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Experimental And Theoretical Investigation Of Chain Length And Surface Coverage On Fouling Of Surface Grafted Polypeptoids
Andrea R. Statz, Jinghao Kuang, Chun-lai Ren, A. Barron, I. Szleifer, P. Messersmith
Published 2009 · Chemistry, Medicine
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Numerous strategies exist to prevent biological fouling of surfaces in physiological environments; the authors’ strategy focuses on the modification of surfaces with poly-N-substituted glycine oligomers (polypeptoids). The authors previously reported the synthesis and characterization of three novel polypeptoid polymers that can be used to modify titanium oxide surfaces, rendering the surfaces resistant to adsorption of proteins, to adhesion of mammalian and bacterial cells, and to degradation by common protease enzymes. In this study, they investigated the effect of polypeptoid chain length on the antifouling properties of the modified surfaces. For these experiments, they used poly(N-methoxyethyl) glycines with lengths between 10 and 50 repeat units and determined the influence of chain length on coating thickness and density as well as resistance to protein adsorption and cellular adhesion. Short-term protein resistance remained low for all polymers, as measured by optical waveguide light mode spectroscopy, while fibroblast adhesion after several weeks indicated reduced fouling resistance for the polypeptoid-modified surfaces with the shortest chain length polymer. Experimental observations were compared to predictions obtained from a molecular theory of polymer and protein adsorption. Good agreement was found between experiment and theory for the chain length dependence of peptoid grafting density and for protein adsorption as a function of peptoid grafting density. The theoretical predictions provide specific guidelines for the surface coverage for each molecular weight for optimal antifouling. The predictions show the relationship between polymer layer structure and fouling.
This paper references
Efficient method for the preparation of peptoids [oligo(N-substituted glycines)] by submonomer solid-phase synthesis
R. Zuckermann (1992)
The biocompatibility of crosslinkable copolymer coatings containing sulfobetaines and phosphobetaines.
S. L. West (2004)
Protein resistance of titanium oxide surfaces modified by biologically inspired mPEG-DOPA.
J. Dalsin (2005)
The blood compatibility catastrophe.
B. Ratner (1993)
Solid State Technol
J N Hilfiker
A functionalized poly(ethylene glycol)-based bioassay surface chemistry that facilitates bio-immobilization and inhibits non-specific protein, bacterial, and mammalian cell adhesion.
G. Harbers (2007)
Evaluation of the stability of nonfouling ultrathin poly(ethylene glycol) films for silicon-based microdevices.
S. Sharma (2004)
E-BJIOBN-4-003902 for HPLC and MALDI-MS characterization of polypeptoids. For more information on EPAPS
See Epaps Document No
Interfacial properties and protein resistance of nano-scale polysaccharide coatings
H. Griesser (2002)
Biofilms and the technological implications of microbial cell adhesion
J. Bryers (1994)
Protein adsorption on tethered polymer layers: effect of polymer chain architecture and composition
I. Szleifer (1997)
“Non‐Fouling” Oligo(ethylene glycol)‐ Functionalized Polymer Brushes Synthesized by Surface‐Initiated Atom Transfer Radical Polymerization
Hongwei Ma (2004)
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air–water interface
J. De Feijter (1978)
Oxidation of polyethylene glycols by alcohol dehydrogenase.
D. Herold (1989)
Adsorption of fibrinogen and lysozyme on silicon grafted with poly(2-methacryloyloxyethyl phosphorylcholine) via surface-initiated atom transfer radical polymerization.
W. Feng (2005)
Effect of chain density on inhibition of protein adsorption by poly(ethylene glycol) based coatings
Martin Malmsten (1998)
Plasma protein adsorption on titanium: comparative in situ studies using optical waveguide lightmode spectroscopy and ellipsometry
R. Kurrat (1998)
Reduced protein adsorption and platelet adhesion by controlled variation of oligomaltose surfactant polymer coatings.
M. Ruegsegger (2001)
Functionalized poly(ethylene glycol)-grafted polysiloxane monolayers for control of protein binding
N. Xia (2002)
Statistical thermodynamics of polymers near surfaces
I. Szleifer (1996)
A comparative study of protein adsorption on titanium oxide surfaces using in situ ellipsometry, optical waveguide lightmode spectroscopy, and quartz crystal microbalance/dissipation
Fredrik Höök (2002)
Poly-2-methyl-2-oxazoline: a peptide-like polymer for protein-repellent surfaces.
Rupert Konradi (2008)
Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry.
Andrea R. Statz (2008)
Optical grating coupler biosensors.
J. Vörös (2002)
Thermal/oxidative degradation and stabilization of polyethylene glycol
S. Han (1997)
Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline.
J. Waite (1981)
Hartree-Slater subshell photoionization cross-sections at 1254 and 1487 eV
J. Scofield (1976)
Poly(l-lysine)-graft-poly(ethylene glycol) Assembled Monolayers on Niobium Oxide Surfaces: A Quantitative Study of the Influence of Polymer Interfacial Architecture on Resistance to Protein Adsorption by ToF-SIMS and in Situ OWLS
S. Pasche (2003)
͑Color online͒ Total projected area of 3T3 fibroblasts during longterm cell culture on unmodified TiO 2 , Tyr-PMP1 10 , and PMP1 n modified TiO 2 substrates
Kinetic and thermodynamic control of protein adsorption.
J. Satulovsky (2000)
Biomimetic engineering of non-adhesive glycocalyx-like surfaces using oligosaccharide surfactant polymers
N. B. Holland (1998)
Protein adsorption on surfaces with grafted polymers: a theoretical approach.
I. Szleifer (1997)
Effects of cloud-point grafting, chain length, and density of PEG layers on competitive adsorption of ocular proteins.
P. Kingshott (2002)
Construction of a biomimetic surface on microfluidic chips for biofouling resistance.
Hongyan Bi (2006)
Effect of polysaccharide structure on protein adsorption
S. McArthur (2000)
P(AAm-co-EG) Interpenetrating Polymer Networks Grafted to Oxide Surfaces: Surface Characterization, Protein Adsorption, and Cell Detachment Studies
J. Bearinger (1997)
Spectroscopic ellipsometry for polymer thin films
J. Hilfiker (1998)
Exploiting the current paradigm of blood–material interactions for the rational design of blood-compatible materials
J. Brash (2000)
Novel tertiary amine oxide surfaces that resist nonspecific protein adsorption.
Suzanne J. Dilly (2006)
Kinetics of protein adsorption and desorption on surfaces with grafted polymers.
F. Fang (2005)
Bacterial oxidation of polyethylene glycol.
F. Kawai (1978)
Vinyl polymers based on L-histidine residues. Part 2. Swelling and electric behavior of smart poly(ampholyte) hydrogels for biomedical applications.
M. Casolaro (2006)
Single-molecule mechanics of mussel adhesion
H. Lee (2006)
Chemisorbed poly(propylene sulphide)-based copolymers resist biomolecular interactions
J. Bearinger (2003)
Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces.
J. Dalsin (2003)
Poly(l-lysine)-g-Poly(ethylene glycol) Layers on Metal Oxide Surfaces: Attachment Mechanism and Effects of Polymer Architecture on Resistance to Protein Adsorption†
G. Kenausis (2000)
Prevention of Protein Adsorption by Tethered Poly(ethylene oxide) Layers: Experiments and Single-Chain Mean-Field Analysis
T. McPherson (1998)
Copolymers including L-histidine and hydrophobic moiety for preparation of nonbiofouling surface.
Takehiko Ishii (2007)
Methods for reducing biosensor membrane biofouling.
Control of nanobiointerfaces generated from well-defined biomimetic polymer brushes for protein and cell manipulations.
R. Iwata (2004)
Effect of molecular structure on the adsorption of protein on surfaces with grafted polymers
F. Fang (2002)
Protein-rejecting ability of surface-bound dextran in end-on and side-on configurations: comparison to PEG.
E. Osterberg (1995)
Poly(ethylene oxide) Grafted to Silicon Surfaces: Grafting Density and Protein Adsorption.
