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Survival, Regeneration And Functional Recovery Of Motoneurons In Adult Rats By Reimplantation Of Ventral Root Following Spinal Root Avulsion

H. Gu, H. Chai, J. Zhang, Z. Yao, L. Zhou, Wai-Man Wong, Iain Bruce, Wu-tian Wu
Published 2004 · Medicine

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We investigated the functional recovery of motoneurons after reimplanting an avulsed ventral root in a rat model of traction injury. The eighth cervical root (C8) was avulsed by controlled traction and immediately reimplanted to the spinal cord. Spinal nerves from neighbouring segments (C5, C6, C7 and T1) were ligated and cut. After 12 or 20 weeks, the survival, regeneration and functional recovery of spinal motoneurons were evaluated by Nissl staining, retrograde labelling of motoneurons, NOS histochemistry, histological examination of muscle and nerve–muscle junction, electromyography and behavioural observation. In the control animals, about 14% or 11% of spinal motoneurons survived 12 or 20 weeks postinjury, respectively. By contrast, in animals with ventral root reimplantation, 62% and 55% of motoneurons survived at 12 or 20 weeks postinjury, respectively. Retrograde labelling and histological examination indicated that about 90% of the surviving motoneurons in the C8 segment regenerated axons into the reimplanted ventral root. Staining the muscles with silver and cholinesterase revealed new motor endplates in the reinnervated muscle. Functionally significant electromyographic responses in flexor digitorum superficialis and flexor carpi radialis were observed in experimental animals; however, the average latency of the motor action potentials was greater than normal control. The grasping test showed functional recovery of finger flexors and median nerve. In conclusion, our results indicate that spinal motoneurons can regenerate axons through reimplanted roots and reinnervate muscles to recover partial function.
This paper references
The cellular response to nerve injury. 3. The effect of repeated crush injuries.
Thomas Pk (1970)
Regeneration by supernumerary axons with synaptic terminals in spinal motoneurons of cats
L. Havton (1987)
How to correctly match 175,000 neurites: two postulates for a quick solution.
L. de Medinaceli (1987)
Alterations in Synaptic Strength Preceding Axon Withdrawal
H. Colman (1997)
Direct cortico-motoneuronal synaptic contacts are present in the adult rat cervical spinal cord and are first established at postnatal day 7
M. H. Curfs (1996)
Sprouting and regression of neuromuscular synapses in partially denervated mammalian muscles.
M. C. Brown (1978)
Activity‐dependent and ‐independent synaptic interactions during reinnervation of partially denervated rat muscle.
R. Ribchester (1988)
Synaptic segregation at the developing neuromuscular junction.
W. Gan (1998)
Neuron death in vertebrate development: in vitro methods.
P. G. Clarke (1995)
Bridging grafts and transient nerve growth factor infusions promote long-term central nervous system neuronal rescue and partial functional recovery.
M. Tuszynski (1995)
Injuries of the brachial plexus and neighboring peripheral nerves in vertebral fractures and other trauma of the cervical spine
Narakas Ao (1987)
A light and electron microscopic study of intracellularly HRP‐labeled lumbar motoneurons after intramedullary axotomy in the adult cat
H. Lindå (1992)
Neurotization procedures for brachial plexus injuries.
D. Chuang (1995)
How to correctly match 175,000 neurites: two postulates for a quick solution.
Luis de Medmaceli (1987)
Survival and regeneration of motoneurons in adult rats by reimplantation of ventral root following spinal root avulsion
H. Chai (2000)
Ideal intraspinal implantation site for the repair of ventral root avulsion after brachial plexus injury in humans. A preliminary anatomical study
H. Fournier (2001)
Chapter 13 Neuron Death in Vertebrate Development: In Vivo Methods
P. G. Clarke (1995)
Reimplantation of ventral rootlets into the cervical spinal cord after their avulsion: an anterior surgical approach
C. Hoffmann (1993)
The Rat Brain in Stereotaxic Coordinates
L. Swanson (1984)
The grasping test: a simple behavioral method for objective quantitative assessment of peripheral nerve regeneration in the rat
J. Bertelli (1995)
Motoneurons reinnervate skeletal muscle after ventral root implantation into the spinal cord of the cat
S. Cullheim (1989)
Brachial plexus repair by peripheral nerve grafts directly into the spinal cord in rats. Behavioral and anatomical evidence of functional recovery.
J. Bertelli (1994)
Implantation of PNS Graft Inhibits the Induction of Neuronal Nitric Oxide Synthase and Enhances the Survival of Spinal Motoneurons Following Root Avulsion
W. Wu (1994)
Implantation of PNS graft inhibits the induction of nitric oxide synthase and enhances the survival of spinal motoneurons due to root avulsion
W Wu (1994)
Experimental strategies to promote axonal regeneration after traumatic central nervous system injury
C. Stichel (1998)
Is it possible to predict the outcome of peripheral nerve injuries? A probability model based on prospects for regenerating neurites.
L. de Medinaceli (1987)
Reinnervation of avulsed and reimplanted ventral rootlets in the cervical spinal cord of the cat.
C. Hoffmann (1996)
Effects of Insulin-like Growth Factor-1 in Motor Nerve Regeneration After Nerve Transection and Repair vs. Nerve Crushing Injury in the Rat
B. Lutz (1999)
Reorganization of descending motor tracts in the rat spinal cord
O. Raineteau (2002)
The cellular response to nerve injury. 3. The effect of repeated crush injuries.
P. K. Thomas (1970)
Direct neurotization of severely damaged denervated muscles.
G. Brunelli (1980)
‘Dendraxons’ in regenerating motoneurons in the cat: do dendrites generate new axons after central axotomy?
H. Lindå (1985)
Restoration of shoulder abduction by nerve transfer in avulsed brachial plexus injury: evaluation of 99 patients with various nerve transfers.
D. Chuang (1995)
Lateral approach of the dog brachial plexus for ventral root reimplantation
P. Moissonnier (1998)
[Injuries of the brachial plexus and neighboring peripheral nerves in vertebral fractures and other trauma of the cervical spine].
A. Narakas (1987)
Spinal cord implantation of avulsed ventral roots in primates; correlation between restored motor function and morphology
R. Hallin (1999)

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Co-expression of GAP-43 and nNOS in avulsed motoneurons and their potential role for motoneuron regeneration.
Q. Yuan (2010)
At-level neuropathic pain is induced by lumbosacral ventral root avulsion injury and ameliorated by root reimplantation into the spinal cord
A. Bigbee (2007)
Limited BDNF contributes to the failure of injury to skin afferents to produce a neuropathic pain condition
Li-Jun Zhou (2010)
Age-related reexpression of p75 in axotomized motoneurons
Q. Yuan (2006)
Delayed riluzole treatment is able to rescue injured rat spinal motoneurons
A. Nógrádi (2007)
Spinal root avulsion and repair
Tak-Ho Chu (2019)
Reactive changes in dorsal roots and dorsal root ganglia after C7 dorsal rhizotomy and ventral root avulsion/replantation in rabbits
N. Schlegel (2007)
A spatio-temporal analysis of motoneuron survival, axonal regeneration and neurotrophic factor expression after lumbar ventral root avulsion and implantation
R. Eggers (2010)
Comparison of Different In Vivo Animal Models of Brachial Plexus Avulsion and Its Application in Pain Study
Hang Xian (2020)
Sigma receptor agonist 2-(4-morpholinethyl)1 phenylcyclohexanecarboxylate (Pre084) increases GDNF and BiP expression and promotes neuroprotection after root avulsion injury.
C. Penas (2011)
Cytoskeletal and activity-related changes in spinal motoneurons after root avulsion.
C. Penas (2009)
Microtubule stabilization promoted axonal regeneration and functional recovery after spinal root avulsion
H. Li (2017)
l-Theanine and NEP1-40 promote nerve regeneration and functional recovery after brachial plexus root avulsion.
W. Guo (2019)
Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats.
H. Su (2009)
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W. Guo (2019)
Differentiation of Pre- and Postganglionic Nerve Injury Using MRI of the Spinal Cord
A. Karalija (2016)
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T. Hoang (2006)
Survival, regeneration and functional recovery of motoneurons after delayed reimplantation of avulsed spinal root in adult rat
Huaiyu Gu (2005)
Berberine enhances L1 expression and axonal remyelination in rats after brachial plexus root avulsion
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Antisense oligos to neuronal nitric oxide synthase aggravate motoneuron death induced by spinal root avulsion in adult rat
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