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Vascularization, High-Volume Solution Flow, And Localized Roles For Enzymes Of Sucrose Metabolism During Tumorigenesis By Agrobacterium Tumefaciens1

R. Wächter, M. Langhans, R. Aloni, S. Götz, Anke Weilmünster, A. Koops, Leopoldine Temguia, I. Mistrík, J. Pavlovkin, U. Rascher, Katja Schwalm, K. Koch, C. Ullrich
Published 2003 · Biology, Medicine

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Vascular differentiation and epidermal disruption are associated with establishment of tumors induced by Agrobacterium tumefaciens. Here, we address the relationship of these processes to the redirection of nutrient-bearing water flow and carbohydrate delivery for tumor growth within the castor bean (Ricinus communis) host. Treatment with aminoethoxyvinyl-glycine showed that vascular differentiation and epidermal disruption were central to ethylene-dependent tumor establishment. CO2 release paralleled tumor growth, but water flow increased dramatically during the first 3 weeks. However, tumor water loss contributed little to water flow to host shoots. Tumor water loss was followed by accumulation of the osmoprotectants, sucrose (Suc) and proline, in the tumor periphery, shifting hexose-to-Suc balance in favor of sugar signals for maturation and desiccation tolerance. Concurrent activities and sites of action for enzymes of Suc metabolism changed: Vacuolar invertase predominated during initial import of Suc into the symplastic continuum, corresponding to hexose concentrations in expanding tumors. Later, Suc synthase (SuSy) and cell wall invertase rose in the tumor periphery to modulate both Suc accumulation and descending turgor for import by metabolization. Sites of abscisic acid immunolocalization correlated with both central vacuolar invertase and peripheral cell wall invertase. Vascular roles were indicated by SuSy immunolocalization in xylem parenchyma for inorganic nutrient uptake and in phloem, where resolution allowed SuSy identification in sieve elements and companion cells, which has widespread implications for SuSy function in transport. Together, data indicate key roles for ethylene-dependent vascularization and cuticular disruption in the redirection of water flow and carbohydrate transport for successful tumor establishment.
This paper references
The three - dimensional structure of vascular tissues in Agrobacterium tumefaciensinduced crown galls and in the host stems of Ricinus communis L
KS Pradel (1995)
Sucrose protects cell wall invertase but not vacuolar invertase against proteinaceous inhibitors
A. Sander (1996)
Sucrose Modulation of Soybean Vsp Gene Expression Is Inhibited by Auxin
D. Dewald (1994)
The phloem, a miracle of ingenuity
A. V. Bel (2003)
Ethylene production and ACC‐accumulation in Agrobacterium tumefaciens‐induced plant tumours and their impact on tumour and host stem structure and function
R. Wächter (1999)
Cytosolic cycles regulate the turnover of sucrose in heterotrophic cell-suspension cultures of Chenopodium rubrum L.
J. Dancer (2004)
Regulation of source/sink relations by cytokinins
T. Roitsch (2004)
The Dual Function of Sugar Carriers: Transport and Sugar Sensing
S. Lalonde (1999)
Growth of the Maize Primary Root at Low Water Potentials : II. Role of Growth and Deposition of Hexose and Potassium in Osmotic Adjustment.
R. Sharp (1990)
Ivr 2 , a candidate gene for a QTL of vacuolar invertase activity in maize leaves : genespecific expression under water stress
S Pelleschi (1999)
An enzyme‐immunoassay for cis‐(+)‐abscisic acid
E. Weiler (1982)
Sugar-induced signal transduction
S Smeekens (2000)
Symplastic continuity in Agrobacterium tumefaciens-induced tumours
K. Pradel (1999)
Interactions between Abscisic Acid and Ethylene Signaling Cascades
N. Beaudoin (2000)
Induction of crown galls by Agrobacterium tumefaciens (strain C58) reverses assimilate translocation and accumulation in Kalanchoë daigremontiana
S. Malsy (1992)
Eine störungsfreie Mikromethode zur Bestimmung des Proteingehaltes in Gewebehomogenaten
N Popov (1975)
Companion-Cell Specific Localization of Sucrose Synthase in Zones of Phloem Loading and Unloading
K. D. Nolte (1993)
Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition.
H. Hansen (2000)
Impaired sucroseinduction mutants reveal the modulation of sugarinduced starch biosynthetic gene expression by abscisic acid signaling
F Rook (2001)
Sugars as Signal Molecules in Plant Seed Development
U. Wobus (1999)
Co-ordinated induction of mRNAs for extracellular invertase and a glucose transporter in Chenopodium rubrum by cytokinins.
R. Ehness (1997)
Development - and organspecific expression of the genes for sucrose synthase and three isoenzymes of acid fructosidase in carrot
A Sturm (1995)
Physiological Site of Ethylene Effects on Carbon Dioxide Assimilation in Glycine max L. Merr.
G. E. Taylor (1988)
Changes in fructan content in developing barley caryopses
G Büttner (1985)
Temperature profiles for the expression of endogenous rhythmicity and arrhythmicity of CO2 exchange in the CAM plant Kalanchoë daigremontiana can be shifted by slow temperature changes
U. Rascher (1998)
Maize sucrose synthase1 promoter directs phloemcell specific expression of Gus gene in transgenic tobacco plants
NS Yang (1990)
Immunolocalization of plasma-membrane H+-ATPase and tonoplast-type pyrophosphatase in the plasma membrane of the sieve element-companion cell complex in the stem of Ricinus communis L.
M. Langhans (2001)
Immunolocalization of plasmamembrane H ATPase and tonoplasttype pyrophosphatase in the plasma membrane of the sieve elementcompanion cell complex in the stem of Ricinus communis L
M Langhans (2001)
Sucrose is metabolised by sucrose synthase and glycolysis within the phloem complex of Ricinus communis L. seedlings
P. Geigenberger (2004)
Flavonoid-related regulation of auxin accumulation in Agrobacterium tumefaciens-induced plant tumors
Katja Schwalm (2003)
A novel sucrose synthase pathway for sucrose degradation in cultured sycamore cells.
S. Huber (1986)
Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
R. Aloni (2002)
Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes.
K. Koch (1992)
Immunolocalization of plasmamembrane H ϩ -ATPase and tonoplast-type pyrophosphatase in the plasma membrane of the sieve element-companion cell complex in the stem of Ricinus communis L
M Langhans (2001)
Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants
P. Zambryski (1989)
Cell wall invertase in tobacco crown gall cells : enzyme properties and regulation by auxin.
M. Weil (1990)
The never ripe mutant provides evidence that tumor-induced ethylene controls the morphogenesis of agrobacterium tumefaciens-induced crown galls on tomato stems
Aloni (1998)
Biochemical Adaptation: Mechanism and Process in Physiological Evolution
P. Hochachka (1984)
Identification and localization of transformed cells in Agrobacterium tumefaciens-induced plant tumors
Claudia Rezmer (1999)
Eine störungsfreie Mikromethode zur Bestimmung des Proteingehaltes in Gewebehomoge - naten
N Popov (1975)
Stomatal conductance and photosynthe
GD Farquhar (1982)
Identification and localization of transformed cells in Agrobacterium tumefaciensinduced plant tumors
C Rezmer (1999)
Evidence for Symplastic Phloem Unloading with Concomitant High Activity of Acid Cell Wall Invertase in Agrobacterium tumefaciens‐Induced Plant Tumors*
K. Pradel (1996)
Sucrose metabolism during Agrobacterium tumefaciens — induced tumor growth in sunflower hypocotyls
U. Kutschera (2000)
Development of Agrobacterium tumefaciens C58-induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid, auxin, cytokinin, ethylene and abscisic acid
D. Veselov (2002)
Ivr2, a candidate gene for a QTL of vacuolar invertase activity in maize leaves. Gene-specific expression under water stress
S. Pelleschi (2004)
A Similar Dichotomy of Sugar Modulation and Developmental Expression Affects Both Paths of Sucrose Metabolism: Evidence from a Maize Invertase Gene Family.
J. Xu (1996)
Sugar and hormone connections.
P. León (2003)
A maize vacuolar invertase, IVR2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression.
J. Y. Kim (2000)
Inhibition of photosynthesis by ethylene-a stomatal effect.
J. E. Pallas (1982)
Develop - ment of Agrobacterium tumefaciens C 58 - induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid , auxin , cytokinin , ethylene , and abscisic acid
D Veselov (2003)
Ivr 2 , a candidate gene for a QTL of vacuolar invertase activity in maize leaves : gene - specific expression under water stress
S Pelleschi (1999)
The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning.
Sturm (1999)
A photometric method for the determination of proline.
W. Troll (1955)
EcFLO, a FLORICAULA-like gene from Eschscholzia californica is expressed during organogenesis at the vegetative shoot apex
Andrea Busch (2003)
S. Smeekens (2000)
K. Koch (1996)
Impaired sucrose-induction mutants reveal the modulation of sugar-induced starch biosynthetic gene expression by abscisic acid signalling.
F. Rook (2001)
The three-dimensional structure of vascular tissues in Agrobacterium tumefaciens-induced crown galls and in the host stems of Ricinus communis L.
R. Aloni (2004)
Structural and Functional Evidence for Xylem‐Mediated Water Transport and High Transpiration in Agrobacterium tumefaciens‐Induced Tumors of Ricinus communis*
U. Schurr (1996)
Develop - ment of Agrobacterium tumefaciens C 58induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid , auxin , cytokinin , ethylene , and abscisic acid
D Veselov (2003)
Development- and organ-specific expression of the genes for sucrose synthase and three isoenzymes of acid β-fructofuranosidase in carrot
A. Sturm (2004)
The inception phase in the crown gall disease.