Chapter 13 Principles underlying the role of adsorbed plasma proteins in blood interactions with foreign materials
T. Horbett (1993)
Microbial degradation of polyethers
F. Kawai (2001)
Polyphosphoprotein from the adhesive pads of Mytilus edulis.
J. Waite (2001)
Poly(N-acryloylsarcosine methyl ester) protein-resistant surfaces.
D. O. Teare (2005)
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Estefanía González Solveyra (2016)
The Effect of Molecular Structure on the Adsorption of PPO-PEO-PPO Triblock Copolymers on Solid Surfaces
Bingjing Lin (2013)
Self‐Assembled Multifunctional Polymers for Biointerfaces
G. Coullerez (2011)
Mussel-Inspired Adhesives and Coatings.
B. P. Lee (2011)
Polypeptoids: A perfect match for molecular definition and macromolecular engineering?
R. Luxenhofer (2013)
Tribological performance of aqueous copolymer lubricant in loaded contact with Si and coated Ti film
Bingjing Lin (2013)
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M. Li (2014)
Solid-Phase Synthesis of N-Substituted Glycine Oligomers (α-Peptoids) and Derivatives
A. Culf (2010)
Nonspecific interactions in biomedical applications
Vahid Adibnia (2020)
Peptoids for biomaterials science.
K. A. Lau (2014)
MUC1-Targeted Cancer Cell Photothermal Ablation Using Bioinspired Gold Nanorods
Daria C. Zelasko-Leon (2015)
Antifouling Peptoid Biointerfaces
V. Saxena (2020)
Universal surface-initiated polymerization of antifouling zwitterionic brushes using a mussel-mimetic peptide initiator.
Jinghao Kuang (2012)
Polypeptoids from N-Substituted Glycine N-Carboxyanhydrides: Hydrophilic, Hydrophobic, and Amphiphilic Polymers with Poisson Distribution
C. Fetsch (2011)
Polymer brushes on flat and curved surfaces: How computer simulations can help to test theories and to interpret experiments
K. Binder (2012)
2.3 Peptoids: Synthesis, Characterization, and Nanostructures
J. Seo (2017)
Lubrication performance of aqueous copolymer lubricants with phosphate ester additive at different temperature on the Si surface
Bingjing Lin (2020)
Surface Functionalization of Biomaterials by Poly(2‐oxazoline)s
Giulia Morgese (2017)
A survey of state-of-the-art surface chemistries to minimize fouling from human and animal biofluids.
Christophe Blaszykowski (2015)
Low Fouling, Peptoid-Coated Polysulfone Hollow Fiber Membranes—the Effect of Grafting Density and Number of Side Chains
Neda Mahmoudi (2019)
A Thermodynamic Description of the Adsorption of Simple Water-Soluble Peptoids to Silica.
Anna L. Calkins (2016)
PEG-mimetic peptoid reduces protein fouling of polysulfone hollow fibers.
Neda Mahmoudi (2017)
A negative correlation between water content and protein adsorption on polymer brushes.
W. Dai (2019)
Adsorption structure and tribological performance of aqueous copolymer lubricants on Si and Ti surfaces
Bingjing Lin (2014)
Tailored poly(2-oxazoline) polymer brushes to control protein adsorption and cell adhesion.
N. Zhang (2012)
Polyoxazolines for nonfouling surface coatings--a direct comparison to the gold standard PEG.
Rupert Konradi (2012)
Surface chemistry to minimize fouling from blood-based fluids.
Christophe Blaszykowski (2012)
Design, synthesis and use of peptoids in the diagnosis and treatment of cancer.
Arijit Bhowmik (2017)
Time dependence of lysozyme adsorption on end-grafted polymer layers of variable grafting density and length.
J. Genzer (2012)
2.204 – Peptoids: Synthesis, Characterization, and Nanostructures
J. Seo (2011)
Interfacial Assembly Inspired by Marine Mussels and Antifouling Effects of Polypeptoids: A Neutron Reflection Study
Fang Pan (2020)
Antifouling glycocalyx-mimetic peptoids.
H. O. Ham (2013)See more