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Distinct And Overlapping Roles Of Two Gibberellin 3-oxidases In Arabidopsis Development.
M. Mitchum, Shinjiro Yamaguchi, A. Hanada, Ayuko Kuwahara, Y. Yoshioka, Tomohiko Kato, S. Tabata, Y. Kamiya, T. Sun
Published 2006 · Biology, Medicine
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Gibberellin (GA) 3-oxidase, a class of 2-oxoglutarate-dependent dioxygenases, catalyzes the conversion of precursor GAs to their bioactive forms, thereby playing a direct role in determining the levels of bioactive GAs in plants. Gibberellin 3-oxidase in Arabidopsis is encoded by a multigene family consisting of at least four members, designated AtGA3ox1 to AtGA3ox4. It has yet to be investigated how each AtGA3ox gene contributes to optimizing bioactive GA levels during growth and development. Using quantitative real-time PCR analysis, we have shown that each AtGA3ox gene exhibits a unique organ-specific expression pattern, suggesting distinct developmental roles played by individual AtGA3ox members. To investigate the sites of synthesis of bioactive GA in plants, we generated transgenic Arabidopsis that carried AtGA3ox1-GUS and AtGA3ox2-GUS fusions. Comparisons of the GUS staining patterns of these plants with that of AtCPS-GUS from previous studies revealed the possible physical separation of the early and late stages of the GA pathway in roots. Phenotypic characterization and quantitative analysis of the endogenous GA content of ga3ox1 and ga3ox2 single and ga3ox1/ga3ox2 double mutants revealed distinct as well as overlapping roles of AtGA3ox1 and AtGA3ox2 in Arabidopsis development. Our results show that AtGA3ox1 and AtGA3ox2 are responsible for the synthesis of bioactive GAs during vegetative growth, but that they are dispensable for reproductive development. The stage-specific severe GA-deficient phenotypes of the ga3ox1/ga3ox2 mutant suggest that AtGA3ox3 and AtGA3ox4 are tightly regulated by developmental cues; AtGA3ox3 and AtGA3ox4 are not upregulated to compensate for GA deficiency during vegetative growth of the double mutant.
This paper references
Evidence for the Translocation of Gibberellin A3 and Gibberellin-Like Substances in Grafts between Normal, Dwarf1 and Dwarf5 Seedlings of Zea mays L.
M. Katsumi (1983)
Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants.
M. Talón (1990)
Cellular Growth in Roots of a Gibberellin-Deficient Mutant of Tomato (Lycopersicon esculentum Mill.) and its Wild-type
P. Barlow (1991)
Gibberellin concentration and transport in genetic lines of pea : effects of grafting.
W. Proebsting (1992)
The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.
T. Sun (1994)
Isolation of the Arabidopsis GA4 locus.
H. Chiang (1995)
Effects of Low Irradiance Stress on Gibberellin Levels in Pea Seedlings
H. Gawrońska (1995)
Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis
A. Phillips (1995)
Developmental regulation of the gibberellin biosynthetic gene GA1 in Arabidopsis thaliana.
A. Silverstone (1997)
Gibberellins and Stem Growth in Arabidopsis thaliana (Effects of Photoperiod on Expression of the GA4 and GA5 Loci)
Y. L. Xu (1997)
Mendel's stem length gene (Le) encodes a gibberellin 3 beta-hydroxylase.
D. R. Lester (1997)
Mendel's dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins.
D. N. Martin (1997)
Phytochrome Regulation and Differential Expression of Gibberellin 3β-Hydroxylase Genes in Germinating Arabidopsis Seeds
Shinjiro Yamaguchi (1998)
The GA2 locus of Arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis.
S. Yamaguchi (1998)
Function and substrate specificity of the gibberellin 3beta-hydroxylase encoded by the Arabidopsis GA4 gene.
J. Williams (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
The gene encoding tobacco gibberellin 3beta-hydroxylase is expressed at the site of GA action during stem elongation and flower organ development.
H. Itoh (1999)
Regulation of gibberellin biosynthesis genes during flower and early fruit development of tomato.
M. Rebers (1999)
Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation.
S. Thomas (1999)
Gibberellin biosynthesis: its regulation by endogenous and environmental signals.
S. Yamaguchi (2000)
Gibberellin metabolism: new insights revealed by the genes.
P. Hedden (2000)
Gibberellin biosynthesis mutations and root development in pea.
J. Yaxley (2001)
Gibberellin Biosynthesis in Plants and Fungi: A Case of Convergent Evolution?
P. Hedden (2001)
Cloning and functional analysis of two gibberellin 3β-hydroxylase genes that are differently expressed during the growth of rice
H. Itoh (2001)
Repressing a Repressor
A. Silverstone (2001)
KNOX homeodomain protein directly suppresses the expression of a gibberellin biosynthetic gene in the tobacco shoot apical meristem.
T. Sakamoto (2001)
Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination.
S. Yamaguchi (2002)
The Gibberellin Pathway Mediates KNOTTED1-Type Homeobox Function in Plants with Different Body Plans
Angela Hay (2002)
Gibberellin signaling: biosynthesis, catabolism, and response pathways.
N. Olszewski (2002)
Auxin promotes Arabidopsis root growth by modulating gibberellin response
Xiangdong Fu (2003)
Where do gibberellin biosynthesis and gibberellin signaling occur in rice plants?
Miyuki Kaneko (2003)
Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana
J. Alonso (2003)
The redistribution of protein sulfur in transgenic rice expressing a gene for a foreign, sulfur-rich protein.
N. Hagan (2003)
ATM to the Rescue
N. Eckardt (2003)
Gibberellin Biosynthesis and Response during Arabidopsis Seed Germination Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.011650.
Mikihiro Ogawa (2003)
Gibberellins regulate seed germination in tomato by endosperm weakening: a study with gibberellin-deficient mutants
S. Groot (1987)
Activation of Gibberellin Biosynthesis and Response Pathways by Low Temperature during Imbibition of Arabidopsis thaliana Seeds On-line version contains Web-only data.
Y. Yamauchi (2004)
Effects of gibberellins, darkness and osmotica on endosperm rupture and class I β-1,3-glucanase induction in tobacco seed germination
G. Leubner-Metzger (1996)
An Overview of Gibberellin Metabolism Enzyme Genes and Their Related Mutants in Rice1[w]
T. Sakamoto (2004)
Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh.
M. Koornneef (1980)
DELLA Proteins and Gibberellin-Regulated Seed Germination and Floral Development in Arabidopsis1[w]
Ludmila Tyler (2004)
Transcriptional program controlled by the floral homeotic gene AGAMOUS during early organogenesis
C. Gómez-Mena (2005)
Maturation of the Ground Tissue of the Root Is Regulated by Gibberellin and SCARECROW and Requires SHORT-ROOT1[w]
A. Paquette (2005)
Assaying chimeric genes in plants: The GUS gene fusion system
R. Jefferson (1987)
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T. Lange (2020)
An Updated Overview on the Regulation of Seed Germination
Gerardo Carrera-Castaño (2020)
An ABA-GA bistable switch can account for natural variation in the variability of Arabidopsis seed germination time
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M. Groszmann (2020)
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H. Yoshida (2020)
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