A Novel Cysteine‐rich Receptor‐like Kinase Gene, TaCRK2, Contributes To Leaf Rust Resistance In Wheat
Jia Gu, Jiawei Sun, N. Liu, Xizhe Sun, C. Liu, L. Wu, G. Liu, Fanli Zeng, C. Hou, S. Han, Wenchao Zhen, D. Wang
Published 2020 · Medicine, Biology
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Abstract Leaf rust, caused by Puccinia triticina, is one of the most destructive fungal diseases in wheat production worldwide. The hypersensitive reaction (HR) is an important defence response against P. triticina infection. In this study, the physiological races 165 and 260 of P. triticina were combined with a line derived from the bread wheat cultivar Thatcher with the leaf rust resistance locus Lr26 to form compatible and incompatible combinations, respectively. Based on an RNA‐Seq database of the interaction systems, a new wheat cysteine‐rich receptor‐like kinase gene, TaCRK2, is specifically induced and up‐regulated in the incompatible combination. We identified that TaCRK2 was regulated in a Ca2+‐dependent manner. Knockdown of TaCRK2 by virus‐induced gene silencing and RNAi leads to a dramatic increase in HR area and the number of haustorial mother cells at the single infection site. In addition, urediniospores, a P. triticina‐specific pathogenic marker in compatible combinations, were observed on leaf surfaces of silenced plants at approximately 15 days after inoculation in the incompatible combination. Moreover, transcription levels of TaPR1, TaPR2, and TaPR5 were obviously reduced in TaCRK2‐silenced plants. TaCRK2 overexpression in Nicotiana benthamiana induced strong HR‐like cell death. Finally, transient expression of green fluorescent protein fused with TaCRK2 in N. benthamiana indicated that TaCRK2 localizes in the endoplasmic reticulum. Thus, TaCRK2 plays an important role in the resistance to P. triticina infection and has a positive regulation effect on the HR cell death process induced by P. triticina.
This paper references
Of PAMPs and Effectors: The Blurred PTI-ETI Dichotomy[OA]
B. Thomma (2011)
Integrative Genomics Viewer
J. Robinson (2011)
TaCAMTA4, a Calmodulin-Interacting Protein, Involved in Defense Response of Wheat to Puccinia triticina
Yuelin Wang (2019)
Development of a Virus-Induced Gene-Silencing System for Hexaploid Wheat and Its Use in Functional Analysis of the Lr21-Mediated Leaf Rust Resistance Pathway1
S. Scofield (2005)
Sensitization of defense responses and activation of programmed cell death by a pathogen-induced receptor-like protein kinase in Arabidopsis
Kegui Chen (2004)
Recognition and response in the plant immune system.
Z. Nimchuk (2003)
Mechanistic insights into the evolution of DUF26-containing proteins in land plants
Aleksia Vaattovaara (2019)
How plants recognize pathogens and defend themselves
P. D. Wit (2007)
Plant Protein Kinase Families and Signal Transduction
J. Stone (1995)
Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype
D. Kim (2019)
A Cysteine-Rich Protein Kinase Associates with a Membrane Immune Complex and the Cysteine Residues Are Required for Cell Death1[OPEN]
K. A. Yadeta (2016)
CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis
Atish Sardar (2017)
Convergent targeting of a common host protein-network by pathogen effectors from three kingdoms of life.
Ralf Weßling (2014)
Cysteine-rich receptor-like kinase CRK5 as a regulator of growth, development, and ultraviolet radiation responses in Arabidopsis thaliana
Paweł Burdiak (2015)
The Arabidopsis Cysteine-Rich Receptor-Like Kinase CRK36 Regulates Immunity through Interaction with the Cytoplasmic Kinase BIK1
Dong Sook Lee (2017)
A novel citrate synthase isoform contributes infection and stress resistance of the stripe rust fungus
D Li (2018)
Nucleo-cytoplasmic shuttling of VPg encoded by Wheat yellow mosaic virus requires association with the coat protein.
L. Sun (2013)
The role of effectors in nonhost resistance to filamentous plant pathogens
R. Stam (2014)
The Receptor-like Pseudokinase GHR1 Is Required for Stomatal Closure[OPEN]
Maija Sierla (2018)
Stripe Rust Effector PstGSRE1 Disrupts Nuclear Localization of ROS-Promoting Transcription Factor TaLOL2 to Defeat ROS-Induced Defense in Wheat.
Tuo Qi (2019)
TaADF7, an actin-depolymerizing factor, contributes to wheat resistance against Puccinia striiformis f. sp. tritici.
Y. Fu (2014)
Plant transmembrane receptors: new pieces in the signaling puzzle.
D. M. Braun (1996)
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
S. Hanks (1988)
Recent Advances in PAMP-Triggered Immunity against Bacteria: Pattern Recognition Receptors Watch over and Raise the Alarm1
Valérie Nicaise (2009)
MO25α/β interact with STRADα/β enhancing their ability to bind, activate and localize LKB1 in the cytoplasm
J. Boudeau (2003)
Isolation and characterization of a novel wheat cysteine-rich receptor-like kinase gene induced by Rhizoctonia cerealis
K. Yang (2013)
Calcium influx is required for the initiation of the hypersensitive response of Triticum aestivum to Puccinia recondita f.sp. tritici
Gang Liu (2010)
Enhanced Arabidopsis pattern-triggered immunity by overexpression of cysteine-rich receptor-like kinases
Yu-Hung Yeh (2015)
Changes of Nitric Oxide and Its Relationship with H2O2 and Ca2+ in Defense Interactions between Wheat and Puccinia Triticina
Mei Ying Qiao (2015)
The Effects of Ca~(2+) on the Induction of Defence Responses by Wheat Leaf Rusts
W. Dong-mei (2007)
Wheat Gene TaATG8j Contributes to Stripe Rust Resistance
Md Abdullah-Al Mamun (2018)
Arabidopsis EF-Tu receptor enhances bacterial disease resistance in transgenic wheat.
H. Schoonbeek (2015)
Overexpression of CRK13, an Arabidopsis cysteine-rich receptor-like kinase, results in enhanced resistance to Pseudomonas syringae.
B. Acharya (2007)
Silencing and Innate Immunity in Plant Defense Against Viral and Non-Viral Pathogens
A. Zvereva (2012)
Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins
C. Hervé (2004)
Regulation of basal resistance by a powdery mildew-induced cysteine-rich receptor-like protein kinase in barley.
Cbgowda Rayapuram (2012)
The Arabidopsis thaliana cysteine-rich receptor-like kinase CRK20 modulates host responses to Pseudomonas syringae pv. tomato DC3000 infection.
L. Ederli (2011)
Plant Pattern Recognition Receptors
Libo Shan (2017)
Identification and cloning of a bacterial-blight resistance-related gene OsCRK1 in rice
M W Zhang (2015)
Elicitor-induced [Ca 2+ ] cyt elevation mainly depends on Ca 2+ influx in mesophyll protoplasts of wheat
G Liu (2015)
Abiotic stress-inducible receptor-like kinases negatively control ABA signaling in Arabidopsis.
H. Tanaka (2012)
Plant cells under siege: plant immune system versus pathogen effectors.
S. Asai (2015)
Plant PRRs and the activation of innate immune signaling.
Alberto P. Macho (2014)
MEME Suite: tools for motif discovery and searching
T. Bailey (2009)
SCR96, a small cysteine-rich secretory protein of Phytophthora cactorum, can trigger cell death in the Solanaceae and is important for pathogenicity and oxidative stress tolerance.
Xiao-Ren Chen (2016)
Transcriptome analysis of wheat leaves infected by Puccinia triticina
N Liu (2019)
Specific Targeting of a Plasmodesmal Protein Affecting Cell-to-Cell Communication
C. Thomas (2008)
Cholesterol and the Golgi apparatus.
M. Bretscher (1993)
Barley stripe mosaic virus-induced gene silencing in a monocot plant.
S. Holzberg (2002)
Identification of barley stripe mosaic virus genes involved in viral RNA replication and systemic movement.
I. Petty (1990)
Molecular mechanism of BIR3 in regulating growth and disease-resistance of tomato and Arabidopsis
S H Huang (2018)
Virus-Induced Gene Silencing-Based Functional Characterization of Genes Associated with Powdery Mildew Resistance in Barley1
I. Hein (2005)
Perturbations of the ZED1 Pseudokinase Activate Plant Immunity
D. P. Bastedo (2019)
Programmed cell death: a way of life for plants.
J. Greenberg (1996)
A Novel Wheat C-bZIP Gene, TabZIP14-B, Participates in Salt and Freezing Tolerance in Transgenic Plants
L. Zhang (2017)
Endosperm protein of wheat seed as a determinant of seedling growth.
L. Lowe (1973)
The expression pattern of TaCDPKs in the interaction of wheat and Puccinia triticina
C Yan (2016)
Graphbased genome alignment and genotyping with HISAT2 and HISATgenotype
D Kim (2019)
Expression analysis of TaCBL gene in defense response of wheat to Puccinia triticina
Q Q Wang (2016)
Transcriptional regulation of the CRK/DUF26 group of Receptor-like protein kinases by ozone and plant hormones in Arabidopsis
Michael Wrzaczek (2009)
Wheat streak mosaic virus: a century old virus with rising importance worldwide.
K. Singh (2018)
A malectin-like/leucine-rich repeat receptor protein kinase gene, RLK-V, regulates powdery mildew resistance in wheat.
P. Hu (2018)
Purification, characterization, and molecular gene cloning of an antifungal protein from Ginkgo biloba seeds
Y. Sawano (2007)
Terrific Protein Traffic: The Mystery of Effector Protein Delivery by Filamentous Plant Pathogens
R. Panstruga (2009)
The role of drugs affecting calcium metabolism and calcium channel on HR induced by wheat leaf rust fungus
W. Dong-mei (2006)
Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation.
S. Yoshimura (1998)
[Advances of LKB1-AMPK-mTOR Signaling Pathway in Tumor].
Xia Zhang (2011)
The wheat calcium-dependent protein kinase TaCPK7-D positively regulates host resistance to sharp eyespot disease.
Xuening Wei (2016)
The Arabidopsis thaliana cysteine-rich receptor-like kinases CRK6 and CRK7 protect against apoplastic oxidative stress.
N. Idänheimo (2014)
Structure and function of the receptor-like protein kinases of higher plants
J. Walker (2004)
A renaissance of elicitors: perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors.
T. Boller (2009)
Identification of an Apoplastic Protein Involved in the Initial Phase of Salt Stress Response in Rice Root by Two-Dimensional Electrophoresis1[C][W][OA]
L. Zhang (2008)
Activation of hypersensitive cell death by pathogen-induced receptor-like protein kinases from Arabidopsis
Kegui Chen (2004)
The hypersensitive response; the centenary is upon us but how much do we know?
L. Mur (2008)
Novel cargo-binding site in the β and δ subunits of coatomer
K. Michelsen (2007)
Increased Non-Canonical Splicing of Autoantigen Transcripts Provides the Structural Basis for Expression of Untolerized Epitopes.
Xiaofeng Yang (2004)
The Destination for Single-Pass Membrane Proteins Is Influenced Markedly by the Length of the Hydrophobic Domain Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000620.
F. Brandizzi (2002)
[The generation of H2O2 in wheat hypersensitive reaction induced by leaf rust fungus].
Yan Qi (2008)
Functional characterization of the Wrab17 gene in the interaction process between wheat and Puccinia triticina.
Yang Gaoshan (2018)
Expression Analysis of TaCDPK2 Gene in the Process of Wheat Infected by Puccinia triticina
Wang Dong-mei (2012)
Physical Association of Arabidopsis Hypersensitive Induced Reaction Proteins (HIRs) with the Immune Receptor RPS2*
Yiping Qi (2011)
Phytopathogen effectors subverting host immunity: different foes, similar battleground.
D. Dou (2012)
The α- and β′-COP WD40 Domains Mediate Cargo-selective Interactions with Distinct Di-lysine Motifs
A. Eugster (2003)
Genome analyses of the wheat yellow (stripe) rust pathogen Puccinia striiformis f. sp. tritici reveal polymorphic and haustorial expressed secreted proteins as candidate effectors
D. Cantu (2012)
Plant proteases in the control of the hypersensitive response.
Jose Salguero-Linares (2019)