Online citations, reference lists, and bibliographies.
← Back to Search

Reassociation Kinetics And Cytophotometric Characterization Of Peanut (Arachis Hypogaea L.) DNA.

S. S. Dhillon, A. Rake, J. P. Miksche
Published 1980 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The base composition of peanut (var. NC-17) DNA determined from thermal denaturation profiles showed an average guanine plus cystosine content of 34% which was in close approximation to 36% guanine plus cytosine calculated from the buoyant density. Buoyant density also indicated the absence of satellite DNA. The genome size, 2.0 x 10(9) base pairs, as determined by reassociation kinetics of the single copy DNA was close to the genome size determined by cytophotometry, 2.1 x 10(9) base pairs. Peanut DNA averaging 450 to 600 base pairs long, reassociated in phosphate buffer and fractionated by hydroxylapatite, indicated a DNA genome composition of 36% nonrepetitive or single copy DNA; reassociation in formamide and followed by optical methods indicated the repetitive DNA possesses highly repeated, intermediately repeated and rarely repeated components of DNA with DNA sequences repeated on the average about 38,000, 6,700, and 200 times each. Different criteria of reassociation in formamide revealed further subdivisions of these four separate components of DNA. The DNA of above mentioned NC-17 variety compared to Florigiant variety showed no differences in thermal denaturation profiles, buoyant density, or in genome size.
This paper references
10.1016/0005-2787(67)90056-1
The satellite DNA's of some higher plants.
B. Green (1967)
10.1104/PP.62.1.112
Kinetic determination of the genome size of the pea.
W. Pearson (1978)
10.1139/M68-040
Use of normal probability paper in determining thermal melting values of deoxyribonucleic acid.
M. D. Knittel (1968)
Genome organization in higher plants
RB FLAVELL (1975)
10.1021/BI00619A027
DNA sequence organization in the pea genome.
M. G. Murray (1978)
10.1021/ed059p1079
Equilibrium density gradient centrifugation for introductory biochemistry
Carl Wm. Vermeulen (1982)
10.1104/PP.55.3.496
The Relationship between Satellite Deoxyribonucleic Acid, Ribosomal Ribonucleic Acid Gene Redundancy, and Genome Size in Plants.
J. Ingle (1975)
10.1002/J.1537-2197.1977.TB07613.X
REQUIREMENT OF AN INTERNAL STANDARD FOR MICROSPECTROPHOTOMETRIC MEASUREMENTS OF DNA
Sukhraj S. Dhillon (1977)
10.1016/B978-0-12-147601-4.50011-7
3 – Ultrastructural Organization of Plant Cell Nuclei
J. Lafontaine (1974)
10.1016/0022-2836(76)90242-4
Developmental biochemistry of cotton seed embryogenesis and germination. VII. Characterization of the cotton genome.
V. Walbot (1976)
Deoxyribonucleic and base composition of some angiosperms and its taxonomic significance
GP BERLYN (1970)
10.1126/SCIENCE.451548
Regulation of gene expression: possible role of repetitive sequences.
E. Davidson (1979)
10.2307/3573493
The nature of residual Escherichia coli DNA after degradation induced by ionizing radiation.
A. Rake (1972)
10.1016/0005-2787(74)90157-9
Physical studies on synthetic DANs containing 5-methylcytosine
J. E. Gill (1974)
10.1007/978-3-662-12523-6
Modern Methods in Forest Genetics
J. P. Miksche (1976)
10.1104/PP.57.4.617
Aggregate formation from short fragments of plant DNA.
W. F. Thompson (1976)
10.1016/0005-2787(79)90500-8
Sequence organization of the soybean genome.
W. Gurley (1979)
10.2135/CROPSCI1973.0011183X001300060036X
Characterization of Chloroplast and Nuclear DNAs of Zea mays L. 1
D. Shah (1973)
Quantitative determination of DNA in cells by Feulgen microspectrophotometry.
C. Leuchtenberger (1958)
10.1021/BI00640A020
Characterization of families of repeated DNA sequences from four vascular plants.
A. Bendich (1977)
Evolution of repetitive and nonrepetitive DNA
GA GALAU (1976)
10.1007/978-3-662-12523-6_1
Optical Techniques for Measuring DNA Quantity
G. Berlyn (1976)
10.1016/S0031-9422(00)85757-X
Deoxyribonucleic acid base composition of some angiosperms and its taxonomic significance
S. Biswas (1970)
The nucleus and the organization and transcription of nuclear DNA
D GRIERSON (1977)
10.1126/SCIENCE.165.3891.349
Gene regulation for higher cells: a theory.
R. Britten (1969)
10.1021/BI00836A024
Nucleic acid reassociation in formamide.
B. McConaughy (1969)
10.1016/S0022-2836(67)80087-1
Effect of methylation of cytosine residues on the buoyant density of DNA in caesium chloride solution.
J. Kirk (1967)
10.1038/257152A0
Localisation of satellite DNA sequences in nuclei and chromosomes of two plants
J. N. Timmis (1975)
10.1139/G80-010
DNA REASSOCIATION KINETICS OF FOUR CONIFERS
A. Rake (1980)
10.1073/PNAS.73.2.415
Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.
R. Britten (1976)
10.1126/SCIENCE.161.3841.529
Repeated Sequences in DNA
R. Britten (1968)
10.1073/PNAS.72.12.4805
Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.
M. Smith (1975)
10.1016/0076-6879(67)12133-2
[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA
M. Mandel (1968)
10.1073/PNAS.49.6.897
A relationship between DNA content, nuclear volume, and minimum mitotic cycle time.
J. Van't Hof (1963)
10.1007/978-3-662-12523-6_2
Nucleic Acid Extraction, Purification, Reannealing, and Hybridization Methods
R. Hall (1976)
10.1016/0076-6879(74)29033-5
Analysis of repeating DNA sequences by reassociation.
R. Britten (1974)
10.1177/27.10.92496
Nonspecific light loss and intrinsic DNA variation problems associated with feulgen DNA cytophotometry.
J. P. Miksche (1979)



