SCIENTA.2007.05.006
Effect of exogenous putrescine on post-harvest life of strawberry (Fragaria ananassa Duch.) fruit, cultivar Selva
M. R. Khosroshahi (2007)
EFFECTS OF SOME PLANT GROWTH REGULATORS ON JASMONIC ACID INDUCED INHIBITION OF SEED GERMINATION AND SEEDLING GROWTH OF BARLEY
K. Çavuşoğlu (2007)
Transgenic Plants as Tool to Study the Evolution of Pyrrolizidine Alkaloids
A. Elhady (2006)
10.1104/pp.106.083030
Consistency of Polyamine Profiles and Expression of Arginine Decarboxylase in Mitosis during Zygotic Embryogenesis of Scots Pine1
J. Vuosku (2006)
10.1007/BF02318966
Influence of exogenous proline on embryogenic and organogenic maize callus subjected to salt stress
M. Santos (2006)
10.1639/0007-2745(2005)108[110:TIOSOT]2.0.CO;2
The Influence of Spermine on the Activity of a Protein Kinase C-like Enzyme in Marchantia polymorpha Thalli During Programmed Cell Death
Justin J. Seaman (2005)
10.1007/BF01352920
Microtubules in maize leaf protoplasts in relation to donor tissue and in vitro culture
H. Wang (2005)
10.1007/BF00195077
Effect of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves
R. Besford (2004)
10.1007/BF00117595
The occurrence and possible function of hydroxycinnamoyl acid amides in plants
J. Martin-Tanguy (2004)
10.1007/BF00033463
DNA replication in maize leaf protoplasts
H. Wang (2004)
10.1007/BF00117590
Polyamine levels as related to growth, differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia and Avena sativa
R. Sawhney (2004)
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