Online citations, reference lists, and bibliographies.
← Back to Search

Assessment Of Microbial Diversity In The Rhizosphere Of Pinus Roxburghii (Sarg.) And Bio-inoculant Potential Of Selected Pine Bacterial Isolates For Wheat Varieties Based On Cultureindependent And Culture-dependent Techniques.

I. Naz, A. Bano, M. Mirza
Published 2018 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Evidence is lacking regarding compatibility of pine bacteria as bio-inoculants for crops. The diversity and abundance of rhizosphere bacteria of Pinus roxburghii has never been investigated with simultaneous application of culture-dependent and culture-independent techniques. The present study was aimed to isolate, characterise, check the bio-inoculant potential of pine bacteria and assess rhizosphere bacterial diversity using culture-independent advanced approaches. Forty bacteria isolated from the rhizoplane of P. roxburghii growing in a cold climate at high altitude in Murree, were morphologically characterised; nine were identified by 16S rRNA sequence analyses and used in experiments. Diversity and abundance of the 16S rRNA gene and nifH gene in the rhizosphere was assessed by cloning, RFLP analysis, 454-amplicon pyrosequencing and qPCR. The bacterial isolates significantly improved dry weight of shoot, root, root area, IAA and GA3 content, number of grains plant-1 , weight of grains plant-1 in wheat varieties Chakwal-50 and Fareed-06 under axenic and field conditions. The number of 16S rRNA sequences (2979) identified by pyrosequencing shared similarity with 13 phyla of bacteria and archaea. The results confirm the existence of diverse bacteria of agricultural and industrial importance in the rhizosphere and compatibility of rhizoplane bacteria as bio-inoculants for wheat varieties.
This paper references
10.1128/AEM.59.3.695-700.1993
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
G. Muyzer (1993)
MOLECULAR CHARACTERIZATION OF RHIZOSPHERE BACTERIAL COMMUNITIES ASSOCIATED WITH WHEAT(TRITICUM AESTIVUM L.) CULTIVARS AT FLOWERING STAGE
I. Naz (2014)
10.1371/journal.pone.0042149
A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase
J. C. Gaby (2012)
10.1111/j.1747-0765.2005.tb00034.x
Detection of nifH Sequences in Sugarcane (Saccharum officinarum L.) and Pineapple (Ananas comosus [L.] Merr.)
Shotaro Ando (2005)
10.1128/MMBR.68.4.669-685.2004
Metagenomics: Application of Genomics to Uncultured Microorganisms
J. Handelsman (2004)
10.4067/S0718-95162015005000016
The stimulatory effects of L-tryptophan and plant growth promoting rhizobacteria (PGPR) on soil health and physiology of wheat
T. U. Hassan (2015)
Plant Growth Promoting Rhizobacteria: A Critical Review
B. Saharan (2011)
10.9734/IJPSS/2013/4233
Role of soil nitrogen for the conifers of the boreal forest : a critical review
Carlo Lupi (2013)
EFFECT OF PLANT GROWTH PROMOTING RHIZOBACTERIA ON GROWTH, NUTRIENT, ORGANIC ACID, AMINO ACID AND HORMONE CONTENT OF CAULIFLOWER (Brassica oleracea L. var. botrytis) TRANSPLANTS
M. Ekinci (2014)
10.1128/AEM.00062-07
Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
Q. Wang (2007)
10.1038/ismej.2009.43
Abundance, composition, diversity and novelty of soil Proteobacteria
A. Spain (2009)
The Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Germination, Seedling Growth and Yield of Maize
A. Gholami (2009)
10.1099/IJS.0.65100-0
Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog.
