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Effects Of Nitrogen And Phosphorus Deficiencies On Levels Of Carbohydrates, Respiratory Enzymes And Metabolites In Seedlings Of Tobacco And Their Response To Exogenous Sucrose

M. Paul, M. Stitt
Published 1993 · Biology

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A simple method of growing plants in agar was exploited to investigate the effect of long-term nitrogen (N) and phosphorus (P) deficiencies on respiratory metabolism and growth in shoots and roots of Nicotiana tabacum seedlings, and their interaction with exogenously supplied sucrose. Levels of hexose phosphates and 3-phosphoglyceric acid (3-PGA) were low in P-deficient shoots and roots and high in N-deficient shoots and roots. The ratio of hexose phosphates to 3-PGA and levels of fructose-2,6-bisphosphate were high in P-deficient plants and low in N-deficient plants. These data reflect differences in the way metabolism was perturbed, yet both deficiencies were associated with increased root growth relative to shoot growth, starch accumulation in the shoots, and soluble carbohydrate accumulation, especially hexoses, in the roots. Enzymes for sucrose degradation (sucrose synthase, acid and alkaline invertase) and glycolysis (phosphofructokinase, pyrophosphate-dependent phospho-fructokinase and pyruvate kinase) remained unaltered or declined in the shoots and roots. The accumulation of hexoses in roots of N- and P-deficient plants may result from maintenance of high invertase activities relative to sucrose synthase and glycolytic enzymes in the roots. The possibility that hexose accumulation may drive preferential root growth osmotically in N and P deficiencies is discussed. The addition of sucrose to roots to further investigate the interaction of carbohydrates with growth and allocation in low N and low P produced clear effects even though endogenous levels of soluble carbohydrate were already high in the nutrient-deficient plants. In complete nutrition, growth was stimulated, protein content particularly of the roots was increased and there was a preferential increase in activity of sucrose synthase in roots. At low P, enzyme activities in roots were increased, including sucrose synthase, and protein content increased, particularly in the roots, but there was no increase in growth. In N-deficient plants, exogenous sucrose led to decreased protein, Rubisco and chlorophyll content in shoots, in contrast to the other conditions, and a higher protein content and a general increase of catabolic enzyme activities and growth in the roots.
This paper references
10.1093/OXFORDJOURNALS.PCP.A077461
Effects of Light and Nitrogen Nutrition on the Organization of the Photosynthetic Apparatus in Spinach
I. Terashima (1988)
10.1104/PP.89.4.1117
Identification of actively filling sucrose sinks.
S. Sung (1989)
10.1104/PP.57.1.63
Pyrophosphorylases in Solanum tuberosum: I. Changes in ADP-Glucose and UDP-Glucose Pyrophosphorylase Activities Associated with Starch Biosynthesis during Tuberization, Maturation, and Storage of Potatoes.
J. Sowokinos (1976)
10.1104/PP.88.3.725
Alterations in leaf carbohydrate metabolism in response to nitrogen stress.
T. Rufty (1988)
10.1104/PP.98.3.1163
Sucrose Synthase in Wild Tomato, Lycopersicon chmielewskii, and Tomato Fruit Sink Strength.
J. Sun (1992)
10.1016/S0176-1617(11)80209-9
Changes in Photosynthesis/Respiration Ratio and Levels of Few Carbohydrates in Leaves of Nutrient Depleted Barley and Pea
Björn Thorsteinsson (1990)
10.1146/ANNUREV.PP.41.060190.001101
Fructose-2,6-Bisphosphate as a Regulatory Molecule in Plants
M. Stitt (1990)
10.1111/J.1365-313X.1991.00095.X
Transgenic tobacco plants expressing yeast‐derived invertase in either the cytosol, vacuole or apoplast: a powerful tool for studying sucrose metabolism and sink/source interactions
U. Sonnewald (1991)
10.1104/PP.90.3.820
Leaf Phosphate Status, Photosynthesis and Carbon Partitioning in Sugar Beet: II. Diurnal Changes in Sugar Phosphates, Adenylates, and Nicotinamide Nucleotides.
I. Rao (1989)
10.1104/PP.98.3.1133
Phosphorus Nutrition Influence on Starch and Sucrose Accumulation, and Activities of ADP-Glucose Pyrophosphorylase and Sucrose-Phosphate Synthase during the Grain Filling Period in Soybean.
S. Crafts-Brandner (1992)
10.1104/PP.92.4.963
Regulation of expression of carbon-assimilating enzymes by nitrogen in maize leaf.
B. Sugiharto (1990)
10.1104/PP.90.3.814
Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet: I. Changes in growth, gas exchange, and calvin cycle enzymes.
I. Rao (1989)
10.1104/PP.75.3.665
Partitioning of Nitrogen among Ribulose-1,5-bisphosphate Carboxylase/Oxygenase, Phosphoenolpyruvate Carboxylase, and Pyruvate Orthophosphate Dikinase as Related to Biomass Productivity in Maize Seedlings.
T. Sugiyama (1984)
10.1002/j.1460-2075.1990.tb07552.x
A feedback control element near the transcription start site of the maize Shrunken gene determines promoter activity.
C. Maas (1990)
10.1105/tpc.4.1.59
Sugar Levels Modulate Differential Expression of Maize Sucrose Synthase Genes.
K. Koch (1992)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1002/j.1460-2075.1990.tb07499.x
Expression of a yeast‐derived invertase in the cell wall of tobacco and Arabidopsis plants leads to accumulation of carbohydrate and inhibition of photosynthesis and strongly influences growth and phenotype of transgenic tobacco plants.
A. Schaewen (1990)
10.1139/B84-074
Alterations in internal partitioning of carbon in soybean plants in response to nitrogen stress.
T. Rufty (1984)
10.1093/OXFORDJOURNALS.AOB.A087575
A review of evidence on the control of shoot: root ratio
J. B. Wilson (1988)
10.1016/0005-2728(87)90143-5
Subcellular compartmentation of pyrophosphate and alkaline pyrophosphatase in leaves
Hendrik Weiner (1987)
10.1016/0168-9452(88)90136-7
Effects of phosphate deficiency on assimilate partitioning in barley seedlings
R. Sicher (1988)
10.1086/337859
Soluble Carbohydrate Allocation to Roots, Photosynthetic Rate of Leaves, and Nitrate Assimilation as Affected by Nitrogen Stress and Irradiance
L. Henry (1991)
10.1104/PP.93.4.1337
Growth of the Maize Primary Root at Low Water Potentials : II. Role of Growth and Deposition of Hexose and Potassium in Osmotic Adjustment.
R. Sharp (1990)
10.1104/PP.98.1.316
Diurnal starch accumulation and utilization in phosphorus-deficient soybean plants.
J. Qiu (1992)
10.1111/J.1399-3054.1987.TB04340.X
Carbohydrate partitioning, photosynthesis and growth in Lemna gibba G3. I: Effects of nitrogen limitation
Björon Thorsteinsson (1987)
10.1111/J.1365-313X.1993.00817.X
Regulation of the Expression of Rbcs and Other Photosynthetic Genes by Carbohydrates - a Mechanism for the Sink Regulation of Photosynthesis
A. Krapp (1993)
10.1104/PP.90.4.1275
Phosphate Starvation Inducible ;Bypasses' of Adenylate and Phosphate Dependent Glycolytic Enzymes in Brassica nigra Suspension Cells.
S. Duff (1989)
10.1071/PP9860221
Effects of Phosphorus Nutrition on Ribulose-1,5-Bisphosphate Carboxylase Activation, Photosynthetic Quantum Yield and Amounts of Some Calvin-Cycle Metabolites in Spinach Leaves
A. Brooks (1986)
10.1097/00010694-196704000-00001
RESPONSE OF PLANTS TO PHOSPHATE CONCENTRATION IN SOLUTION CULTURE: I. GROWTH AND PHOSPHORUS CONTENT
C. Asher (1967)
10.1071/PP9870059
Effects of Nitrogen Nutrition on Electron Transport Components and Photosynthesis in Spinach
J. R. Evans (1987)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1016/0076-6879(89)74035-0
Metabolite levels in specific cells and subcellular compartments of plant leaves
M. Stitt (1989)



This paper is referenced by
10.1016/S0167-4781(02)00502-X
Effects of phosphate deficiency and sugars on expression of rab18 in Arabidopsis: hexokinase-dependent and okadaic acid-sensitive transduction of the sugar signal.
I. Ciereszko (2002)
10.1104/pp.010058
Interactions of Nitrate and CO2 Enrichment on Growth, Carbohydrates, and Rubisco in Arabidopsis Starch Mutants. Significance of Starch and Hexose1
J. Sun (2002)
10.2134/ASASPECPUB61.C2
Plant Respiratory Responses to Elevated Carbon Dioxide Partial Pressure
J. Amthor (2015)
10.1007/s11120-017-0402-y
Significance of accumulation of the alarmone (p)ppGpp in chloroplasts for controlling photosynthesis and metabolite balance during nitrogen starvation in Arabidopsis
Rina Honoki (2017)
10.1071/FP06253
Phosphorus deficiency inhibits growth in parallel with photosynthesis in a C3 (Panicum laxum) but not two C4 (P. coloratum and Cenchrus ciliaris) grasses.
