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Stimulation Of The Cytosolic Receptor For Peptidoglycan, Nod1, By Infection With Chlamydia Trachomatis Or Chlamydia Muridarum

Lynn Welter-Stahl, D. Ojcius, J. Viala, S. Girardin, W. Liu, C. Delarbre, D. Philpott, K. Kelly, T. Darville
Published 2006 · Biology, Medicine

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Infection of epithelial cells by the intracellular pathogen, Chlamydia trachomatis, leads to activation of NF‐κB and secretion of pro‐inflammatory cytokines. We find that overexpression of a dominant‐negative Nod1 or depletion of Nod1 by RNA interference inhibits partially the activation of NF‐κB during chlamydial infection in vitro, suggesting that Nod1 can detect the presence of Chlamydia. In parallel, there is a larger increase in the expression of pro‐inflammatory genes following Chlamydia infection when primary fibroblasts are isolated from wild‐type mice than from Nod1‐deficient mice. The Chlamydia genome encodes all the putative enzymes required for proteoglycan synthesis, but proteoglycan from Chlamydia has never been detected biochemically. Since Nod1 is a ubiquitous cytosolic receptor for peptidoglycan from Gram‐negative bacteria, our results suggest that C. trachomatis and C. muridarum do in fact produce at least the rudimentary proteoglycan motif recognized by Nod1. Nonetheless, Nod1 deficiency has no effect on the efficiency of infection, the intensity of cytokine secretion, or pathology in vaginally infected mice, compared with wild‐type controls. Similarly, Rip2, a downstream mediator of Nod1, Toll‐like receptor (TLR)‐2, and TLR4, increases only slightly the intensity of chlamydial infection in vivo and has a very mild effect on the immune response and pathology. Thus, Chlamydia may not produce sufficient peptidoglycan to stimulate Nod1‐dependent pathways efficiently in infected animals, or other receptors of the innate immune system may compensate for the absence of Nod1 during Chlamydia infection in vivo.
This paper references
Tax induces nuclear translocation of NFkappa B through dissociation of cytoplasmic complexes Recognition of Chlamydia by the cytosolic receptor
G Munoz (1057)
Cell Biology
J. Mitchison (1964)
A new animal model for the study of Chlamydia trachomatis genital infections: infection of mice with the agent of mouse pneumonitis.
A. Barron (1981)
A new animal model for the study
E. B. Moses (1981)
Humoral immune response to chlamydial genital infection of mice with the agent of mouse pneumonitis
K. Ramsey (1989)
Interaction of chlamydiae and host cells in vitro.
J. Moulder (1991)
Effect of gamma interferon on resolution of murine chlamydial genital infection
R. Rank (1992)
The characterization of the promoter of the gene encoding the p50 subunit of NF‐kappa B indicates that it participates in its own regulation.
R. Ten (1992)
Resolution of murine chlamydial genital infection by the adoptive transfer of a biovar-specific, Th1 lymphocyte clone.
J. Igietseme (1993)
Resolution of murine
T. J. Kincy (1993)
Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3
E. Muñoz (1994)
Interactions between macrophages and chlamydiae.
D. La Verda (1994)
Tax induces nuclear translocation of NFkappa B through dissociation of cytoplasmic complexes
G. Munoz (1994)
Global data on blindness.
B. Thylefors (1995)
Local Th1-like responses are induced by intravaginal infection of mice with the mouse pneumonitis biovar of Chlamydia trachomatis
T. Cain (1995)
Local Th1-like responses
T. K. 1631–1640. Cain (1995)
Role of neutrophils in controlling early stages of a Chlamydia trachomatis infection
N. Barteneva (1996)
Immunity to Chlamydia trachomatis is mediated by T helper 1 cells through IFN-gamma-dependent and -independent pathways.
L. Perry (1997)
Mouse strain-dependent variation in the course and outcome of chlamydial genital tract infection is associated with differences in host response
T. Darville (1997)
Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.
S. Rasmussen (1997)
Antibiotics, peptidoglycan synthesis and genomics: the chlamydial anomaly revisited.
I. Chopra (1998)
Internalization of Chlamydia by dendritic cells and stimulation of Chlamydia-specific T cells.
D. Ojcius (1998)
Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis.
R. Stephens (1998)
Role of NK Cells in Early Host Response to Chlamydial Genital Infection
Chien-Te Kent Tseng (1998)
bal epidemiology of sexually transmitted diseases
A. C. Gerbase (1998)
Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB.
N. Inohara (1999)
Nod1, an Apaf-1-like Activator of Caspase-9 and Nuclear Factor-κB*
N. Inohara (1999)
Chlamydia: Intracellular Biology, Pathogenesis, And Immunity
R. Stephens (1999)
Human CARD4 Protein Is a Novel CED-4/Apaf-1 Cell Death Family Member That Activates NF-κB*
J. Bertin (1999)
Models of Immunity
R. Rank (1999)
Lack of Cell Wall Peptidoglycan versus Penicillin Sensitivity: New Insights into the Chlamydial Anomaly
Jean-Marie Ghuysen (1999)
Mechanisms of Chlamydia-Induced Disease
M. Ward (1999)
Comparative genomes of Chlamydia pneumoniae and C. trachomatis
S. Kalman (1999)
Cell biology. In Chlamydia: Intracellular
T. Hackstadt (1999)
Nod 1 , an Apaf - 1 - like activator of caspase - 9 and nuclear factor - kappaB
N. Inohara (1999)
Comparative genomes
Hyman (1999)
Lipopolysaccharide Activates Distinct Signaling Pathways in Intestinal Epithelial Cell Lines Expressing Toll-Like Receptors1
E. Cario (2000)
Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39.
T. Read (2000)
Nuclear factor-kappa B activation and innate immune response in microbial pathogen infection.
M. Naumann (2000)
Intracellular survival by Chlamydia
P. Wyrick (2000)
Invasive Shigella flexneri Activates NF-κB Through a Lipopolysaccharide-Dependent Innate Intracellular Response and Leads to IL-8 Expression in Epithelial Cells1
D. Philpott (2000)
Lipopolysaccha - ride activates distinct signaling pathways in intestinal epithelial cell lines expressing Toll - like receptors
E. Cario (2000)
Early Local Cytokine Profiles in Strains of Mice with Different Outcomes from Chlamydial Genital Tract Infection
T. Darville (2001)
CARD4/Nod1 mediates NF-kappaB and JNK activation by invasive Shigella flexneri.
S. Girardin (2001)
CARD4/Nod1 mediates NF‐κB and JNK activation by invasive Shigella flexneri
S. Girardin (2001)
Proapoptotic BAX and BAK: A Requisite Gateway to Mitochondrial Dysfunction and Death
M. Wei (2001)
Radical changes to chlamydial taxonomy are not necessary just yet.
J. Schachter (2001)
Intracellular vs extracellular recognition of pathogens--common concepts in mammals and flies.
S. Girardin (2002)
Innate immune recognition.
C. Janeway (2002)
RICK/Rip2/CARDIAK mediates signalling for receptors of the innate and adaptive immune systems
K. Kobayashi (2002)
Immunity to Murine Chlamydial Genital Infection
R. Morrison (2002)
Decoding the Patterns of Self and Nonself by the Innate Immune System
R. Medzhitov (2002)
Involvement of receptor-interacting protein 2 in innate and adaptive immune responses
A. Chin (2002)
Protein signatures distinctive of chlamydial species: horizontal transfers of cell wall biosynthesis genes glmU from archaea to chlamydiae and murA between chlamydiae and Streptomyces.
E. Griffiths (2002)
Immunity to murine
R. P. Morrison (2002)
Involvement of receptor-interacting © 2006 The Authors Journal compilation © protein 2 in innate and adaptive immune responses
A I Chin (2002)
Involvement of receptor-interacting
G. H. Cheng (2002)
Innate immune
C. A. 355–383. Janeway (2002)
Chlamydial genital infection.
T. Moss (2003)
Host Recognition of Bacterial Muramyl Dipeptide Mediated through NOD2
N. Inohara (2003)
Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae.
T. Read (2003)
Toll-Like Receptor-2, but Not Toll-Like Receptor-4, Is Essential for Development of Oviduct Pathology in Chlamydial Genital Tract Infection 1
T. Darville (2003)
Inhibition of chlamydial infectious activity due to P2X7R-dependent phospholipase D activation.
R. Coutinho-Silva (2003)
Functional and Biochemical Analysis of Chlamydia trachomatis MurC, an Enzyme Displaying UDP-N-Acetylmuramate:Amino Acid Ligase Activity
L. Hesse (2003)
In vitro and in vivo functional activity of Chlamydia MurA, a UDP-N-acetylglucosamine enolpyruvyl transferase involved in peptidoglycan synthesis and fosfomycin resistance.
A. McCoy (2003)
Nod2 Is a General Sensor of Peptidoglycan through Muramyl Dipeptide (MDP) Detection*
S. Girardin (2003)
Global Stage-Specific Gene Regulation during the Developmental Cycle of Chlamydia trachomatis
T. Nicholson (2003)
Nod1 Detects a Unique Muropeptide from Gram-Negative Bacterial Peptidoglycan
S. Girardin (2003)
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis
R. Belland (2003)
An essential role for NOD1 in host recognition of bacterial peptidoglycan containing diaminopimelic acid
Mathias Chamaillard (2003)
Role of Proapoptotic BAX in Propagation of Chlamydia muridarum (the Mouse Pneumonitis Strain of Chlamydia trachomatis) and the Host Inflammatory Response*
J. Perfettini (2003)
Nods, Nalps and Naip: intracellular regulators of bacterial‐induced inflammation
M. Chamaillard (2003)
Toll-like receptor 4-mediated signaling by epithelial surfaces: necessity or threat?
F. Bäckhed (2003)
Host recognition of bacterial muramyl dipeptide mediated through NOD 2 . Implications for Crohn ’ s disease
N. Inohara (2003)
Inhibition of chlamydial infectious activity due to P2X7 receptor-dependent phospholipase
R. Coutinho-Silva (2003)
An essential role for NOD1
L Saab (2003)
Toll-like receptor- 2, but not Toll-like receptor-4, is essential for development of oviduct pathology in chlamydial genital tract infection
M Stahl (2003)
Inhibition of chlamydial infectious activity due to P 2 X 7 receptor - dependent phospholipase D activation
R. Coutinho-Silva (2003)
Functional and bio
D. Lecreulx (2003)
Inhibition of chlamydial infectious activity due to P2X 7 receptor-dependent phospholipase D activation
R Coutinho-Silva (2003)
Toll-like receptor 4-medi
F. Bäckhed (2003)
Host recognition of bacterial
J Crespo (2003)
Inhibition of chlamydial infectious activity due to P 2 X 7 receptordependent phospholipase D activation
R. Coutinho-Silva (2003)
Genomic transcriptional profil
J Sharma (2003)
Toll-like receptor2, but not Toll-like receptor-4, is essential for development of oviduct pathology in chlamydial genital tract infection
T. Darville (2003)
Intracellular Bacterial Infection-Induced IFN-γ Is Critically but Not Solely Dependent on Toll-Like Receptor 4-Myeloid Differentiation Factor 88-IFN-αβ-STAT1 Signaling1
A. Rothfuchs (2004)
Toll-like Receptor 9–Dependent and –Independent Dendritic Cell Activation by Chromatin–Immunoglobulin G Complexes
Melissa W. Boulé (2004)
Intracellular bacterial infection-induced IFN-gamma is critically but not solely dependent on Toll-like receptor 4-myeloid differentiation factor 88-IFN-alpha beta-STAT1 signaling.
A. Rothfuchs (2004)
mRNA Is an Endogenous Ligand for Toll-like Receptor 3*
K. Karikó (2004)
The Infecting Dose of Chlamydia muridarum Modulates the Innate Immune Response and Ascending Infection
Heather K Maxion (2004)
Use of murine embryonic fibroblasts to define Toll-like receptor activation and specificity
E. Kurt-Jones (2004)
IMMUNOLOGY: After the Toll Rush
L. O’Neill (2004)
Nat Rev Microbiol
R Belland (2004)
After the Toll rush
O Neill (2004)
Toll - like receptor 9dependent andindependent dendritic cell activation by chromatinimmunoglobulin G complexes
M. W. Boule (2004)
After the Toll rush
L. A. O’Neill (2004)
mRNA is an endogenous ligand
D. Weissman (2004)
Nod1-Mediated Endothelial Cell Activation by Chlamydophila pneumoniae
B. Opitz (2005)
Chlamydia trachomatis Induces Expression of IFN-γ-Inducible Protein 10 and IFN-β Independent of TLR2 and TLR4, but Largely Dependent on MyD881
U. Nagarajan (2005)
Pattern Recognition Molecules Activated by Chlamydia muridarum Infection of Cloned Murine Oviduct Epithelial Cell Lines1
W. Derbigny (2005)
Chlamydia trachomatis induces expression of IFN-gamma-inducible protein 10 and IFN-beta independent of TLR2 and TLR4, but largely dependent on MyD88.
U. Nagarajan (2005)
NOD-LRR proteins: role in host-microbial interactions and inflammatory disease.
Inohara (2005)
Toll-like receptors.
H. S. Warren (2005)
MyD88 Is Pivotal for the Early Inflammatory Response and Subsequent Bacterial Clearance and Survival in a Mouse Model of Chlamydia pneumoniae Pneumonia*
Y. Naiki (2005)
Tissue-specific mRNA expression profiles of human toll-like receptors and related genes.
M. Nishimura (2005)
[Toll-like receptor].
K. Takeda (2005)
Polymorphonuclear Neutrophils Improve Replication of Chlamydia pneumoniae In Vivo upon MyD88-Dependent Attraction1
N. Rodríguez (2005)
tern recognition molecules activated by Chlamydia muri - darum infection of cloned murine oviduct epithelial cell lines
W. A. Derbigny (2005)
The Authors Journal compilation
1056 L. Welter-Stahl (2006)
Global epidemiology of sexually transmitted diseases.
C. D. Da Ros (2008)
Proc Natl Acad Sci

This paper is referenced by
Immunopathogenesis of genital Chlamydia infection: insights from mouse models.
Jacob Dockterman (2021)
Persistence Alters the Interaction between Chlamydia trachomatis and Its Host Cell
Mary Brockett (2021)
The diverse roles of RIP kinases in host-pathogen interactions
Vik Ven Eng (2020)
NOD1/NOD2 and RIP2 Regulate Endoplasmic Reticulum Stress-Induced Inflammation during Chlamydia Infection
O. Pham (2020)
Microorganisms in the Placenta: Links to Early-Life Inflammation and Neurodevelopment in Children
M. S. Tomlinson (2019)
The Pathogenic Neisseria Use a Streamlined Set of Peptidoglycan Degradation Proteins for Peptidoglycan Remodeling, Recycling, and Toxic Fragment Release
Ryan E Schaub (2019)
TLR3 Deficiency Leads to Altered Immune Responses to Chlamydia trachomatis Infection in Human Oviduct Epithelial Cells
Jerry Z Xu (2019)
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection
R. Kumar (2019)
Toll-Like Receptor 3 Deficiency Leads to Altered Immune Responses to Chlamydia trachomatis Infection in Human Oviduct Epithelial Cells
Jerry Z Xu (2019)
Immunobiology of monocytes and macrophages during Chlamydia trachomatis infection.
Mads Lausen (2019)
Expression of Nod-like Receptors and Clinical Correlations in Patients With Dry Eye Disease.
Yung-Hui Kim (2019)
Cynthia Tram (2018)
Biology of Chlamydia
G. Häcker (2018)
Caveolin-mediated endocytosis of the Chlamydia M278 outer membrane peptide encapsulated in poly(lactic acid)-Poly(ethylene glycol) nanoparticles by mouse primary dendritic cells enhances specific immune effectors mediated by MHC class II and CD4+ T cells.
S. Dixit (2018)
Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice
S. Carrasco (2018)
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection
R. Kumar (2018)
NOD1 and NOD2 in inflammation, immunity and disease.
T. Mukherjee (2018)
Immunopathogenesis of Chlamydial Infections.
A. Murthy (2018)
Deconstructing the Chlamydial Cell Wall.
A. Klöckner (2018)
The bacteria : Intracellular life cycle , Chlamydia trachomatis serovars and virulence factors
A. Luján (2018)
Chlamydia trachomatis: the Persistent Pathogen
S. Witkin (2017)
Productive and Penicillin-Stressed Chlamydia pecorum Infection Induces Nuclear Factor Kappa B Activation and Interleukin-6 Secretion In Vitro
C. Leonard (2017)
Female Infertility Associated to Chlamydia trachomatis Infection
A. Luján (2016)
MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells
S. Storrie (2016)
The role of regulatory T and B cells in host responses to Chlamydia genital tract infection
J. Moore-Connors (2016)
A sinalização via NOD2-RIP2 modula a imunidade adaptativa contra Leishmania infantum
Manuela Sales Lima Nascimento (2016)
Sensing the enemy, containing the threat: cell-autonomous immunity to Chlamydia trachomatis.
Ryan Finethy (2016)
The role of NOD1 and NOD2 in host defense against chlamydial infection.
Y. Zou (2016)
The Biology of Spondyloarthritis: Studies of the host immune response in experimental Chlamydia infection and in patients with ankylosing spondylitis
Robert Eric Gracey (2016)
Inflammasome activation in ruminant cells infected with Chlamydia abortus
L. Doull (2016)
The role of integrins and human IDO in the immune response to Chlamydia trachomatis
Sergio J. Davila (2016)
Late production of CXCL8 in ruminant oro-nasal turbinate cells in response to Chlamydia abortus infection.
L. Doull (2015)
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