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Heterotrophic Prokaryotic Production In Ultraoligotrophic Alpine Karst Aquifers And Ecological Implications.

I. Wilhartitz, A. Kirschner, H. Stadler, G. Herndl, M. Dietzel, C. Latal, R. Mach, A. Farnleitner
Published 2009 · Biology, Medicine

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Spring waters from alpine karst aquifers are important drinking water resources. To investigate in situ heterotrophic prokaryotic production and its controlling factors, two different alpine karst springs were studied over two annual cycles. Heterotrophic production in spring water, as determined by [(3)H]leucine incorporation, was extremely low ranging from 0.06 to 6.83 pmol C L(-1) h(-1) (DKAS1, dolomitic-karst-spring) and from 0.50 to 75.6 pmol C L(-1) h(-1) (LKAS2, limestone-karst-spring). Microautoradiography combined with catalyzed reporter deposition-FISH showed that only about 7% of the picoplankton community took up [(3)H]leucine, resulting in generation times of 3-684 days. Principal component analysis, applying hydrological, chemical and biological parameters demonstrated that planktonic heterotrophic production in LKAS2 was governed by the respective hydrological conditions, whereas variations in DKAS1 changed seemingly independent from discharge. Measurements in sediments recovered from LKAS2, DKAS1 and similar alpine karst aquifers (n=12) revealed a 10(6)-fold higher heterotrophic production (average 19 micromol C dm(-3) h(-1)) with significantly lower generation times as compared with the planktonic fraction, highlighting the potential of surface-associated communities to add to self-purification processes. Estimates of the microbially mediated CO(2) in this compartment indicated a possible contribution to karstification.
This paper references
10.1007/s002489900031
A Seasonal Study of Bacterial Community Succession in a Temperate Backwater System, Indicated by Variation in Morphotype Numbers, Biomass, and Secondary Production
A. Kirschner (2014)
10.1130/2006.2404(14)
Conductivity and sediment variation during storms as evidence of pathways to karst springs
L. Toran (2006)
Light Dependence of [H]Leucine Incorporation in the Oligotrophic North Pacific Ocean†
M. Church (2004)
Ausgewählte Methoden der Wasseruntersuchung, Vols 1 and 2. Fischer Verlag, Jena
C Legler (1988)
10.1016/S0065-2164(08)70206-5
Microbial ecology of the terrestrial subsurface.
W. Ghiorse (1988)
10.1128/AEM.49.3.599-607.1985
Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems.
D. Kirchman (1985)
Biomonitoring of groundwater systems: methods, applications and possible indicators among the groundwater biota. Groundwater Ecology – A Tool for Management of Water Resources (Griebler C, Danielopol
F Mösslacher (2001)
10.1128/AEM.70.7.4079-4087.2004
Light Dependence of [3H]Leucine Incorporation in the Oligotrophic North Pacific Ocean
M. Church (2004)
Protein content and protein synthesis rates of planctonic marine bacteria
M Simon (1989)
10.1007/S10040-008-0350-X
Microbial communities in karst groundwater and their potential use for biomonitoring
M. Pronk (2009)
10.1017/S0376892903000109
Present state and future prospects for groundwater ecosystems
D. Danielopol (2003)
und Hochschwab) und ihre Anwendung aus hydrologisch-ökologischer Sicht
T Dirnböck (1999)
10.1128/AEM.33.5.1225-1228.1977
Use of nuclepore filters for counting bacteria by fluorescence microscopy.
J. Hobbie (1977)
10.1029/2002GB001947
Influence of bacterial uptake on deep‐ocean dissolved organic carbon
J. Bendtsen (2002)
10.1128/AEM.68.6.3094-3101.2002
Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for the Identification of Marine Bacteria
A. Pernthaler (2002)
10.1128/AEM.64.2.431-438.1998
Significance of Viral Lysis and Flagellate Grazing as Factors Controlling Bacterioplankton Production in a Eutrophic Lake
M. Weinbauer (1998)
10.1046/J.1365-2427.2001.00741.X
Life on the edge: the plankton and chemistry of Beaver Lake, an ultra‐oligotrophic epishelf lake, Antarctica
Johanna Laybourn-Parry (2001)
Biomonitoring of groundwater systems: methods, applications and possible indicators among the groundwater biota
F Mösslacher (2001)
Karst springs, groundwater and surface runoff in the calcareous Alps: assessing quality and reliance of long-term water supply
K. Decker (1998)
Biofilme in Grundwasser-Ö kosystemen
C Griebler (1999)
10.1111/J.1574-6976.1994.TB00108.X
Microbial oxidation and reduction of manganese: consequences in groundwater and applications.
A. M. Gounot (1994)
10.1016/B978-0-08-057439-4.50015-0
11 – THE INORGANIC CARBON COMPLEX
R. Wetzel (2001)
10.1016/S0944-5013(99)80027-5
Myxobacteria in cave sediments of the French Jura Mountains
B. Menne (1999)
The relationship between biomass and volume of bacteria. Handbook of Aquatic Microbial Ecology (Kemp PF
S Norland (1993)
10.1016/0022-1694(77)90080-4
Carbon dioxide in the atmosphere of the unsaturated zone: An important control of groundwater hardness in limestones
T. Atkinson (1977)
10.1007/S10040-006-0108-2
Mineralogy of suspended sediment in three karst springs
Ellen K. Herman (2007)
10.1016/J.WATRES.2007.10.011
Bacterial carbon utilization in vertical subsurface flow constructed wetlands.
A. Tietz (2008)
10.1021/ES061129X
Hydrodynamic and microbial processes controlling nitrate in a fissured-porous karst aquifer of the Franconian Alb, southern Germany.
F. Einsiedl (2006)
10.1128/AEM.68.1.356-364.2002
Phylogenetic Identification and Substrate Uptake Patterns of Sulfate-Reducing Bacteria Inhabiting an Oxic-Anoxic Sewer Biofilm Determined by Combining Microautoradiography and Fluorescent In Situ Hybridization
T. Ito (2002)
10.1007/BF02540231
Comparison of bacteria from deep subsurface sediment and adjacent groundwater
T. Hazen (2006)
10.1111/j.1365-2672.2007.03319.x
Prokaryotic community analysis with CARD‐FISH in comparison with FISH in ultra‐oligotrophic ground‐ and drinking water
I. Wilhartitz (2007)
10.1130/0091-7613(1985)13<822:OOCAOS>2.0.CO;2
Origin of caves and other solution openings in the unsaturated (vadose) zone of carbonate rocks: A model for CO2 generation
W. Wood (1985)
Comparative physiology of biofilm and suspended organisms in the groundwater environment
Jm amp Kölbel-Boelke (1989)
10.1038/ismej.2008.18
Viruses in granitic groundwater from 69 to 450 m depth of the Äspö hard rock laboratory, Sweden
J. E. Kyle (2008)
10.3354/AME020001
Breakdown and microbial uptake of marine viruses and other lysis products
R. Noble (1999)
10.4319/LO.2003.48.1.0168
Contribution of major bacterial groups to bacterial biomass production (thymidine and leucine incorporation) in the Delaware estuary
M. Cottrell (2003)
10.1007/S002540050464
A model of early evolution of karst conduits affected by subterranean CO2 sources
F. Gabrovšek (2000)
10.5860/choice.26-2715
Geomorphology and Hydrology of Karst Terrains
W. White (1988)
10.1080/01490459409377983
Microbiological activity in thermoglacial karst springs, south spitsbergen
Stein‐Erik Lauritzen (1994)
10.1111/J.1365-2427.2008.02013.X
Microbial biodiversity in groundwater ecosystems
C. Griebler (2009)
10.7202/705154AR
Studies of BDOC and bacterial dynamics in the drinking water distribution system of the Northern Parisian suburbs
P. Servais (1992)
10.1007/s00248-002-2036-6
Incorporation of Radiolabeled Leucine into Protein to Estimate Bacterial Production in Plant Litter, Sediment, Epiphytic Biofilms, and Water Samples
N. Buesing (2002)
10.4319/LO.2006.51.1.0060
Archaeal uptake of enantiomeric amino acids in the meso- and bathypelagic waters of the North Atlantic
E. Teira (2006)
10.1016/S0022-1694(00)00324-3
Transport of free and particulate-associated bacteria in karst
B. Mahler (2000)
10.1128/AEM.66.11.4829-4833.2000
Marine Planktonic Archaea Take Up Amino Acids
C. Ouverney (2000)
10.2307/1313569
Viruses and Nutrient Cycles in the Sea Viruses play critical roles in the structure and function of aquatic food webs
S. Wilhelm (1999)
10.1016/0167-7012(95)00017-F
Methods for the study of rock-inhabiting microorganisms—A mini review
P. Hirsch (1995)
Viruses in granitic groundwater from 69 to 450 m depth of the Ä spö hard rock laboratory
J E Kyle (2008)
10.4319/LO.2004.49.1.0125
Influence of inlet bacteria on bacterioplankton assemblage composition in lakes of different hydraulic retention time
E. Lindström (2004)
Spatial and temporal patterns of bacterial density and metabolic activity in karst
KS Simon (2001)
10.3354/AME028117
Distribution patterns of attached and suspended bacteria in pristine and contaminated shallow aquifers studied with an in situ sediment exposure microcosm
C. Griebler (2002)
10.1128/AEM.66.2.455-466.2000
Limitation of Bacterial Growth by Dissolved Organic Matter and Iron in the Southern Ocean
M. Church (2000)
10.1111/j.0022-3646.1994.00807.x
THE IMPACT OF STORM‐FLOW ON RIVER BIOFILM ARCHITECTURE 1
S. Blenkinsopp (1994)
10.1016/0022-1694(83)90234-2
The role of the subcutaneous zone in karst hydrology
P. W. Williams (1983)
10.3354/MEPS051201
Protein content and protein synthesis rates of planktonic marine bacteria
M. Simon (1989)
10.1007/BF00170249
Density, activity, and diversity of bacteria indigenous to a karstic aquifer
K. Rusterholtz (2004)
10.3354/AME017211
Material fluxes through the procaryotic compartment in a eutrophic backwater branch of the River Danube
A. Kirschner (1999)
10.1007/S10040-003-0299-8
Storm pulse chemographs of saturation index and carbon dioxide pressure: implications for shifting recharge sources during storm events in the karst aquifer at Fort Campbell, Kentucky/Tennessee, USA
D. Vesper (2004)
10.1007/S00254-003-0899-6
Spring and conduit sediments as storage reservoirs for heavy metals in karst aquifers
D. Vesper (2004)
10.1007/s00248-002-2041-9
Factors Controlling Extremely Productive Heterotrophic Bacterial Communities in Shallow Soda Pools
A. Eiler (2003)
10.1128/aem.65.7.3264-3264.1999
Combined Microautoradiography–16S rRNA Probe Technique for Determination of Radioisotope Uptake by Specific Microbial Cell Types In Situ
C. Ouverney (1999)
10.1007/S10040-005-0009-9
Review: Microbial biocenoses in pristine aquifers and an assessment of investigative methods
Nicola Goldscheider (2006)
10.1128/AEM.00763-06
Quantifying Substrate Uptake by Individual Cells of Marine Bacterioplankton by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization Combined with Microautoradiography
E. Sintes (2006)
10.1007/S10040-005-0454-5
Dynamics and interaction of organic carbon, turbidity and bacteria in a karst aquifer system
M. Pronk (2006)
10.1016/J.JHYDROL.2007.12.001
Threshold events in spring discharge: Evidence from sediment and continuous water level measurement
E. K. Herman (2008)
10.1007/BF00662027
Comparison between geochemical and biological estimates of subsurface microbial activities
T. Phelps (2004)
10.1111/J.1462-2920.2005.00810.X
Bacterial dynamics in spring water of alpine karst aquifers indicates the presence of stable autochthonous microbial endokarst communities.
A. Farnleitner (2005)
10.1016/0012-8252(93)90058-F
The deep subterranean biosphere
K. Pedersen (1993)
10.1002/IROH.200390025
The choice of standardisation reveals a significant influence on the dynamics of bacterial abundance in newly deposited River sediments
A. H. Farnleitner (2003)
10.1016/B978-0-08-050762-0.50023-1
16 – Dynamics of Communities and Ecology of Karst Ecosystems: Example of Three Karsts in Eastern and Southern France
J. Gibert (1994)
10.2307/j.ctvh1dhd8
Life On The Edge
M. Gross (1998)
10.1023/B:RESB.0000040464.52512.44
Use of Microautoradiography to Study in situ Physiology of Bacteria in Biofilms
J. Nielsen (2003)
GEOLOGISCHE KARTIERUNG IM HOCHSCHWABGEB IET- ENTSCHEIDUNGSHILFE ZUR ABGRENZUNG VON QUELLEINZUGSGEBIETEN
Gerhard Bryda (2001)
10.4319/LO.2006.51.3.1262
Prokaryotic respiration and production in the meso- and bathypelagic realm of the eastern and western North Atlantic basin
T. Reinthaler (2006)
Dynamics of communities and ecology of Karst ecosystems: example of three Karsts in Eastern and South France. Groundwater Ecology
J Gibert (1994)
Karstwasserdynamik und Karstwasserschutz Hochschwab STA 28K/96. Enbericht 2. Arbeitsjahr im Auftrag MA31 Stadt Wien
H Stadler (1998)
10.1021/ES050426J
Sources and processes affecting sulfate in a karstic groundwater system of the Franconian Alb, southern Germany.
F. Einsiedl (2005)
10.1128/AEM.70.7.4411-4414.2004
Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean
E. Teira (2004)
The inorganic carbon complex
RG Wetzel (1991)
Ausgewählte Methoden der Wasseruntersuchung, Vols 1 and 2
C Legler (1988)
Microbial ecology of the subsurface. Groundwater Ecology -A Tool for Management of Water Resources
C Griebler (2001)
10.1127/ARCHIV-HYDROBIOL/151/2001/67
Spatial and temporal patterns of bacterial density and metabolic activity in a karst aquifer
K. Simon (2001)
10.1016/J.GEOMORPH.2004.07.008
Weathering, geomorphic work, and karst landscape evolution in the Cave City groundwater basin, Mammoth Cave, Kentucky
C. Groves (2005)
10.1016/S0043-1354(96)00311-9
Groundwater samples do not reflect bacterial densities and activity in subsurface systems
A. Alfreider (1997)
10.1016/J.FEMSRE.2003.08.001
Ecology of prokaryotic viruses.
Markus G. Weinbauer (2004)
10.1046/J.1462-2920.2003.00402.X
Quantification of cell-specific substrate uptake by probe-defined bacteria under in situ conditions by microautoradiography and fluorescence in situ hybridization.
J. Nielsen (2003)
10.1002/JOBM.19750150517
CHRISTA LEGLER (Redaktion), Ausgewählte Methoden der Wasseruntersuchung) Band I: Chemische, physikalisch‐chemische, physikalische und elektrochemische Methoden, Lieferung 2. 143 S., 5 Abb., 4 Tab. Jena 1973: VEB Gustav Fischer. M 17,00
A. Schwartz (1975)
10.1007/978-94-009-7944-4_45
Microbial Oxidation and Reduction of Manganese and Iron
K. Nealson (1983)
Karstwasserdynamik und Karstwasserschutz Hochschwab STA 28K/96
H Stadler (1998)
Microbial oxidation and reduction
AM Gounot (1994)
Karstwasserdynamik Zeller Staritzen
H Stadler (1997)



This paper is referenced by
10.1051/LIMN/2011057
Bryophyte communities and seston in a karst stream (Jankovac Stream, Papuk Nature Park, Croatia)
M. Špoljar (2012)
10.1016/j.jenvman.2015.09.039
Impacts of management and climate change on nitrate leaching in a forested karst area.
T. Dirnböck (2016)
10.3390/w12123343
Investigating the Structure of a Coastal Karstic Aquifer through the Hydrogeological Characterization of Springs Using Geophysical Methods and Field Investigation, Gökova Bay, SW Turkey
Çağdaş Sağir (2020)
10.2166/wst.2010.500
The spectral absorption coefficient at 254 nm as a real-time early warning proxy for detecting faecal pollution events at alpine karst water resources.
H. Stadler (2010)
10.1016/j.jconhyd.2017.10.004
Response and recovery of a pristine groundwater ecosystem impacted by toluene contamination - A meso-scale indoor aquifer experiment.
Agnieszka Herzyk (2017)
10.2166/wst.2014.032
A sensitive and robust method for automated on-line monitoring of enzymatic activities in water and water resources.
G. Ryzinska-Paier (2014)
Spatial and temporal dynamics of microbial activities in a tar oil contaminated aquifer
A. Bayer (2012)
10.3389/fmicb.2018.02823
High Microbial Diversity Despite Extremely Low Biomass in a Deep Karst Aquifer
Olivia S. Hershey (2018)
Sources of CO 2 Controlling the Carbonate Chemistry of the Logsdon River, Mammoth Cave,
B. Elliott (2013)
10.3389/fmicb.2019.00028
Spring Water of an Alpine Karst Aquifer Is Dominated by a Taxonomically Stable but Discharge-Responsive Bacterial Community
D. Savio (2019)
Sources of CO 2 Controlling the Carbonate Chemistry of the Logsdon River, Mammoth Cave, Kentucky
B. Hatcher (2013)
10.1016/j.copbio.2014.01.018
Current developments in groundwater ecology--from biodiversity to ecosystem function and services.
C. Griebler (2014)
10.1016/J.JNGSE.2017.12.031
Diagenesis of microbialites in the lower Cambrian Qingxudong Formation, South China: Implications for the origin of porosity in deep microbial carbonates
Q. Tan (2018)
10.1007/978-1-4419-9386-1_18
Agricultural and Rural Watersheds
A. Farnleitner (2011)
Spatio-temporal patterns of suspended and attached bacterial communities in a hydrologically dynamic aquifer (Mittenwald, Germany)
Yuxiang Zhou (2013)
10.1007/978-981-15-0679-6_1
Microbial Ecological Function in Migration of Radionuclides in Groundwater
K. Kato (2020)
10.4314/WSA.V35I4.76809
Heterotrophic plate count vs. in situ bacterial 16S rRNA gene amplicon profiles from drinking water reveal completely different communities with distinct spatial and temporal allocations in a distribution net.
M. Burtscher (2009)
10.1111/jdv.16599
In‐depth prospection of Avène Thermal Spring Water reveals an uncommon and stable microbial community
M. Bourrain (2020)
10.1016/j.resmic.2015.12.006
Spatial and temporal variability of bacterial communities in high alpine water spring sediments.
A. Esposito (2016)
10.1111/jeu.12054
Phagotrophic Protist Diversity in the Groundwater of a Karstified Aquifer – Morphological and Molecular Analysis
Ute Risse-Buhl (2013)
10.1016/j.envpol.2012.10.014
Occurrence and dynamics of micropollutants in a karst aquifer.
B. Morasch (2013)
10.1007/978-3-319-51070-5_20
Integrated Strategy to Guide Health-Related Microbial Quality Management at Alpine Karstic Drinking Water Resources
A. H. Farnleitner (2018)
10.1002/wat2.1282
Opening the black box of spring water microbiology from alpine karst aquifers to support proactive drinking water resource management
D. Savio (2018)
10.1111/1462-2920.12124
Mass effects meet species sorting: transformations of microbial assemblages in epiphreatic subsurface karst water pools.
Tanja Shabarova (2013)
10.1111/j.1365-2672.2010.04788.x
Escherichia coli and enterococci are sensitive and reliable indicators for human, livestock and wildlife faecal pollution in alpine mountainous water resources
A. H. Farnleitner (2010)
10.3354/AME01862
DOM and bacterial growth efficiency in oligotrophic groundwater: absence of priming and co-limitation by organic carbon and phosphorus
R. Hofmann (2017)
10.1016/j.scitotenv.2014.10.031
Biofilm resilience to desiccation in groundwater aquifers: a laboratory and field study.
L. Weaver (2015)
10.3390/W10081072
Bacterial Productivity in a Ferrocyanide-Contaminated Aquifer at a Nuclear Waste Site
A. E. Plymale (2018)
10.1021/es103659s
Hypothesis-Driven Approach for the Identification of Fecal Pollution Sources in Water Resources
G. Reischer (2011)
10.1080/01490451.2014.991811
Microbes in Groundwater of a Volcanic Mountain, Mt. Fuji; 16S rDNA Phylogenetic Analysis as a Possible Indicator for the Transport Routes of Groundwater
T. Segawa (2015)
10.1016/J.JHYDROL.2010.07.029
Comment on ``Non-permanent shallow halocline in a fractured carbonate aquifer, southern Italy'' by E. Petrella, G. Naclerio, A. Falasca, A. Bucci, P. Capuano, V. De Felice and F. Celico [J. Hydrol. 373 (2009) 267-272]
I. Wilhartitz (2010)
10.1111/j.1574-6941.2012.01371.x
Spatio-temporal patterns of microbial communities in a hydrologically dynamic pristine aquifer.
Yuxiang Zhou (2012)
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