← Back to Search
Analysis Of In Situ Manganese(II) Oxidation In The Columbia River And Offshore Plume: Linking Aurantimonas And The Associated Microbial Community To An Active Biogeochemical Cycle.
C. Anderson, R. E. Davis, N. Bandolin, A. M. Baptista, B. Tebo
Published 2011 · Biology, Medicine
Download PDFAnalyze on Scholarcy
The Columbia River is a major source of dissolved nutrients and trace metals for the west coast of North America. A large proportion of these nutrients are sourced from the Columbia River Estuary, where coastal and terrestrial waters mix and resuspend particulate matter within the water column. As estuarine water is discharged off the coast, it transports the particulate matter, dissolved nutrients and microorganisms forming nutrient-rich and metabolically dynamic plumes. In this study, bacterial manganese oxidation within the plume and estuary was investigated during spring and neap tides. The microbial community proteome was fractionated and assayed for Mn oxidation activity. Proteins from the outer membrane and the loosely bound outer membrane fractions were separated using size exclusion chromatography and Mn(II)-oxidizing eluates were analysed with tandem mass spectrometry to identify potential Mn oxidase protein targets. Multi-copper oxidase (MCO) and haem-peroxidase enzymes were identified in active fractions. T-RFLP profiles and cluster analysis indicates that organisms and bacterial communities capable of oxidizing Mn(II) can be sourced from the Columbia River estuary and nearshore coastal ocean. These organisms are producing up to 10 fM MnO₂ cell⁻¹ day⁻¹. Evidence for the presence of Mn(II)-oxidizing bacterial isolates from the genera Aurantimonas, Rhodobacter, Bacillus and Shewanella was found in T-RFLP profiles. Specific Q-PCR probes were designed to target potential homologues of the Aurantimonas manganese oxidizing peroxidase (Mop). By comparing total Mop homologues, Aurantimonas SSU rRNA and total bacterial SSU rRNA gene copies, it appears that Aurantimonas can only account for ~1.7% of the peroxidase genes quantified. Under the broad assumption that at least some of the peroxidase homologues quantified are involved in manganese oxidation, it is possible that other organisms oxidize manganese via peroxidases.
This paper references
Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067
E. Larsen (1999)
A particle conveyor belt process in the Columbia River estuary: Evidence from chlorophylla and particulate organic carbon
L. Small (2004)
Localization of cytochromes to the outer membrane of anaerobically grown Shewanella putrefaciens MR-1.
C. Myers (1992)
Dissolved manganese in the columbia river estuary: production in the water column
G. Klinkhammer (2001)
Manganese(IV) Oxide Production by Acremonium sp. Strain KR21-2 and Extracellular Mn(II) Oxidase Activity
N. Miyata (2006)
Behavior of terrestrial dissolved organic matter at the continent-ocean boundary from high-resolution distributions
G. Klinkhammer (2000)
XPS study of dissolution of birnessite by humate with constraints on reaction mechanism
D. Banerjee (2001)
Direct Identification of a Bacterial Manganese(II) Oxidase, the Multicopper Oxidase MnxG, from Spores of Several Different Marine Bacillus Species
G. Dick (2007)
Importance of vertical mixing for additional sources of nitrate and iron to surface waters of the Columbia River plume: Implications for biology
M. Lohan (2004)
Factors influencing the chemistry of the near‐field Columbia River plume: Nitrate, silicic acid, dissolved Fe, and dissolved Mn
K. W. Bruland (2008)
Novel enzymatic oxidation of Mn2+ to Mn3+ catalyzed by a fungal laccase
C. Höfer (1999)
Extreme spatial and temporal variability of hydrothermal microbial mat communities along the Mariana Island Arc and southern Mariana back‐arc system
R. Davis (2008)
Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.
A. Keller (2002)
Laccase-Catalyzed Oxidation of Mn2+ in the Presence of Natural Mn3+ Chelators as a Novel Source of Extracellular H2O2 Production and Its Impact on Manganese Peroxidase
D. Schlosser (2002)
Mechanism of the oxidation of divalent iron and manganese by iron bacteria developing in a neutral acidic medium
Dubinina Ga (1978)
ARB: a software environment for sequence data.
W. Ludwig (2004)
Bacterial activity and genetic richness along an estuarine gradient (Rhone River plume, France)
M. Troussellier (2002)
Organic carbon and nitrogen in the northern California current system: comparison of offshore, river plume, and coastally upwelled waters
J. K. Hill (2002)
Particle-attached bacteria and heterotrophic plankton associated with the Columbia River estuarine turbidity maxima
B. Crump (1996)
Particle Trapping in Estuarine Tidal Flows
D. Jay (1994)
Biotransformations of Manganese
B. Tebo (2007)
Rapid, oxygen-dependent microbial Mn(II) oxidation kinetics at sub-micromolar oxygen concentrations in the Black Sea suboxic zone
B. Clement (2009)
BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT
T. A. Hall (1999)
Manganese(II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators.
H. Wariishi (1992)
Suspended-sediment response to semidiurnal and fortnightly tidal variations in a mesotidal estuary: Columbia River, U.S.A.
G. Gelfenbaum (1983)
Chapter 1 Broad-Scale Distributional Patterns of Hydrographic Variables on the Washington/Oregon Shelf
M. Landry (1989)
CHEMICAL AND BIOLOGICAL REDUCTION OF MN (III)-PYROPHOSPHATE COMPLEXES : POTENTIAL IMPORTANCE OF DISSOLVED MN (III) AS AN ENVIRONMENTAL OXIDANT
J. Kostka (1995)
Coastal oceanography of Washington and Oregon
M. Landry (1989)
Kinetics of Redox Reactions on Manganese Oxides and Its Impact on Environmental Quality
P. Huang (2015)
A bi-directional river plume: The Columbia in summer
B. Hickey (2005)
Iron and manganese in anaerobic respiration: environmental significance, physiology, and regulation.
K. H. Nealson (1994)
Microbial reduction of manganese and iron: new approaches to carbon cycling.
K. H. Nealson (1992)
Occurrence and Mechanisms of Microbial Oxidation of Manganese
K. Nealson (1988)
Inter-relationships of MnO2 precipitation, siderophore–Mn(III) complex formation, siderophore degradation, and iron limitation in Mn(II)-oxidizing bacterial cultures
Dorothy L. Parker (2007)
Biogenic manganese oxides: Properties and mechanisms of formation
B. Tebo (2004)
Diverse Mn(II)-Oxidizing Bacteria Isolated from Submarine Basalts at Loihi Seamount
A. Templeton (2005)
Culturable Rhodobacter and Shewanella species are abundant in estuarine turbidity maxima of the Columbia River.
S. Bräuer (2011)
Phylogenetic Analysis of Particle-Attached and Free-Living Bacterial Communities in the Columbia River, Its Estuary, and the Adjacent Coastal Ocean
B. Crump (1999)
A multicopper oxidase is essential for manganese oxidation and laccase-like activity in Pedomicrobium sp. ACM 3067.
J. P. Ridge (2007)
A statistical model for identifying proteins by tandem mass spectrometry.
A. Nesvizhskii (2003)
Biogeochemical characterization of suspended particulate matter in the Columbia River estuary
F. Prahl (1997)
In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation
H. A. Johnson (2007)
Photoreduction of manganese oxides in seawater
W. Sunda (1994)
Unusual ribulose-1,5-bisphosphate carboxylase/oxygenase genes from a marine manganese-oxidizing bacterium.
R. Caspi (1996)
Bacterially mediated mineral formation: Insights into manganese(II) oxidation from molecular genetic and biochemical studies
B. Tebo (1997)
Manganese ii oxidation in the suboxic zone of the black sea
B. Tebo (1991)
Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays
N. Fierer (2005)
Localization and Solubilization of the Iron(III) Reductase of Geobacter sulfurreducens
S. Gaspard (1998)
Reaction Rates and Products of Manganese Oxidation at the Sediment-Water Interface
B. Wehrli (1995)
Enzymatic microbial Mn(II) oxidation and Mn biooxide production in the Guaymas Basin deep-sea hydrothermal plume
G. Dick (2009)
Soluble Mn(III) in Suboxic Zones
Robert E. Trouwborst (2006)
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB
Elmar Pruesse (2007)
Dominance of particle-attached bacteria in the Columbia River estuary, USA
B. Crump (1998)
cumA Multicopper Oxidase Genes from Diverse Mn(II)-Oxidizing and Non-Mn(II)-OxidizingPseudomonas Strains
C. A. Francis (2001)
The Role of Mn(II)-Peroxidase Activity of Mycobacterial Catalase-Peroxidase in Activation of the Antibiotic Isoniazid*
R. Magliozzo (1997)
Aurantimonas manganoxydans, sp. nov. and Aurantimonas litoralis, sp. nov.: Mn(II) Oxidizing Representatives of a Globally Distributed Clade of alpha-Proteobacteria from the Order Rhizobiales
C. Anderson (2009)
The anaerobic degradation of organic matter in Danish coastal sediments: iron reduction, manganese reduction, and sulfate reduction.
D. Canfield (1993)
Dissolved manganese and silicic acid in the Columbia River plume: A major source to the California current and coastal waters off Washington and Oregon
A. Aguilar-Islas (2006)
Geomicrobiology of manganese(II) oxidation.
B. Tebo (2005)
Coastal Sedimentary Environments
R. Davis (2011)
Mn(II) Oxidation Is Catalyzed by Heme Peroxidases in “Aurantimonas manganoxydans” Strain SI85-9A1 and Erythrobacter sp. Strain SD-21
C. Anderson (2009)
Identification of a Two-Component Regulatory Pathway Essential for Mn(II) Oxidation in Pseudomonas putida GB-1
K. Geszvain (2009)
THE CIRCULATION AND SELECTED PROPERTIES OF THE COLUMBIA RIVER EFFLUENT AT SEA.
C. Barnes (1970)
Coupled Photochemical and Enzymatic Mn(II) Oxidation Pathways of a Planktonic Roseobacter-Like Bacterium
C. M. Hansel (2006)
Dissimilatory Fe(III) and Mn(IV) reduction by Shewanella putrefaciens requires ferE, a homolog of the pulE (gspE) type II protein secretion gene.
T. DiChristina (2002)
In the sea
D. Dixon (2001)
Reduction and dissolution of manganese(III) and manganese(IV) oxides by organics: 2. Survey of the reactivity of organics.
A. Stone (1984)
Reduction and dissolution of manganese(III) and manganese(IV) oxides by organics. 1. Reaction with hydroquinone.
A. Stone (1984)
This paper is referenced by
Investigation of Fe(II) and Mn(II) involved anoxic denitrification in agricultural soils with high manganese and iron contents
Baokun Xu (2020)
Production of Manganese Oxide Nanoparticles by Shewanella Species
M. Wright (2016)
CotA, a Multicopper Oxidase from Bacillus pumilus WH4, Exhibits Manganese-Oxidase Activity
Jianmei Su (2013)
The use of natural Mn oxide-containing wastes as a contaminated land remediation strategy and their effects on soil microbial functioning
C. M. McCann (2012)
Biological Low-pH Mn(II) Oxidation in a Manganese Deposit Influenced by Metal-Rich Groundwater
Tsing Bohu (2016)
Comparative proteomics of Mn(II)-oxidizing and non-oxidizing Roseobacter clade bacteria reveal an operative manganese transport system but minimal Mn(II)-induced expression of manganese oxidation and antioxidant enzymes.
D. R. Learman (2014)
Extracellular haem peroxidases mediate Mn(II) oxidation in a marine Roseobacter bacterium via superoxide production.
Peter F Andeer (2015)
Heterologous Expression and Characterization of the Manganese-Oxidizing Protein from Erythrobacter sp . Strain SD 21
Katherine Nakama (2014)
Fine-scale Microbial Communities Associated with Manganese Nodules in Deep-sea Sediment of the Korea Deep Ocean Study Area in the Northeast Equatorial Pacific
H. Cho (2018)
Heterologous Expression and Characterization of the Manganese-Oxidizing Protein from Erythrobacter sp. Strain SD21
Katherine Nakama (2014)
Infrastructure for collaborative science and societal applications in the Columbia River estuary
A. Baptista (2015)
Biotic and Abiotic Mechanisms of Manganese (II) Oxidation in Lake Erie
Casey M Godwin (2020)
Removal of Manganese(II) from Acid Mine Wastewater: A Review of the Challenges and Opportunities with Special Emphasis on Mn-Oxidizing Bacteria and Microalgae
Yongchao Li (2019)
A Novel Manganese Oxidising Bacterium: Characterisation and Genomic Evaluation
Joanna Smith (2014)
Degradation of emerging contaminants by Fe- and Mn- based oxidation methods in aqueous solution
Xiao Xiao (2012)
MopA, the Mn Oxidizing Protein From Erythrobacter sp. SD-21, Requires Heme and NAD+ for Mn(II) Oxidation
M. Medina (2018)
Biogenic precipitation of manganese oxides and enrichment of heavy metals at acidic soil pH
S. Mayanna (2014)
Metagenomic analysis reveals microbial diversity and function in the rhizosphere soil of a constructed wetland
Y. Bai (2014)
Modern precipitation of hydrogenetic ferromanganese minerals during on-site 15-year exposure tests
A. Usui (2020)
The Role of Bacterial Spores in Metal Cycling and Their Potential Application in Metal Contaminant Bioremediation.
Cristina N Butterfield (2016)
Analysis of manganese oxidase and its encoding gene in Lysinibacillus strain MK-1
Wenwei Tang (2019)
Multiple organic substrates support Mn(II) removal with enrichment of Mn(II)-oxidizing bacteria.
Ahmad Shoiful (2020)