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Cleavage Of Nuclear DNA Into Oligonucleosomal Fragments During Cell Death Induced By Fungal Infection Or By Abiotic Treatments.

D. Ryerson, M. Heath
Published 1996 · Biology, Medicine

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It is often claimed that programmed cell death (pcd) exists in plants and that a form of pcd known as the hypersensitive response is triggered as a defense mechanism by microbial pathogens. However, in contrast to animals, no feature in plants universally identifies or defines pcd. We have looked for a hallmark of pcd in animal cells, namely, DNA cleavage, in plant cells killed by infection with incompatible fungi or by abiotic means. We found that cell death triggered in intact leaves of two resistant cowpea cultivars by the cowpea rust fungus is accompanied by the cleavage of nuclear DNA into oligonucleosomal fragments (DNA laddering). Terminal deoxynucleotidyl transferase-mediated dUTP nick end in situ labeling of leaf sections showed that fungus-induced DNA cleavage occurred only in haustorium-containing cells and was detectable early in the degeneration process. Such cytologically detectable DNA cleavage was also observed in vascular tissue of infected and uninfected plants, but no DNA laddering was detected in the latter. DNA laddering was triggered by [greater than or equal to]100 mM KCN, regardless of cowpea cultivar, but not by physical cell disruption or by concentrations of H2O2, NaN3, CuSO4, or ZnCl2 that killed cowpea cells at a rate similar to that of ladder-inducing KCN concentrations. These and other results suggest that the hypersensitive response to microbial pathogens may involve a pcd with some of the characteristics of animal apoptosis and that DNA cleavage is a potential indicator of pcd in plants.
This paper references
highly resistant to its attack
419-448. highly resistant to its attack
Mechanisms and genes of cellular suicide
H. Steller (1995)
Relations between Puccinia graminis and plants
E. C. Stakman (1915)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Gene expression during leaf senescence. New Stakman Relations between Puccinia graminis and plants
C M Smart (1915)
Molecular cloning : a laboratory manual. Cold Spring Harbor, NY
H. Sambrook (1989)
Gene expression during leaf senescence
Catherine H M Smart (1994)
Cytological studies of the hypersensitive death of cowpea epidermal cells induced by basidiospore-derived infection by the cowpea rust fungus
C. Chen (1991)
Function of the oxidative burst in hypersensitive disease resistance.
R. Tenhaken (1995)
Arabidopsis mutants simulating disease resistance response
R. Dietrich (1994)
Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump.
R. Mittler (1995)
ldentification and inhibition of the ICEICED-3 protease necessary for mammalian apoptosis Social controls on cell survival and cell death
D W Nicholson (1992)
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
D. Nicholson (1995)
Acomparison of fungal growth and plant responses in cowpea and bean cultivars inoculated with urediospores or basidiospores of the cowpea rustfungus
M.C. (1989)
Cellular Interactions between Plants and Biotrophic Fungal Parasites
M. Heath (1997)
Tracing Tracheary Element Development
R. Chasan (1994)
A comparison of the death induced by fungal invasion or toxic chemicals in cowpeaepidermal cells
S.L.F. Meyer (1988)
In Situ Detection of nDNA Fragmentation during the Differentiation of Tracheary Elements in Higher Plants
R. Mittler (1995)
Social controls on cell survival and cell death
M. Raff (1992)
Ultrastructure of Host and Nonhost Reactions to Cowpea Rust
M. Heath (1972)
Oxidative Damage Caused by an Excess of Copper in Oat Leaves
C. Luna (1994)
Relationship Between Heat-Induced Fungal Death and Plant Necrosis in Compatible and Incompatible Interactions Involving the Bean and Cowpea Rust Fungi
M. Heath (1984)
A comparison of the death induced by fungal invasion or toxic chemicals in cowpea epidermal cells. I: Cell death induced by heavy metal salts
S. L. Meyer (1988)
Laboratory techniques for demonstrating cell death
I. D. Bowen (1984)
Apoptosis: lessons from in vitro systems.
W. Earnshaw (1995)
Programmed cell death in plants: A pathogen-triggered response activated coordinately with multiple defense functions
J. Greenberg (1994)
Identification, characterization, and purification of a tobacco endonuclease activity induced upon hypersensitive response cell death.
R. Mittler (1995)
A comparison of the death induced by fungal invasion or toxic chemicals in cowpea epidermal cells. II. Responses induced by Erysiphe cichoracearum
S. L. Meyer (1988)
Apoptosis: A Basic Biological Phenomenon with Wide-ranging Implications in Tissue Kinetics
J. Kerr (1972)
Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis.
R. Collins (1992)
Apoptosis: A Functional Paradigm for Programmed Plant Cell Death Induced by a Host-Selective Phytotoxin and Invoked during Development.
H. Wang (1996)

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Plant programmed cell death: can't live with it; can't live without it.
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M. Sasabe (2000)
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A. Bahieldin (2016)
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N. Watanabe (2006)
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J. Ito (2002)
AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
P. Sánchez (2000)
Detection of apoptotic bodies in nickel-treated root apical cells of Allium cepa
L. Samadi (2005)
Apoptotic and necrotic stages of Symbiodinium (Dinophyceae) cell death activity: bleaching of soft and scleractinian corals
K. Strychar (2004)
Salt stress induces programmed cell death in prokaryotic organism Anabaena
S-B Ning (2002)
Drought induced programmed cell death and associated changes in antioxidants, proteases, and lipid peroxidation in wheat leaves
A. Hameed (2012)
Characterization of nuclease activities and DNA fragmentation induced upon hypersensitive response cell death and mechanical stress
R. Mittler (2004)
In Posidonia oceanica cadmium induces changes in DNA methylation and chromatin patterning
M. Greco (2012)
Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells
P. Mlejnek (2003)
Structural Modifications and Programmed Cell Death of Chili Pepper Fruit Related to Resistance Responses to Colletotrichum gloeosporioides Infection.
Kwang-Hyung Kim (2004)
Programmed Cell Death During Secondary Xylem Differentiation in Eucommia ulmoides
C. Jing (2003)
Three-dimensional progression of programmed death in the rice coleoptile.
N. Inada (2002)
Suppression of PCD-related genes affects salt tolerance in Arabidopsis.
A. Bahieldin (2016)
The Botanical Dance of Death: Programmed Cell Death in Plants
J. Kacprzyk (2011)
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