Online citations, reference lists, and bibliographies.
← Back to Search

Laser Transfection With Gold Nanoparticles: Current State And New Particle Structures As A Perspective

S. Kalies, Lara Gentemann, Georgios C. Antonopoulos, M. S. Rakoski, D. Heinemann, M. Schomaker, T. Ripken, Heiko Meyer
Published 2015 · Materials Science, Engineering

Cite This
Download PDF
Analyze on Scholarcy
Share
Laser-based transfection techniques have gained significant interest during the last decade. Either single cell manipulation by focusing on the cell membrane or high-throughput can be realized with laser transfection. The latter is for example provided by gold nanoparticle mediated laser transfection. It is based on the heating of gold nanoparticles through laser irradiation, which permeabilizes the membrane. This technique satisfies most prerequisites of a reliable transfection technique, like efficiency and minimal cell impact. In order to bring it closer to routine usage, we investigated new particle configurations for gold nanoparticle mediated laser transfection. Our setup employs a 532 nm and 850 ps laser system. We immobilized gold particles on cell culture surfaces or modified silica particles with a gold particle surface coverage. Furthermore, first experiments achieving cell perforation with an organic nanoparticle based on polypyrrole were conducted. These three options achieved comparable efficiencies to the incubation of cells with free gold nanoparticles. With regard to the underlying mechanisms of perforation, we performed fluorescence microscopy based imaging of the cell state combined with holographic imaging directly after perforation. First results indicated a power dependent ion (calcium) and volume exchange with the extracellular medium in the first two minutes after perforation. In conclusion, our results can pave the way to a safer and more efficient way of high-throughput laser transfection with gold nanoparticles.
This paper references
10.1002/adma.201202625
In vitro and in vivo near-infrared photothermal therapy of cancer using polypyrrole organic nanoparticles.
K. Yang (2012)
10.1063/1.1397255
Generation of calcium waves in living cells by pulsed-laser-induced photodisruption
N. Smith (2001)
10.1371/journal.pone.0058604
Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down
D. Heinemann (2013)
10.1021/nn5017742
Comparison of gold nanoparticle mediated photoporation: vapor nanobubbles outperform direct heating for delivering macromolecules in live cells.
R. Xiong (2014)
10.1002/jbio.201400065
Biophysical effects in off-resonant gold nanoparticle mediated (GNOME) laser transfection of cell lines, primary- and stem cells using fs laser pulses.
M. Schomaker (2015)
10.3788/COL20090710.0898
Laser-based transfection with conjugated gold nanoparticles
C. Yao (2009)
10.1039/SA9731000123
Correspondence on Amalgamation
M. Chen (1973)
10.1364/BOE.5.002686
Surface modification of silica particles with gold nanoparticles as an augmentation of gold nanoparticle mediated laser perforation
S. Kalies (2014)
10.1039/c2cc34463g
Polypyrrole nanoparticles for high-performance in vivo near-infrared photothermal cancer therapy.
M. Chen (2012)
10.1117/1.JBO.19.7.070505
Immobilization of gold nanoparticles on cell culture surfaces for safe and enhanced gold nanoparticle-mediated laser transfection
S. Kalies (2014)
Targeted transfection by femtosecond laser
U. K. Tirlapur (2002)
10.1007/BF00697702
A novel method of DNA transfection by laser microbeam cell surgery
M. Tsukakoshi (1984)



Semantic Scholar Logo Some data provided by SemanticScholar