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G Protein Activation Stimulates Phospholipase D Signaling In Plants.

T. Munnik, S. A. Arisz, T. de Vrije, A. Musgrave
Published 1995 · Biology, Medicine

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We provide direct evidence for phospholipase D (PLD) signaling in plants by showing that this enzyme is stimulated by the G protein activators mastoparan, ethanol, and cholera toxin. An in vivo assay for PLD activity in plant cells was developed based on the use of a "reporter alcohol" rather than water as a transphosphatidylation substrate. The product was a phosphatidyl alcohol, which, in contrast to the normal product phosphatidic acid, is a specific measure of PLD activity. When 32P-labeled cells were treated with 0.1% n-butanol, 32P-phosphatidyl butanol (32P-PtdBut) was formed in a time-dependent manner. In cells treated with any of the three G protein activators, the production of 32P-PtdBut was increased in a dose-dependent manner. The G protein involved was pertussis toxin insensitive. Ethanol could activate PLD but was itself consumed by PLD as transphosphatidylation substrate. In contrast, secondary alcohols (e.g., sec-butyl alcohol) activated PLD but did not function as substrate, whereas tertiary alcohols did neither. Although most of the experiments were performed with the green alga Chlamydomonas eugametos, the relevance for higher plants was demonstrated by showing that PLD in carnation petals could also be activated by mastoparan. The results indicate that PLD activation must be considered as a potential signal transduction mechanism in plants, just as in animals.
This paper references
Phosphatidatedependent protein phosphorylation
S. B. Boccklno (1991)
Synergistic activation of phos
B. Geny (1992)
Evidence for G
I. R. White (1993)
Signaling through phosphatidylcholine breakdown.
J. Exton (1990)
Lysophosphatidic acid activation of phosphatidylcholine-hydrolysing phospholipase D and actin polymerization by a pertussis toxin-sensitive mechanism.
Kwon Soo Ha (1994)
Lipid vesicles which can bind to protein kinase C and activate the enzyme in the presence of EGTA.
R. Epand (1992)
Characteristics of phospholipase C present in membranes of Xenopus laevis oocytes. Stimulation by phosphatidic acid.
G. Jacob (1993)
factors: 20-kDa guanine nucleotide-binding proteins. Methods Enzymol
T. Munnik (1994)
Chapter 16 - Generation and Attenuation of Lipid Second Messengers in Intracellular Signaling
W. J. V. Blitterswijk (1994)
Cell signalling through phospholipid breakdown
J. Exton (1991)
G proteins.
J. Hepler (1992)
Phosphatidic acid directly activates endothelial cell protein kinase C.
J. Stasek (1993)
Inositol trisphosphate and calcium signalling
M. Berridge (1993)
Cell signaling through phospholipid
J. H. Exton (1991)
Novel function of phosphatidylinositol 4,5-bisphosphate as a cofactor for brain membrane phospholipase D.
M. Liscovitch (1994)
Phospholipid signaling
N. Divecha (1995)
Phospholipase D catalyzes phospholipid metabolism in chemotactic peptide-stimulated HL-60 granulocytes.
J. Pai (1988)
The base exchange enzymes and phospholipase D of mammalian tissue.
J. Kanfer (1980)
Phosphatidic acid with medium-length fatty acyl chains synergistically stimulates phospholipase C with Ca2+ in rabbit platelets.
T. Hashizume (1992)
ARF signaling: A potential role for phospholipase D in membrane traffic
R. Kahn (1993)
A new phospholipid-splitting
D. J. Hanahan (1947)
Messenger molecules derived from membrane lipids.
J. Exton (1994)
Activation of cholera toxin by ADPribosylation
J. Moss (1994)
Inositol(1,4,5)trisphosphate production in plant cells: stimulation by the venom peptides, melittin and mastoparan.
B. Drøbak (1994)
Signal transduction and membrane traffic: The PITP/phosphoinositide connection
M. Liscovitch (1995)
Why do plants have phosphoinositides?
G. G. Coté (1994)
lnositol lipids in cell signaling
R. F. Irvine (1992)
Evidence for participation
L. Legendre (1992)
Gβγ interactions with PH domains and Ras-MAPK signaling pathways
James Inglese (1995)
The Effects of Mastoparan on the Carrot Cell Plasma Membrane Polyphosphoinositide Phospholipase C
M. H. Cho (1995)
The plant phosphoinositide system.
B. Drøbak (1992)
Regulation of G-protein activation by mastoparans and other cationic peptides.
E. Ross (1994)
Phosphatidic acid and diacylglycerol synergize in a cell-free system for activation of NADPH oxidase from human neutrophils.
D. Qualliotine-Mann (1993)
The role of cytosolic Ca2+
L. Gustavson (1994)
Signal transduction and membrane traffic: The PlTPlphosphoinositide connection
M. Liscovitch (1994)
Phosphatidylethanol biosynthesis in ethanol-exposed NG108-15 neuroblastoma X glioma hybrid cells. Evidence for activation of a phospholipase D phosphatidyl transferase activity by protein kinase C.
M. Liscovitch (1989)
Peroxides of Vanadate Induce Activation of Phospholipase D in HL-60 Cells
Sylvain BourgoinS (1992)
Phosphatidylethanol biosynthesis in ethanolexposed NG108-15 neuroblastoma X glioma hybrid cells
M. Liscovitch (1989)
Heterotrimeric C proteins: Organizers of transmembrane signals
E. Neer (1995)
Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues.
R. Irvine (1989)
[Phospholipase D].
T. Taki (1986)
G-protein-regulated phospholipases C, D and A2-mediated signalling in neutrophils.
S. Cockcroft (1992)
Plant Phosphoinositides and Intracellular Signaling
B. Drøbak (1993)
Rapid turnover of phosphatidylinositol 3-phosphate in the green alga Chlamydomonas eugametos: signs of a phosphatidylinositide 3-kinase signalling pathway in lower plants?
T. Munnik (1994)
Regulation of G protein activation by mastoparans
E. Ross (1994)
Phospholipid signaling. Cell80, Drdbak, B.K. (1992). The plant phosphoinositide system
N Divecha (1995)
Activation by thiol of the latent NAD glycohydrolase and ADP-ribosyltransferase activities of Bordetella pertussis toxin (islet-activating protein).
J. Moss (1983)
Regulation of interleukin‐2 responses by phosphatidic acid
E. Cano (1992)
Pertussis toxin-catalyzed ADP-ribosylation of G proteins.
D. J. Carty (1994)
G‐proteins in etiolated Avena seedlings Possible phytochrome regulation
L. C. Romero (1991)
The role of cytosolic Ca2+, protein kinase C, and protein kinase A in hormonal stimulation of phospholipase D in rat hepatocytes.
L. Gustavsson (1994)
Lipid second messengers
Mordechai Liscovitch (1994)
The formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D.
R. Dawson (1967)
Deflagellation of Chlamydomonas reinhardtii follows a rapid transitory accumulation of inositol 1,4,5-trisphosphate and requires Ca2+ entry
Y. Yueh (1993)
Ethanol and signal transduction in the liver
J. Hoek (1992)
lnositol phospholipid metabolism may trigger flagellar excission in Chlamydomonas
L. M. Quarmby (1992)
Phospholipase D: a downstream effector of ARF in granulocytes.
S. Cockcroft (1994)
Protein kinase C and lipid signaling for sustained cellular responses
Y. Nishizuka (1995)
Evidence that v-Src-induced phospholipase D activity is mediated by a G protein.
H. Jiang (1994)
Synthetic Peptides to Mimic the Role of GTP Binding Proteins in Membrane Traffic and Fusion
G. Law (1994)
Polyphosphoinositol lipids in Chlamydomonas eugametos gametes
J. Brederoo (1991)
lnitial events in phytochrome signaling: Still in the dark
T. D. Elich (1994)
A new phospholipide-splitting enzyme specific for the ester linkage between the nitrogenous base and the phosphoric acid grouping.
D. J. Hanahan (1947)
Cyclic GMP and calcium mediate phytochrome phototransduction
C. Bowler (1994)
A blue-light-activated
K. M. Warpeha (1991)
Crosstalk among multiple signal-activated phospholipases.
M. Liscovitch (1992)
Alcohols selectively stimulate phospholipase D‐mediated hydrolysis of phosphatidylethanolamine in NIH 3T3 cells
Z. Kiss (1989)
Centrin plays an essential role in microtubule severing during flagellar excision in Chlamydomonas reinhardtii
M. Sanders (1994)
C-protein-regulated phospholipases C, D and A2-mediated signalling in neutrophils
Shamshad Cockroft (1992)
Receptor-to-effector signaling through G proteins: Roles for βγ dimers as well as α subunits
L. Birnbaumer (1992)
Inositol Trisphosphate and Calcium Signaling
M. Berridge (1995)
Second messenger function of phosphatidic acid in platelet activation
M. Kroll (1989)
Cell signalling through phospholipid breakdown.
J. Exton (1991)
Cloning and expression of phosphatidylcholine-hydrolyzing phospholipase D from Ricinus communis L.
X. Wang (1994)
Ca2 +‐mobilizing hormones elicit phosphatidylethanol accumulation via phospholipase D activation
S. Bocckino (1987)
X. Wang (1993)
The regulation of phospholipase Cyl by phosphatidic acid . Assessment of kinetic parameters
G. A. Jones (1993)
Ethanol stimulates phospholipid 24559 - 24563 . 659 - 662 . 21403 - 21406 . turnover and inositol l , 4 , 5 - trisphosphate production in Chlamydomonas eugametos gametes
A. Musgrave (1992)
Evidence for participation of GTP-binding proteins in elicitation of the rapid oxidative burst in cultured soybean cells.
L. Legendre (1992)
Regulation of interleukin-2
E. Cano (1992)
Formation of phosphatidylethanol in rat brain by phospholipase D.
L. Gustavsson (1987)
Guanine-nucleotide- and adenine-nucleotide-dependent regulation of phospholipase D in electropermeabilized HL-60 granulocytes.
M. Xie (1991)
The regulation and cellular functions of phosphatidylcholine hydrolysis.
M. Billah (1990)
Phosphatidic acid is a specific activator of phosphatidylinositol-4-phosphate kinase.
Andreas Moritz (1992)
Signal transduction in the sexual life cycle of Chlamydomonas
L. M. Quarmby (1994)
A role for phospholipase D in control of mitogenesis.
M. Boarder (1994)
Arf proteins: the membrane traffic police?
A. Boman (1995)
Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells.
L. Legendre (1993)
lnositol phospholipid metabolism may trigger flagellar excission in Chlamydomonas reinhardtii
L. M. Quarmby (1992)
Ethanol stimulates phospholipid
A. Musgrave (1992)
Stimulation of phosphatidylinositol4,5-bisphosphate
S. Jackowski (1989)
Pertussis toxin-catalyzed ADP-ribosylation of G pro-163-224. 73-81
D J Carty (1994)
Py dimers as well as a subunits
L. Birnbaumer (1990)
Phospholipase D and cell signaling.
M. Billah (1993)
Transphosphatidylation by phospholipase D.
S. Yang (1967)
Activation of NADPH oxidase and phospholipase D in permeabilized human neutrophils. Correlation between oxidase activation and phosphatidic acid production.
S. A. Bauldry (1992)
Stimulation of phosphatidylinositol 4,5-bisphosphate phospholipase C activity by phosphatidic acid.
S. Jackowski (1989)
Calcium/calmodulin-dependent and -independent phytochrome signal transduction pathways
G. Neuhaus (1993)
Phosphatidic acid activation of protein kinase C-zeta overexpressed in COS cells: comparison with other protein kinase C isotypes and other acidic lipids.
C. Limatola (1994)
Increase in calcium triggers mating structure activation in Chlamydomonas eugametos.
F. Schuring (1990)
Identification, partial purification, and characterization of a novel phospholipid-dependent and fatty acid-activated protein kinase from human platelets.
W. Khan (1994)
Activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in IIC9 fibroblasts
Kwon Soo Ha (1993)
Modulation of the elicitation response in cultured french bean cells and its implication for the mechanism of signal transduction
G. Bolwell (1991)
Receptor-effector coupling by G proteins.
L. Birnbaumer (1990)
Inositol polyphosphate metabolism and inositol lipids in a green alga, Chlamydomonas eugametos.
R. Irvine (1992)
G proteins: transducers of receptor-generated signals.
A. Gilman (1987)
Occurrence of O-methylated sugars in surface glycoconjugates in Chlamydomonas eugametos
F. Schuring (2004)
Evidence for G-protein-linked receptors in higher plants: stimulation of GTP-gamma-S binding to membrane fractions by the mastoparan analogue mas 7
I. R. White (2004)
cium / calmodulin - dependent and - independent phytochrome signal transduction pathways
G. Neuhaus (1993)
Transphosphatidyla - tion by phospholipase D
S. F. Yang (1967)
Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii
L. Quarmby (1994)
Phosphatidylethanol Formation via Transphosphatidylation by Rat Brain Synaptosomal Phospholipase D
M. Kobayashi (1987)
Purification and characterization of the zeta isoform of protein kinase C from bovine kidney.
H. Nakanishi (1992)
Gonadotropin-releasing hormone activates phospholipase D in ovarian granulosa cells
M. Liscovitch (1989)
Activation of cholera toxin by ADP-ribosylation factors, 20-kDa guanine nucleotide-binding proteins.
J. Moss (1992)
First thoughts on lipid second messengers.
M. Roberts (1994)
Gonadotropin-releasing hormone activates phospholipase D in ovarian granulosa cells. Possible role in signal transduction.
M. Liscovitch (1989)
Activation and desensitization of phospholipase D in intact rat hepatocytes.
G. Moehren (1994)
Synergistic activation of phospholipase D by protein kinase C- and G-protein-mediated pathways in streptolysin O-permeabilized HL60 cells.
B. Geny (1992)
Cloning and expression of phosphatidylcholine-hydrolyzing
X. Wang (1994)
Phospha - tidic acid directly activates endothelial cell protein kinase C
J. E. Stasek (1993)
Peroxides of vanadate induce activation of phospholipase D in HL-60 cells. Role of tyrosine phosphorylation.
S. Bourgoin (1992)
Phosphatidylethanol formation via transphosphatidylation
M. Kobayashi (1987)
ADP-ribosylation factor, a small GTP-dependent regulatory protein, stimulates phospholipase D activity
H. A. Brown (1993)
Inositol 1,4,5-trisphosphate as fertilization signal in plants: testcase Chlamydomonas eugametos
A. Musgrave (2004)
Guanine nucleotide binding protein involvement in early steps of phytochrome-regulated gene expression.
L. C. Romero (1993)
Phosphatidate-dependent protein phosphorylation.
S. Bocckino (1991)
Tyrosine phosphorylation is involved in receptor coupling to phospholipase D but not phospholipase C in the human neutrophil.
I. Uings (1992)
A blue-light-activated GTP-binding protein in the plasma membranes of etiolated peas.
K. Warpeha (1991)

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S. Broughton (2011)
Characterization of five tomato phospholipase D cDNAs: Rapid and specific expression of LePLDß1 upon elicitation with xylanase Laxalt,
A. M. Laxalt ()
Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.
G. E. Scherer (2002)
Lipase activity in lipidomics – a hidden problem?
S. Furse (2013)
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M. Hartog (2008)
Multiple forms of phospholipase D in plants: the gene family, catalytic and regulatory properties, and cellular functions.
X. Wang (2000)
Chapter 14 – Molecular characteristics and cellular roles of guanine nucleotide binding proteins in plant cells
P. Millner (1999)
Heat shock-induced changes in lipid and protein metabolism in the endoplasmic reticulum of barley aleurone layers.
M. K. Johnston (2007)
Diacylglycerol pyrophosphate is a second messenger of abscisic acid signaling in Arabidopsis thaliana suspension cells.
Christine Zalejski (2005)
The role of phospholipase D in plant stress responses.
Bastiaan O. R. Bargmann (2006)
Intracellular Localization of Phospholipase D in Leaves and Seedling Tissues of Castor Bean
L. Xu (1996)
Phosphatidic Acid Directly Regulates PINOID-Dependent Phosphorylation and Activation of the PIN-FORMED2 Auxin Efflux Transporter in Response to Salt Stress
P. Wang (2018)
Factors involved in the rise of phosphoenolpyruvate carboxylase-kinase activity caused by salinity in sorghum leaves
J. Monreal (2013)
Heat shock response in photosynthetic organisms: membrane and lipid connections.
I. Horváth (2012)
Phosphoglycerolipids are master players in plant hormone signal transduction
Martin Janda (2013)
Phospholipase D antagonist 1-butanol inhibited the mobilization of triacylglycerol during seed germination in Arabidopsis
Yanxia Jia (2018)
Cell death signaling and morphology in chemical-treated tobacco BY-2 suspension cultured cells
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A. Tirajoh (2005)
1-Methylcyclopropene and CaCl 2 Treatments Affect Lipolytic Enzymes in Fresh-cut Watermelon Fruit
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Host factors involved in RNA replication of Dianthovirus
K. Hyodo (2014)
Activation of phospholipases by N-acetylchitooligosaccharide elicitor in suspension-cultured rice cells mediates reactive oxygen generation
T. Yamaguchi (2003)
Structure and function of heterotrimeric G proteins in plants.
Y. Fujisawa (2001)
Rice phospholipase D isoforms show differential cellular location and gene induction.
J. D. Mcgee (2003)
Identification and Characterization of a Novel Plant Phospholipase D That Requires Polyphosphoinositides and Submicromolar Calcium for Activity in Arabidopsis*
K. Pappan (1997)
Molecular analysis of phospholipase D
X. Wang (1997)
A role for G proteins in plant hormone signalling
R. Hooley (1999)
The surface organization of diacylglycerol pyrophosphate and its interaction with phosphatidic acid at the air-water interface.
Ana L Villasuso (2010)
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