Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Regulation Of Glyoxysomal Enzyme Expression In Maize

A. Tsaftaris, J. G. Scandalios
Published 1981 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The activity levels of three glyoxysomal enzymes (catalase, isocitric lyase, and malate synthase) were measured in the scutellum following germination of the inbred lines W64A, R6-67, and A16. In W64A, as in most maize lines examined, germination was accompanied by a rapid and synchronous increase in the activities of all three enzymes, and reached a peak at about day 4 and declined thereafter. In R6-67, catalase activity continues to increase past day 4 and reaches its highest activity level on later days. In A16, catalase activity is very low due to the lack of expression of the Cat2 gene. Despite these significant differences in catalase expression, the levels of the other two glyoxysomal enzymes did not differ in these inbred lines. Artificial inhibition of catalase in W64A by exogenous application of 10–4 M aminotriazole did not inhibit germination, nor did it alter the levels of the other two glyoxysomal enzymes. Similarly, application of 10–4 M itaconate to W64A seeds inhibited the appearance of isocitric lyase, but did not inhibit germination or alter the levels of malate synthase or catalase. Comparative cell fractionation and immunological studies were conducted with W64A and A16 and their microbodies were observed under the electron microscope. Cell fractionation studies were also conducted with W64A seeds germinated in the presence of aminotriazole or itaconate. Thus, our results suggest that the expression of these three glyoxysomal enzymes is not regulated coordinately in the maize scutellum.
This paper references
10.1104/PP.64.2.228
Enzyme Profiles in Seedling Development and the Effect of Itaconate, an Isocitrate Lyase-directed Reagent.
F. Khan (1979)
10.1083/JCB.56.2.500
THE DEVELOPMENT OF MICROBODIES AND PEROXISOMAL ENZYMES IN GREENING BEAN LEAVES
P. J. Gruber (1973)
10.1104/PP.43.4.660
Evidence for de novo synthesis of isocitratase and malate synthesis in germinating peanut cotyledons.
C. P. Longo (1968)
10.5694/j.1326-5377.1956.tb56775.x
Methods of Biochemical Analysis
D. Glick (1956)
10.1083/JCB.46.3.435
CYTOCHEMICAL AND DEVELOPMENTAL CHANGES IN MICROBODIES (GLYOXYSOMES) AND RELATED ORGANELLES OF CASTOR BEAN ENDOSPERM
E. Vigil (1970)
10.1146/ANNUREV.PP.22.060171.000401
Microbodies-Peroxisomes and Glyoxysomes
N. E. Tolbert (1971)
10.1104/PP.62.1.141
Control of Enzyme Activities in Cotton Cotyledons during Maturation and Germination: II. Glyoxysomal Enzyme Development in Embryos.
J. Choinski (1978)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1073/PNAS.77.9.5360
Genetic regulation of the catalase developmental program in maize scutellum: Identification of a temporal regulatory gene.
J. G. Scandalios (1980)
10.1016/s0021-9258(19)50881-x
A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.
R. Beers (1952)
10.1042/BJ0740339
Irreversible reaction of 3-amino-1:2:4-triazole and related inhibitors with the protein of catalase.
E. Margoliash (1960)
10.1083/JCB.44.1.94
DEVELOPMENTAL STUDIES ON GLYOXYSOMES IN RICINUS ENDOSPERM
B. Gerhardt (1970)
10.1016/S0006-291X(67)80007-X
Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm.
R. Breidenbach (1967)
10.1073/PNAS.68.7.1402
Turnover of genetically defined catalase isozymes in maize.
P. Quail (1971)
10.1104/PP.43.4.653
De Novo Synthesis of Isocitritase in Peanut (Arachis hypogaea L.) Cotyledons.
A. Gientka-Rychter (1968)
10.1002/9780470110188.CH13
Spectrophotometric assay of cytochrome c oxidase.
L. Smith (1955)
10.1093/OXFORDJOURNALS.JHERED.A108450
Subcellular Localization of Catalase Variants Coded by Two Genetic Loci During Maize Development
J. G. Scandalios (1974)
10.1016/B978-0-12-620980-8.50007-8
Control of Gene Expression and Enzyme Differentiation
J. G. Scandalios (1979)
10.1177/17.6.425
INTRACELLULAR LOCALIZATION OF CATALASE (PEROXIDATIC) ACTIVITY IN PLANT MICROBODIES
E. Vigil (1969)
10.1002/DVG.1020010308
Evaluation of a catalase inhibitor in the developmental regulation of maize catalase
A. Tsaftaris (1979)
10.1146/ANNUREV.PP.30.060179.001111
Microbodies in Higher Plants
H. Beevers (1979)
10.1111/j.1749-6632.1968.tb11896.x
GENETIC CONTROL OF MULTIPLE MOLECULAR FORMS OF CATALASE IN MAIZE *
J. G. Scandalios (1968)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar