Online citations, reference lists, and bibliographies.

Cleavage Of Nuclear DNA Into Oligonucleosomal Fragments During Cell Death Induced By Fungal Infection Or By Abiotic Treatments.

D. Ryerson, M. Heath
Published 1996 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
It is often claimed that programmed cell death (pcd) exists in plants and that a form of pcd known as the hypersensitive response is triggered as a defense mechanism by microbial pathogens. However, in contrast to animals, no feature in plants universally identifies or defines pcd. We have looked for a hallmark of pcd in animal cells, namely, DNA cleavage, in plant cells killed by infection with incompatible fungi or by abiotic means. We found that cell death triggered in intact leaves of two resistant cowpea cultivars by the cowpea rust fungus is accompanied by the cleavage of nuclear DNA into oligonucleosomal fragments (DNA laddering). Terminal deoxynucleotidyl transferase-mediated dUTP nick end in situ labeling of leaf sections showed that fungus-induced DNA cleavage occurred only in haustorium-containing cells and was detectable early in the degeneration process. Such cytologically detectable DNA cleavage was also observed in vascular tissue of infected and uninfected plants, but no DNA laddering was detected in the latter. DNA laddering was triggered by [greater than or equal to]100 mM KCN, regardless of cowpea cultivar, but not by physical cell disruption or by concentrations of H2O2, NaN3, CuSO4, or ZnCl2 that killed cowpea cells at a rate similar to that of ladder-inducing KCN concentrations. These and other results suggest that the hypersensitive response to microbial pathogens may involve a pcd with some of the characteristics of animal apoptosis and that DNA cleavage is a potential indicator of pcd in plants.
This paper references
highly resistant to its attack
Phytol
419-448. highly resistant to its attack
Phytol
10.1126/science.7878463
Mechanisms and genes of cellular suicide
H. Steller (1995)
Relations between Puccinia graminis and plants
E. C. Stakman (1915)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Gene expression during leaf senescence. New Stakman Relations between Puccinia graminis and plants
C M Smart (1915)
Molecular cloning : a laboratory manual. Cold Spring Harbor, NY
H. Sambrook (1989)
10.1111/j.1469-8137.1994.tb04243.x
Gene expression during leaf senescence
Catherine H M Smart (1994)
10.1139/b91-154
Cytological studies of the hypersensitive death of cowpea epidermal cells induced by basidiospore-derived infection by the cowpea rust fungus
C. Chen (1991)
10.1073/pnas.92.10.4158
Function of the oxidative burst in hypersensitive disease resistance.
R. Tenhaken (1995)
10.1016/0092-8674(94)90218-6
Arabidopsis mutants simulating disease resistance response
R. Dietrich (1994)
10.1105/tpc.7.1.29
Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump.
R. Mittler (1995)
ldentification and inhibition of the ICEICED-3 protease necessary for mammalian apoptosis Social controls on cell survival and cell death
D W Nicholson (1992)
10.1038/376037a0
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
D. Nicholson (1995)
Acomparison of fungal growth and plant responses in cowpea and bean cultivars inoculated with urediospores or basidiospores of the cowpea rustfungus
M.C. (1989)
10.1016/S0065-2296(08)60074-9
Cellular Interactions between Plants and Biotrophic Fungal Parasites
M. Heath (1997)
10.1105/tpc.6.7.917
Tracing Tracheary Element Development
R. Chasan (1994)
A comparison of the death induced by fungal invasion or toxic chemicals in cowpeaepidermal cells
S.L.F. Meyer (1988)
10.1104/pp.108.2.489
In Situ Detection of nDNA Fragmentation during the Differentiation of Tracheary Elements in Higher Plants
R. Mittler (1995)
10.1038/356397a0
Social controls on cell survival and cell death
M. Raff (1992)
10.1094/Phyto-62-27
Ultrastructure of Host and Nonhost Reactions to Cowpea Rust
M. Heath (1972)
10.1093/oxfordjournals.pcp.a078561
Oxidative Damage Caused by an Excess of Copper in Oat Leaves
C. Luna (1994)
10.1094/Phyto-74-1370
Relationship Between Heat-Induced Fungal Death and Plant Necrosis in Compatible and Incompatible Interactions Involving the Bean and Cowpea Rust Fungi
M. Heath (1984)
10.1139/b88-087
A comparison of the death induced by fungal invasion or toxic chemicals in cowpea epidermal cells. I: Cell death induced by heavy metal salts
S. L. Meyer (1988)
Laboratory techniques for demonstrating cell death
I. D. Bowen (1984)
10.1016/S0962-8924(00)89006-6
Apoptosis: lessons from in vitro systems.
W. Earnshaw (1995)
10.1016/0092-8674(94)90217-8
Programmed cell death in plants: A pathogen-triggered response activated coordinately with multiple defense functions
J. Greenberg (1994)
10.1105/tpc.7.11.1951
Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death.
R. Mittler (1995)
10.1139/b88-088
A comparison of the death induced by fungal invasion or toxic chemicals in cowpea epidermal cells. II. Responses induced by Erysiphe cichoracearum
S. L. Meyer (1988)
10.1038/bjc.1972.33
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
10.1080/09553009214551201
Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis.
R. Collins (1992)
10.1105/tpc.8.3.375
Apoptosis: A Functional Paradigm for Programmed Plant Cell Death Induced by a Host-Selective Phytotoxin and Invoked during Development.
H. Wang (1996)



This paper is referenced by
Programmed cell death in the leaves of the Arabidopsis spontaneous necrotic spots ( snsD ) mutant correlates with increased expression of the eukaryotic translation initiation factor eIF 4 B 2
G. Gaussand (2011)
10.1105/tpc.8.10.1793
Death Don't Have No Mercy: Cell Death Programs in Plant-Microbe Interactions.
J. Dangl (1996)
10.1023/A:1006333103342
Regulation of programmed cell death in maize endosperm by abscisic acid
T. E. Young (2004)
10.1007/s004250050429
Elicitor-induced cell death and phytoalexin synthesis in Daucus carota L.
W. Koch (1998)
10.1111/j.1364-3703.2008.00473.x
Plant programmed cell death: can't live with it; can't live without it.
Brett Williams (2008)
10.4067/S0717-34582002000100011
Application of rice (Oryza sativa L.) suspension culture in studying senescence in vitro (I). Single strand preferring nuclease activity
R. Hosseini (2002)
10.1007/s00425-003-1136-y
Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor
T. Hagihara (2004)
10.1371/journal.ppat.1002467
The Cysteine Rich Necrotrophic Effector SnTox1 Produced by Stagonospora nodorum Triggers Susceptibility of Wheat Lines Harboring Snn1
Z. Liu (2012)
Programmed cell death or apoptosis: Do animals and plants share anything in common
Nishawar Jan (2013)
10.1093/JXB/ERM239
The hypersensitive response; the centenary is upon us but how much do we know?
L. Mur (2008)
Improvement of forages to increase the efficiency of nutrient and energy use in temperate pastoral livestock systems
M. K. Theodorou (2008)
10.1270/JSBBS.54.347
Programmed Cell Death Detected in Interspecific Hybrids of Nicotiana repanda * N. tomentosiformis Expressing Hybrid Lethality
S. Kobori (2004)
ZEN1 Is a Key Enzyme in the Degradation of Nuclear DNA during Programmed Cell Death of Tracheary Elements
Hiroo Fukudaab (2002)
dod-1 , A Putative Programmed CellDeathSuppressor Gene in Rice
Yoshikazu Tanakai (2017)
10.1007/s004250000329
Apoptotic cell death induces temperature-sensitive lethality in hybrid seedlings and calli derived from the cross of Nicotiana suaveolens×N. tabacum
Tetsuya Yamada (2000)
10.1046/J.1432-1327.2000.01553.X
Independent pathways leading to apoptotic cell death, oxidative burst and defense gene expression in response to elicitin in tobacco cell suspension culture.
M. Sasabe (2000)
10.1186/s12870-016-0908-z
Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant
A. Bahieldin (2016)
10.1007/0-387-23266-4_5
The Hypersensitive Response in Plant Disease Resistance
N. Watanabe (2006)
Biologists ZEN 1 Is a Key Enzyme in the Degradation of Nuclear DNA during Programmed Cell Death of Tracheary Elements
J. Ito (2002)
10.1046/J.1365-313X.2000.00690.X
AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
P. Sánchez (2000)
10.1007/s11183-005-0018-5
Detection of apoptotic bodies in nickel-treated root apical cells of Allium cepa
L. Samadi (2005)
10.2216/i0031-8884-43-6-768.1
Apoptotic and necrotic stages of Symbiodinium (Dinophyceae) cell death activity: bleaching of soft and scleractinian corals
K. Strychar (2004)
10.1046/j.1365-2672.2002.01651.x
Salt stress induces programmed cell death in prokaryotic organism Anabaena
S-B Ning (2002)
10.1007/s10535-012-0286-9
Drought induced programmed cell death and associated changes in antioxidants, proteases, and lipid peroxidation in wheat leaves
A. Hameed (2012)
10.1023/A:1005868402827
Characterization of nuclease activities and DNA fragmentation induced upon hypersensitive response cell death and mechanical stress
R. Mittler (2004)
10.1093/jxb/err313
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
M. Greco (2012)
10.1046/J.1365-3040.2003.01090.X
Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells
P. Mlejnek (2003)
10.1094/PHYTO.2004.94.12.1295
Structural Modifications and Programmed Cell Death of Chili Pepper Fruit Related to Resistance Responses to Colletotrichum gloeosporioides Infection.
Kwang-Hyung Kim (2004)
Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides
C. Jing (2003)
10.1016/S0074-7696(02)18014-4
Three-dimensional progression of programmed death in the rice coleoptile.
N. Inada (2002)
10.1016/j.crvi.2016.02.004
Suppression of PCD-related genes affects salt tolerance in Arabidopsis.
A. Bahieldin (2016)
10.1016/B978-0-12-385851-1.00004-4
The Botanical Dance of Death: Programmed Cell Death in Plants
J. Kacprzyk (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar