← Back to Search
Calcium-calmodulin Is Required For Abscisic Acid-induced Antioxidant Defense And Functions Both Upstream And Downstream Of H2 O2 Production In Leaves Of Maize (Zea Mays) Plants.
X. Hu, M. Jiang, J. Zhang, A. Zhang, F. Lin, M. Tan
Published 2007 · Medicine, Biology
Download PDFAnalyze on Scholarcy
• Using pharmacological and biochemical approaches, the role of calmodulin (CaM) and the relationship between CaM and hydrogen peroxide (H2 O2 ) in abscisic acid (ABA)-induced antioxidant defense in leaves of maize (Zea mays) plants were investigated. • Treatment with ABA or H2 O2 led to significant increases in the concentration of cytosolic Ca2+ in the protoplasts of mesophyll cells and in the expression of the calmodulin 1 (CaM1) gene and the content of CaM in leaves of maize plants, and enhanced the expression of the antioxidant genes superoxide dismutase 4 (SOD4), cytosolic ascorbate peroxidase (cAPX), and glutathione reductase 1 (GR1) and the activities of the chloroplastic and cytosolic antioxidant enzymes. The up-regulation of the antioxidant enzymes was almost completely blocked by pretreatments with two CaM antagonists. • Pretreatments with CaM antagonists almost completely inhibited ABA-induced H2 O2 production throughout ABA treatment, but pretreatment with an inhibitor or scavenger of reactive oxygen species (ROS) did not affect the initial increase in the contents of CaM induced by ABA. • Our results suggest that Ca2+ -CaM is involved in ABA-induced antioxidant defense, and that cross-talk between Ca2+ -CaM and H2 O2 plays a pivotal role in ABA signaling.
This paper references
Abscisic Acid and Antioxidant Defense in Plant Cells
Jiang Ming-yi (2004)
Protection against Heat Stress-Induced Oxidative Damage in Arabidopsis Involves Calcium, Abscisic Acid, Ethylene, and Salicylic Acid
Jane Larkindale (2002)
Cross-talk between calcium and reactive oxygen species originated from NADPH oxidase in abscisic acid-induced antioxidant defence in leaves of maize seedlings.
M. Jiang (2003)
Cis-elements and trans-factors that regulate expression of the maize Cat1 antioxidant gene in response to ABA and osmotic stress: H2O2 is the likely intermediary signaling molecule for the response.
L. Guan (2000)
Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweetpotato and its expression in response to stress
S.-Y. Park (2004)
Two structurally similar maize cytosolic superoxide dismutase genes, Sod4 and Sod4A, respond differentially to abscisic acid and high osmoticum.
L. Guan (1998)
Effect of Calcium and Calmodulin on Intrinsic Heat Tolerance in Relation to Antioxidant Systems in Maize Seedlings
M. Gong (1997)
Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves
X. Hu (2005)
A Plant Homolog of the Neutrophil NADPH Oxidase gp91phox Subunit Gene Encodes a Plasma Membrane Protein with Ca2+ Binding Motifs
T. Keller (1998)
Oxidative stress, antioxidants and stress tolerance.
R. Mittler (2002)
Calcium/Calmodulin Up-regulates a Cytoplasmic Receptor-like Kinase in Plants*
T. Yang (2004)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Hydrogen peroxide is necessary for abscisic acid-induced senescence of rice leaves.
K. Hung (2004)
Drought-Induced Changes in the Redox State of α-Tocopherol, Ascorbate, and the Diterpene Carnosic Acid in Chloroplasts of Labiatae Species Differing in Carnosic Acid Contents1
S. Munné-Bosch (2003)
Superoxide production by plant homologues of the gp91(phox) NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection.
M. Sagi (2001)
Extracellular Calmodulin-Induced Stomatal Closure Is Mediated by Heterotrimeric G Protein and H2O21
Yu-Ling Chen (2004)
Calcium/calmodulin-mediated signal network in plants.
T. Yang (2003)
Superoxide production by plant homologues of the gp91phox NADPH oxidase
M Sagi (2001)
Involvement of calcium-dependent protein kinase in rice (Oryza sativa L.) lamina inclination caused by brassinolide.
G. Yang (2000)
Reactive oxygen gene network of plants.
R. Mittler (2004)
Plant-specific calmodulin-binding proteins.
N. Bouché (2005)
The role of calcium and activated oxygens as signals for controlling cross-tolerance.
C. Bowler (2000)
Abscisic acid-induced apoplastic
X Hu (2005)
Determination of intracellular Ca2+ in cells of intact wheat roots: loading of acetoxymethyl ester of Fluo-3 under low temperature
W. Zhang (1998)
Expression of antioxidant enzymes in response to abscisic acid and high osmoticum in tobacco BY-2 cell cultures
P. Bueno (1998)
PCR-generated cDNA library of transition-stage maize embryos: cloning and expression of calmodulin genes during early embryogenesis
C. Breton (2004)
Involvement of plasma-membrane NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense in leaves of maize seedlings
M. Jiang (2002)
Hydrogen peroxide signalling.
S. Neill (2002)
Mitogen-Activated Protein Kinase Is Involved in Abscisic Acid-Induced Antioxidant Defense and Acts Downstream of Reactive Oxygen Species Production in Leaves of Maize Plants1
A. Zhang (2006)
Ca2+/calmodulin is critical for brassinosteroid biosynthesis and plant growth
L. Du (2005)
NADPH oxidase AtrbohD and AtrbohF genes function in ROS‐dependent ABA signaling in Arabidopsis
J. Kwak (2003)
Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway.
M. Orozco-Cárdenas (1999)
Hydrogen peroxide homeostasis: Activation of plant catalase by calcium/calmodulin
T. Yang (2002)
Calmodulin Is Involved in Heat Shock Signal Transduction in Wheat1
H. Liu (2003)
Calmodulin as a versatile calcium signal transducer in plants
W. Snedden (2001)
Abscisic acid-induced thermotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems
M. Gong (1998)
Transgenic tobacco expressing a foreign calmodulin gene shows an enhanced production of active oxygen species
S. Harding (1997)
Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves.
M. Jiang (2002)
Ca2+ influx into lily pollen grains through a hyperpolarization-activated Ca2+-permeable channel which can be regulated by extracellular CaM.
Zhong-lin Shang (2005)
Imaging of photo-oxidative stress responses in leaves.
M. Fryer (2002)
Oxidative Stress-Induced Calcium Signaling in Arabidopsis1
Maike C. Rentel (2004)
Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Zhen-Ming Pei (2000)
Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings.
M. Jiang (2001)
The Effects of Extracellular Calmodulin on Cell Wall Regeneration of Protoplasts and Cell Division
D. Sun (1995)
Calmodulin-binding protein kinases in plants.
L. Zhang (2003)
Regulation of the Arabidopsis transcriptome by oxidative stress.
R. Desikan (2001)
This paper is referenced by
Interactions between abscisic acid, hydrogen peroxide and nitric oxide mediate survival responses during water stress.
S. Neill (2007)
The pepper calmodulin gene CaCaM1 is involved in reactive oxygen species and nitric oxide generation required for cell death and the defense response.
Hyong Woo Choi (2009)
Abscisic acid in the plants-pathogen interaction
I. Maksimov (2009)
Differential expression of rice calmodulin promoters in response to stimuli and developmental tissue in transgenic tobacco plants.
K. Y. Jung (2010)
Melatonin improves rice salinity stress tolerance by NADPH oxidase-dependent control of the plasma membrane K+ transporters and K+ homeostasis.
Juan Liu (2020)
Isolation and Characterization of Calmodulin Gene from Panax ginseng C. A. Meyer
Neha G. Wasnik (2009)
Resistance to Aspergillus flavus in maize and peanut: Molecular biology, breeding, environmental stress, and future perspectives
Jake C. Fountain (2015)
Identity of an ABA-activated 46 kDa mitogen-activated protein kinase from Zea mays leaves: partial purification, identification and characterization
Haidong Ding (2009)
Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress
C. Shan (2015)
Salt-induced hydrogen peroxide is involved in modulation of antioxidant enzymes in cotton
Y. Wang (2016)
Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves.
A. Zhang (2007)
Interrelationship between calmodulin (CaM) and H2O2 in abscisic acid-induced antioxidant defense in the seedlings of Panax ginseng
Shohana Parvin (2012)
cGMP-dependent ABA-induced stomatal closure in the ABA-insensitive Arabidopsis mutant abi1-1.
L. Dubovskaya (2011)
Silicon Induced Antioxidative Responses and Expression of BOR2 and Two PIP Family Aquaporin Genes in Barley Grown Under Boron Toxicity
U. Çelikkol Akçay (2015)
Calcium as a versatile plant signal transducer under soil water stress.
S. Hong-bo (2008)
Functional Mechanism of Calmodulin for Cellular Responses in Plants
E. Cho (2009)
Mycorrhizal-induced calmodulin mediated changes in antioxidant enzymes and growth response of drought-stressed trifoliate orange
Y. Huang (2014)
Cross-talks between Ca2+/CaM and H2O2 in abscisic acid-induced antioxidant defense in leaves of maize plants exposed to water stress
X. Hu (2008)
Hydrogen peroxide as a signalling molecule in plants and its crosstalk with other plant growth regulators under heavy metal stress.
Faroza Nazir (2020)
Hormonal changes during salinity-induced leaf senescence in tomato (Solanum lycopersicum L.)
M. E. Ghanem (2008)
Ca2+ and CaM are Involved in NO- and H2O2-Induced Adventitious Root Development in Marigold
Weibiao Liao (2011)
Heat-Stress Physiology and Management
M. DaCosta (2013)
Plasma membrane Ca²+ transporters mediate virus-induced acquired resistance to oxidative stress.
S. Shabala (2011)
ROS and calcium signaling mediated pathways involved in stress responses of the marine microalgae Dunaliella salina to enhanced UV-B radiation.
X. Zhang (2017)
Exogenous calcium induces tolerance to atrazine stress in Pennisetum seedlings and promotes photosynthetic activity, antioxidant enzymes and psbA gene transcripts.
K. O. Erinle (2016)
Cloning and expression analysis of 14 lipid transfer protein genes from Tamarix hispida responding to different abiotic stresses.
C. Wang (2009)
Hydrogen sulfide is involved in the regulation of ascorbate-glutathione cycle by exogenous ABA in wheat seedling leaves under osmotic stress
C. Shan (2017)
The interaction between H2O2 and NO, Ca2+, cGMP, and MAPKs during adventitious rooting in mung bean seedlings
Shi-weng Li (2009)
Growth Responses and Leaf Antioxidant Metabolism of Grass Pea (Lathyrus sativus L.) Genotypes under Salinity Stress
D. Talukdar (2013)
Insects feeding on plants: rapid signals and responses preceding the induction of phytochemical release.
M. Maffei (2007)
Advances of calcium signals involved in plant anti-drought.
H. Shao (2008)
Integration of ROS and Hormone Signaling
I. Mori (2009)See more