Online citations, reference lists, and bibliographies.
← Back to Search

Critical Nitric Oxide Concentration For Pseudomonas Aeruginosa Biofilm Reduction On Polyurethane Substrates.

Bella H. Neufeld, Melissa M. Reynolds
Published 2016 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Bacterial colonies that reside on a surface, known as biofilms, are intrinsically impenetrable to traditional antibiotics, ultimately driving research toward an alternative therapeutic approach. Nitric oxide (NO) has gained attention for its biologically beneficial properties, particularly centered around its antibacterial capabilities. NO donors that can release the molecule under physiological conditions (such as S-nitrosothiols) can be utilized in clinical settings to combat bacterial biofilm infections. Herein the authors describe determining a critical concentration of NO necessary to cause >90% reduction of a Pseudomonas aeruginosa biofilm grown on medical grade polyurethane films. The biofilm was grown under optimal culture conditions [in nutrient broth media (NBM) at 37 °C] for 24 h before the addition of the NO donor S-nitrosoglutathione (GSNO) in NBM for an additional 24 h. The cellular viability of the biofilm after the challenge period was tested using varying concentrations of NO to determine the critical amount necessary to cause at least a 90% reduction in bacterial biofilm viability. The critical GSNO concentration was found to be 10 mM, which corresponds to 2.73 mM NO. Time kill experiments were performed on the 24 h biofilm using the critical amount of NO at 4, 8, 12, and 16 h and it was determined that the 90% biofilm viability reduction occurred at 12 h and was sustained for the entire 24 h challenge period. This critical concentration was subsequently tested for total NO release via a nitric oxide analyzer. The total amount of NO released over the 12 h challenge period was found to be 5.97 ± 0.66 × 10(-6) mol NO, which corresponds to 1.49 ± 0.17 μmol NO/ml NBM. This is the first identification of the critical NO concentration needed to elicit this biological response on a medically relevant polymer.
This paper references
Examination of bacterial resistance to exogenous nitric oxide.
Benjamin J. Privett (2012)
Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa.
N. Barraud (2006)
Accurate nitric oxide measurements from donors in cell media: identification of scavenging agents.
J. Harding (2014)
Kinetics of S-nitrosation processes in aqueous polymer solution for controlled nitric oxide loading: toward tunable biomaterials.
J. M. Joslin (2012)
Nitric oxide and the immune response
C. Bogdan (2001)
The chemistry of nitrosative stress induced by nitric oxide and reactive nitrogen oxide species. Putting perspective on stressful biological situations
L. Ridnour (2004)
Nitric oxide-releasing chitosan film for enhanced antibacterial and in vivo wound-healing efficacy.
Jong Oh Kim (2015)
Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms
N. Barraud (2009)
Nitric oxide-releasing chitosan oligosaccharides as antibacterial agents.
Yu-Ling Lu (2014)
Nitric oxide-releasing dendrimers as antibacterial agents.
B. Sun (2012)
Biofilms as complex differentiated communities.
P. Stoodley (2002)
S-Nitroso-N-acetylpenicillamine (SNAP) Impregnated Silicone Foley Catheters: A Potential Biomaterial/Device To Prevent Catheter-Associated Urinary Tract Infections
Alessandro Colletta (2015)
Nitric oxide release: part III. Measurement and reporting.
Peter N. Coneski (2012)
Self-produced exopolysaccharide is a signal that stimulates biofilm formation in Pseudomonas aeruginosa
Yasuhiko Irie (2012)
Nitric oxide release: part II. Therapeutic applications.
A. Carpenter (2012)
Some observations concerning the S-nitroso and S-phenylsulphonyl derivatives of L-cysteine and glutathione
T. Hart (1985)
Bacteriophage Cocktail for the Prevention of Biofilm Formation by Pseudomonas aeruginosa on Catheters in an In Vitro Model System
Weiling Fu (2009)
Bacterial biofilms: from the Natural environment to infectious diseases
L. Hall-Stoodley (2004)
Biofilm vs. planktonic bacterial mode of growth: which do human macrophages prefer?
