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A Convenient And Versatile Hydroponic Cultivation System For Arabidopsis Thaliana

Hanna Norén, P. Svensson, B. Andersson, B. Andersson
Published 2004 · Biology

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A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics.
This paper references
Arabidopsis thaliana: a model plant for genome analysis.
D. Meinke (1998)
Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana
T. Nanjo (1999)
A method for isolating a high yield of Arabidopsis chloroplasts capable of efficient import of precursor proteins.
L. M. Fitzpatrick (2001)
Photochemical activity and components of membrane preparations from blue-green algae. I. Coexistence of two photosystems in relation to chlorophyll a and removal of phycocyanin.
D. Arnon (1974)
Auxiliary Photosynthetic Functions of Arabidopsis thaliana—Studies in vitro and in vivo
H. Norén (1999)
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
R. J. Porra (1989)
A novel method for growing Arabidopsis thaliana plants hydroponically
R. Arteca (2000)
Increase in the activity of phenylalanine ammonia-lyase during the non-photoperiodic induction of flowering in seedlings of morning glory (Pharbitis nil)
N. Hirai (1995)
A simple hydroponic culture method for the development of a highly viable root system in Arabidopsis thaliana.
T. Toda (1999)
Simple method of Arabidopsis thaliana cultivation in liquid nutrient medium
A. Siedlecka (2002)
Differential Regulation of Trichome Formation on the Adaxial and Abaxial Leaf Surfaces by Gibberellins and Photoperiod in Arabidopsis thaliana (L.) Heynh
J. Chien (1996)
Influence of Unsaturated Fatty Acids in Chloroplasts
P. Siegenthaler (1976)
A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana
M. Koornneef (2004)
Maximal Biomass of Arabidopsis thaliana Using a Simple, Low-Maintenance Hydroponic Method and Favorable Environmental Conditions
D. Gibeaut (1997)
Effects of sulfate concentrations on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis thaliana.
M. Hirai (1995)
Iron Nutrition Influence on Cadmium Accumulation by Arabidopsis thaliana (L.) Heynh.
K. D. Rodecap (1994)
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
The Arabidopsis Genome Initiative (2000)

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L. Bindschedler (2011)
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Shin-Ichiro Inoue (2017)
Adaptation of Arabidopsis Plants to Tropical Aeroponics Using Cool Root Zone Temperatures
Sandhya Srikanth (2017)
Profiling of secondary metabolites in root exudates of Arabidopsis thaliana.
Nadine Strehmel (2014)
Sustainable initiative of using cyanobacteria as a liquid fertilizer for hydroponic cultivation: A waste to wealth utilization.
Sharon Maria Jacob (2020)
Lysigenous Aerenchyma Formation in Arabidopsis Is Controlled by LESION SIMULATING DISEASE1[W][OA]
Per Mühlenbock (2007)
The Sink-Specific Plastidic Phosphate Transporter PHT4;2 Influences Starch Accumulation and Leaf Size in Arabidopsis1[W][OA]
S. Irigoyen (2011)
Beneficial bacteria and fungi in hydroponic systems: Types and characteristics of hydroponic food production methods
Seungjun Lee (2015)
Nutrient solutions for Arabidopsis thaliana: a study on nutrient solution composition in hydroponics systems
Sander H. van Delden (2020)
Cadmium uptake and distribution in Arabidopsis thaliana exposed to low chronic concentrations depends on plant growth
M. Dauthieu (2009)
Phosphorylation of Photosystem II Controls Functional Macroscopic Folding of Photosynthetic Membranes in Arabidopsis[C][W][OA]
R. Fristedt (2009)
Peptidyl-prolyl cis-trans Isomerases in the Chloroplast Thylakoid Lumen
A. Edvardsson (2007)
Identification, Expression, and Functional Analyses of a Thylakoid ATP/ADP Carrier from Arabidopsis*
Sophie Thuswaldner (2007)
High Light Induced Disassembly of Photosystem II Supercomplexes in Arabidopsis Requires STN7-Dependent Phosphorylation of CP29
R. Fristedt (2011)
Qualitative and quantitative modifications of root mitochondria during senescence of above-ground parts of Arabidopis thaliana.
D. D. Fanello (2017)
Gene expression profiling analysis of copper homeostasis in Arabidopsis thaliana.
Talía del Pozo (2010)
Internalization and Phytotoxic Effects of CuO Nanoparticles in Arabidopsis thaliana as Revealed by Fatty Acid Profiles.
J. Yuan (2016)
Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops
A. Margenot (2018)
Critical evaluation and statistical validation of a hydroponic culture system for Arabidopsis thaliana.
K. Smeets (2008)
From green to yellow : a leaf story
O. Keech (2007)
A “Nonsterile” Method for Selecting and Growing Arabidopsis thaliana Transformants (T2 Transgenic Lines) Resistant to Kanamycin
W. Xu (2008)
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
L. Bindschedler (2008)
Assay of photoinhibition and heat inhibition of photosystem II in higher plants.
Nobuyoshi Nijo (2011)
A Functional Genomics Approach to the Plant Soluble Pyrophosphatase Family
Z. N. Ergen (2006)
Tissue preparation using Arabidopsis.
Aimee M. Llewellyn (2012)
A Root-Expressed Magnesium Transporter of the MRS2/MGT Gene Family in Arabidopsis thaliana Allows for Growth in Low-Mg2+ Environments[W]
M. Gebert (2009)
Differential Expression and Localization of Early Light-Induced Proteins in Arabidopsis 1
M. Heddad (2006)
A femtomolar-range suicide germination stimulant for the parasitic plant Striga hermonthica
D. Uraguchi (2018)
MDR‐like ABC transporter AtPGP4 is involved in auxin‐mediated lateral root and root hair development
Diana Santelia (2005)
Triin Kollist (2005)
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