O. Ghannoum (2007)
10.1007/BF02942298
Sucrose regulates growth and activation of rubisco in tobacco leavesin vitro
K. Roh (2004)
In vitro and ex vitro potato plantlets (Solanum tuberosum) metabolic response to exogenously supplied sucrose : a metabolomic approach
A. Badr (2011)
10.1016/B978-044452975-6.50010-1
NUTRITION OF SUBSTRATE-GROWN PLANTS
A. Silber (2008)
10.1111/j.1365-3040.2010.02245.x
Acclimation to high CO2 in maize is related to water status and dependent on leaf rank.
Anneke Prins (2011)
10.1093/AOB/MCI049
Construction and maintenance of the optimal photosynthetic systems of the leaf, herbaceous plant and tree: an eco-developmental treatise.
I. Terashima (2005)
10.5539/JAS.V3N1P170
Yield Response of Uniculm Wheat (Triticum aestivum L.) to Early and Late Application of Nitrogen: Flag Leaf Development and Senescence
S. Kumari (2011)
10.1023/A:1017901817063
Efficient In Vitro Micropropagation and Regeneration of Humulus Lupulus on Low Sugar, Starch-Gelrite Media
I. Smýkalová (2004)
10.1016/0168-9452(96)04464-0
Expression of enzyme activities and transcripts required for nitrate assimilation in maize (Zea mays L. A188 × BMS) suspension cell cultures☆
M. Redinbaugh (1996)
10.1023/A:1005841119665
A novel gene whose expression in Medicago truncatula roots is suppressed in response to colonization by vesicular-arbuscular mycorrhizal (VAM) fungi and to phosphate nutrition
S. Burleigh (2004)
10.1023/A:1012415004676
Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation
D. Haisel (2004)
10.1046/J.1365-3040.1999.00386.X
The interaction between elevated carbon dioxide and nitrogen nutrition: the physiological and molecular background
M. Stitt (1999)
10.1093/OXFORDJOURNALS.PCP.A029057
Interaction between Nitrogen Deficit of a Plant and Nitrogen Content in the Old Leaves
K. Ono (1996)
10.1104/PP.119.2.445
Glycolytic flux is adjusted to nitrogenase activity in nodules of detopped and argon-treated alfalfa plants
Curioni (1999)
Inhibition of SNF1-related protein kinase1 by trehalose 6-phosphate and other metabolites and the interrelation with plant gorwth
Cátia Maria de Jesus Nunes (2015)
10.1007/S10163-018-00816-Y
By-products of bioenergy systems (anaerobic digestion and gasification) as sources of plant nutrients: scope of processed application and effect on soil and crop
S. Kataki (2019)
Perfil fisiológico e da expressão de transportadores de fosfato da cana-de-açúcar (Saccharum spp.) durante a simbiose com micorriza arbuscular
R. S. Almeida (2007)
10.3389/fpls.2011.00049
Exploring the Molecular and Metabolic Factors Contributing to the Adaptation of Maize Seedlings to Nitrate Limitation
A. El-kereamy (2011)
Improving yield potential in crops under elevated CO 2 : integrating the photosynthetic and nitrogen utilization
S. Seneweera (2012)
Genome-Wide Analysis of the Arabidopsis Leaf Transcriptome Reveals Interaction of Phosphate and Sugar Metabolism 1 [ W ]
R. Müller (2006)
10.1079/IVP2006748
Plant regeneration of Chorispora bungeana via somatic embryogenesis
J. Wang (2007)
10.1111/J.1365-3040.1996.TB00225.X
Phloem‐specific expression of pyrophosphatase inhibits long distance transport of carbohydrates and amino acids in tobacco plants
P. Geigenberger (1996)
10.1385/1-59259-959-1:131
Protocol to achieve photoautotrophic coconut plants cultured in vitro with improved performance ex vitro.
G. Fuentes (2006)
10.5483/BMBREP.2012.45.6.010
Arabidopsis SIZ1 positively regulates alternative respiratory bypass pathways.
B. Park (2012)
Regulation of glucose metabolism in the actinomycetes amycolatopsis methanolica and streptomyces coelicolor A3(2)
A. Alves (1997)
10.1016/J.ENVEXPBOT.2017.07.010
Effects of phosphorus availability on later stages of primary succession in Gongga Mountain glacier retreat area
Lei Yu (2017)
10.1007/s10535-009-0080-5
Effect of sucrose application, minerals, and irradiance on the in vitro growth of Cistus incanus seedlings and plantlets
D. Mills (2009)
10.1007/s004250050535
Phosphoenolpyruvate carboxylase in developing seeds of Vicia faba L.: gene expression and metabolic regulation
S. Golombek (1999)
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