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Hydrogen Peroxide Generation By The Pepper Extracellular Peroxidase CaPO2 Activates Local And Systemic Cell Death And Defense Response To Bacterial Pathogens1[W][OA]
Hyong Woo Choi, Y. Kim, S. Lee, J. Hong, B. Hwang
Published 2007 · Biology, Medicine
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Reactive oxygen species (ROS) are responsible for mediating cellular defense responses in plants. Controversy has existed over the origin of ROS in plant defense. We have isolated a novel extracellular peroxidase gene, CaPO2, from pepper (Capsicum annuum). Local or systemic expression of CaPO2 is induced in pepper by avirulent Xanthomonas campestris pv vesicatoria (Xcv) infection. We examined the function of the CaPO2 gene in plant defense using the virus-induced gene silencing technique and gain-of-function transgenic plants. CaPO2-silenced pepper plants were highly susceptible to Xcv infection. Virus-induced gene silencing of the CaPO2 gene also compromised hydrogen peroxide (H2O2) accumulation and hypersensitive cell death in leaves, both locally and systemically, during avirulent Xcv infection. In contrast, overexpression of CaPO2 in Arabidopsis (Arabidopsis thaliana) conferred enhanced disease resistance accompanied by cell death, H2O2 accumulation, and PR gene induction. In CaPO2-overexpression Arabidopsis leaves infected by Pseudomonas syringae pv tomato, H2O2 generation was sensitive to potassium cyanide (a peroxidase inhibitor) but insensitive to diphenylene iodonium (an NADPH oxidase inhibitor), suggesting that H2O2 generation depends on peroxidase in Arabidopsis. Together, these results indicate that the CaPO2 peroxidase is involved in ROS generation, both locally and systemically, to activate cell death and PR gene induction during the defense response to pathogen invasion.
This paper references
Early signaling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca 2 1
LV Bindschedler (2001)
Vascular defense responses in rice: peroxidase accumulation in xylem parenchyma cells and xylem wall thickening.
E. Hilaire (2001)
Virus-induced gene silencing in tomato.
Yule Liu (2002)
A method of high frequency virus-induced gene silencing in chili pepper (Capsicum annuum L. cv. Bukang).
E. Chung (2004)
Subcellular localization of H 2 O 2 in plants : H 2 O 2 accumulation in papillae and hypersensitive response during the barleypowdery mildew interaction
H Thordal-Christensen (1997)
Function of the oxidative burst in hypersensitive disease resistance.
R. Tenhaken (1995)
parative biochemistry of the oxidative burst produced by rose and French bean cells reveals two distinct mechanisms
GP Bolwell (1998)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
Early signaling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca 21
L V Bindschedler (2001)
Fast forward genetics based on virus-induced gene silencing.
D. Baulcombe (1999)
Recent advances in understanding the origin of the apoplastic oxidative burst in plant cells.
G. Bolwell (1999)
Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: A novel, rapid defense response
D. Bradley (1992)
Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction
Tomonori Kawano (2003)
Nicotiana benthamiana gp91phox Homologs NbrbohA and NbrbohB Participate in H2O2 Accumulation and Resistance to Phytophthora infestans Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.008680.
H. Yoshioka (2003)
Convenient Assay of O 2 - Generated on Potato Tuber Tissue Slices Treated with Fungal Elicitor by Electron Spin Resonance - No Secondary Oxidative Burst Induction by H 2 O 2 Treatment
Hae-jun Park (2005)
Properties, Functions, and Secretion of Human Myeloperoxidase
J. Arnhold (2004)
The respiratory burst of phagocytes.
B. Babior (1984)
Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death.
I. Murgia (2004)
rbohA, a rice homologue of the mammalian gp91phox respiratory burst oxidase gene.
Q. Groom (1996)
Hydroperoxide assay with the ferricxylenol orange complex
C Gay (1999)
Reactive oxygen signaling: the latest news
C Laloi (2004)
Nicotiana benthamiana gp91 phox homologs NbrbohA and NbrbohB participate in H 2 O 2 accumulation and resistance to Phytophthora infestans
H Yoshioka (2003)
Peroxidase-dependent apoplastic oxidative burst in Arabidopsis required for pathogen resistance.
L. Bindschedler (2006)
Short communication: subcellular localization of ozone-induced hydrogen peroxide production in birch (Betula pendula) leaf cells.
R. Pellinen (1999)
Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components
N. Doke (1983)
RbohA, a rice homologue of mammalian pg91 phox respiratory burst oxidase gene
O J Groom (1996)
Arabidopsis RIN 4 is a target of the type III virulence effector AvrRpt 2 and modulates RPS 2mediated resistance
D Mackey (2003)
Comparative biochemistry of the oxidative burst produced by rose and french bean cells reveals two distinct mechanisms
Active Oxygen Species in Plant Defense against Pathogens
M. Mehdy (1994)
Molecular Mechanisms Involved in Bacterial Speck Disease Resistance of Tomato
Y. Kim (2004)
Reactive oxygen intermediates as mediators of programmed cell death in plants and animals.