R. Beardsley (1972)
Maize sucrose synthase-1 promoter directs phloem cell-specific expression of Gus gene in transgenic tobacco plants.
N. Yang (1990)
Vascularization is a general requirement for growth of plant and animal tumours.
C. Ullrich (2000)
Regulation of Abscisic Acid Signaling by the Ethylene Response Pathway in Arabidopsis
M. Ghassemian (2000)
The Plant Invertases: Physiology, Biochemistry and Molecular Biology
Z. Tymowska-Lalanne (1998)
Stomatal conductance and photosynthesis
G. Farquhar (1982)
Hormone genes and crown gall disease
E. Weiler (1987)
Impact of Agrobacterium tumefaciens-induced stem tumors on NO3− uptake in Ricinus communis
I. Mistrík (2004)
Evidence for high activity of xylem parenchyma and ray cells in the interface of host stem and Agrobacterium tumefaciens-induced tumours of Ricinus communis.
J. Pavlovkin (2002)
Interrelationships of ethylene and abscisic Acid in the control of rose petal senescence.
S. Mayak (1972)

This paper is referenced by
Structural basis of a novel repressor, SghR, controlling Agrobacterium infection by cross-talking to plants
F. Ye (2020)
Molecular characterization and expression of four cDNAs encoding sucrose synthase from green bamboo Bambusa oldhamii.
Wen-Bin Chiu (2006)
Examining the roles of sucrose synthase isoforms in Arabidopsis growth and development
Danyu Yao (2019)
Non-structural Carbohydrate Metabolism in the Flesh of Stone Fruits of the Genus Prunus (Rosaceae) – A Review
R. P. Walker (2020)
The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug12
T. Tzfira (2003)
A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases
L. Torres (2007)
Plant responses to Agrobacterium tumefaciens and crown gall development
J. Gohlke (2014)
Complexities of invertases controlling sucrose accumulation and retention in sugarcane
A. Chandra (2012)
Gall Phenotypes – Product of Plant Cells Defensive Responses to the Inducers Attack
R. Isaias (2012)
An Integrated View of Gene Expression and Solute Profiles of Arabidopsis Tumors: A Genome-Wide Approach[W]
R. Deeken (2006)
Walls around tumours — why plants do not develop cancer
J. Doonan (2010)
Reduction of polar auxin transport in tobacco by the tumorigenic Agrobacterium tumefaciens AK-6b gene
Yasutaka Kakiuchi (2005)
Detection and growth of endophytic entomopathogenic fungi in dicot crop plants
C. Ullrich (2017)
Sucrose and invertases, a part of the plant defense response to the biotic stresses
Alexandra S. Tauzin (2014)
Cytokinin deficiency causes distinct changes of sink and source parameters in tobacco shoots and roots
T. Werner (2008)
Biology of Crown Gall Tumors
R. Aloni (2008)
Determination of Structural Requirements and Probable Regulatory Effectors for Membrane Association of Maize Sucrose Synthase 11
S. C. Hardin (2006)
de-açúcar: sinalização de açúcares
Escola Superior de Agricultura (2012)
Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens
E. Haudecoeur (2009)
Agrobacterium tumefaciens Promotes Tumor Induction by Modulating Pathogen Defense in Arabidopsis thaliana[W]
Chil-Woo Lee (2009)
LeFRK2 is required for phloem and xylem differentiation and the transport of both sugar and water
H. Damari-Weissler (2009)
Arabidopsis sucrose synthase localization indicates a primary role in sucrose translocation in phloem
Danyu Yao (2019)
Ecological Conditions and Molecular Determinants Involved in Agrobacterium Lifestyle in Tumors
T. Meyer (2019)
The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites.
Almudena González-Mula (2019)
Cytological and histochemical gradients induced by a sucking insect in galls of Aspidosperma australe Arg. Muell (Apocynaceae)
D. C. Oliveira (2010)
Agrobacterium tumefaciens responses to plant-derived signaling molecules
Sujatha Subramoni (2014)
Plant Defence: Biological Control
J. Mérillon (2012)
Sweet connections, bZIP transcription factors and the regulation of metabolism in Arabidopsis
M.G.M Hanssen (2009)
Genetic transformation of Torenia fournieri L. mediated by Agrobacterium rhizogenes
Jun Tao (2006)
Functional Gradients in Insect Gall Tissues: Studies on Neotropical Host Plants
D. C. Oliveira (2014)
Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream.
R. Aloni (2005)
Lateral ABA transport in maize roots (Zea mays): visualization by immunolocalization.
Daniela Schraut (2004)
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