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Characterization Of A Taxol‐resistant Human Small‐cell Lung Cancer Cell Line

S. Ohta, K. Nishio, N. Kubota, T. Ohmori, Y. Funayama, T. Ohira, H. Nakajima, M. Adachi, N. Saijo
Published 1994 · Biology, Medicine

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Taxol is a novel anticancer agent with activity against a broad range of tumors. It has a unique ability to stabilize polymerized tubulin into microtubule bundles within the cell. We have established a taxol‐resistant human small‐cell lung cancer cell line (H69/Txl) by exposing H69 cells to stepwise increases in taxol concentration. The resistance of H69/Txl cells to taxol was 4.7‐fold that of the original H69 cells: the IC50 values for H69 and H69/Txl were 113.7 ± 56.54 nM and 538.7 ± 214.7 nM by the tetrazolium dye assay, respectively. Removal of the drug from the medium resulted in a 38% decrease in the growth rate of H69/Txl as compared with that in the presence of 30 nM taxol, suggesting that the growth of H69/Txl was partially dependent on taxol. H69/Txl showed higher sensitivity to vinca alkaloids such as vindesine, vincristine and vinblastine than the parental H69. There was no significant difference in intracellular [3H]taxol content between H69 and H69/Txl cells. No MDR‐1 mRNA was detected in H69/Txl by the reverse transcription polymerase chain reaction. There was no significant difference of total and polymerized tubulin content between H69 and H69/Txl cells. Altered mobility of one of the α‐tubulin isoforms in H69/Txl was revealed by using isoelectric focusing and Western blotting with anti‐α‐tubulin antibody. In H69, two α‐tubulin isoforms were observed, whereas three were evident in H69/Txl, two of them comigrating with the isoforms of H69 and the other being more acidic. We observed the increased acetylation of α‐tubulin in H69/Txl cells as compared with that in H69 cells. The acetylation of α‐tubulin may be responsible for the taxol resistance and/or taxol‐dependent growth of H69/Txl.
This paper references
10.1073/PNAS.69.8.2283
Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate.
B. Eipper (1972)
10.1111/J.1432-1033.1975.TB02435.X
Incorporation of L-tyrosine, L-phenylalanine and L-3,4-dihydroxyphenylalanine as single units into rat brain tubulin.
C. Arce (1975)
10.1016/0092-8674(79)90061-8
Mutants of chinese hamster ovary (cho) cells with altered colcemid-binding affinity
V. Ling (1979)
10.1073/PNAS.76.9.4350
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
H. Towbin (1979)
10.1073/PNAS.77.3.1561
Taxol stabilizes microtubules in mouse fibroblast cells.
P. Schiff (1980)
10.1016/0092-8674(80)90231-7
CHO mutants resistant to colchicine, colcemid or griseofulvin have an altered β-tubulin
F. Cabral (1980)
10.1016/0092-8674(81)90270-1
Evidence that microtubule depolymerization early in the cell cycle is sufficient to initiate DNA synthesis
K. Crossin (1981)
10.1083/JCB.97.1.258
Chlamydomonas alpha-tubulin is posttranslationally modified in the flagella during flagellar assembly
S. W. L'hernault (1983)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
Reduced drug accumulation in multiply drug-resistant human KB carcinoma cell lines.
A. Fojo (1985)
A phosphoglycoprotein associated with taxol resistance in J774.2 cells.
S. Roy (1985)
10.1083/JCB.101.6.2081
Alpha-tubulin acetylase activity in isolated Chlamydomonas flagella
K. Greer (1985)
10.1016/0003-2697(86)90205-8
Rapid and effective transfer of integral membrane proteins from isoelectric focusing gels to nitrocellulose membranes.
S. Matthäi (1986)
10.1016/0003-2697(87)90021-2
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
10.1073/PNAS.85.15.5698
Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
E. Kawasaki (1988)
10.1093/JNCI/81.15.1144
MDR1 gene expression in lung cancer.
S. Lai (1989)
10.7326/0003-4819-111-4-273
Taxol: a unique antineoplastic agent with significant activity in advanced ovarian epithelial neoplasms.
W. Mcguire (1989)
10.1096/fasebj.3.14.2574119
P‐glycoprotein: multidrug‐resistance and a superfamily of membrane‐associated transport proteins
P. Juranka (1989)
10.1073/PNAS.87.18.7160
Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.
K. E. Noonan (1990)
10.1016/0022-1759(91)90248-E
Quantitation of cellular tubulin in microtubules and tubulin pools by a competitive ELISA.
D. Thrower (1991)
10.1093/JNCI/83.24.1797
Phase II trial of taxol, an active drug in the treatment of metastatic breast cancer.
F. Holmes (1991)
10.1073/pnas.88.7.2820
Direct photoaffinity labeling of tubulin with colchicine.
J. Wolff (1991)
10.1016/s0021-9258(19)67891-9
Resistance to antimitotic drugs in Chinese hamster ovary cells correlates with changes in the level of polymerized tubulin.
A. Minotti (1991)
10.1093/JNCI/84.10.785
Direct photoaffinity labeling of tubulin with taxol.
S. Rao (1992)
10.1247/CSF.17.139
Inhibitory effect of taxol, a microtubule stabilizing agent, on induction of DNA synthesis is dependent upon cell lines and growth factors.
K. Tsuji (1992)
10.1016/0003-2697(92)90358-E
Quantification of gene expression over a wide range by the polymerase chain reaction.
T. Kinoshita (1992)



This paper is referenced by
10.3390/biomedicines9050500
Paclitaxel-Based Chemotherapy Targeting Cancer Stem Cells from Mono- to Combination Therapy
Hend M. Nawara (2021)
10.1016/j.drup.2021.100754
Therapeutic strategies to overcome taxane resistance in cancer.
Tuyelee Das (2021)
10.2174/0929867326666191016113631
Recent Advances in Elucidating Paclitaxel Resistance Mechanisms in Non-small Cell Lung Cancer and Strategies to Overcome Drug Resistance.
H. Cui (2020)
10.1038/s41419-019-2180-2
Hsa_circ_0002483 inhibited the progression and enhanced the Taxol sensitivity of non-small cell lung cancer by targeting miR-182-5p
X. Li (2019)
10.1371/journal.pone.0117523
High-Affinity Accumulation of a Maytansinoid in Cells via Weak Tubulin Interaction
V. Goldmacher (2015)
10.1517/17530059.2013.826645
Advances in personalized therapy for lung cancer.
Eric S Kim (2013)
10.1016/j.mito.2012.10.013
In Vitro assessment of the utility of stearyl triphenyl phosphonium modified liposomes in overcoming the resistance of ovarian carcinoma Ovcar-3 cells to paclitaxel.
Melani A Solomon (2013)
10.1007/978-1-60761-524-8_15
Lung Cancer Resistance to Chemotherapy
D. J. Stewart (2010)
Nek4 Status Differentially Alters Sensitivity to Microtubule Poisons
J. Doles (2010)
Nouvelles fonctions de p21Cip1 dans la dynamique cytosquelettique des cellules épithéliales mammaires humaines
B. Bouchet (2010)
Identification and Characterization of Modulators of Chemotherapeutic Response in Mouse Models of Cancer
J. Doles (2010)
10.1016/j.critrevonc.2009.11.006
Tumor and host factors that may limit efficacy of chemotherapy in non-small cell and small cell lung cancer.
David J Stewart (2010)
10.1158/0008-5472.CAN-09-2113
Nek4 status differentially alters sensitivity to distinct microtubule poisons.
J. Doles (2010)
10.1007/978-1-59745-336-3_14
Mechanisms of Resistance to Drugs That Interfere with Microtubule Assembly
F. Cabral (2008)
10.1002/cmdc.200600308
Paclitaxel And Docetaxel Resistance: Molecular Mechanisms and Development of New Generation Taxanes
E. Galletti (2007)
Antiproliferating activity of the mitotic inhibitor pironetin against vindesine- and paclitaxel-resistant human small cell lung cancer H69 cells.
M. Yoshida (2007)
10.1007/s00280-007-0502-z
Preclinical antitumor activity of the oral platinum analog satraplatin
K. Wosikowski (2007)
10.1038/sj.bjc.6603936
Paclitaxel resistance in untransformed human mammary epithelial cells is associated with an aneuploidy-prone phenotype
B. Bouchet (2007)
10.1158/1535-7163.MCT-06-0277
Bcl-2 down-regulation and tubulin subtype composition are involved in resistance of ovarian cancer cells to vinflunine
M. Estève (2006)
10.1007/978-1-59745-035-5_18
Resistance To Taxanes
L. Greenberger (2006)
10.1517/17425255.2.5.753
Therapeutic resistance in lung cancer
A. Nadkar (2006)
10.1007/BF01240315
In vitro and in vivo modulation by rhizoxin of non-P-glycoprotein-mediated vindesine resistance
H. Arioka (2005)
10.2165/00003088-200544040-00002
Role of Cytochrome P450 Activity in the Fate of Anticancer Agents and in Drug Resistance
B. Rochat (2005)
10.1097/00001813-200411000-00003
Peptide-vector strategy bypasses P-glycoprotein efflux, and enhances brain transport and solubility of paclitaxel
E. Blanc (2004)
10.1016/J.BBRC.2003.12.001
Chromosome 13q12 encoded Rho GTPase activating protein suppresses growth of breast carcinoma cells, and yeast two-hybrid screen shows its interaction with several proteins.
G. M. Nagaraja (2004)
10.1038/nrc1317
Microtubules as a target for anticancer drugs
M. Jordan (2004)
Induction of a multifactorial resistance phenotype by high paclitaxel selective pressure in a human ovarian carcinoma cell line.
S. Violini (2004)
Resistance to microtubule-targeted cytotoxins in a K562 leukemia cell variant associated with altered tubulin expression and polymerization.
C. Dumontet (2004)
10.1038/sj.onc.1206934
Mechanisms of Taxol resistance related to microtubules
G. Orr (2003)
10.1007/s10120-003-0235-6
Absence of beta-tubulin gene mutation in gastric carcinoma
N. Urano (2003)
10.1111/j.1349-7006.2003.tb01465.x
DJ‐927, a novel oral taxane, overcomes P‐glycoprotein‐mediated multidrug resistance in vitro and in vivo
M. Shionoya (2003)
10.1007/s00432-003-0516-9
In vivo establishment and characterization of a paclitaxel-resistant human ovarian cancer cell line showing enhanced growth properties and drug-resistance only in vivo
K. Okugawa (2003)
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