T. Jabs (1999)
Cadmium-induced subcellular accumulation of O2·− and H2O2 in pea leaves
M. C. Romero-Puertas (2004)
Short communication. Harpin and hydrogen peroxide induce the expression of a homologue of gp91-phox in Arabidopsis thaliana suspension cultures
R. Deskian (1998)
Lesion mimic mutants: keys for deciphering cell death and defense pathways in plants?
Séverine Lorrain (2003)
Subcellular localization of ozoneinduced hydrogen peroxide production in birch (Betula pendula) leaf cells
R Pellinen (1999)
Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction
H. Thordal-Christensen (1997)
A plant homologue of the neutrophil NADPH-oxidase gp91 phox subunit gene encodes a plasma membrane protein with Ca 21 binding motifs
T Keller (1998)
Hydroperoxide assay with the ferric-xylenol orange complex.
C. Gay (1999)
A large family of class III plant peroxidases.
Susumu Hiraga (2001)
Harpin and hydrogen peroxide induce the expression of a homologue of gp91-phox in Arabidopsis thaliana suspension cultures
R. Desikan (1998)
Hydrogen Peroxide Acts as a Second Messenger for the Induction of Defense Genes in Tomato Plants in Response to Wounding, Systemin, and Methyl Jasmonate
M. Orozco-Cárdenas (2001)
The plant immune system
J. Jones (2006)
Expression of peroxidase-like genes, H2O2 production, and peroxidase activity during the hypersensitive response to Xanthomonas campestris pv. vesicatoria in Capsicum annuum.
Hyun Mee Do (2003)
Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colletotrichum lagenarium
R. Hammerschmidt (1982)
Synthesis of dehydrogenation polymers of ferulic acid with high specificity by a purified cell-wall peroxidase from French bean (Phaseolus vulgaris L.).
A. Zimmerlin (1994)
Resistance gene-dependent plant defense responses.
K. Hammond-Kosack (1996)
Nicotiana benthamiana gp 91 phox homologs NbrbohA and NbrbohB participate in H 2 O 2 accumulation and resistance to Phytophthora infestans
H Yoshioka (2003)
Induction of some defense-related genes and oxidative burst is required for the establishment of systemic acquired resistance in Capsicum annuum
S. Lee (2005)
Reactive Oxygen Intermediates Mediate a Systemic Signal Network in the Establishment of Plant Immunity
M. E. Alvarez (1998)
Performing the paradoxical: how plant peroxidases modify the cell wall.
F. Passardi (2004)
The Genetic Basis of Singlet OxygenInduced Stress Responses of Arabidopsis thaliana
D. Wagner (2004)
Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response
M. A. Torres (2001)
Isolation, partial sequencing, and expression of pathogenesis-related cDNA genes from pepper leaves infected by Xanthomonas campestris pv. vesicatoria.
H. W. Jung (2000)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
Mechanisms for the generation of reactive oxygen species in plant defence – a broad perspective
G. Bolwell (1997)
RbohA , a rice homologue of mammalian pg 91 phox respiratory burst oxidase gene
OJ Groom (1996)
Reactive oxygen species: metabolism, oxidative stress, and signal transduction.
K. Apel (2004)
The hypersensitive response: a cell death during disease resistance
Jeong-Mee Park (2005)
Arabidopsis RIN4 Is a Target of the Type III Virulence Effector AvrRpt2 and Modulates RPS2-Mediated Resistance
David Mackey (2003)
Functions of the respiratory burst oxidase in biotic interactions, abiotic stress and development.
M. A. Torres (2005)
Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2
L. Bindschedler (2001)
Cauliflower mosaic virus, a Compatible Pathogen of Arabidopsis, Engages Three Distinct Defense-Signaling Pathways and Activates Rapid Systemic Generation of Reactive Oxygen Species1
A. J. Love (2005)
Peroxidases have more functions than a Swiss army knife
F. Passardi (2005)
A Plant Homolog of the Neutrophil NADPH Oxidase gp91phox Subunit Gene Encodes a Plasma Membrane Protein with Ca2+ Binding Motifs
T. Keller (1998)
The RPM1 plant disease resistance gene facilitates a rapid and sustained increase in cytosolic calcium that is necessary for the oxidative burst and hypersensitive cell death.
M. Grant (2000)
Hydrogen peroxide is involved in abscisic acid-induced stomatal closure in Vicia faba.
X. Zhang (2001)
Ozone: Changing Anthracnose (caused by Colletotrichum acutatum) Severity and Accelerating Hypersensitive Response in Pepper
Yun Sung-Chul (2006)
A plant homologue of the neutrophil NADPHoxidase gp 91 phox subunit gene encodes a plasma membrane protein with Ca 2 1 binding motifs
T Keller (1998)
Pathogen-induced, NADPH oxidase–derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana
M. A. Torres (2005)
THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE.
C. Lamb (1997)
Reactive oxygen signalling: the latest news.
C. Laloi (2004)
A H2O2-forming peroxidase rather than a NAD(P)H-dependent O2•- synthase may be the major player in cell death responses controlled by the Pto-Fen complex following fenthion treatment.
M. D. Biasi (2003)
Pepper gene encoding a basic pathogenesis‐related 1 protein is pathogen and ethylene inducible
Y. Kim (2000)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
Arabidopsis is susceptible to infection by a downy mildew fungus.
E. Koch (1990)
Recent advances in understanding the origin of the cell wall oxidative burst in plant cells
G P Bolwell (1999)
Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox).
M. A. Torres (1998)
This paper is referenced by
The Pepper Mannose-Binding Lectin Gene CaMBL1 Is Required to Regulate Cell Death and Defense Responses to Microbial Pathogens1[C][W][OA]
I. S. Hwang (2011)
Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide.
Ilya Gadjev (2008)
COMPARISON OF INDUCED GENE RESPONSE TO STRESSFUL TREATMENT IN SPINACIA OLERACEA AND BRASSICA NAPUS
S. Navabpour (2011)
Structural-functional features of plant isoperoxidases
I. Maksimov (2011)
A Salutary Role of Reactive Oxygen Species in Intercellular Tunnel-Mediated Communication
Dacheng Liang (2018)
Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst
Ádám Czobor (2019)
The pepper calmodulin gene CaCaM1 is involved in reactive oxygen species and nitric oxide generation required for cell death and the defense response.
Hyong Woo Choi (2009)
XopN-T3SS effector of Xanthomonas axonopodis pv. punicae localizes to the plasma membrane and modulates ROS accumulation events during blight pathogenesis in pomegranate.
R. Kumar (2016)
Requirement of the Cytosolic Interaction between PATHOGENESIS-RELATED PROTEIN10 and LEUCINE-RICH REPEAT PROTEIN1 for Cell Death and Defense Signaling in Pepper[W]
Du Seok Choi (2012)
Systemic Signalling in Plant Defence
Emily E Helliwell (2009)
Epigenetic Changes are Associated with Programmed Cell Death Induced by Heat Stress in Seedling Leaves of Zea mays.
P. Wang (2015)
Genes/Quantitative Trait Loci and Associated Molecular Mechanisms Identified in Capsicum Genome for Tolerance to Abiotic and Biotic Stresses
Vandana Jaiswal (2019)
The Class III Peroxidase (POD) Gene Family in Cassava: Identification, Phylogeny, Duplication, and Expression
Chunlai Wu (2019)
Damaged-self recognition in common bean (Phaseolus vulgaris) shows taxonomic specificity and triggers signaling via reactive oxygen species (ROS)
Dalia Duran-Flores (2014)
Correlative evidence for the involvement of phenolic compounds and peroxidase in resistance against Phytophthoracolocasiaeleaf blight in Colocasiaesculenta(taro)
Jim J. Mo (2015)
Plant Defense and Long‐Distance Signaling in the Phloem
Frank Gaupels (2012)
Apoplastic immunity and its suppression by filamentous plant pathogens.
G. Doehlemann (2013)
Dual Roles of Reactive Oxygen Species and NADPH Oxidase RBOHD in an Arabidopsis-Alternaria Pathosystem1[W]
M. Pogány (2009)
GLYCINE-RICH RNA-BINDING PROTEIN1 interacts with RECEPTOR-LIKE CYTOPLASMIC PROTEIN KINASE1 and suppresses cell death and defense responses in pepper (Capsicum annuum).
D. Kim (2015)
Phosphite-induced suppression of Pseudomonas bleeding canker (Pseudomonas syringae pv. aesculi) of horse chestnut (Aesculus hippocastanum L.)
G Criselda Percival (2015)
CaCDPK15 positively regulates pepper responses to Ralstonia solanacearum inoculation and forms a positive-feedback loop with CaWRKY40 to amplify defense signaling
Lei Shen (2016)
Running head: ROS, NO and calcium cross-talk under Cd toxicity
Luisa M Sandalio (2009)
Hydrogen Peroxide- and Nitric Oxide-mediated Disease Control of Bacterial Wilt in Tomato Plants
J. Hong (2013)
The pepper patatin-like phospholipase CaPLP1 functions in plant cell death and defense signaling
D. Kim (2013)
A comparison of colorimetric assays detecting hydrogen peroxide in leaf extracts
A. Mátai (2017)
Xanthomonas filamentous hemagglutinin-like protein Fha1 interacts with pepper hypersensitive-induced reaction protein CaHIR1 and functions as a virulence factor in host plants.
Hyong Woo Choi (2013)
Overview of Biotic Stresses in Pepper (Capsicum spp.): Sources of Genetic Resistance, Molecular Breeding and Genomics
M. Parisi (2020)
Role of class III peroxidase PRX34 in plant oxidative burst and immunity
L. Zhao (2019)
Reactive Oxygen Species in Plant–Pathogen Interactions
G. Bolwell (2009)
Activity of defense related enzymes and gene expression in pigeon pea (Cajanus cajan) due to feeding of Helicoverpa armigera larvae
A. L. Meitei (2018)
Differential expression of duplicated peroxidase genes in the allotetraploid Brassica napus.
J. Zhao (2009)
Signaling and Communication in Plants
S. Mugnai (2011)See more