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Improved Fluorescence Staining Technique For Estimating Turnover Of The Human Stratum Corneum

L. Jansen, M. T. Hojyo-Tomoko, A. Kligman
Published 1974 · Medicine

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The replacement time of the human stratum corneum of fifteen body regions was determined by measuring the days required for a strongly substantive fluorescent marker, Dansyl chloride, to disappear. Tbe horny layer was stained by a 24‐h patch exposure to 5% Dansyl chloride (5‐dimethylamino‐ 1‐naphthalene‐sulphonyl chloride) in petrolatum.
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