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Different Involvement Of The Mitochondrial, Plastidial And Cytosolic Ascorbate-glutathione Redox Enzymes In Heat Shock Responses.

V. Locato, M. D. de Pinto, L. De Gara
Published 2009 · Medicine, Biology

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Plant survival under heat stress requires the activation of proper defence mechanisms to avoid the impairment of metabolic functions. Heat stress leads to the overproduction of reactive oxygen species (ROS) in the cell. In plants, the ascorbate (ASC)-GSH cycle plays a pivotal role in controlling ROS levels and cellular redox homeostasis. Ascorbate peroxidase (APX) is the enzyme of this cycle mainly involved in ROS detoxification. In this study, the ASC-GSH cycle enzymes were analysed in the cytosol, mitochondria and plastids of tobacco Bright Yellow-2 cultured cells. The cells were also subjected to two different heat shocks (HSs; 35 or 55 degrees C for 10 min) and the cell compartments were isolated in both conditions. The results reported here indicate that moderate HS (35 degrees C) does not affect cell viability, whereas cell exposure to 55 degrees C HS induces programmed cell death (PCD). In relation to ASC-GSH cycle, the three analysed compartments have specific enzymatic profiles that are diversely altered by the HS treatments. The cytosol contains the highest activity of all ASC-GSH cycle enzymes and the data reported here suggest that it acts as a redox buffer for the whole cells. In particular, the cytosolic APX seems to be the most versatile enzyme, being its activity enhanced after moderate HS and reduced during PCD induction, whereas the other APX isoenzymes are only affected in the cells undergoing PCD. The relevance of the changes in the different ASC-GSH cycle isoenzymes in allowing cell survival or promoting PCD is discussed.
This paper references
Regulation and function
S Shigeoka (2002)
10.1104/pp.114.1.275
Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves
A. Jiménez (1997)
10.1093/PCP/PCI135
Acclimation to diverse environmental stresses caused by a suppression of cytosolic ascorbate peroxidase in tobacco BY-2 cells.
Takahiro Ishikawa (2005)
10.1034/J.1399-3054.2003.00223.X
Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria
C. Foyer (2003)
10.1042/BJ20031419
Evidence for the transport of glutathione through ryanodine receptor channel type 1.
G. Bánhegyi (2003)
10.1007/s00438-003-0954-8
Two different heat shock transcription factors regulate immediate early expression of stress genes in Arabidopsis
C. Lohmann (2003)
10.1104/PP.127.1.159
Regulation of the Arabidopsis transcriptome by oxidative stress.
R. Desikan (2001)
10.1104/pp.011021
Common Components, Networks, and Pathways of Cross-Tolerance to Stress. The Central Role of “Redox” and Abscisic Acid-Mediated Controls1
G. Pastori (2002)
10.1104/PP.121.3.921
Peroxisomal NADP-Dependent Isocitrate Dehydrogenase. Characterization and Activity Regulation during Natural Senescence.
Corpas (1999)
10.1104/PP.116.4.1351
Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings
Dat (1998)
10.1101/087969502.34.715
The Role of Ascorbate Peroxidase and Monodehydroascorbate Reductase in H 2 O 2 Scavenging in Plants
K. Asada (1997)
Ascorbate metabolism and plant growth – from germination to cell death
L De Gara (2004)
10.1093/JEXBOT/53.372.1305
Regulation and function of ascorbate peroxidase isoenzymes.
S. Shigeoka (2002)
10.1093/JXB/ERG021
Redox regulation and storage processes during maturation in kernels of Triticum durum.
L. De Gara (2003)
10.1104/PP.100.1.138
Purification of Multiple Forms of Glutathione Reductase from Pea (Pisum sativum L.) Seedlings and Enzyme Levels in Ozone-Fumigated Pea Leaves.
N. Madamanchi (1992)
Parallel changes
JF Dat (1998)
10.1104/PP.123.1.335
Ascorbate biosynthesis in mitochondria is linked to the electron transport chain between complexes III and IV.
C. Bartoli (2000)
10.1111/j.1364-3703.2008.00478.x
Regulation of catalase activity and gene expression during Phytophthora nicotianae development and infection of tobacco.
L. Blackman (2008)
10.1046/J.1365-313X.2003.01715.X
Growth suppression, altered stomatal responses, and augmented induction of heat shock proteins in cytosolic ascorbate peroxidase (Apx1)-deficient Arabidopsis plants.
L. Pnueli (2003)
10.1104/pp.103.035956
Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells1
R. A. Vacca (2004)
10.1104/pp.001362
Heat Stress- and Heat Shock Transcription Factor-Dependent Expression and Activity of Ascorbate Peroxidase in Arabidopsis1
I. I. Panchuk (2002)
10.1093/pcp/pcn013
Increase in ascorbate-glutathione metabolism as local and precocious systemic responses induced by cadmium in durum wheat plants.
A. Paradiso (2008)
10.1105/tpc.105.033589
Redox Homeostasis and Antioxidant Signaling: A Metabolic Interface between Stress Perception and Physiological Responses
C. Foyer (2005)
10.1042/BJ20080287
Plant plasma membrane water channels conduct the signalling molecule H2O2.
M. Dynowski (2008)
10.1038/sj.cdd.4400527
Apoptosis inducing factor (AIF): a phylogenetically old, caspase-independent effector of cell death
H. K. Lorenzo (1999)
10.1016/S0378-1119(00)00043-3
Expression of the chloroplast-localized small heat shock protein by oxidative stress in rice.
B. H. Lee (2000)
Ammonia assimilation
PJ Lea (1980)
10.1016/B978-0-12-675405-6.50010-3
4 – Ammonia Assimilation
B. Miflin (1980)
10.1016/S0981-9428(00)00773-7
Enzymes of the ascorbate biosynthesis and ascorbate-glutathione cycle in cultured cells of tobacco Bright Yellow 2
M. C. Pinto (2000)
10.1093/JXB/ERH194
The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid?
M. Tausz (2004)
10.1016/J.TPLANTS.2004.08.009
Reactive oxygen gene network of plants.
R. Mittler (2004)
10.1007/BF01415704
The redox state of the ascorbate-dehydroascorbate pair as a specific sensor of cell division in tobacco BY-2 cells
M. C. Pinto (2005)
10.1146/ANNUREV.ARPLANT.49.1.249
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control.
G. Noctor (1998)
10.1038/266271A0
Proton pump coupled to cytochrome c oxidase in mitochondria.
Mårten Wikström (1977)
10.1093/OXFORDJOURNALS.PCP.A076232
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Y. Nakano (1981)
10.1104/pp.103.028399
Control of Ascorbate Synthesis by Respiration and Its Implications for Stress Responses1
A. Millar (2003)
10.1105/tpc.13.4.793
DNA Microarray Analysis of Cyanobacterial Gene Expression during Acclimation to High Light
Y. Hihara (2001)
10.1006/BBRC.1997.7946
Purification and molecular properties of ascorbate peroxidase from bovine eye.
N. Wada (1998)
10.1104/pp.106.078683
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death1
R. A. Vacca (2006)
10.1016/S0074-7696(08)62452-3
Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher Plants
T. Nagata (1992)
10.1104/PP.86.2.626
Hydrogen peroxide metabolism in soybean embryonic axes at the onset of germination.
S. Puntarulo (1988)
10.1105/tpc.9.4.627
Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress.
S. Karpiński (1997)
10.1104/pp.105.067686
Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis1
Valeria R Pavet (2005)
10.1016/J.BBABIO.2006.11.004
In the early phase of programmed cell death in Tobacco Bright Yellow 2 cells the mitochondrial adenine nucleotide translocator, adenylate kinase and nucleoside diphosphate kinase are impaired in a reactive oxygen species-dependent manner.
D. Valenti (2007)
10.1104/pp.103.020610
Expression of 1L-Myoinositol-1-Phosphate Synthase in Organelles1
Kimberly Helms Lackey (2003)
10.1126/SCIENCE.284.5414.654
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.
S. Karpiński (1999)
10.1074/jbc.M801998200
Catabolism of Glutathione Conjugates in Arabidopsis thaliana
Melissa Brazier-Hicks (2008)
Reactive oxygen species production, impairment of glucose oxidation and cytosolic ascorbate peroxidase are early events in heat-shock induced programmed cell death in tobacco BY-2 cells
RA Vacca (2004)
10.1111/j.1365-3040.2008.01867.x
Production of reactive species and modulation of antioxidant network in response to heat shock: a critical balance for cell fate.
V. Locato (2008)
Heat stress-induced H 2 O 2 is required for effective expression of heat shock ge
R. Volkov (2006)
10.1146/ANNUREV.ARPLANT.50.1.601
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
K. Asada (1999)
10.1046/J.1365-313X.1995.08020167.X
Simultaneous targeting of pea glutathione reductase and of a bacterial fusion protein to chloroplasts and mitochondria in transgenic tobacco.
G. Creissen (1995)
10.1104/PP.118.4.1327
Role of the ascorbate-glutathione cycle of mitochondria and peroxisomes in the senescence of pea leaves
Jiménez (1998)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
peroxidase from bovine eye
M Wikström (1977)
10.1104/PP.121.2.429
Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment.
H. Chung (1999)
10.1046/J.1365-313X.1998.00056.X
Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells.
N. Banzet (1998)
10.1104/PP.71.4.742
Alcohol dehydrogenase inactivator from rice seedlings : properties and intracellular location.
S. Shimomura (1983)
10.1016/0005-2728(79)90078-1
H2O2 destruction by ascorbate-dependent systems from chloroplasts.
D. Groden (1979)



This paper is referenced by
Hydrogen peroxide is involved in the regulation of rice (Oryza sativa L.) tolerance to salt stress
Xiaomin WangChen (2013)
10.1111/j.1438-8677.2011.00543.x
Galactone-γ-lactone-dependent ascorbate biosynthesis alters wheat kernel maturation.
A. Paradiso (2012)
10.1111/j.1365-3040.2011.02387.x
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
10.1007/s00709-009-0078-z
Effect of heat stress on actin cytoskeleton and endoplasmic reticulum of tobacco BY-2 cultured cells and its inhibition by Co2+
M. Malerba (2009)
10.1016/J.ENVEXPBOT.2018.03.010
Differential Pb tolerance in metallicolous and non-metallicolous Zygophyllum fabago populations involves the strengthening of the antioxidative pathways
M. A. Ferrer (2018)
10.1007/s11103-016-0469-4
Integrated bioinformatics to decipher the ascorbic acid metabolic network in tomato
V. Ruggieri (2016)
10.3389/fenvs.2015.00025
Redox homeostasis via gene families of ascorbate-glutathione pathway
Prachi Pandey (2015)
10.1201/9781439854082-4
Reactive Oxygen Species and Ascorbate-Glutathione Interplay in Signaling and Stress Responses
Vittoria Locato (2010)
10.1111/j.1365-313X.2010.04190.x
H2O2 in plant peroxisomes: an in vivo analysis uncovers a Ca(2+)-dependent scavenging system.
A. Costa (2010)
10.31428/10317/6809
Expression patterns of antioxidant network in pioneer plants from mine tailings polluted with high levels of heavy metals
Antonio López Orenes (2018)
10.1007/s00425-009-1093-1
Comparative proteomic analysis of longan (Dimocarpus longan Lour.) seed abortion
H. Liu (2009)
10.1002/9781119468677.ch14
Oxidative Stress and Antioxidant Defense in Plants Under High Temperature
Pooja (2019)
10.1093/aob/mcv076
Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2 treatment.
A. Ortiz-Espín (2015)
10.1201/9781351104609-7
Oxidative Stress and Antioxidative Defense System in Plants Growing under Abiotic Stresses
P. Sharma (2019)
10.1007/978-3-319-21033-9_2
Mitochondrion and Chloroplast Regulation of Plant Programmed Cell Death
Theresa J. Reape (2015)
10.1007/s00344-010-9189-1
The Distribution and Cooperation of Antioxidant (Iso)enzymes and Antioxidants in Different Subcellular Compartments in Maize Leaves during Water Stress
Mingpu Tan (2010)
ENVIRONMENT-DEPENDENT CAUSES AND CONSEQUENCES OF MUTATION IN SACCHAROMYCES CEREVISIAE
C. Kozela (2012)
Antioxidant Defense System in Wheat (Triticum aestivum L.) Seedlings under Heat Stress and Revival Conditions
Mahla (2011)
10.1007/978-3-319-95315-1_5
Ascorbate Peroxidases: Scavengers or Sensors of Hydrogen Peroxide Signaling?
Andréia Caverzan (2019)
10.1007/978-981-13-9349-5_17
Arbuscular Mycorrhizal Fungi in Alleviation of Cold Stress in Plants
Thokchom Sarda Devi (2019)
10.1007/s11738-012-1132-6
Hydrogen peroxide is involved in the regulation of rice (Oryza sativa L.) tolerance to salt stress
X. Wang (2012)
10.1007/s00709-014-0636-x
Metal/metalloid stress tolerance in plants: role of ascorbate, its redox couple, and associated enzymes
N. Anjum (2014)
10.1186/s12864-015-2225-6
De novo assembly and functional annotation of Myrciaria dubia fruit transcriptome reveals multiple metabolic pathways for L-ascorbic acid biosynthesis
Juan C. Castro (2015)
10.1002/9783527694570.CH16
Cross‐Stress Tolerance in Plants: Molecular Mechanisms and Possible Involvement of Reactive Oxygen Species and Methylglyoxal Detoxification Systems
M. Anwar Hossain (2016)
10.1007/978-3-319-28899-4_6
Interplay Among Glutathione, Salicylic Acid, and Ethylene to Combat Environmental Stress
Sharmila Chattopadhyay (2016)
10.1111/ppl.12220
Changes in antioxidants are critical in determining cell responses to short- and long-term heat stress.
A. Sgobba (2015)
Antioxidative System as Influenced by High Temperature stress in Brassica juncea ( L ) Czern & Coss
B. Rani (2016)
10.3389/fpls.2014.00207
Enhancing crop resilience to combined abiotic and biotic stress through the dissection of physiological and molecular crosstalk
C. Kissoudis (2014)
10.1007/s10535-012-0243-7
Involvement of G6PDH in heat stress tolerance in the calli from Przewalskia tangutica and Nicotiana tabacum
Haifeng Gong (2012)
10.1016/B978-0-12-816451-8.00031-9
Heat Shock Proteins (Hsps) Mediated Signalling Pathways During Abiotic Stress Conditions
Kummari Divya (2019)
10.1016/j.redox.2017.01.018
Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture
A. Calderón (2017)
10.3389/fpls.2013.00152
Strategies to increase vitamin C in plants: from plant defense perspective to food biofortification
V. Locato (2013)
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