This paper is referenced by
10.1038/ng.3517
The genome sequences of Arachis duranensis and Arachis ipaensis, the diploid ancestors of cultivated peanut
D. Bertioli (2016)
10.1002/J.1537-2197.1985.TB08309.X
DNA-RNA, and protein comparisons between nodulated and non-nodulated male-sterile and male-fertile genotypes of soybean (Glycine max L.)
Deogratis E. Artis (1985)
10.1007/978-1-4613-1661-9_17
DNA Analysis During Growth and Development
S. S. Dhillon (1988)
10.1007/BF00716849
Stable ploidy levels in long-term callus cultures of loblolly pine
C. I. Franklin (2004)
10.1007/978-3-319-63935-2_10
Functional Genomics in Peanut Wild Relatives
P. Guimarães (2017)
10.1093/aob/mct128
The repetitive component of the A genome of peanut (Arachis hypogaea) and its role in remodelling intergenic sequence space since its evolutionary divergence from the B genome.
D. Bertioli (2013)
10.1111/pbi.13175
Whole‐genome resequencing‐based QTL‐seq identified AhTc1 gene encoding a R2R3‐MYB transcription factor controlling peanut purple testa colour
Y. Zhao (2019)
10.3389/fpls.2019.00883
Major QTLs for Resistance to Early and Late Leaf Spot Diseases Are Identified on Chromosomes 3 and 5 in Peanut (Arachis hypogaea)
Y. Chu (2019)
10.1038/s41588-019-0405-z
The genome sequence of segmental allotetraploid peanut Arachis hypogaea
D. Bertioli (2019)
10.3146/AT07-006.1
Genomics: An Evolving Science in Peanut
H. T. Stalker (2009)
10.3389/fpls.2018.00604
Genome Sequencing and Analysis of the Peanut B-Genome Progenitor (Arachis ipaensis)
Q. Lu (2018)
10.1201/9781439822265.ch6
Genomics and Genetic Enhancement of Peanut
A. Paterson (2004)
10.1007/978-3-319-63935-2_9
Sequencing Ancestor Diploid Genomes for Enhanced Genome Understanding and Peanut Improvement
S. Nayak (2017)
10.1128/AEM.56.3.782-787.1990
High diversity in DNA of soil bacteria.
V. Torsvik (1990)
10.1007/s10722-014-0193-3
Genome sizes in diploid and allopolyploid Arachis L. species (section Arachis)
S. S. Samoluk (2014)
10.1007/978-3-319-63935-2_4
Cytological Features of Peanut Genome
G. Seijo (2017)
10.1007/BF01276594
The reserve proteins in the cells of mature cotyledons ofLupinus albus var.Lucky
M. L. Gal (2005)
10.1016/0305-1978(89)90036-7
Comparative analysis of repetitive DNA in five Arachis species
C. D. Atreya (1989)
A Method of Extraction of D N A from Birch
D. Howland (2007)
Genetic Analysis of Resistance to Rosette Disease of Groundnut (Arachis hypogaea L.)
U. Alhassan (2013)
10.1086/337828
Meristematic Activity and Leaf Initiation in the Shoot Apex of Nicotiana tabacum during Floral Transition
J. Thomas (1990)
10.5772/23891
Development of DNA Based Active Macro–Materials for Biology and Medicine: A Review
F. X. Jiang (2011)
10.1007/978-1-0716-0235-5_1
Updates on Legume Genome Sequencing.
J. Ha (2020)
Genome sizes in diploid and allopolyploid Arachis L. species (section Arachis)
S. Sebastian (2014)
Patent Number : 45 Date of Patent : 5 , 545 , 546 Aug . 13 , 1996 54 POLLEN-SPECIFIC PROMOTER FROM MAIZE
R. L. Allen (2017)
10.1111/J.1399-3054.1981.TB04480.X
DNA changes during sequential leaf senescence of tobacco (Nicotiana tabacum)
S. S. Dhillon (1981)
10.31742/IJGPB.79.1.8
Molecular characterization of groundnut (Arachis hypogaea L.) germplasm lines and varietal set for yield and yield attributing traits
Anushree Pramanik (2019)
10.1002/J.1537-2197.1983.TB07932.X
The variability of nuclear DNA and its implications for polyploidy in white ash (Fraxinus americana L.: Oleaceae)
Candace L. Black (1983)
10.3390/genes12010002
Genetic Diversity and Genome-Wide Association Study of Seed Aspect Ratio Using a High-Density SNP Array in Peanut (Arachis hypogaea L.)
Kunyan Zou (2020)
1 Genomics : An Evolving Science in Peanut
H. T. Stalker (2009)
10.3897/CompCytogen.v12i1.20334
The genome structure of Arachis hypogaea (Linnaeus, 1753) and an induced Arachis allotetraploid revealed by molecular cytogenetics
Eliza F. de M. B. do Nascimento (2018)
10.1007/978-3-319-63935-2
The Peanut Genome
R. Varshney (2017)
See more
Semantic Scholar Logo Some data provided by SemanticScholar