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Identification Of Catabolites Of Chlorophyll-Porphyrin In Senescent Rape Cotyledons

S. Ginsburg, P. Matile
Published 1993 · Biology, Medicine

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Developing shoots of rape seedlings (Brassica napus L.) were excised and fed with 4-[14C]5-aminolevulinic acid to label the pyrroles in chlorophyll (Chl) synthesized during the final phase of expansion and greening of the cotyledons. About 80% of 14C taken up into the cotyledons was incorporated into Chl. The subsequent incubation of labeled shoots in permanent darkness caused the rapid loss of labeled Chl while increasing proportions of 14C appeared in the fraction of water-soluble compounds. Reversed-phase high performance liquid chromatography resolved three nonfluorescent polar catabolites of Chl-porphyrin that were progressively accumulated as senescence advanced. At intermediate stages of senescence, the cotyledons contained a fluorescent radio-active derivative of Chl that was also detectable, together with traces of other putative fluorescent catabolites, in isolated senescent chloroplasts. The nonfluorescent catabolites, identified by means of radiolabeling, were also found to accumulate in attached cotyledons senescing under photoperiod; under these conditions, one of the compounds, NCC-1, was particularly abundant. The catabolites of rape exhibited the same ultraviolet spectra, characterized by a maximum at 320 nm, as a previously reported secoporphinoid catabolite from barley (B. Krautler, B. Jaun, W. Amrein, K. Bortlik, M. Schellenberg, P. Matile [1992] Plant Physiol Biochem 30: 333–346). Different polarities suggest, however, that the structures may be different. A terminology for Chl catabolites is proposed because present knowledge suggests that a large number of different structures results from species-specific processing of breakdown products and may require a suitable nomenclature.
This paper references
Proteolytic enzymes and leaf senescence
M J Dalling (1987)
10.1021/JA00201A050
Structure of dinoflagellate luciferin and its enzymic and nonenzymic air-oxidation products
H. Nakamura (1989)
Chlorophyllabbau in vergilbenden Gerstenblattem: Entwicklung und Anwendung einer Methode zur 14C-Markierung von Chlorophyll
C Peisker (1989)
Plant Senescence: Its Biochemistry and Physiology
William W. Thomson (1987)
10.1016/S0176-1617(87)80081-0
Catabolites of Chlorophyll in Senescent Leaves
P. Matile (1987)
10.1042/BST0110591
Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents
H. Lichtenthaler (1983)
10.1016/S0176-1617(11)80214-2
Breakdown of Chlorophyll in Chloroplasts of Senescent Barley Leaves Depends on ATP
M. Schellenberg (1990)
On chlorophyll break - down in senescent leaves
Y Park (1973)
10.1016/S0176-1617(11)81659-7
A Novel Type of Chlorophyll Catabolite in Senescent Barley Leaves
K. Bortlik (1990)
10.1016/S0176-1617(89)80099-9
Phytol and the Breakdown of Chlorophyll in Senescent Leaves
Christian Peisker (1989)
Metabolism of thylakoid membrane proteins during foliar senescence
H Thomas (1987)
10.1073/PNAS.85.24.9529
Catabolites of chlorophyll in senescing barley leaves are localized in the vacuoles of mesophyll cells.
P. Matile (1988)
10.1016/0003-2697(87)90529-X
Decoloration and solubilization of plant tissue prior to determination of 3H, 14C, and 35S by liquid scintillation.
I. K. Smith (1987)
10.1002/ANIE.199113151
On the enigma of chlorophyll degradation:the constitution of a secoporphinoid catabolite.
B. Kräutler (1991)
10.1016/S0176-1617(11)80211-7
Radiolabelling of Chlorophyll for Studies on Catabolism
Christian Peisker (1990)
10.1021/JA00216A070
Structure of the light emitter in krill (Euphausia pacifica) bioluminescence
H. Nakamura (1988)
10.1146/ANNUREV.PP.34.060183.001325
Chlorophyll Biosynthesis: Recent Advances and Areas of Current Interest
and P A Castelfranco (1983)
10.1016/S0176-1617(88)80043-9
Vacuolar Location of Lipofuscin- and Proline-Like Compounds in Senescent Barley Leaves
Theres Düggelin (1988)
Breakdown of chlorophyll : constitution of a secoporphinoid chlorophyll catabolite isolated from senescent barley leaves
B. Kräutler (1992)
Structure of dinoflagellate luciferin and its enzymatic air - oxidation products
H PlantaNakamura (1989)
How and why is chlorophyll broken down in senescent leaves ?
P Matile (1989)
10.1016/0014-5793(91)81168-8
Chlorophyll catabolism in Chlorella protothecoides Isolation and structure elucidation of a red bilin derivative
N. Engel (1991)



