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Leukotrienes B4, C4 And D4 Stimulate DNA Synthesis In Cultured Human Epidermal Keratinocytes

K. Kragballe, L. Desjarlais, J. Voorhees
Published 1985 · Biology, Medicine

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Leukotrienes in psoriatic skin lesions are potent mediators of inflammation. We have studied the capacity of leukotrienes to stimulate the DNA synthesis of cultured human epidermal keratinocytes. At concentrations ranging from 10−12 to 10−8 M, LTB4 produced a 100% increase of DNA synthesis determined both as the incorporation of [3H] thymidine and as the labelling index. In comparison, LTB4 had no effect on the DNA synthesis of dermal fibroblast cultures. 5S, 12S‐LTB4 and 5S, 12S‐all‐trans‐LTB4 did not change the DNA synthesis of keratinocytes, but the effect of LTB4 was abolished in the presence of 5S, 12S‐all‐trans HLTB4. Being less potent than LTB4 the peptidoleukotrienes (LTC4, LTD4) also stimulated keratinocyte DNA synthesis. The effect of the peptidoleukotrienes, but not of LTB4, was antagonized by EPL 55712. These results show that leukotrienes B4, C4 and D4 exert potent and stereospecific mitogenic effects on cultured human keratinocytes. The presence of these arachidonic acid metabolites in psoriatic skin lesions may be pertinent to both inflammation and aberrant epidermal growth in psoriasis.
This paper references
Leukotriene B4 induces m^itro fibroblast chemotaxis. J«Mr«a/tf//wuesn;?anW Dermatology
Mensing (1984)
Lcukotriene B . ,like material in stale of psoriatic skin lesion
R. D. R. CAMP
Leukotrienes: positive signals for regulation of y-interferon production. >>wrNa/ of Immunology
H M Johnson (1984)
10.1111/j.1476-5381.1980.tb07037.x
THE EFFECTS OF SOME ANTIRHEUMATIC DRUGS ON AN in vitro MODEL OF HUMAN POLYMORPHONUCLEAR LEUCOCYTE CHEMOKINESIS
M. Smith (1980)
Increased concentrations of nonesterifiedarachidonic acid, 12-L-hydroxy-5,8,io,i4-eicosatetraenoic acid, prostaglandin Ej, prostaglandin F^ in epidermis of psoriasis
Hammarstrom (1975)
B.,-like material in stale of psoriatic skin lesion
10.1111/J.1748-1716.1980.TB06676.X
Biological profile of leukotrienes C4 and D4.
P. Hedqvist (1980)
The release of LTC 4like material from the involved lesional skin in psoriasis
S. D. BRAIN
Inhibiton of leukotriene C and D h\o%yn&ie?.i's
S. HAMMARSTROM (1980)
Enhancement of 5-lipoxygenase activity on soluble preparations of human psoriatic plaque preparation
V. A. ZiBOH (1983)
10.1016/B978-0-08-029775-0.50072-9
Leukotrienes: mediators of immediate hypersensitivity reactions and inflammation.
B. Samuelsson (1983)
The release of LTC4-like material from the involved lesional skin in psoriasis
(1984)
Leukotrienes: positive signals for regulation of gamma-interferon production.
H. Johnson (1984)
10.1111/1523-1747.EP12540609
Cyclic AMP, glucocorticoid, and retinoid modulation of in vitro keratinocyte growth.
C. Marcelo (1983)
Stimulation of human leukocyte degranulation by leukotriene B.i and its lu-oxidized metabolites Leukotrime B, a potent chemokinetic and aggregating substance from polymorphonuclear leukocytes
Feinmark (1980)
Inhibition of leukotriene C and leukotriene D biosynthesis.
L. Orning (1980)
Complement receptor enhancement and chemotaxis of human neutrophils and eosinophils by leukotrienes and other lipoxygenase products.
L. Nagy (1982)
10.1038/NEWBIO245215A0
Selective inhibitor of slow reacting substance of anaphylaxis.
J. Augstein (1973)
Leukotrienes are potent constrictors
DAHLEN (1980)
The synthesis of leukotriene B ^iike material by cultured human keratinocytes
D. BRAINS. (1982)
10.1016/0006-291X(82)90622-2
Leukotriene B4 is a complete secretagogue in human neutrophils: a kinetic analysis.
C. Serhan (1982)
Isolationandgrowthof adult human epidermal keratinocytes. Jflurwti/o/7«ue5n
S C Liu (1978)
10.1111/1523-1747.EP12546943
Isolation and growth of adult human epidermal keratinocytes in cell culture.
S. Liu (1978)
10.1111/J.1748-1716.1980.TB06696.X
Leukotriene B4: a highly potent and stereospecific factor stimulating migration of polymorphonuclear leukocytes.
C. Malmsten (1980)
10.1172/JCI109904
Role of oxygen in antibody-dependent cytotoxicity mediated by monocytes and neutrophils.
N. Borregaard (1980)
The effect of leukotriene C, and Dj on cutaneous blood flow in humans
H Bisgaard (1982)
Leukotrienes: positive signals for regulation of y-interferon production. >>wrNa/ of Immunology
Johnson (1984)
10.1083/JCB.79.2.356
Stratification, specialization, and proliferation of primary keratinocyte cultures. Evidence of a functioning in vitro epidermal cell system
C. Marcelo (1978)
10.1111/1523-1747.EP12531738
Local Effects of Synthetic Leukotrienes (LTC4, LTD4, and LTB4) in Human Skin
N. Soter (1983)
10.1038/286264A0
Leukotriene B, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes
A. Ford-Hutchinson (1980)
10.1007/springerreference_38916
Leukotriene B4.
M. Smith (1981)
Specific binding of leukotriene B4 to receptors on human polymorphonuclear leukocytes.
D. W. Goldman (1982)
Biological profile of Ieukotrienes C 4 and D 4
P. Hedqvist (1980)
Production of intraepidermal
CAMP (1984)
The effect of some antirheumatic drugs on an in vitro mode! of human poiymorphonuclear leucocyte chemokinesis
M.J.H. SMITH (1980)
10.1016/0090-6980(82)90124-1
The effect of leucotriene C4 and D4 on cutaneous blood flow in humans
H. Bisgaard (1982)
10.1111/1523-1747.EP12258678
Leukotriene B4 induces in vitro fibroblast chemotaxis.
H. Mensing (1984)
10.1136/BMJ.285.6350.1241
Benoxaprofen: effect on cutaneous lesions in psoriasis.
B. Allen (1982)
Effects of free fatty acids on the growth of normal and neoplastic rat mammary epithelial cells.
M. Wicha (1979)
The synthesis of leukotriene B^-iike material by cultured human keratinocytes
Brain S D Camp (1982)
Leukotriene B 4 induces m ^ itro fibroblast chemotaxis
H. M E N S I N G (1984)
10.1111/j.1476-5381.1984.tb10148.x
Leukotriene B4‐like material in scale of psoriatic skin lesions
S. Brain (1984)
Increased concentrations of nonesterifiedarachidonic acid, 12-L-hydroxy-5,8,io,i4-eicosatetraenoic acid, prostaglandin Ej, prostaglandin F^ in epidermis of psoriasis
S Hammarstrom (1975)
10.1111/1523-1747.EP12259945
Production of intraepidermal microabscesses by topical application of leukotriene B4.
R. Camp (1984)
10.1038/288484A0
Leukotrienes are potent constrictors of human bronchi
Sven-Erik Dahlén (1980)
10.1001/ARCHDERM.1983.01650310010002
Benoxaprofen improves psoriasis. A double-blind study.
K. Kragballe (1983)
10.1136/bmj.285.6356.1741-b
Benoxaprofen: effect on cutaneous lesions in psoriasis
T. Kingston (1982)
Isolationandgrowthof adult human epidermal keratinocytes. Jflurwti/o/7«ue5n;?atiiie Dermatology
S C Liu (1978)
Leukotrime B , a potent chemokinetic and aggregating substance from polymorphonuclear leukocytes
A. W. FoRD-HuTCHiNSON (1980)
Isolationandgrowthof adult human epidermal keratinocytes
S. C. Liu (1978)
10.1073/PNAS.78.7.4579
Contractile activities of structural analogs of leukotrienes C and D: role of the polar substituents.
R. Lewis (1981)
10.1073/PNAS.72.12.5130
Increased concentrations of nonesterified arachidonic acid, 12L-hydroxy-5,8,10,14-eicosatetraenoic acid, prostaglandin E2, and prostaglandin F2alpha in epidermis of psoriasis.
S. Hammarstroem (1975)
10.1016/0014-5793(81)81233-1
Stimulation of human leukocyte degranulation by leukotriene B4 and its ω‐oxidized metabolites
S. Feinmark (1981)
Contractile properties of leukotrienes C and D: roie of the polar substituents
Lewis (1981)
The effect of some antirheumatic drugs on an in vitro mode! of human poiymorphonuclear leucocyte chemokinesis
Smith (1980)
The effect of leukotriene C , and D j on cutaneous blood flow in humans
H. BiSGAARD (1982)
Leukotriene B4 induces m^itro fibroblast chemotaxis. J«Mr«a/tf//wuesn;?anW Dermatology
H Mensing (1984)
Contractile properties of leukotrienes C and D: roie of the polar substituents
R. A. LEWIS (1981)



This paper is referenced by
10.1111/1523-1747.EP12616151
Cultured human melanocytes from black and white donors have different sunlight and ultraviolet A radiation sensitivities.
J. Yohn (1992)
10.1056/NEJM199306243282504
Effect of dietary supplementation with very-long-chain n-3 fatty acids in patients with psoriasis.
E. Søyland (1993)
10.1007/BF00455162
Increased aggregation and arachidonic acid transformation by psoriatic platelets: evidence that platelet-derived 12-hydroxy-eicosatetraenoic acid increases keratinocyte DNA synthesis in vitro
K. Kragballe (2004)
10.1038/jid.2009.155
Leukotriene B(4) mediates sphingosylphosphorylcholine-induced itch-associated responses in mouse skin.
T. Andoh (2009)
10.1111/j.1699-0463.1988.tb00917.x
Changes in lipoxygenase products from synovial fluid in carragheenan induced arthritis in dogs
T. Herlin (1988)
10.1016/S0190-9622(86)70004-2
Topical application of nonsteroidal anti-inflammatory drugs prevents vehicle-induced improvement of psoriasis.
C. Ellis (1986)
10.1016/1040-8428(96)00205-3
Biology and pathophysiology of leukotrienes.
C. Denzlinger (1996)
10.1111/j.1600-0625.1995.tb00225.x
Incorporation of 15‐hydroxyeicosatrienoic acid in specific phospholipids of cultured human keratinocytes and psoriatic plaques
J. Heitmann (1995)
10.1111/j.1365-2133.1988.tb03218.x
Characterization of specific leukotriene C4 binding sites on cultured human keratinocytes
A. Muller (1988)
10.1016/j.clindermatol.2007.11.002
Targeting leukocyte recruitment in the treatment of psoriasis.
Yi-Yang Yvonne Li (2008)
10.1007/978-3-642-75865-2_5
The Arachidonic Acid Cascade and Erythropoiesis
B. Beckman (1992)
10.3109/02713689108996339
Identification of 12(S)-hydroxyeicosatetraenoic acid in the young rat lens.
T. Lysz (1991)
10.1111/j.1365-2133.1985.tb04852.x
Increased DNA synthesis of uninvolved psoriatic epidermis is maintained in vitro
K. Kragballe (1985)
10.1016/B978-0-12-198875-3.50011-6
5 – Inhibitors of Fatty Acid-Derived Mediators
D. Hay (1994)
10.1016/0021-9150(94)05413-D
Modulation of rat vascular smooth muscle cell (VSMC) proliferation by cysteinyl leukotriene D4: a role for mediation of interleukin 1.
E. Porreca (1995)
Pathophysiological Role of Leukotrienes in Dermatological DiseasesTherapeutic Implications
B. Wedi (2001)
10.1016/S0306-3623(96)00075-4
Antipsoriatic anthrones: aspects of oxygen radical formation, challenges and prospects.
K. Müller (1996)
10.1001/ARCHDERM.1986.01660200049012
Selective decrease of 15-hydroxyeicosatetraenoic acid (15-HETE) formation in uninvolved psoriatic dermis.
K. Kragballe (1986)
10.3109/08923978809014400
The role of arachidonic acid metabolism in IL-3-induced proliferation.
B. Barton (1988)
10.1111/j.1346-8138.1988.tb03695.x
Low‐molecular‐weight Leukocyte Chemotactic Factors in Psoriatic Scales: Contribution of Lipid‐soluble Factors to the Chemotactic Activity
H. Takematsu (1988)
10.1016/0923-1811(93)90100-4
Growth inhibitory effect of eicosapentaenoic acid on cultured human epidermal keratinocytes.
R. Riku (1993)
10.1007/978-1-4615-3874-5_47
Mitogenic Effects of Eicosanoids in Human Airway Epithelial Cells
G. Leikauf (1991)
10.1056/NEJM199009063231006
Leukotrienes and other products of the 5-lipoxygenase pathway. Biochemistry and relation to pathobiology in human diseases.
R. Lewis (1990)
10.1007/978-1-4684-5700-1_3
Lipoxygenase products and their functions in glomeruli.
R. Ardaillou (1989)
10.1007/BF00387598
Human epidermis transforms exogenous leukotriene A4 into peptide leukotrienes: possible role in transcellular metabolism
L. Iversen (2004)
10.1111/1523-1747.EP12456487
Effects of substance P and substance K on the growth of cultured keratinocytes.
T. Tanaka (1988)
10.1016/j.det.2018.11.001
What's New in Psoriasis.
S. von Csiky-Sessoms (2019)
10.1016/J.PROSTAGLANDINS.2007.01.005
Pharmacotherapy of diseases mediated by 5-lipoxygenase pathway eicosanoids.
P. Rubin (2007)
10.1016/0145-2126(94)90053-1
Leukotrienes and lipoxins--new potential performers in the regulation of human myelopoiesis.
L. Stenke (1994)
10.1111/j.1365-2133.1988.tb01772.x
Low‐dose cyclosporin A in severe psoriasis. A double‐blind study
T. Joost (1988)
10.1002/DDR.430130206
Psoriasis: Hyperproliferative/inflammatory skin disorder
V. Ziboh (1988)
10.1007/978-1-4615-2071-9_5
Omega-3s and Immune/Inflammatory Responses
J. Nettleton (1995)
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