T. Pankratov (2007)
10.1111/j.1550-7408.1999.tb04612.x
Investigating Deep Phylogenetic Relationships among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis 1
S. Turner (1999)
10.1016/J.EJSOBI.2013.10.010
Nutritional content analysis of plant growth-promoting rhizobacteria species
A. Gunes (2014)
10.5897/AJMR12.1170
Isolation and identification of endophytic bacteria of bananas (Musa spp.) in Kenya and their potential as biofertilizers for sustainable banana production
C. Ngamau (2012)
10.1038/ismej.2010.58
Soil bacterial and fungal communities across a pH gradient in an arable soil
J. Rousk (2010)
10.1007/BF00029072
Tryptophan-dependent biosynthesis of auxins in soil
M. Sarwar (2004)
10.1128/AEM.71.7.4117-4120.2005
Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays
N. Fierer (2005)
10.1016/J.SOILBIO.2004.08.023
Composition of the microbial communities in the mineral soil under different types of natural forest
E. Hackl (2005)
Evaluating some salinity tolerant rhizobacterial strains to lentil production under salinity stress
M. Islam (2013)
10.1038/nbt1486
Next-generation DNA sequencing
J. Shendure (2008)
10.1007/BF00197324
Biosynthesis and metabolism of abscisic acid in tomato leaves infected with Botrytis cinerea
J. Kettner (2004)
10.1128/AEM.71.11.7292-7300.2005
Paenibacillus polymyxa Invades Plant Roots and Forms Biofilms
S. Timmusk (2005)
10.1038/ismej.2011.188
454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants
T. Zhang (2012)
10.1128/AEM.69.9.5603-5608.2003
Isolation and Identification of Actinobacteria from Surface-Sterilized Wheat Roots
Justin T. Coombs (2003)
Response of Wheat Growth Parameters to Co-Inoculation of Plant Growth Promoting Rhizobacteria (PGPR) and Different Levels of Inorganic Nitrogen and Phosphorus
Z. Saber (2012)
10.1128/AEM.70.7.4363-4366.2004
Liquid Serial Dilution Is Inferior to Solid Media for Isolation of Cultures Representative of the Phylum-Level Diversity of Soil Bacteria
L. Schoenborn (2004)
10.4141/P01-047
Bacillus cereus UW85 inoculation effects on growth, nodulation, and N accumulation in grain legumes: Controlled-environment studies
J. Vessey (2002)
10.1016/S0929-1393(00)00069-X
METHODS FOR ASSESSING THE COMPOSITION AND DIVERSITY OF SOIL MICROBIAL COMMUNITIES
G. T. Hill (2000)
Analysis of total flavonoids and phenolics in different fractions of bark and needle extracts of Pinus roxburghii and Pinus wallichiana
A. Maimoona (2011)
10.1007/s13205-013-0126-4
Isolation of a multi-trait plant growth promoting Brevundimonas sp. and its effect on the growth of Bt-cotton
V. Kumar (2013)
10.1371/journal.pbio.0060280
The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA Sequencing
L. Dethlefsen (2008)
10.1046/J.1462-2920.2003.00451.X
Nitrogenase gene diversity and microbial community structure: a cross-system comparison.
J. Zehr (2003)
10.4014/JMB.1005.05014
Genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan.
S. Mehnaz (2010)
10.1016/J.CROPRO.2010.04.008
Biological control of Macrophomina phaseolina by chemotactic fluorescent Pseudomonas aeruginosa PN1 and its plant growth promotory activity in chir-pine
N. Singh (2010)
10.22069/IJPP.2014.1523
Rhizobacteria for reduced fertilizer inputs in wheat (Triticum aestivum spp. vulgare) and barley (Hordeum vulgare) on Aridisols in Turkey
R. Çakmakçı (2014)
10.3923/AJAR.2015.113.122
Impact of seed bacterization with PGPR on growth and nutrient uptake in different cultivable varieties of green gram
G. P. Kumar (2015)
10.1073/PNAS.0507535103
The diversity and biogeography of soil bacterial communities.
N. Fierer (2006)
10.1016/J.SOILBIO.2008.03.009
Impact of allelochemical exuded from allelopathic rice on soil microbial community
Chuihua Kong (2008)
10.1002/JPLN.200390038
Yield response of wheat and barley to inoculation of plant growth promoting rhizobacteria at various levels of nitrogen fertilization
A. Öztürk (2003)
10.1007/S11274-008-9680-Z
Biological control of root rot fungus Macrophomina phaseolina and growth enhancement of Pinus roxburghii (Sarg.) by rhizosphere competent Bacillus subtilis BN1
N. Singh (2008)
Separation and determination of three kinds of plant hormones by high-performance liquid chromatography
J. C. Li (1994)
10.4067/S0718-95162013005000051
Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth
Bhavna V. Mohite (2013)
10.1128/AEM.68.6.3035-3045.2002
Empirical and Theoretical Bacterial Diversity in Four Arizona Soils
J. Dunbar (2002)
10.9734/BMRJ/2013/4206
Seasonal Population Dynamics of Rhizosphere and Non-rhizosphere Soil Microorganisms of Chir Pine Seedlings ( Pinus roxburghii Sarg.)
Anurag Yadav (2013)
10.4161/psb.3.4.5204
Plant growth promotion by Bacillus megaterium involves cytokinin signaling
Randy Ortiz-Castro (2008)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar