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Chlorophyll Degradation: The Tocopherol Biosynthesis-Related Phytol Hydrolase In Arabidopsis Seeds Is Still Missing1[C][W][OPEN]

W. Zhang, T. Liu, G. Ren, S. Hörtensteiner, Y. Zhou, E. Cahoon, C. Zhang
Published 2014 · Biology, Medicine

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Two known chlorophyll-related types of phytol hydrolases may only play limited roles in tocopherol biosynthesis in Arabidopsis seeds, implicating another unknown dephytylase that may exist. Phytyl diphosphate (PDP) is the prenyl precursor for tocopherol biosynthesis. Based on recent genetic evidence, PDP is supplied to the tocopherol biosynthetic pathway primarily by chlorophyll degradation and sequential phytol phosphorylation. Three enzymes of Arabidopsis (Arabidopsis thaliana) are known to be capable of removing the phytol chain from chlorophyll in vitro: chlorophyllase1 (CLH1), CLH2, and pheophytin pheophorbide hydrolase (PPH), which specifically hydrolyzes pheophytin. While PPH, but not chlorophyllases, is required for in vivo chlorophyll breakdown during Arabidopsis leaf senescence, little is known about the involvement of these phytol-releasing enzymes in tocopherol biosynthesis. To explore the origin of PDP for tocopherol synthesis, seed tocopherol concentrations were determined in Arabidopsis lines engineered for seed-specific overexpression of PPH and in single and multiple mutants in the three genes encoding known dephytylating enzymes. Except for modestly increasing tocopherol content observed in the PPH overexpressor, none of the remaining lines exhibited significantly reduced tocopherol concentrations, suggesting that the known chlorophyll-derived phytol-releasing enzymes do not play major roles in tocopherol biosynthesis. Tocopherol content of seeds from double mutants in NONYELLOWING1 (NYE1) and NYE2, regulators of chlorophyll degradation, had modest reduction compared with wild-type seeds, although mature seeds of the double mutant retained significantly higher chlorophyll levels. These findings suggest that NYEs may play limited roles in regulating an unknown tocopherol biosynthesis-related phytol hydrolase. Meanwhile, seeds of wild-type over-expressing NYE1 had lower tocopherol levels, suggesting that phytol derived from NYE1-dependent chlorophyll degradation probably doesn’t enter tocopherol biosynthesis. Potential routes of chlorophyll degradation are discussed in relation to tocopherol biosynthesis.
This paper references
10.1126/SCIENCE.1132912
Cross-Species Identification of Mendel's I Locus
I. Armstead (2007)
10.1105/tpc.105.037077
The Arabidopsis vitamin E pathway gene5-1 Mutant Reveals a Critical Role for Phytol Kinase in Seed Tocopherol Biosynthesis[W][OA]
H. Valentin (2005)
10.1016/j.febslet.2007.10.060
The chlorophyllases AtCLH1 and AtCLH2 are not essential for senescence‐related chlorophyll breakdown in Arabidopsis thaliana
N. Schenk (2007)
10.1104/PP.89.4.1028
Accumulation of α-Tocopherol in Senescing Organs as Related to Chlorophyll Degradation
M. Rise (1989)
10.3390/metabo3020347
Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos
Eva Collakova (2013)
10.1016/j.tplants.2009.01.002
Stay-green regulates chlorophyll and chlorophyll-binding protein degradation during senescence.
S. Hörtensteiner (2009)
10.1046/J.1365-313X.1998.00343.X
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
10.1104/PP.010421
Isolation and functional analysis of homogentisate phytyltransferase from Synechocystis sp. PCC 6803 and Arabidopsis.
E. Collakova (2001)
10.1046/J.1365-313X.1999.00637.X
Chlorophyll breakdown by chlorophyllase: isolation and functional expression of the Chlase1 gene from ethylene-treated Citrus fruit and its regulation during development.
D. Jacob-Wilk (1999)
Metabolic compartmentation of plastid prenyllipid biosynthesis--evidence for the involvement of a multifunctional geranylgeranyl reductase.
Y. Keller (1998)
10.1104/pp.108.124933
Citrus Chlorophyllase Dynamics at Ethylene-Induced Fruit Color-Break: A Study of Chlorophyllase Expression, Posttranslational Processing Kinetics, and in Situ Intracellular Localization1[OA]
Tamar Azoulay Shemer (2008)
10.1105/tpc.112.095588
Fatty Acid Phytyl Ester Synthesis in Chloroplasts of Arabidopsis[W]
F. Lippold (2012)
10.1016/S1360-1385(00)01735-0
Degradation pathway(s) of chlorophyll: what has gene cloning revealed?
K. Takamiya (2000)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1007/s11103-008-9314-8
Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway
Sylvain Aubry (2008)
10.1111/j.1365-313X.2010.04417.x
Vitamin E biosynthesis: functional characterization of the monocot homogentisate geranylgeranyl transferase.
W. Yang (2011)
10.1093/mp/ssu045
Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation during leaf senescence.
Y. Sakuraba (2014)
10.1111/tpj.12067
Genetic and biochemical basis for alternative routes of tocotrienol biosynthesis for enhanced vitamin E antioxidant production.
C. Zhang (2013)
10.1105/tpc.107.050633
Chlorophyllase Is a Rate-Limiting Enzyme in Chlorophyll Catabolism and Is Posttranslationally Regulated
Smadar Harpaz-Saad (2007)
10.1104/PP.119.4.1507
Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco.
I. García (1999)
10.1105/tpc.108.064089
Pheophytin Pheophorbide Hydrolase (Pheophytinase) Is Involved in Chlorophyll Breakdown during Leaf Senescence in Arabidopsis[W][OA]
S. Schelbert (2009)
Citrus chloro - phyllase dynamics at ethylene - induced fruit color - break : a study of chlorophyllase expression , posttranslational processing kinetics , and in situ intracellular localization
T AzoulayShemer (2008)
10.1146/annurev.arplant.50.1.67
CHLOROPHYLL DEGRADATION.
P. Matile (1999)
10.1105/tpc.111.089474
STAY-GREEN and Chlorophyll Catabolic Enzymes Interact at Light-Harvesting Complex II for Chlorophyll Detoxification during Leaf Senescence in Arabidopsis[C][W]
Y. Sakuraba (2012)
10.1074/jbc.M509222200
A Salvage Pathway for Phytol Metabolism in Arabidopsis*
Till Ischebeck (2006)
10.1046/J.1365-313X.2001.01153.X
Use of red fluorescent protein from Discosoma sp. (dsRED) as a reporter for plant gene expression.
G. Jach (2001)
Identi fi - cation of a novel chloroplast protein AtNYE 1 regulating chlorophyll degradation during leaf senescence in Arabidopsis
M Rise (1989)
10.1016/J.TPLANTS.2004.06.002
From Arabidopsis to agriculture: engineering improved Vitamin E content in soybean.
S. Sattler (2004)
10.1146/ANNUREV.ARPLANT.57.032905.105212
Chlorophyll degradation during senescence.
S. Hörtensteiner (2006)
10.1073/PNAS.96.26.15362
Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate.
T. Tsuchiya (1999)
10.1016/0003-9861(80)90066-1
Tocopherol and plastoquinone synthesis in spinach chloroplasts subfractions.
J. Soll (1980)
10.1016/S0304-3940(02)01423-4
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data
C. Ramakers (2003)
10.1046/J.1432-1327.1998.2510413.X
Metabolic compartmentation of plastid prenyllipid biosynthesis
Y. Keller (1998)
10.1104/pp.107.100172
Identification of a Novel Chloroplast Protein AtNYE1 Regulating Chlorophyll Degradation during Leaf Senescence in Arabidopsis1[C][W][OA]
G. Ren (2007)
10.1126/SCIENCE.1086391
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
J. Alonso (2003)
10.1016/0924-2244(96)81352-5
Chlorophyll degradation in processed foods and senescent plant tissues
James W. Heaton (1996)
10.1002/(SICI)1521-4133(199910)101:10<400::AID-LIPI400>3.0.CO;2-#
Tocopherols accumulation in developing seeds and pods of rapeseed (Brassica napus L.)
F. Goffman (1999)
10.1104/pp.106.085647
Quantitative Profiling of Arabidopsis Polar Glycerolipids in Response to Phosphorus Starvation. Roles of Phospholipases Dζ1 and Dζ2 in Phosphatidylcholine Hydrolysis and Digalactosyldiacylglycerol Accumulation in Phosphorus-Starved Plants1[W]
M. Li (2006)
10.1073/pnas.1308114110
ABI3 controls embryo degreening through Mendel's I locus
F. Delmas (2013)
10.1042/BST0300625
Unravelling chlorophyll catabolism in higher plants.
B. Kräutler (2002)
10.1073/pnas.0705521104
Mendel's green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway
Y. Sato (2007)



This paper is referenced by
10.1016/j.molp.2015.12.016
NON-YELLOWING2 (NYE2), a Close Paralog of NYE1, Plays a Positive Role in Chlorophyll Degradation in Arabidopsis.
Shouxin Wu (2016)
10.3389/fpls.2015.00871
Temporal analysis reveals a key role for VTE5 in vitamin E biosynthesis in olive fruit during on-tree development
E. C. Georgiadou (2015)
10.1101/257519
A role for phylloquinone biosynthesis in the plasma membrane as revealed in a non-photosynthetic parasitic plant
X. Gu (2018)
10.1021/acs.est.7b02163
Metabolomics Reveals Cu(OH)2 Nanopesticide-Activated Anti-oxidative Pathways and Decreased Beneficial Antioxidants in Spinach Leaves.
L. Zhao (2017)
10.1016/j.jplph.2020.153195
Chlorophyll Metabolism and Gene Expression in Response to Submergence Stress and Subsequent Recovery in Perennial Ryegrass Accessions Differing in Growth Habits.
L. Gan (2020)
10.3389/fpls.2017.01959
Metabolic Origins and Transport of Vitamin E Biosynthetic Precursors
Sébastien Pellaud (2017)
Identification of a Chlorophyll Salvage Pathway and a Novel Chlorophyllase in Arabidopsis
林耀斌 (2015)
10.1038/s41598-020-59840-4
Screening of microalgae liquid extracts for their bio stimulant properties on plant growth, nutrient uptake and metabolite profile of Solanum lycopersicum L.
Chanda Mutale-joan (2020)
10.1016/j.bbabio.2015.02.002
The role of plastoglobules in thylakoid lipid remodeling during plant development.
Sarah Rottet (2015)
10.1021/ACS.EST.6B04647
Metabolomics Reveals Cryptic Interactive Effects of Species Interactions and Environmental Stress on Nitrogen and Sulfur Metabolism in Seagrass.
H. Hasler-Sheetal (2016)
10.1016/BS.ABR.2019.03.004
Chlorophyll breakdown—Regulation, biochemistry and phyllobilins as its products
S. Hörtensteiner (2019)
10.1007/s00216-017-0596-z
Metabolic changes in primary, secondary, and lipid metabolism in tobacco leaf in response to topping
Jieyu Zhao (2017)
10.1021/acs.jafc.5b02787
A Novel Recombinant Chlorophyllase1 from Chlamydomonas reinhardtii for the Production of Chlorophyllide Derivatives.
Yi-Li Chou (2015)
10.1007/s00425-019-03160-z
Transcriptome analysis of shade avoidance and shade tolerance in conifers
S. Ranade (2019)
10.1016/J.ETI.2015.09.002
Evaluation of chlorophyll and anti-oxidative components harvested from the anaerobic digestion of fruit and vegetable waste
Hsiao-Dao Chang (2015)
10.1016/j.copbio.2017.01.007
Current strategies for vitamin E biofortification of crops.
Laurent Méné-Saffrané (2017)
10.1021/acs.jafc.7b01306
Comparative Metabolic Response between Cucumber ( Cucumis sativus) and Corn ( Zea mays) to a Cu(OH)2 Nanopesticide.
L. Zhao (2018)
The role of alpha-tocopherol in the protection of tomato plants against abiotic stress
Livia Spicher (2017)
10.1104/pp.114.239541
Different Mechanisms Are Responsible for Chlorophyll Dephytylation during Fruit Ripening and Leaf Senescence in Tomato1[W][OPEN]
L. Guyer (2014)
10.1534/g3.119.400838
Interaction Between Induced and Natural Variation at oil yellow1 Delays Reproductive Maturity in Maize
Rajdeep S. Khangura (2019)
10.1080/15592324.2017.1382797
Supraoptimal activity of CHLOROPHYLL DEPHYTYLASE1 results in an increase in tocopherol level in mature arabidopsis seeds
Y. Lin (2017)
10.1101/706846
Interaction between induced and natural variation at oil yellow1 delays flowering in maize
Rajdeep S. Khangura (2019)
10.1111/pbi.12889
Beyond pathways: genetic dissection of tocopherol content in maize kernels by combining linkage and association analyses
H. Wang (2018)
10.1111/pbi.13362
Beyond the limits of photoperception: constitutively active PHYTOCHROME B2 overexpression as a means of improving fruit nutritional quality in tomato
F. R. Alves (2020)
10.1104/pp.18.00727
Time-Course Transcriptome Analysis of Arabidopsis Siliques Discloses Genes Essential for Fruit Development and Maturation1
Chiara Mizzotti (2018)
10.1016/j.chemosphere.2019.125616
Metabolic profiles in the course of the shikimic acid pathway of Raphanus sativus var. longipinnatus exposed to mesotrione and its degradation products.
J. Płonka (2019)
10.14348/molcells.2015.0039
The Divergent Roles of STAYGREEN (SGR) Homologs in Chlorophyll Degradation
Y. Sakuraba (2015)
10.1016/j.phytochem.2014.11.007
Fruits from ripening impaired, chlorophyll degraded and jasmonate insensitive tomato mutants have altered tocopherol content and composition.
J. Almeida (2015)
10.1105/tpc.17.00475
Novel Loci Underlie Natural Variation in Vitamin E Levels in Maize Grain[OPEN]
Christine H. Diepenbrock (2017)
10.1105/tpc.16.00478
Identification of a Chlorophyll Dephytylase Involved in Chlorophyll Turnover in Arabidopsis[OPEN]
Y. Lin (2016)
10.1093/pcp/pcw021
Pheophytinase Knockdown Impacts Carbon Metabolism and Nutraceutical Content Under Normal Growth Conditions in Tomato.
Bruno S Lira (2016)
10.3389/fpls.2016.01656
Regulation of On-Tree Vitamin E Biosynthesis in Olive Fruit during Successive Growing Years: The Impact of Fruit Development and Environmental Cues
E. C. Georgiadou (2016)
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