Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Effect Of Cd-containing Wood Ash On The Microflora Of Coniferous Forest Humus.

Fritze, Perkiömäki, Saarela, Katainen, Tikka, Yrjälä, Karp, Haimi, Romantschuk
Published 2000 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
The use of wood ash in forestry has been questioned because the cadmium (Cd) concentration of ash, which varies between 1 and 20 mg kg(-1) ash, exceeds the level allowed for fertilizers (3 mg kg(-1)) used in agriculture. To investigate the combined and separated effects of Cd and ash on the forest humus microflora, pumice or wood ash, spiked with a water-soluble (CdCl(2)) or -insoluble (CdO) form of Cd at three levels (0, 400 and 1000 mg kg(-1)), were applied at a fertilization level of 5000 kg ha(-1) in a laboratory microcosm study. The trial consisted of 60 microcosms (five replications per treatment), which were incubated in darkness at +20 degrees C and a constant relative air humidity of 60%. After two months the humus in the microcosms was sampled. Analyses of CO(2) evolution to measure the overall microbial activity and of phospholipid fatty acid (PLFA) pattern to measure microbial community structure were performed. The substrate-use patterns of Biolog EcoPlates were analyzed as a measure of bacterial functionality. Finally the bacterial (3)H-thymidine incorporation in the presence of different concentrations of Cd and the number of colony forming units (cfu) of bacteria on nutrient agar in the presence of 0, 5 and 20 mg Cd l(-1) agar were applied to measure Cd tolerance. The use of pumice (pH of humus under the pumice 4.0) did not induce any changes in the above variables compared to two untreated microcosms (humus pH 3.9). Pumice was therefore used to distribute the Cd evenly over the humus surface in order to estimate the possible effect of Cd without ash (pH of humus under the ash 7.0). The application of ash increased the microbial activity, changed the PLFA and substrate-use patterns and increased cfu compared to the humus under pumice. The form and level of Cd in the ash had no further effect on this result. In the humus under pumice the level, but not the form of Cd decreased the microbial activity and changed the PLFA pattern compared to the unspiked pumice. None of the treatments induced bacterial tolerance to Cd. Ash thus protected the humus microflora from the harmful effects of Cd.
This paper references
A New Set of Substrates Proposed for Community Characterization in Environmental Samples
H. Insam (1997)
Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis
Å. Frostegård (1993)
E. Bååth (1995)
Multiple heavy metal tolerance of soil bacterial communities and its measurement by a thymidine incorporation technique.
M. Díaz-Raviña (1994)
Straw compost and bioremediated soil as inocula for the bioremediation of chlorophenol-contaminated soil.
M. Laine (1996)
Phospholipid Fatty Acid Composition and Heavy Metal Tolerance of Soil Microbial Communities along Two Heavy Metal-Polluted Gradients in Coniferous Forests.
T. Pennanen (1996)
Analysis of BIOLOG GN Substrate Utilization Patterns by Microbial Communities
K. Smalla (1998)
Phospholipid Fatty Acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals.
A. Frostegård (1993)
Clear-cutting and prescribed burning in coniferous forest: Comparison of effects on soil fungal and total microbial biomass, respiration activity and nitrification
J. Pietikäinen (1995)
Growth rate and response of bacterial communities to pH in limed and ash treated forest soils
E. Bååth (1994)
Lipids in Bacterial Taxonomy - A Taxonomist's View
Lechevalier Mp (1977)
Measurement of heavy metal tolerance of soil bacteria using thymidine incorporation into bacteria extracted after homogenization-centrifugation
E. Bååth (1992)
Soil Bacterial Biomass, Activity, Phospholipid Fatty Acid Pattern, and pH Tolerance in an Area Polluted with Alkaline Dust Deposition.
E. Bååth (1992)
Chemical methods in bacterial systematics
M. Goodfellow (1985)
Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization.
J. Garland (1991)
Effects of ash fertilization and prescribed burning on macronutrient, heavy metal, sulphur and 137Cs concentrations in lingonberries (Vaccinium vitis-idaea)
T. Levula (2000)
Chemotaxonomic significance of fatty acid composition in the genus Mortierella (Zygomycetes, Mortierellaceae)
Norihide Amano (1992)
Cadmium contamination of wood ash and fire-treated coniferous humus: Effect on soil respiration
H. Fritze (1995)

This paper is referenced by
Wood Ash use in forestry
R. Pitman (2004)
Using community trait-distributions to assign microbial responses to pH changes and Cd in forest soils treated with wood ash
Carla Cruz-Paredes (2017)
Growth of saprotrophic fungi and bacteria in soil.
J. Rousk (2011)
Organic residues - a resource for arable soils
M. Odlare (2005)
Microbial immobilization of cadmium released from CdO in the soil
E. Kurek (2004)
Characterization of the forest humus microbial community in a heavy metal polluted area
M. Niklińska (2005)
Effects on Motile Factors and Cell Growth of Euglena gracilis After Exposure to Wood Ash Solution; Assessment of Toxicity, Nutrient Availability and pH-Dependency
K. Andreas (2005)
Effects of wood ash on the growth of known strains of Bacillus subtilis
T. R. Omodara (2014)
Impact of Zinc and Cadmium on the Microbial Community in Soils
P. Walker (2008)
Bacteria in a wood fungal disease: characterization of bacterial communities in wood tissues of esca-foliar symptomatic and asymptomatic grapevines
E. Bruez (2015)
Short and long-term effects of wood ash on the boreal forest humus microbial community
J. Perkiömäki (2002)
Wood Ash Induced pH Changes Strongly Affect Soil Bacterial Numbers and Community Composition
Toke Bang-Andreasen (2017)
Bacterial communitystructureandactivity in di ¡ erentCd-treated forest soils
A. Lazzaro (2006)
Does simulated acid rain increase the leaching of cadmium from wood ash to toxic levels to coniferous forest humus microbes?
J. Perkiömäki (2003)
Use of wood ash and anaerobic sludge for grassland fertilization: Effects on plants and microbes
H. Insam (2009)
The relative importance of the bacterial pathway and soil inorganic nitrogen increase across an extreme wood‐ash application gradient
Mette Vestergård (2018)
Community level physiological profile response to plant residue additions in Antarctic soils
E. Malosso (2005)
Effect of wood ash recycling and liquid fertilisation on the fine roots of Norway spruce
M. Genenger (2001)
In situ bioremediation through mulching of soil polluted by a copper-nickel smelter.
O. Kiikkilä (2001)
Integrated evaluation of the ecotoxicological risk of using sewage sludges in agriculture and in soil restoration
Tiago Natal da Luz (2011)
Labile carbon alleviates wood ash effects on soil fauna
J. K. Nieminen (2008)
Microbial biodiversity in a wooded riparian zone specificallydesigned for enhancing denitrification process
M. Rahman (2011)
Effect of Wood Ash and Compost Application on Nitrogen Transformations and Availability in Soil-Plant Systems
M. Abbasi (2013)
Microbial communities in drinking water systems analysed by lipid biomarkers
M. Keinänen (2003)
Methanogen Communities in a Drained Bog: Effect of Ash Fertilization
P. Galand (2003)
Cadmium in upland forests after vitality fertilization with wood ash—a summary of soil microbiological studies into the potential risk of cadmium release
J. Perkiömäki (2004)
Biological effects of wood ash application to forest and aquatic ecosystems.
K. Aronsson (2004)
Wood ash fertilization alters the forest humus Archaea community
K. Yrjälä (2004)
Does the harvest of logging residues and wood ash application affect the mobilization and bioavailability of trace metals
B. Olsson (2017)
Harmonising conflicts between science, regulation, perception and environmental impact: the case of soil conditioners from bioenergy.
M. J. Riding (2015)
Wood ash as a forest soil amendment: The role of boiler and soil type on soil property response
Stephanie C. Pugliese (2014)
Recycling of Biomass Ashes: Current Technologies and Future Research Needs
B. Knapp (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar