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Spontaneous Programmed Death (apoptosis) Of B‐chronic Lymphocytic Leukaemia Cells Following Their Culture In Vitro

R. Collins, L. A. Verschuer, B. Harmon, R. Prentice, J. Pope, J. Kerr
Published 1989 · Biology, Medicine

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Summary When B‐cell lymphocytic leukaemia (B‐CLL) cells derived from peripheral blood were cultured in vitro, a substantial proportion of them spontaneously died by apoptosis. This type of cell death is morphologically and biochemically distinct from necrosis and has previously been found to occur under physiologic and certain pathologic conditions where cell deletion appears controlled and biologically meaningful. By 30 h of culture, approximately 20% of the unfractionated B‐CLL cells were affected. There was no significant difference in the incidence of apoptosis in T‐cell depleted and undepleted cultures or when either autologous or normal human serum was used. Furthermore, seeding densities of 2 × 106 and 5 × 105 cells/ml resulted in a similar incidence of apoptosis, indicating that cell density was unlikely to be a contributing factor in producing the death. The finding that B‐CLL cells spontaneously die in vitro has at least two important implications. Firstly, previous work relating to some of the functions of B‐CLL cells and their interactions with T cells may require re‐evaluation. Secondly, an understanding of the mechanisms involved in the induction of apoptosis in this disease may have therapeutic consequences.
This paper references
DNA fragmentation in childhood T-cell acute lymphoblastic leukaemia.
S. Kumar (1987)
Well-differentiated B cell lymphomas/leukaemias express IL-2 receptors identified by immunofluorescence and flow cytometry.
P. De Paoli (1987)
Mitogen-stimulated immunoglobulin production by chronic lymphocytic leukaemic lymphocytes.
A. Johnstone (1982)
977) Death of cells by apoptosis following attachment of specifically allergized lymphoqytes in vitro
J Searle
10.1098/rspb.1977.0078
The mechanism of K cell (antibody-dependent) cell mediated cytotoxicity II. Characteristics of the effector cell and morphological changes in the target cell
C. Sanderson (1977)
Control of B cell proliferation: arrest of B cells in late G1 underlies immunosuppression induced by plasma cell tumors.
J. Berman (1987)
T lymphocytes in infectious mononucleosis. I. T cell death in vitro.
D. Moss (1985)
10.1038/284555A0
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
A. Wyllie (1980)
Biophysical and morphological correlates of kinetic change and death in a starved human melanoma cell line.
J. Sheridan (1981)
10.1038/ICB.1977.38
Death of cells by apoptosis following attachment of specifically allergized lymphocytes in vitro.
M. Don (1977)
The role of reactive oxygen metabolites in lymphocyte-mediated cytolysis.
C. Bishop (1987)
10.1172/JCI109567
Poor mixed lymphocyte reaction stimulatory capacity of leukemic B cells of chronic lymphocytic leukemia patients despite the presence of Ia antigens.
J. P. Halper (1979)
10.1084/JEM.161.5.1225
Interleukin 2-induced proliferation of leukemic human B cells
O. Lantz (1985)
T lymphocytes in infectious mononucleosis. II. Response in vitro to interleukin-2 and establishment of T cell lines.
C. Bishop (1985)
Control of B cell proliferation: inhibition of responses to B cell mitogens induced by plasma cell tumors.
J. Berman (1985)
10.1111/j.1600-065X.1979.tb00297.x
Differentiation Capacity and Other Properties of the Leukemic Cells of Chronic Lymphocytic Leukemia *
S. Fu (1979)
10.1093/CARCIN/5.4.453
Controlled death (apoptosis) of normal and putative preneoplastic cells in rat liver following withdrawal of tumor promoters.
W. Bursch (1984)
Differences in target cell DNA fragmentation induced by mouse cytotoxic T lymphocytes and natural killer cells.
R. C. Duke (1986)
Well-differentiated B cell
P. de Paoli (1987)
Apoptosis as the mode of cell death in antibody-dependent lymphocytotoxicity.
N. Stacey (1985)
10.1016/S0140-6736(83)90306-9
ANTIBODIES TO ATLV (HTLV) IN NIGERIAN BLOOD DONORS AND PATIENTS WITH CHRONIC LYMPHATIC LEUKAEMIA OR LYMPHOMA
AlanF. Fleming (1983)
Metabolism of radio-iodinated IgG in patients with abnormal serum IgG levels. II. Hypogamma-globulinaemia.
J. Wells (1971)
10.1038/312641A0
Human interleukin-2 promotes proliferation of activated B cells via surface receptors similar to those of activated T cells
M. Mingari (1984)
Characterization of a human retrovirus from cultured chronic lymphocytic leukemic B - cells . ( Abstract )
C. R.. Moscoso. H. Kiefer (1984)
Normal and certain leukaemic B cells express IL-2 receptors without in vitro activation.
R. Armitage (1986)
Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death. Federation of
U A Pechatnikov (1986)
Origin and function of suppressor macrophages in myeloma.
J. Kennard (1980)
10.1016/0167-5699(86)90152-0
Death and the cell.
E. Duvall (1986)
10.1016/0022-1759(77)90193-4
Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes.
H. Zola (1977)
10.1016/S0140-6736(74)91776-0
T LYMPHOCYTES IN INFECTIOUS MONONUCLEOSIS
B. Seitanidis (1974)
987) Control of'B cell proliferation. Arrest of B cells in late GI underlies immunosuppression induced by plasma cell tumours
J E Berman
The cellular and subcellular characteristics of acute and chronic injury with emphasis on the role of calcium
B. F. Trump (1982)
10.1016/0022-1759(74)90003-9
An improved rosetting assay for detection of human T lymphocytes.
M. E. Kaplan (1974)
983) Antibody to ATLV (HTLV) in Nigerian blood donors and patients with chronic lymphocyte leukemia or lymphoma. (Letter). hncet. ii
N Yamamoto
10.1016/S0140-6736(72)90624-1
Response of lymphocytes in chronic lymphocytic leukaemia to plant mitogens.
J. Smith (1972)
10.1080/09553008814550431
Radiation-induced interphase death of rat thymocytes is internally programmed (apoptosis).
T. Yamada (1988)
10.1098/rspb.1977.0100
The mechanism of T cell mediated cytotoxicity V. Morphological studies by electron microscopy
C. Sanderson (1977)
10.1016/0090-1229(75)90025-2
A comparison of human lymphoid cells in antibody-dependent cellular cytotoxicity (ADCC).
R. Gale (1975)
10.1007/978-1-4684-8326-0_32
DNA fragmentation in targets of CTL: an example of programmed cell death in the immune system.
J. J. Cohen (1985)
Myeloma-induced immunosuppression: a multistep mechanism.
J. Katzmann (1978)
10.1016/0014-5793(86)80115-6
Flow cytometry and biochemical analysis of DNA degradation characteristic of two types of cell death
V. I. Afanas’ev (1986)
10.1182/blood.v69.6.1667.1667
The proliferative response of B cell chronic lymphocytic leukemia to interleukin 2: functional characterization of the interleukin 2 membrane receptors.
I. Touw (1987)
In vitro maturation of B cells in chronic lymphocytic leukemia. I. Synergistic action of phorbol ester and interleukin 2 in the induction of Tac antigen expression and interleukin 2 responsiveness in leukemic B cells.
D. Kabelitz (1985)
Mechanisms of immune lysis. III. Characterization of the nature and kinetics of the cytotoxic T lymphocyte-induced nuclear lesion in the target.
J. H. Russell (1982)
10.1073/PNAS.80.20.6361
Endogenous endonuclease-induced DNA fragmentation: an early event in cell-mediated cytolysis.
R. C. Duke (1983)
10.1038/bjc.1987.186
Rodent fibroblast tumours expressing human myc and ras genes: growth, metastasis and endogenous oncogene expression.
A. Wyllie (1987)
10.1093/JNCI/56.2.375
Effects of Rauscher leukemia virus infection on BALB/c mouse embryos.
M. Umar (1976)
Occurrence of cell death (apoptosis) in preneoplastic liver cells
S.G.M.. Rao. P.M.. Rajalakshmi (1984)
Occurrence of cell death (apoptosis) in preneoplastic and neoplastic liver cells. A sequential study.
A. Columbano (1984)
Intrinsic B cell defect in the immunoglobulin deficiency of chronic lyrnphocytic leukemia . ( Abstract )
R. Pratt.K. (1977)
Intrinsic B cell defect
A. S. Fauci (1977)
Controlled death (apoptosis) of normal and putative preneoplastic cells in rat liver following withdrawal of tumour promoters. Carcinogenesis
R. Schulte-Hermann (1984)
The mechanism of T cell mediated cytotoxicity . V . Morphological studies by electron mic - ology , 3 , 377 - 384 . 1141 - 1148
M. Glauert.A. (1977)
10.1182/BLOOD.V62.4.767.BLOODJOURNAL624767
Immunoglobulin secretory function of B cells from untreated patients with chronic lymphocytic leukemia and hypogammaglobulinemia: role of T cells.
L. Fernandez (1983)
10.1002/PATH.1711420112
Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis
A. Wyllie (1984)
Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance.
J. Searle (1982)
10.1016/S0074-7696(08)62312-8
Cell death: the significance of apoptosis.
A. Wyllie (1980)
10.1111/j.1365-2141.1981.tb07242.x
B Lymphocyte Function in B Cell Chronic Lymphocytic Leukaemia
J. Wolos (1981)
10.1038/bjc.1983.211
The role of natural killer cells in the intravascular death of intravenously injected murine tumour cells.
C. Bishop (1983)
Positive effects of interferon-alpha on B cell-type chronic lymphocytic leukemia proliferative response.
S. Karray (1988)



This paper is referenced by
10.1182/BLOOD.V91.7.2387.2387_2387_2396
Chronic Lymphocytic Leukemic B Cells But Not Normal B Cells Are Rescued From Apoptosis by Contact With Normal Bone Marrow Stromal Cells
L. Lagneaux (1998)
10.1159/000105678
Intracellular Cytokine Expression by T Cells Differs in ZAP-70-Positive and ZAP-70-Negative Chronic Lymphocytic Leukaemia Patients
M. Podhorecka (2007)
10.1046/j.1365-2141.2001.02553.x
Early induction of apoptosis in B‐chronic lymphocytic leukaemia cells by hydroxychloroquine: activation of caspase‐3 and no protection by survival factors
L. Lagneaux (2001)
apoptosis lymphocytic leukemia cells from chlorambucil-and radiation-induced Albumin activates the AKT signaling pathway and protects B-chronic
Gitendra Wickremasinghe Bruckdorfer (2002)
10.1038/sj.cdd.4402211
Crosstalk among Bcl-2 family members in B-CLL: seliciclib acts via the Mcl-1/Noxa axis and gradual exhaustion of Bcl-2 protection
D. Hallaert (2007)
10.21037/TCR.2018.06.21
Casein kinase 1: a new tale of chronic lymphocytic leukemia (CLL) microenvironment
Stefania Fiorcari (2018)
10.1182/blood.v81.1.143.143
Induction of apoptotic cell death in chronic lymphocytic leukemia by 2-chloro-2'-deoxyadenosine and 9-beta-D-arabinosyl-2-fluoroadenine.
L. Robertson (1993)
Pre-clinical development of novel ROR1 chimeric antigen receptor T cells and bispecific T cell engagers
Satyen H. Gohil (2018)
10.3109/10428199509056850
Autocrine and paracrine regulation of neoplastic cell growth in hairy cell leukemia.
M. Schmid (1995)
10.1016/S0027-5107(99)00224-9
Kinetics of excision repair of UV-induced DNA damage, measured using the comet assay.
M. Myllyperkiö (2000)
10.3324/haematol.2011.054924
Physical contact with endothelial cells through β1- and β2- integrins rescues chronic lymphocytic leukemia cells from spontaneous and drug-induced apoptosis and induces a peculiar gene expression profile in leukemic cells
R. Maffei (2012)
10.18632/oncotarget.8672
STAT3 and NF-κB cooperatively control in vitro spontaneous apoptosis and poor chemo-responsiveness in patients with chronic lymphocytic leukemia
F. Liu (2016)
10.1002/CYTO.990200309
Adaptation of the TdT assay for semi-quantitative flow cytometric detection of DNA strand breaks.
T. Bromidge (1995)
Evaluation of LMP-420: A Novel, Nontoxic Drug with Anti-Inflammatory Properties and Therapeutic Potential for CLL
Y. Mowery (2012)
10.1182/BLOOD.V99.8.2969
Involvement of protein kinase C and phosphatidylinositol 3-kinase pathways in the survival of B-cell chronic lymphocytic leukemia cells.
M. Barragán (2002)
10.2307/3578527
Radiation-induced DNA single-strand breaks in freshly isolated human leukocytes.
J. Buatti (1992)
10.1371/journal.pone.0163982
Extracellular Histone Released from Leukemic Cells Increases Their Adhesion to Endothelium and Protects them from Spontaneous and Chemotherapy-Induced Leukemic Cell Death
H. Yoo (2016)
Decrease in serum levels of interleukin-1 β , interleukin-5 and interleukin-9 after vitamin D repletion in patients with chronic lymphocytic leukemia
M. Kubeczko (2016)
10.1038/leu.2009.151
The BH3 mimetic compound, ABT-737, synergizes with a range of cytotoxic chemotherapy agents in chronic lymphocytic leukemia
K. Mason (2009)
10.1111/J.1574-695X.1996.TB00351.X
Lipopolysaccharide induces apoptotic cell death of B memory cells and regulates B cell memory in antigen-nonspecific manner.
T. Yokochi (1996)
10.3109/10428190109099331
Expression and Production of Interleukin 4 in B-Cell Chronic Lymphocytic Leukaemia
T. Mainou-Fowler (2001)
10.1182/blood-2010-05-284505
Soluble CD14 is a novel monocyte-derived survival factor for chronic lymphocytic leukemia cells, which is induced by CLL cells in vitro and present at abnormally high levels in vivo.
M. Seiffert (2010)
10.1182/blood-2012-02-414060
Decreased mitochondrial apoptotic priming underlies stroma-mediated treatment resistance in chronic lymphocytic leukemia.
M. Davids (2012)
10.1046/j.1365-2141.1998.00595.x
Diminished production of interleukin‐6 in chronic lymphocytic leukaemia (B‐CLL) cells from patients at advanced stages of disease
J. Hulkkonen (1998)
10.3109/10428199609067578
Interferon-alpha, Bcl-2 expression and apoptosis in B-cell chronic lymphocytic leukemia.
A. Jewell (1996)
10.1080/10428199909169609
Adhesion to bone marrow stroma inhibits apoptosis of chronic lymphocytic leukemia cells.
L. Lagneaux (1999)
From the Department of Oncology and Pathology Karolinska Institutet, Stockholm, Sweden Targeted monoclonal antibody therapy in chronic lymphocytic leukemia
Claes Karlsson (2010)
10.1182/BLOOD-2005-04-1718
ZAP-70 expression is associated with enhanced ability to respond to migratory and survival signals in B-cell chronic lymphocytic leukemia (B-CLL).
S. Richardson (2006)
10.1182/BLOOD.V100.5.1795.H81702001795_1795_1801
Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1.
I. Pedersen (2002)
Functional Role of Glycogen synthase Kinase-3β on Glucocorticoid-mediated signaling
C. Patiño (2012)
10.1080/10428190290011921
Chemokine Receptors and Stromal Cells in the Homing and Homeostasis of Chronic Lymphocytic Leukemia B Cells
J. Burger (2002)
10.1158/1078-0432.CCR-08-1673
The Novel Nuclear Factor-κB Inhibitor LC-1 Is Equipotent in Poor Prognostic Subsets of Chronic Lymphocytic Leukemia and Shows Strong Synergy with Fludarabine
S. Hewamana (2008)
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