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Purification And Further Properties Of Single-strand-specific Nuclease From Aspergillus Oryzae.

V. Vogt
Published 1973 · Biology, Medicine

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A simple scheme has been devised for the purification of Aspergillus nuclease S1 from commercially available crude amylase powder. Five purification steps yield 90% pure nuclease with an overall yield of 27%. The enzyme is shown to be absolutely specific for single-stranded nucleic acids. It degrades both DNA and RNA. The nuclease exhibits a sharp pH optimum at pH 4.2 and is active in low concentrations of dodecylsulfate. Aspergillus nuclease S1 is a metalloprotein of molecular weight 32000. The utility of this nuclease for measuring annealing of labeled DNA is discussed.
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