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Establishment Of An Efficient In Vitro Culture And Particle Bombardment‐mediated Transformation Systems In Miscanthus Sinensis Anderss., A Potential Bioenergy Crop

X. Wang, Tetsuya Yamada, Fanjiang Kong, Y. Abe, Y. Hoshino, H. Sato, T. Takamizo, A. Kanazawa, T. Yamada
Published 2011 · Biology

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Plants belonging to the genus Miscanthus are considered promising bioenergy crops. Here, we report the establishment of tissue culture system through particle bombardment‐mediated transformation in Miscanthus sinensis Anderss. Callus was induced efficiently from mature seeds in a medium containing a combination of a relatively high level of 2,4‐dichlorophenoxyacetic acid (5 mg L−1) and a relatively low level of 6‐benzyladenine (BA) (0.01 mg L−1). Callus induction potential of 18 accessions of M. sinensis, which were collected from various sites in Japan, was compared. Significant correlation was detected between compact (embryogenic) callus induction frequency and average annual air temperature in collection sites. An accession from Tanegashima Island showed the highest production of compact callus. We found that a higher level of BA causes callus browning; the 2 mg L−1 BA is the optimal concentration for regeneration. Both compact and friable calli were suitable for particle bombardment transformation. Through selection under the presence of 50 mg L−1 hygromycin for 3 weeks and further selection under the presence of 150 mg L−1 for 1 month, hygromycin‐resistant calli survived, of which 72.2% had been entirely transformed. Plants were regenerated from calli in the presence of hygromycin; transcripts of the hpt and gfp genes, which were cobombarded to the calli, were detected in the regenerated plants. This is the first report on the establishment of the in vitro culture of M. sinensis using mature seeds, the variation of callus formation among accessions collected from various sites in Japan, and particle bombardment‐mediated transformation in the genus Miscanthus.
This paper references
10.1111/J.1744-697X.2010.00195.X
Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass (Zoysia japonica Steud.)
X. Wang (2010)
10.1007/978-3-642-79048-5
Plant Cell, Tissue and Organ Culture
O. Gamborg (1995)
GENETIC TRANSFORMATION OF ELITE TURF-TYPE CULTIVARS OF TALL FESCUE
Y. Bai (2001)
A simple and rapid Agrobacterium
RK gar (2004)
10.1016/S0168-9452(97)00259-8
Transgenic plants of Lolium multiflorum, Lolium perenne, Festuca arundinacea and Agrostis stolonifera by silicon carbide fibre-mediated transformation of cell suspension cultures
S. Dalton (1998)
10.21273/HORTSCI.25.10.1291
In vitro propagation of Miscanthus sinensis.
N. Gawel (1990)
10.1023/A:1025848016557
Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis
Choun-Sea Lin (2004)
10.1046/J.1439-0523.2002.738314.X
In vitro chromosome doubling of Miscanthus sinensis
K. K. Petersen (2002)
10.1007/s00299-003-0734-2
Agrobacterium tumefaciens-mediated creeping bentgrass (Agrostis stolonifera L.) transformation using phosphinothricin selection results in a high frequency of single-copy transgene integration
H. Luo (2003)
Genetic improvement of C4
K Jakob (2009)
10.1007/s002990000238
Production of transgenic tall fescue and red fescue plants by particle bombardment of mature seed-derived highly regenerative tissues
M.-J. Cho (2000)
10.1007/s11627-009-9219-5
Genetic modification of lignin biosynthesis for improved biofuel production
H. Hisano (2009)
10.1007/BF00043427
Callus induction and plant regeneration from different explant types of Miscanthus x ogiformis Honda ‘Giganteus’
I. Holme (2004)
Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation.
Koichi Toyama (2003)
Miscanthus : European experience with a novel energy crop
I. Lewandowskia
Agrobacterium tumefaciens-mediated transformation of forage-type perennial ryegrass (Lolium perenne L.). Grassland Science
H Sato (2006)
10.1111/j.1757-1707.2010.01054.x
Miscanthus×giganteus plant regeneration: effect of callus types, ages and culture methods on regeneration competence
Hyoung Seok Kim (2010)
10.1023/A:1008693214629
Genetic diversity of European Miscanthus species revealed by AFLP fingerprinting
J. M. Greef (2004)
10.2172/4538
Miscanthus: A Review of European Experience with a Novel Energy Crop
J. Scurlock (1999)
10.1016/S0961-9534(03)00030-8
The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe
I. Lewandowski (2003)
Cold tolerance of C 4 photosynthesis in Miscanthus  giganteus: adaptation in amounts and sequence of C 4 photosynthetic enzymes
S L Naidu (2003)
10.1146/annurev.arplant.043008.092125
Cellulosic biofuels.
A. Carroll (2009)
10.1016/J.RSER.2008.09.006
The economical and environmental performance of miscanthus and switchgrass production and supply chains in a European setting
E. Smeets (2009)
10.1007/BF01281800
Somatic embryogenesis in sugarcane (Saccharum officinarum L.) I. The morphology and physiology of callus formation and the ontogeny of somatic embryos
Wai -Jane Ho (2005)
10.1007/s00299-002-0534-0
Expression of pokeweed antiviral proteins in creeping bentgrass
W. Dai (2002)
10.1016/J.PLANTSCI.2004.05.037
Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing
H. Hisano (2004)
10.1016/S0168-9452(02)00069-9
Cloning of a chitinase-like cDNA (hs2), its transfer to creeping bentgrass (Agrostis palustris Huds.) and development of brown patch (Rhizoctonia solani) disease resistant transgenic lines
Benli Chai (2002)
10.1023/A:1005808329685
Callus induction and plant regeneration in Miscanthus x ogiformis Honda 'Giganteus' as influenced by benzyladenine
K. K. Petersen (2004)
10.1046/J.1469-8137.2002.00381.X
Comparative responses to water stress in stay-green, rapid- and slow senescing genotypes of the biomass crop, Miscanthus
J. Clifton-Brown (2002)
10.1007/BF02319019
Callus friability and somatic embryogenesis inHevea brasiliensis
P. Montoro (2006)
10.1017/CBO9780511574528.017
Molecular Embryology of Flowering Plants: Somatic embryogenesis
V. Raghavan (1997)
10.1111/J.1601-5223.1993.00297.X
Cytogenetic Analysis of Miscanthus‘Giganteus’, an Interspecific Hybrid
I. Linde-Laursen (2004)
10.1111/J.1365-2486.2007.01461.X
Prediction of the distribution of Arctic-nesting pink-footed geese under a warmer climate scenario
R. A. Jensen (2007)
10.1007/s00299-003-0710-x
A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of transgenic plants
S. Leelavathi (2004)
10.1104/pp.103.021790
Cold Tolerance of C4 photosynthesis in Miscanthus × giganteus: Adaptation in Amounts and Sequence of C4 Photosynthetic Enzymes1
S. Naidu (2003)
10.1007/BF00039917
Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids)
P. Taylor (2004)
10.1016/0031-9422(80)85004-7
A rapid DNA isolation procedure for small quantities of fresh leaf tissue
E. Pahlich (1980)
10.1007/s11105-008-0032-9
Enhanced Agrobacterium-mediated Transformation of Embryogenic Calli of Upland Cotton via Efficient Selection and Timely Subculture of Somatic Embryos
Shen-Jie Wu (2008)
10.1023/B:MITI.0000038848.94134.BE
Miscanthus for Renewable Energy Generation: European Union Experience and Projections for Illinois
E. Heaton (2004)
10.1007/BF03195692
Genetic and nongenetic factors influencing callus induction inMiscanthus sinensis (Anderss.) anther cultures
K. Głowacka (2010)
10.1111/J.1365-2486.2008.01662.X
Meeting US biofuel goals with less land: the potential of Miscanthus
E. Heaton (2008)
10.1023/B:MOLB.0000012327.47625.23
Floral inhibition in red fescue (Festuca rubra L.) through expression of a heterologous flowering repressor from Lolium
C. Jensen (2004)
10.1023/A:1005981300847
Embryogenic callus formation, growth and regeneration in callus and suspension cultures of Miscanthus x ogiformis Honda Giganteus' as affected by proline
I. Holme (2004)
10.1111/J.1365-313X.1992.00409.X
Transgenic sugarcane plants via microprojectile bombardment
R. Bower (1992)
Callus induction and plant regen
IB 203–210. Holme (1996)
Somatic Embryogenesis: Molecular Embryology of Flowering Plants
V Raghavan (1997)
10.1007/BF00039961
Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application in Miscanthus x ogiformis ‘Giganteus’
K. Brandt (1995)
10.1016/J.INDCROP.2004.01.004
Establishing Miscanthus sinensis from seed using conventional sowing methods
D. Christian (2005)
10.1007/BF00051586
Synergism of thidiazuron and benzyladenine in axillary shoot formation depends on sequence of application in Miscanthus X ogiformis ‘Giganteus’
J. M. Nielsen (2004)
Miscanthus productivity
M Jones (2001)
A rapid DNA isolation procedure for small amounts of fresh leaf tissue
J. Doyle (1987)
Somatic embryogenesis in herbaceous monocots
SM Shukla (1995)
Somatic embryogenesis in herbaceous monocots
S KrishnaRaj (1995)
Rapid and efficient callus induction and plant regeneration from seeds of zoysiagrass
X Wang (2010)
10.1007/s11627-009-9214-x
Genetic improvement of C4 grasses as cellulosic biofuel feedstocks
Katrin Jakob (2009)
10.1023/A:1006348615717
Significance of different carbon sources and sterilization methods on callus induction and plant regeneration of Miscanthus x ogiformis Honda `Giganteus'
K. K. Petersen (2004)
10.1111/J.1365-2486.2007.01438.X
Carbon mitigation by the energy crop, Miscanthus
John Clifton-Brown (2007)
10.1023/A:1026589804034
Generation of large numbers of independently transformed fertile perennial ryegrass (Lolium perenne L.) plants of forage- and turf-type cultivars
F. Altpeter (2004)
10.1023/B:TICU.0000016823.94547.c7
Transgenic Plants of Colonial Bentgrass from Embryogenic Callus via Agrobacterium-mediated Transformation
M. Chai (2004)
10.1089/DNA.2005.24.670
In vitro plant regeneration and genetic transformation of Dichanthium annulatum.
J. Kumar (2005)
10.1111/j.1757-1707.2009.01010.x
The ecology and agronomy of Miscanthus sinensis, a species important to bioenergy crop development, in its native range in Japan: a review
J. R. Stewart (2009)
Production of herbicidetolerant zoysiagrass by Agrobacterium-mediated transformation
K Toyama (2003)
ブドウにおける新しい Embryogenic Callus 獲得法
井樋 昭宏 (1999)
10.1007/s00299-004-0900-1
Increased resistance to crown rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene
W. Takahashi (2004)
10.1046/J.1365-313X.1999.00464.X
Non-invasive quantitative detection and applications of non-toxic, S65T-type green fluorescent protein in living plants.
Y. Niwa (1999)
10.1007/s11240-010-9708-6
The effects of genotype, inflorescence developmental stage and induction medium on callus induction and plant regeneration in two Miscanthus species
K. Głowacka (2010)
10.5511/PLANTBIOTECHNOLOGY.24.533
Production of transgenic plants and their early seed set in Japanese soybean variety, Kariyutaka
H. Sato (2007)
In vitro propagation of Miscanthus
NJ Gawel (1990)
10.1111/J.1744-697X.2006.00053.X
Agrobacterium tumefaciens‐mediated transformation of forage‐type perennial ryegrass (Lolium perenne L.)
H. Sato (2006)
10.1007/BF00032967
Long-term effects of thidiazuron are intermediate between benzyladenine, kinetin or isopentenyladenine in Miscanthus sinensis
J. M. Nielsen (2004)
10.1023/A:1022854303371
Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis
Karen Koefoed Petersen (2004)
Transgenic perennial ryegrass plants
T Yamada (2004)
10.1007/s00299-001-0423-y
Transformation of bahiagrass (Paspalum notatum Flugge)
R. Smith (2001)
10.1111/j.1365-313X.2008.03457.x
Improvement of biomass through lignin modification.
X. Li (2008)
10.2135/CROPSCI2007.04.0195
Expression of cry1Fa in Bahiagrass Enhances Resistance to Fall Armyworm
G. Luciani (2007)
Grasses of Japan and its neighboring regions : an identification manual
小山 鐵夫 (1987)
Genetic and nongenetic factors influencing callus induction in Miscanthus sinensis (Anderss.) anther cultures
K. G 3 owacka
10.1016/J.INDCROP.2009.07.011
Polyploidization of Miscanthus sinensis and Miscanthus x giganteus by plant colchicine treatment
K. Głowacka (2009)
10.1007/978-94-011-0485-2_10
Somatic Embryogenesis in Herbaceous Dicots
D. Brown (1995)
Miscanthus productivity. In: Miscanthus for Energy and Fibre (eds Jones M
JC Clifton-Brown (2001)
Somatic Embryogenesis: Molecular Embryology of Flowering Plants, pp. 467–499
V Raghavan (1997)
10.1111/J.1399-3054.1962.TB08052.X
A revised medium for rapid growth and bio assays with tobacco tissue cultures
T. Murashige (1962)



This paper is referenced by
10.3389/fpls.2017.02221
Genetic Transformation System for Woody Plant Tripterygium wilfordii and Its Application to Product Natural Celastrol
Y. Zhao (2018)
10.25560/9630
Characterization of the Plant Cell Wall Response to Isoxaben induced Cell Wall Damage
Lars Kjaer (2011)
10.1007/s11240-016-1078-2
Plant regeneration from calli in Japanese accessions of Miscanthus
W. Takahashi (2016)
10.1155/2014/501016
Phenotypic Characterization of Transgenic Miscanthus sinensis Plants Overexpressing Arabidopsis Phytochrome B
Ok-Jin Hwang (2014)
10.1017/S147926211400094X
Miscanthus : a case study for the utilization of natural genetic variation
T. R. Hodkinson (2015)
10.1007/s11240-013-0419-7
Agrobacterium-mediated genetic transformation of Miscanthus sinensis
Ok-Jin Hwang (2013)
10.1007/s11240-018-1389-6
Establishment of Miscanthus sinensis with decreased lignin biosynthesis by Agrobacterium–mediated transformation using antisense COMT gene
J. Yoo (2018)
10.3389/fpls.2013.00217
Advances in the genetic dissection of plant cell walls: tools and resources available in Miscanthus
G. Slavov (2013)
10.1016/J.BIOMBIOE.2017.10.012
Effective and simple in vitro regeneration system of Miscanthus sinensis, M. × giganteus and M. sacchariflorus for planting and biotechnology purposes
A. Ślusarkiewicz-Jarzina (2017)
10.1186/s13068-019-1632-3
Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Panicum virgatum L.)
Christine A Ondzighi-Assoume (2019)
10.1016/S2095-3119(15)61181-9
Sweet sorghum and Miscanthus: Two potential dedicated bioenergy crops in China
Shi-wei Hu (2017)
10.1111/gcbb.12275
High‐density genetic map of Miscanthus sinensis reveals inheritance of zebra stripe
Siyao Liu (2016)
10.1111/j.1757-1707.2012.01206.x
Synthetic polyploid production of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus
Won Byoung Chae (2013)
10.1111/J.1744-697X.2011.00234.X
Establishment of plant regeneration system in Erianthus arundinaceus (Retz.) Jeswiet, a potential biomass crop.
N. Uwatoko (2011)
10.1007/s11240-012-0284-9
Shoot organogenesis in three Miscanthus species and evaluation for genetic uniformity using AFLP analysis
C. Rambaud (2012)
Dissecting key biomass traits through traditional QTL mapping, exploring novel functional genomics approaches, and investigating self-incompatibility in Miscanthus
Justin M. Gifford (2015)
10.1111/pbi.12764
Genetic engineering of grass cell wall polysaccharides for biorefining
R. Bhatia (2017)
10.1007/978-3-319-08714-6
Molecular Breeding of Forage and Turf
H. Budak (2015)
10.1007/978-94-007-5500-0_11
Biotechnology of Miscanthus
S. Dalton (2013)
10.1016/j.tplants.2015.10.002
Molecular Breeding for Improved Second Generation Bioenergy Crops.
M. Allwright (2016)
10.1007/978-1-4419-5947-8_4
The Gene Pool of Miscanthus Species and Its Improvement
E. J. Sacks (2013)
10.1007/s11240-020-01784-8
A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals
S. J. Dalton (2020)
10.1007/978-3-319-08714-6_8
Candidate Gene Approach in Miscanthus spp. for Biorefinery
T. Yamada (2015)
10.46526/pccm.2019.v15i2.139
Sterilization procedures and Plant Preservative Mixture on in vitro establishment of Miscanthus sinensis Andersson
A. Lédo (2020)
EFFECT OF GENOTYPE AND INFLORESCENCE DEVELOPMENTAL STAGE ON CALLUS INDUCTION AND PLANT REGENERATION OF MISCANTHUS LUTARIORIPARIU , A NON-FOOD BIOMASS CROP
Lingling Zhao (2016)
10.2478/prolas-2020-0032
Induction of Polyploidy in Giant Miscanthus (Miscanthus × Giganteus Greef Et Deu.)
O. Melnychuk (2020)
10.1007/978-81-322-3763-1_18
Recent Progress in the Genetic Engineering of Biofuel Crops
K. Akashi (2018)
10.1007/978-3-319-44530-4_11
Creation of Novel Tetraploid Miscanthus sinensis Genotypes
C. Münnich (2016)
10.3390/agronomy9110673
Breeding Strategies to Improve Miscanthus as a Sustainable Source of Biomass for Bioenergy and Biorenewable Products
J. Clifton-Brown (2019)
10.1007/s12892-017-0175-0
Breeding of Lignocellulosic Bioethanol Feedstock
Yong Suk Chung (2017)
10.5772/31889
Molecular Breeding of Grasses by Transgenic Approaches for Biofuel Production
W. Takahashi (2012)
10.1139/cjps-2018-0168
Transgenic Miscanthus lutarioriparius that co-expresses the Cry 2Aa# and Bar genes
Y. Xia (2019)
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