This paper is referenced by
10.1088/1757-899X/180/1/012137
Solution Concentration and Flow Rate of Fe3+-modified Porphyrin (Red Blood Model) on Giant Magnetoresistance (GMR) Sensor Efficiency
Ahmad Aminudin (2017)
10.1007/s10535-007-0088-7
Leaf senescence and activities of the antioxidant enzymes
D. Procházková (2007)
10.1080/07352689409701916
Nitrogen Metabolism in Senescing Leaves
U. Feller (1994)
10.1016/S1360-1385(00)01735-0
Degradation pathway(s) of chlorophyll: what has gene cloning revealed?
K. Takamiya (2000)
10.1111/J.1469-8137.1994.TB02953.X
Stay‐green genotypes of Phaseolus vulgaris L.: chloroplast proteins and chlorophyll catabolites during foliar senescence
A. Bachmann (1994)
Chlorophyll degradation in wheat lines elicited by cereal aphid infestation
T. Wang (2003)
10.1016/J.POSTHARVBIO.2005.07.002
Mechanism of soybean (Glycine max L. Merrill) degreening related to maturity stage and postharvest drying temperature
P. Sinnecker (2005)
10.1016/S0031-9422(98)00001-6
NCC malonyltransferase catalyses the final step of chlorophyll breakdown in rape (Brassica napus).
S. Hörtensteiner (1998)
10.1021/jf4054947
Quantitation of chlorophylls and 22 of their colored degradation products in culinary aromatic herbs by HPLC-DAD-MS and correlation with color changes during the dehydration process.
Jean-Louis Lafeuille (2014)
10.1111/j.1469-8137.2008.02412.x
Nondestructive analysis of senescence in mesophyll cells by spectral resolution of protein synthesis-dependent pigment metabolism.
A. Gay (2008)
10.1562/0031-8655(2000)0710084IAICOH2.0.CO2
Isolation and Initial Characterization of 132‐Hydroxychlorophyll a Induced by Cyclohexanedione Derivatives in Tobacco Cell Suspension Cultures
Jing‐Ming Wang (2000)
10.1109/ISPTS.2012.6260887
Chlorophyll based biosensor for sulfur mustard - a chemical warfare agent
Simerjit Kaur (2012)
10.1105/tpc.106.044404
In Vivo Participation of Red Chlorophyll Catabolite Reductase in Chlorophyll Breakdown[W]
Adriana Pružinská (2007)
10.1104/pp.115.2.677
Partial Purification and Characterization of Red Chlorophyll Catabolite Reductase, a Stroma Protein Involved in Chlorophyll Breakdown
S. Rodoni (1997)
10.1034/J.1399-3054.1999.100105.X
Chlorophyll catabolism and gene expression in the peel of ripening banana fruits
R. Drury (1999)
10.1111/tpj.13253
A liquid chromatography-mass spectrometry platform for the analysis of phyllobilins, the major degradation products of chlorophyll in Arabidopsis thaliana.
Bastien Christ (2016)
10.1007/978-94-007-5724-0_16
The Pathway of Chlorophyll Degradation: Catabolites, Enzymes and Pathway Regulation
S. Hörtensteiner (2013)
10.1104/pp.105.2.545
Cleavage of Chlorophyll-Porphyrin (Requirement for Reduced Ferredoxin and Oxygen)
S. Ginsburg (1994)
10.11606/T.9.2009.TDE-25092009-150741
FACULDADE DE CIÊNCIAS FARMACÊUTICAS Programa de Pós-Graduação em Ciência dos Alimentos Área de Bromatologia Efeito do déficit hídrico em características químicas e bioquímicas da soja e na degradação da clorofila, com ênfase na formação de metabólitos incolores
Daniela Borrmann (2009)
10.1603/0022-0493-97.2.661
Enzymatic Chlorophyll Degradation in Wheat Near-Isogenic Lines Elicited by Cereal Aphid (Homoptera: Aphididae) Feeding
T. Wang (2004)
10.1104/pp.115.2.669
Chlorophyll Breakdown in Senescent Chloroplasts (Cleavage of Pheophorbide a in Two Enzymic Steps)
S. Rodoni (1997)
10.1111/J.1469-8137.1995.TB04294.X
Chlorophyll breakdown in senescent leaves: identification of the biochemical lesion in a stay-green genotype of Festuca pratensis Huds.
F. Vicentini (1995)
10.1074/jbc.271.44.27233
How Plants Dispose of Chlorophyll Catabolites
B. Hinder (1996)
10.1155/2015/490627
Systematic HPLC/ESI-High Resolution-qTOF-MS Methodology for Metabolomic Studies in Nonfluorescent Chlorophyll Catabolites Pathway
J. Ríos (2015)
10.1007/s004250050199
Isolation of cDNA clones for genes showing enhanced expression in barley leaves during dark-induced senescence as well as during senescence under field conditions
T. Kleber‐Janke (1997)
10.1007/s000180050434
Chlorophyll breakdown in higher plants and algae
S. Hörtensteiner (1999)
10.1016/S0031-9422(00)90394-7
Carotenoid composition and its relationship to chlorophyll and D1 protein during leaf development in a normally senescing cultivar and a stay-green mutant of Festuca pratensis
B. Biswal (1994)
10.1007/BF00195702
Production of a presumptive chlorophyll catabolite in vitro: requirement for reduced ferredoxin
M. Schellenberg (2004)
10.1016/0031-9422(95)00012-V
Evidence for the involvement of superoxide anion in the ethylene-induced chlorophyll a catabolism of Raphanus sativus cotyledons
M. Adachi (1995)
10.1093/JXB/49.320.503
Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L.
Z. Fang (1998)
10.1016/J.FOODRES.2014.03.063
Non-fluorescent chlorophyll catabolites in quince fruits
J. Ríos (2014)
10.2503/JJSHS.68.1139
The Ethylene-Induced Chlorophyll Catabolism of Radish (Raphanus sativus L.) Cotyledons : Production of Colorless Fluorescent Chlorophyll Catabolite (FCC) In vitro
M. Adachi (1999)
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