E. Hernandez-Jimenez (2013)
Chemistry, analysis, and biological roles of S-nitrosothiols.
A. Butler (1997)
Plasma-enhanced synthesis of bioactive polymeric coatings from monoterpene alcohols: a combined experimental and theoretical study.
K. Bazaka (2010)
Nanosilver as a new generation of nanoproduct in biomedical applications.
K. Chaloupka (2010)
Nitric oxide: a key mediator of biofilm dispersal with applications in infectious diseases.
N. Barraud (2015)
A novel nitric oxide producing probiotic patch and its antimicrobial efficacy: preparation and in vitro analysis
Mitchell Lawrence Jones (2010)
Comparative evaluation of biofilm disinfectant efficacy tests.
K. Buckingham-Meyer (2007)
Anti-Biofilm Efficacy of Dual-Action Nitric Oxide-Releasing Alkyl Chain Modified Poly(amidoamine) Dendrimers.
B. Worley (2015)
Antibiotic resistance in Pseudomonas aeruginosa and alternative therapeutic options.
M. Chatterjee (2016)
S-Nitrosothiol-modified nitric oxide-releasing chitosan oligosaccharides as antibacterial agents.
Yu-Ling Lu (2015)
S-Nitrosated biodegradable polymers for biomedical applications: synthesis, characterization and impact of thiol structure on the physicochemical properties
Vinod B. Damodaran (2012)
Antibacterial iodine-supported titanium implants.
T. Shirai (2011)
Homogeneous chemiluminescent measurement of nitric oxide with ozone. Implications for continuous selective monitoring of gaseous air pollutants
A. Fontijn (1970)
Infections Associated with Medical Devices
C. Eiff (2012)
Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials.
A. L. Spoering (2001)
Antibacterial Coatings: Challenges, Perspectives, and Opportunities.
M. Cloutier (2015)
Perspectives series: host/pathogen interactions. Mechanisms of nitric oxide-related antimicrobial activity.
F. Fang (1997)
Functional gold nanoparticles for the storage and controlled release of nitric oxide: applications in biofilm dispersal and intracellular delivery.
Hien T. T. Duong (2014)
Antibacterial properties of nitric oxide-releasing sol-gel microarrays.
Kevin P Dobmeier (2004)
The antibiotic resistome: the nexus of chemical and genetic diversity
G. Wright (2007)
The Calgary Biofilm Device: New Technology for Rapid Determination of Antibiotic Susceptibilities of Bacterial Biofilms
H. Ceri (1999)
Nitric oxide-releasing polysaccharide derivative exhibits 8-log reduction against Escherichia coli, Acinetobacter baumannii and Staphylococcus aureus.
A. Pegalajar-Jurado (2015)
Intravascular Catheter-Related Bloodstream Infection
H. Shah (2013)
Nitric oxide releasing Tygon materials: studies in donor leaching and localized nitric oxide release at a polymer-buffer interface.
J. M. Joslin (2013)
Risk factors for the isolation of multi-drug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa: a systematic review of the literature.
M. Falagas (2006)
Nitric oxide donors: chemical activities and biological applications.
P. Wang (2002)
Prevention of bacterial biofilms by covalent immobilization of peptides onto plasma polymer functionalized substrates
C. Vreuls (2010)
Bactericidal efficacy of nitric oxide-releasing silica nanoparticles.
Evan M. Hetrick (2008)
The Chemistry of S-Nitrosothiols
D. Williams (1999)
Antibacterial nitric oxide-releasing xerogels: cell viability and parallel plate flow cell adhesion studies.
Evan M. Hetrick (2007)
Cationic Antimicrobial Polymers and Their Assemblies
A. M. Carmona-Ribeiro (2013)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar