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Photodynamic Treatment Of Chaoborus Crystallinus Larvae With Chlorophyllin Induces Necrosis And Apoptosis.

S. Wohllebe, C. Ulbrich, D. Grimm, J. Pietsch, G. Erzinger, R. Richter, M. Lebert, P. Richter, Donat-Peter Häder
Published 2011 · Medicine, Chemistry

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Chlorophyllin kills mosquito larvae (Culex, Aedes) in the aquatic habitat at low concentrations via photodynamic reactions under irradiation. The effects of chlorophyllin were investigated at the cellular level using the transparent larvae of Chaoborus crystallinus as a model system. Their transparency enabled in situ fluorescence investigation, showing that chlorophyllin accumulates in the intestine of the larvae. Uptake of chlorophyllin at room temperature took about 2 h. The fluorescence signal peaked after 5 h of incubation. Chlorophyllin accumulates up to about 15 ng per larvae. The intestine of treated larvae was dissected and stained with several dyes (acridine orange, Hoechst 33342 and propidium iodide). Apoptosis and necrosis increased with higher concentrations of chlorophyllin (to a smaller extent in dark controls) and were elevated in irradiated samples. Single cells from treated larvae were isolated and subjected to Annexin V flow cytometry. The fraction of apoptotic and necrotic cells increased significantly at a high chlorophyllin concentration (21.4 mg L(-1)) and under intensive irradiation. The activity of caspases-3, -8 and -9 as well as Bcl-2 and cytochrome c was investigated by means of western blot analysis. The data suggest a possible chlorophyllin concentration-dependent shift of the apoptotic pathway.
This paper references
10.1021/BC0602578
Peptide-based pharmacomodulation of a cancer-targeted optical imaging and photodynamic therapy agent.
Klara Stefflova (2007)
Chlorophyll and bacteriochlorophyll, their preparation and pharmaceutical compositions comprising them
A. Scherz (1993)
10.1182/blood.V89.2.630
Mcl-1, a Bcl-2 family member, delays the death of hematopoietic cells under a variety of apoptosis-inducing conditions.
P. Zhou (1997)
The role of reactive oxygen species (ROS) in signaling of light stress
P. Richter (2003)
10.1042/bst0110591
Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents
H. Lichtenthaler (1983)
10.1038/sj.bjc.6601457
Combined hyperthermia and chlorophyll-based photodynamic therapy: tumour growth and metabolic microenvironment
D. Kelleher (2003)
10.1269/JRR.45.181
Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation.
H. Tominaga (2004)
10.1016/J.JPHOTOBIOL.2004.05.005
Photoactivation of pheophorbide a induces a mitochondrial-mediated apoptosis in Jurkat leukaemia cells.
Won-young Lee (2004)
8-Sunlight and photosensitizers for insect control. The 9th International Conference on ‘‘Environmental Protection is a must,’
M. H. Abdel-Kader (1999)
10.1002/jcb.21710
Effects of basic fibroblast growth factor on endothelial cells under conditions of simulated microgravity
C. Ulbrich (2008)
10.3349/ymj.1989.30.3.212
Chlorophyll derivatives--a new photosensitizer for photodynamic therapy of cancer in mice.
Y. J. Park (1989)
Cancer therapy, enzymatic acclimation and molecular machines; the chlorophyll connection
A. Scherz (2006)
10.1111/j.1751-1097.1996.tb02439.x
Serine conjugates of chlorophyll and bacteriochlorophyll: photocytotoxicity in vitro and tissue distribution in mice bearing melanoma tumors.
V. Rosenbach-Belkin (1996)
Bekämpfung humanpathologischer Parasiten mittels natürlicher Photosensitizer. Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany
Wohllebe (2008)
10.1088/0031-9155/31/4/001
The physics of photodynamic therapy.
B. Wilson (1986)
10.1093/CARCIN/BGH331
Green vegetables, red meat and colon cancer: chlorophyll prevents the cytotoxic and hyperproliferative effects of haem in rat colon.
Johan de Vogel (2005)
Should DDT be used to combat Malaria? Sci
M. Cone (2009)
10.1078/0176-1617-00552
Mycosporine-like amino acids (MAAs) protect against UV-B-induced damage in Gyrodinium dorsum Kofoid
M. Klisch (2001)
10.1002/(SICI)1520-6866(1999)19:5<313::AID-TCM1>3.0.CO;2-G
Effect of dietary chlorophyll derivatives on mutagenesis and tumor cell growth.
S. Chernomorsky (1999)
10.1038/SCIENTIFICAMERICAN1110-68
Halting the world's most lethal parasite.
Mary Jane Carmichael (2010)
10.1002/9780470688618.TAW0166
History of Human Parasitology
D. F. Cox (2010)
10.1159/000303059
Characterization of Human Chondrocytes Exposed to Simulated Microgravity
C. Ulbrich (2010)
Bacteriochlorophyllserine generates only OH radicals under near-infrared illumination (NIR)
S. Katz (1998)
10.1073/pnas.202607599
The breast cancer resistance protein protects against a major chlorophyll-derived dietary phototoxin and protoporphyria
J. Jonker (2002)
10.1055/S-2005-873149
Pheophorbide a, a major antitumor component purified from Scutellaria barbata, induces apoptosis in human hepatocellular carcinoma cells.
J. Chan (2006)
10.1002/jcb.20932
Photodynamic cell‐kill analysis of breast tumor cells with a tamoxifen‐pyropheophorbide conjugate
Ana Fernandez Gacio (2006)
Photodynamic cellkill analysis of breast tumor cells with a tamoxifenpyropheophorbide conjugate
H. K. Lichtenthaler (2006)
DDT: A case study in scientific fraud
J. G. Edwards (2004)
10.1002/cyto.990170108
A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells.
F. Belloc (1994)
10.1007/s00436-011-2322-7
Optimizing conditions for the use of chlorophyll derivatives for photodynamic control of parasites in aquatic ecosystems
Gilmar Sidnei Erzinger (2011)
10.1038/415680a
The economic and social burden of malaria
J. Sachs (2002)
10.1007/s00436-008-1235-6
Photodynamic control of human pathogenic parasites in aquatic ecosystems using chlorophyllin and pheophorbid as photodynamic substances
S. Wohllebe (2008)
10.1046/J.1365-2168.1999.01132.X
Human pancreatic carcinoma cells are sensitive to photodynamic therapy in vitro and in vivo
A. Hajri (1999)
10.1562/0031-8655(2001)073<0257:ATOSMT>2.0.CO;2
Antivascular Treatment of Solid Melanoma Tumors with Bacteriochlorophyll–serine-based Photodynamic Therapy¶
Judith Zilberstein (2001)
10.1562/0031-8655(2002)0750644ptwpam2.0.co2
Photodynamic therapy with pyropheophorbide-a methyl ester in human lung carcinoma cancer cell: efficacy, localization and apoptosis.
X. Sun (2002)
10.1039/B606117F
Synthesis of estradiol-pheophorbide a conjugates: evidence of nuclear targeting, DNA damage and improved photodynamic activity in human breast cancer and vascular endothelial cells.
N. El-Akra (2006)
How does photodynamic therapy
B. W. Henderson (1992)
Caspase-8 and apoptosis-inducing factor mediate a cytochrome c-independent pathway of apoptosis in human colon cancer cells induced by the dietary phytochemical chlorophyllin.
G. Díaz (2003)
To control Malaria, we need DDT! 21st Century Fall, 1
D. R. Roberts (2002)
10.1016/S1011-1344(01)00278-0
Involvement of reactive oxygen species in the UV-B damage to the cyanobacterium Anabaena sp.
Y. Y. He (2002)
10.1111/j.1751-1097.1992.tb04222.x
How does photodynamic therapy work?
B. Henderson (1992)
10.1002/ijc.11002
Photodynamic therapy with Pd‐bacteriopheophorbide (TOOKAD): Successful in vivo treatment of human prostatic small cell carcinoma xenografts
N. Koudinova (2003)
Deworming young children
J. G. Edwards (2004)
10.1210/EN.2002-0171
Weightlessness induced apoptosis in normal thyroid cells and papillary thyroid carcinoma cells via extrinsic and intrinsic pathways.
P. Kossmehl (2003)



This paper is referenced by
Photodynamic inactivation of bacteria by cationic porphyrins : their cellular targets and potential environmental applications
Eliana Alves (2013)
10.1007/s00436-014-4175-3
Chlorophyll derivatives can be an efficient weapon in the fight against dengue
Azizullah Azizullah (2014)
10.1016/j.jphotobiol.2015.01.016
Toxicity of chlorophyllin in different wavelengths of visible light against Fasciola gigantica larvae.
Divya Jyoti Singh (2015)
10.1007/s00436-015-4884-2
Treatment of ichthyophthiriasis with photodynamically active chlorophyllin
D-P Häder (2015)
Therapeutic and pharmacological aspects of photodynamic product chlorophyllin
Divya Chaturvedi (2019)
10.1007/s10646-015-1437-5
Assessment of the impact of chlorophyll derivatives to control parasites in aquatic ecosystems
Gilmar Sidnei Erzinger (2015)
10.5530/PJ.2018.4.129
Photoactivated chlorophyllin and acetylcholinesterase/cytochrome oxidase activity in Fasciola gigantica cercaria larvae
Dinesh K Singh (2018)
10.1016/J.JPHOTOCHEMREV.2014.09.003
Potential applications of porphyrins in photodynamic inactivation beyond the medical scope
Eliana Alves (2015)
Assessment the Effect Of Photodynamic Chlorophyllin On Biochemical Changes In The Cerebral Ganglion Of Snail Lymnaea Acuminata
Divya Chaturvedi (2017)
10.1007/s00436-012-2893-y
Chlorophyllin for the control of Ichthyophthirius multifiliis (Fouquet)
Stephanie Wohllebe (2012)
10.3923/JP.2015.160.166
Anti-reproductive Activity of Chlorophyllin on Fresh Water Snail Lymnaea acuminata
Kavita Singh (2015)
10.3389/fenvs.2014.00018
Chlorophyllin as a possible measure against vectors of human parasites and fish parasites
Peter R. Richter (2014)
10.1007/s00436-016-4972-y
Fighting fish parasites with photodynamically active chlorophyllin
D-P Häder (2016)
10.1155/2017/5219194
Photomediated Larvicidal Activity of Pheophorbide a against Cercaria Larvae of Fasciola gigantica
Divya Jyoti Singh (2017)
10.1039/c9pp00156e
Mosquito larvae control by photodynamic inactivation of their intestinal flora - a proof of principal study on Chaoborus sp.
Annegret Preuß (2019)
10.4172/2153-2435.1000139
New Perspectives for the Control of Parasitic Diseases Through the Use of Photodynamic Products
Gilmar Sidnei Erzinger (2011)
10.3923/IJZR.2015.207.214
Bait Formulations of Chlorophyllin against Infected/Uninfected Lymnaea acuminata in Red and Sunlight
Navneet Kumar (2015)
10.21315/tlsr2016.27.2.3
Toxicity of Chlorophyllin against Lymnaea acuminata at Different Wavelengths of Visible Light.
Divya Chaturvedi (2016)
10.1016/J.MRGENTOX.2019.02.008
Radioprotective properties of food colorant sodium copper chlorophyllin on human peripheral blood cells in vitro.
Marko Gerić (2019)
10.5530/pj.2017.6.115
Photodynamic Toxicity of Chlorophyllin against Fasciola gigantica Carrier Snail Indoplanorbis exustus in Visible Spectral Band
Divya Chaturvedi (2017)
10.1590/S1678-9946201658039
ANTHELMINTIC ACTIVITY OF CHLOROPHYLLIN AGAINST DIFFERENT LARVAL STAGES OF Fasciola gigantica
Divya Jyoti Singh (2016)
10.17169/refubium-17130
Proteomanalyse humaner Schilddrüsenzellen kultiviert unter Einflusssimulierter Schwerelosigkeit
J. Pietsch (2012)
10.1016/J.EIAR.2014.09.011
Chlorophyll derivatives for pest and disease control: Are they safe?
Azizullah Azizullah (2015)
10.3923/JP.2016.27.32
Pheophorbide a Potential Source of Plant Molluscicide to Combat Against Neglected Tropical Disease Fasciolosis
Kavita Singh (2016)
eview otential applications of porphyrins in photodynamic inactivation eyond the medical scope
L. M. Alvesa (2015)
10.1016/j.parepi.2016.03.005
Phytotherapy of chlorophyllin exposed Lymnaea acuminata: A new biotechnological tool for fasciolosis control
Divya Jyoti Singh (2016)
10.5530/pj.2017.5.94
Chlorophyllin Treatment Against the Snail Lymnaea acuminata: A new tool in Fasciolosis Control
Kavita Singh (2017)
10.1007/978-3-030-50971-2
Pigments from Microalgae Handbook
E. Jacob-Lopes (2020)
10.12980/jclm.1.2013c824
Molluscicidal activity of chlorophyll extraction against the freshwater snails
M. S. Mahmoud (2013)
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